摘要
目的建立测定肝精补血素口服液中党参炔苷含量的高效液相色谱法。方法色谱柱为Agilent C18柱(250 mm×4. 6 mm,5μm),流动相为乙腈-0. 4%醋酸(22∶78,V/V),流速为1. 0 m L/min,检测波长为267 nm,柱温为30℃,进样量为10μL。结果党参炔苷进样量在37. 72~565. 80 ng(r=0. 999 5)范围内与峰面积线性关系良好,平均回收率为96. 52%,RSD为1. 60%(n=6),精密度、稳定性、重复性试验的RSD均小于2%。结论该方法操作简便、准确,精密度、稳定性、重复性均良好,可用于测定肝精补血素口服液中党参炔苷的含量。
Objective To establish an HPLC method for the content determination of lobetyolin in Ganjing Buxuesu Oral Liquid. Methods The chromatography column was Agilent C18 column( 250 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-0. 4 % acetic acid( 22 ∶ 78,V/V),the flow rate was 1. 0 m L/min,the detection wavelength was 267 nm,the column temperature was 30 ℃,and the sample size was 10 μL. Results The lobetyolin showed good linearity in the range of 37. 72-565. 80 ng( r = 0. 999 5),the average recovery rate was 96. 52%,RSD was 1. 60%( n = 6). RSDs of precision,stability and repeatability tests were less than 2 %.Conclusion The method is simple and accurate,it has good precision,stability and repeatability,which can be used for the content determination of lobetyolin in Ganjing Buxuesu Oral Liquid.
引文
[1]WS3-B-2530-97,卫生部药品标准·中药成方制剂(第十三册)[S].
[2]袁卫梅,吴生谦.肝精补血素口服液的药效学研究[J].中国药业,2006,15(17):29-30.
[3]张晓丹,刘琳,佟欣.党参、黄芪对中枢神经系统作用的比较研究[J].中草药,2003,34(9):822-823.
[4]宋丹.川党参活性成分与品质评价研究[D].上海:上海中医药大学,2008.
[5]朱恩圆,贺庆,王峥涛,等.党参化学成分研究[J].中国药科大学学报,2001,32(2):94-95.
[6]宋英,周小初,宋崎,等.党参中多糖的含量测定方法研究[J].中国药业,2008,17(23):9-10.
[7]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:281.
[8]汪胜,王先教,邓芳.复方阿胶补血颗粒中不同来源党参的薄层色谱鉴别[J].中国药业,2008,17(10):32.
[9]黄健,车晓平,陈志峰,等.不同产地党参中党参炔苷的含量测定[J].北京中医药,2011,30(6):467-468.
[10]赵晓华,刘养清,王润生,等.党参不同部位中党参炔苷的RP-HPLC分析[J].中成药,2007,29(7):1046-1047.
[11]孙庆文,黄敏,何顺志.HPLC测定7种党参类植物中的党参炔苷[J].华西药学杂志,2009,24(3):290-292.