高效液相色谱法测定肝精补血素口服液中党参炔苷含量
|
摘要
目的建立测定肝精补血素口服液中党参炔苷含量的高效液相色谱法。方法色谱柱为Agilent C18柱(250 mm×4. 6 mm,5μm),流动相为乙腈-0. 4%醋酸(22∶78,V/V),流速为1. 0 m L/min,检测波长为267 nm,柱温为30℃,进样量为10μL。结果党参炔苷进样量在37. 72～565. 80 ng(r=0. 999 5)范围内与峰面积线性关系良好,平均回收率为96. 52%,RSD为1. 60%(n=6),精密度、稳定性、重复性试验的RSD均小于2%。结论该方法操作简便、准确,精密度、稳定性、重复性均良好,可用于测定肝精补血素口服液中党参炔苷的含量。
Objective To establish an HPLC method for the content determination of lobetyolin in Ganjing Buxuesu Oral Liquid. Methods The chromatography column was Agilent C18 column( 250 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-0. 4 % acetic acid( 22 ∶ 78,V/V),the flow rate was 1. 0 m L/min,the detection wavelength was 267 nm,the column temperature was 30 ℃,and the sample size was 10 μL. Results The lobetyolin showed good linearity in the range of 37. 72-565. 80 ng( r = 0. 999 5),the average recovery rate was 96. 52%,RSD was 1. 60%( n = 6). RSDs of precision,stability and repeatability tests were less than 2 %.Conclusion The method is simple and accurate,it has good precision,stability and repeatability,which can be used for the content determination of lobetyolin in Ganjing Buxuesu Oral Liquid.
Objective To establish an HPLC method for the content determination of lobetyolin in Ganjing Buxuesu Oral Liquid. Methods The chromatography column was Agilent C18 column( 250 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-0. 4 % acetic acid( 22 ∶ 78,V/V),the flow rate was 1. 0 m L/min,the detection wavelength was 267 nm,the column temperature was 30 ℃,and the sample size was 10 μL. Results The lobetyolin showed good linearity in the range of 37. 72-565. 80 ng( r = 0. 999 5),the average recovery rate was 96. 52%,RSD was 1. 60%( n = 6). RSDs of precision,stability and repeatability tests were less than 2 %.Conclusion The method is simple and accurate,it has good precision,stability and repeatability,which can be used for the content determination of lobetyolin in Ganjing Buxuesu Oral Liquid.
引文
[1]WS3-B-2530-97,卫生部药品标准·中药成方制剂(第十三册)[S].
[2]袁卫梅,吴生谦.肝精补血素口服液的药效学研究[J].中国药业,2006,15(17):29-30.
[3]张晓丹,刘琳,佟欣.党参、黄芪对中枢神经系统作用的比较研究[J].中草药,2003,34(9):822-823.
[4]宋丹.川党参活性成分与品质评价研究[D].上海:上海中医药大学,2008.
[5]朱恩圆,贺庆,王峥涛,等.党参化学成分研究[J].中国药科大学学报,2001,32(2):94-95.
[6]宋英,周小初,宋崎,等.党参中多糖的含量测定方法研究[J].中国药业,2008,17(23):9-10.
[7]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:281.
[8]汪胜,王先教,邓芳.复方阿胶补血颗粒中不同来源党参的薄层色谱鉴别[J].中国药业,2008,17(10):32.
[9]黄健,车晓平,陈志峰,等.不同产地党参中党参炔苷的含量测定[J].北京中医药,2011,30(6):467-468.
[10]赵晓华,刘养清,王润生,等.党参不同部位中党参炔苷的RP-HPLC分析[J].中成药,2007,29(7):1046-1047.
[11]孙庆文,黄敏,何顺志.HPLC测定7种党参类植物中的党参炔苷[J].华西药学杂志,2009,24(3):290-292.
[2]袁卫梅,吴生谦.肝精补血素口服液的药效学研究[J].中国药业,2006,15(17):29-30.
[3]张晓丹,刘琳,佟欣.党参、黄芪对中枢神经系统作用的比较研究[J].中草药,2003,34(9):822-823.
[4]宋丹.川党参活性成分与品质评价研究[D].上海:上海中医药大学,2008.
[5]朱恩圆,贺庆,王峥涛,等.党参化学成分研究[J].中国药科大学学报,2001,32(2):94-95.
[6]宋英,周小初,宋崎,等.党参中多糖的含量测定方法研究[J].中国药业,2008,17(23):9-10.
[7]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:281.
[8]汪胜,王先教,邓芳.复方阿胶补血颗粒中不同来源党参的薄层色谱鉴别[J].中国药业,2008,17(10):32.
[9]黄健,车晓平,陈志峰,等.不同产地党参中党参炔苷的含量测定[J].北京中医药,2011,30(6):467-468.
[10]赵晓华,刘养清,王润生,等.党参不同部位中党参炔苷的RP-HPLC分析[J].中成药,2007,29(7):1046-1047.
[11]孙庆文,黄敏,何顺志.HPLC测定7种党参类植物中的党参炔苷[J].华西药学杂志,2009,24(3):290-292.