UHPLC-MS/MS法同时测定扭肚藤中5种活性成分的含量

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英文篇名：Simultaneous determination of five components in Jasminum elongatum by UHPLC-MS/MS 作者：胥爱丽 ; 陈昭 ; 毕晓黎 ; 江洁怡 ; 张靖年 ; 李素梅 ; 李养学 ; 陈伟韬 英文作者：XU Aili;CHEN Zhao;BI Xiaoli;JIANG Jieyi;ZHANG Jingnian;LI Sumei;LI Yangxue;CHEN Weitao;Guangdong Province Engineering Technology Research Institute of T.C.M (Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine); 关键词：扭肚藤 ; UHPLC-MS/MS ; 含量测定 英文关键词：Jasminum elongatum;;UHPLC-MS/MS;;simultaneous determination 中文刊名：GDYX 英文刊名：Journal of Guangdong Pharmaceutical University 机构：广东省中医药工程技术研究院(广东省中医药研究开发重点实验室); 出版日期：2019-05-27 11:12 出版单位：广东药科大学学报 年：2019 期：v.35;No.152 基金：广东省科技计划项目(2017A070701017) 语种：中文; 页：GDYX201903013 页数：5 CN：03 ISSN：44-1733/R 分类号：66-70

摘要

目的建立扭肚藤中5种活性成分质量分数同时测定的方法,更全面地评价扭肚藤药材的质量。方法采用UHPLC-MS/MS法,ESI离子源,Phenomenex gemini C_(18)(2.0 mm×50 mm,5μm)色谱柱,以乙腈(A)-0.1%(体积分数)甲酸(B)为流动相,梯度洗脱。结果建立了同时测定扭肚藤中咖啡酸、异绿原酸A、异绿原酸B、咖啡酸甲酯和水杨酸5种活性成分质量分数的方法,上述成分分别在0.001～0.2、0.2～16、0.1～8、0.001～0.1、0.01～1μg/m L范围内呈良好的线性关系,平均回收率分别为99.28%、95.36%、96.65%、98.27%、102.00%; RSD值分别为4.30%、1.89%、1.63%、3.40%、0.99%。结论本方法全面、准确、高效,能够更科学、系统地评价扭肚藤药材的质量。
        Objective To establish a method for the simultaneous determination of five components in Jasminum elongatum for the purpose of more precise evaluation of its quality. Methods UHPLC-MS/MS was used to determine five components in Jasminum elongatu. with a Phenomenex gemini C_(18)( 2.0 mm×50 mm,5 μm) by ESI source,and the mobile phase was acetonitrile -0. 1% formic acid with the gradient elution. Results A method was established for simultaneous determination of caffeic acid,methylcaffeate isochlorogenic acid A,isochlorogenic acid B and salicylic acid. They had a good linearity relationship between 0.001-0.2、0.2-16、0.1-8、0.001-0.1、0.01-1 μg/m L,and the average recoveries were 99.28%( RSD = 4.30%) 、95.36%( RSD =1.89%) 、96.65%( RSD = 1. 63%) 、98. 27%( RSD = 3. 40%) and 102. 00%( RSD = 0. 99%),respectively.Conclusion The established method was comprehensive,accurate and effective,which can be used for more scientific and systematic evaluation of Jasminum elongatum's quality.

引文

[1]JIA Qiang,SU Weiei,PENG Wei,et al.Anti-diarrhoea and analgesic activities of the methanol extract and its fractions of Jasminum amplexicaule Buch.-Ham.(Oleaceae)[J].JEthnopharmacol,2008,119(2):299-304.
    [2]李沛波,王永刚,彭维,等.复方扭肚藤胶囊对实验性腹泻的影响[J].中国中药杂志,2008,33(3):323-325.
    [3]GAO Zhenhua,YIN Junqiang,XIE Xiaolin,et al.Intracellular signaling mechanisms pharmacological action of Jasminum amplexicaule Buch.-Ham.(Oleaceae)on gastrointestinal secretion[J].Iran J Pharm Res,2014,13(3):959-965.
    [4]胥爱丽,毕晓黎,江洁怡,等.基于药效物质基础的扭肚藤药材质量评价研究[J].中成药,2018,40(3):632-637.
    [5]胥爱丽,陈雪,江洁怡,等.扭肚藤的化学成分研究[J].中药材,2016,39(8):1780-1782.
    [6]刘昌孝,陈士林,肖小河,等.中药质量标志物(Q-Marker):中药产品质量控制的新概念[J].中草药,2016,44(9):1443-1457.
    [7]张铁军,许浚,韩彦琪,等.中药质量标志物(Q-marker)研究:延胡索质量评价及质量标准研究[J].中草药,2016,44(9):1458-1467.
    [8]张铁军,许浚,申秀萍,等.基于中药质量标志物(Q-Marker)的元胡止痛滴丸的“性-效-物”三元关系和作用机制研究[J].中草药,2016,47(13):2199-2211.