不同丛枝菌根真菌种类对生姜生长的影响
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摘要
研究盆栽条件下,接种不同丛枝菌根真菌(arbuscular mycorrhizal fungi, AMF)种类对生姜不同生育时期农艺性状的影响及其在生姜根内的定殖情况。采用生姜组培苗,于幼苗期分别接种12株AMF进行生姜农艺性状比较试验,在温室盆栽接种后每隔30 d对生姜根系进行取样分析,比较菌根侵染率、侵染强度和丛枝丰度的大小,以观察不同AMF菌株对生姜苗的侵染动态。结果表明,所有接种AMF组的生姜植株,其生长量均高于空白对照组。结合不同菌株在根系的侵染率,筛选出生姜AMF优势种类为:细凹无梗囊霉(Acaulospora scrobiculata)、网状球囊霉(Glomus reticulatum)、隐形球囊霉(Paraglomus occltum)、摩西球囊霉(Glomus mosseae)。说明苗期接种这4个优势菌种,能够促进生姜生长,是适宜生姜生长的优良AMF菌株。
An experiment was conducted to analyze the effects of arbuscular mycorrhizal(AM) fungi on the growth of ginger(Zingiber officinale Roscoe) under greenhouse conditions. Ginger plantlets were inoculated with 12 different AM species, and the agronomic characters, the rate of colonization, infection intensity and arbuscular abundance were analyzed in every 30 days after inoculation. The results showed the ginger plantlets inoculated with AM fungi grew better than the ones without AM fungi. The dominant strains of AM fungi were Acaulospora scrobiculata, Glomus reticulatum, Paraglomus occltum and Glomus mosseae according to the growth simulation and infection in roots. It suggests that the inoculation of the four dominant strains improved the growth, yield and quality, and further research is needed to develop dominant strains.
An experiment was conducted to analyze the effects of arbuscular mycorrhizal(AM) fungi on the growth of ginger(Zingiber officinale Roscoe) under greenhouse conditions. Ginger plantlets were inoculated with 12 different AM species, and the agronomic characters, the rate of colonization, infection intensity and arbuscular abundance were analyzed in every 30 days after inoculation. The results showed the ginger plantlets inoculated with AM fungi grew better than the ones without AM fungi. The dominant strains of AM fungi were Acaulospora scrobiculata, Glomus reticulatum, Paraglomus occltum and Glomus mosseae according to the growth simulation and infection in roots. It suggests that the inoculation of the four dominant strains improved the growth, yield and quality, and further research is needed to develop dominant strains.
引文
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[15]刘贵猛,谭树朋,孙文献,等.AMF和PGPR对生姜青枯病的影响[J].菌物研究,2017,15(1):1-7.
[16]汪茜,张金莲,龙艳艳,等.广西柳江生姜根际土壤丛枝菌根真菌资源研究[J].西南农业学报,2016,29(1):115-119.
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[18]Millerwideman M A,Watrud L S.Sporulation of Gigaspora margarita on root cultures of tomato[J].Canadian Journal of Microbiology,1984,30(5):642-646.
[19]龙宣杞.丛枝菌根真菌(AMF)高效菌种的选育[D].天津:天津大学,2009.
[20]王颖.甘草植物根际AMF多样性及其生长效应的研究[D].呼和浩特:内蒙古大学,2008.
[21]齐国辉,陈贵林,吕桂云,等.丛枝菌根真菌对重茬草莓产量和品质的影响[J].果树学报,2001,18(6):341-344.
[22]王倡宪,李晓林,宋福强,等.两种丛枝菌根真菌对黄瓜苗期枯萎病的防效及根系抗病相关酶活性的影响[J].中国生态农业学报,2012,20(1):53-57.
[2]Hiiesalu I,Partel M,Davison J,et al.Species richness of arbuscular mycorrhizal fungi:Associations with grassland plant richness and biomass[J].New Phytologist,2014,203:233-244.
[3]Liu L L,Liu R D,Huang R H.Effect of arbuscular mycorrhizal fungi(AMF)on accumulation capability for Se and photosynthesis characteristics of Actinidia chinensis‘Hongyang’leaves[J].Science&Technology of Food Industry,2014,10:234-242.
[4]王瑾,毕银丽,张延旭,等.接种丛枝菌根对矿区扰动土壤微生物群落及酶活性的影响[J].南方农业学报,2014,45(8):1417-1423.
[5]Erik V,Wilfred F M R,Hannes A G,et al.Positive effects of organic farming on below-ground mutualists:large-scale comparison of mycorrhizal fungal communities in agricultural soils[J].New Phytologist,2010,186(4):968-979.
[6]Sikes B A,Cottenie C,Klironomos J N.Plant and fungal identity determines pathogen protection of plant roots by arbuscular mycorrhizas[J].Journal of Ecology,2009,97:1274-1280.
[7]Borowicz V.Do arbuscular mycorrhizal fungi alter plant-pathogen relations[J].Ecology,2001,82:3057-3068.
[8]Garg N,Chandel S.Arbuscular mycorrhizal networks:process and functions.A review[J].Agronomy for Sustainable Development,2010,30(3):581-599.
[9]Artursson V,Finlay R D,Jansson J K.Interactions between arbuscular mycorrhizal fungi and bacteria and their potential for stimulating plant growth[J].Environmental Microbiology,2006,8(1):1-10.
[10]曾维爱,龙世平,李宏光,等.苗期接种不同丛枝菌根真菌对烟草青枯病防治效果的影响[J].南方农业学报,2011,42(6):612-615.
[11]李海燕,刘润进,束怀瑞.丛枝菌根真菌提高植物抗病性的作用机制[J].菌物系统,2001,20(3):435-439.
[12]Yang W,Xu Q,Liu H X,et al.Evaluation of biological control agents against Ralstonia wilt on ginger[J].Biological Control,2012,62:144-151.
[13]陈婧怡.姜瘟病病原拮抗菌的筛选与鉴定[D].合肥:安徽农业大学,2017.
[14]王维华,李敏,刘润进,等.AMF对生姜某些生理指标的影响[J].青岛农业大学学报(自然科学版),2003,20(3):175-177.
[15]刘贵猛,谭树朋,孙文献,等.AMF和PGPR对生姜青枯病的影响[J].菌物研究,2017,15(1):1-7.
[16]汪茜,张金莲,龙艳艳,等.广西柳江生姜根际土壤丛枝菌根真菌资源研究[J].西南农业学报,2016,29(1):115-119.
[17]王幼珊,张淑彬,张美庆.中国丛枝菌根真菌资源与种质资源[M].北京:中国农业出版社,2011.
[18]Millerwideman M A,Watrud L S.Sporulation of Gigaspora margarita on root cultures of tomato[J].Canadian Journal of Microbiology,1984,30(5):642-646.
[19]龙宣杞.丛枝菌根真菌(AMF)高效菌种的选育[D].天津:天津大学,2009.
[20]王颖.甘草植物根际AMF多样性及其生长效应的研究[D].呼和浩特:内蒙古大学,2008.
[21]齐国辉,陈贵林,吕桂云,等.丛枝菌根真菌对重茬草莓产量和品质的影响[J].果树学报,2001,18(6):341-344.
[22]王倡宪,李晓林,宋福强,等.两种丛枝菌根真菌对黄瓜苗期枯萎病的防效及根系抗病相关酶活性的影响[J].中国生态农业学报,2012,20(1):53-57.