日粮中添加凝结芽孢杆菌对仔猪血浆抗氧化指标和肠黏膜生长的影响
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摘要
试验研究了日粮中添加凝结芽孢杆菌对仔猪血浆抗氧化指标和肠黏膜生长的影响。选取30头体重相近的21日龄健康断奶仔猪随机分为3组(对照组、试验Ⅰ组和试验Ⅱ组),每组10个重复,每个重复1头猪。对照组饲喂基础日粮,试验Ⅰ、Ⅱ组在基础日粮的基础上分别添加2.0×10~6、2.0×10~7 CFU/g的凝结芽孢杆菌。正试期21 d,试验结束时前腔静脉采血后屠宰取样。结果显示:与对照组相比,试验Ⅰ和Ⅱ组断奶仔猪血浆超氧化物歧化酶(SOD)活性、空肠和回肠绒毛高度与隐窝深度的比值、回肠总蛋白(TP)含量及RNA与DNA的比值均显著提高(P<0.05);试验Ⅱ组仔猪血浆丙二醛(MDA)含量显著降低(P<0.05);试验Ⅰ组空肠TP含量及TP/DNA显著提高(P<0.05)。在本试验条件下,日粮中添加凝结芽孢杆菌可以提高断奶仔猪的抗氧化能力,促进仔猪肠道黏膜细胞的增殖与更新,从而改善了肠道黏膜形态结构。
This study was conducted to determine the effects of dietary Bacillus coagulans supplementation on plasma antioxidant indexes and intestinal mucosal growth of piglets.Thirty healthy weaned piglets(21-day-old) were randomly divided into three experimental groups(10 replicates per group):Control group,group Ⅰ and group Ⅱ.The control group was fed the basal diet,and the experimental groups Ⅰ and Ⅱ were fed the basal diet+2.0×10~6 and 2.0×10~7 CFU/g of Bacillus coagulans,respectively.On day 21 of the trail,blood samples were collected and then all the piglets were sacrificed and intestinal mucosa were collected for analysis.The results showed that:Compared with control group,the plasma superoxide dismutase(SOD) activity,the ratio of villus height to crypt depth in jejunum and ileum,the ileal TP content and the ratios of RNA to DNA of pigs in both groups Ⅰand Ⅱ were significantly increased(P<0.05); The plasma malondialdehyde(MDA) content of pigs in group Ⅱ was significantly decreased(P<0.05), while the TP content and the ratio of TP to DNA in jejunum of pigs in group Ⅰ increased significantly(P<0.05).In conclusion,under the conditions of this experiment,dietary supplementation with Bacillus coagulans could enhance the antioxidant capacity of weaned piglets,promote the proliferation and renewal of intestinal mucosal cells,and improve the intestinal mucosal morphology.
This study was conducted to determine the effects of dietary Bacillus coagulans supplementation on plasma antioxidant indexes and intestinal mucosal growth of piglets.Thirty healthy weaned piglets(21-day-old) were randomly divided into three experimental groups(10 replicates per group):Control group,group Ⅰ and group Ⅱ.The control group was fed the basal diet,and the experimental groups Ⅰ and Ⅱ were fed the basal diet+2.0×10~6 and 2.0×10~7 CFU/g of Bacillus coagulans,respectively.On day 21 of the trail,blood samples were collected and then all the piglets were sacrificed and intestinal mucosa were collected for analysis.The results showed that:Compared with control group,the plasma superoxide dismutase(SOD) activity,the ratio of villus height to crypt depth in jejunum and ileum,the ileal TP content and the ratios of RNA to DNA of pigs in both groups Ⅰand Ⅱ were significantly increased(P<0.05); The plasma malondialdehyde(MDA) content of pigs in group Ⅱ was significantly decreased(P<0.05), while the TP content and the ratio of TP to DNA in jejunum of pigs in group Ⅰ increased significantly(P<0.05).In conclusion,under the conditions of this experiment,dietary supplementation with Bacillus coagulans could enhance the antioxidant capacity of weaned piglets,promote the proliferation and renewal of intestinal mucosal cells,and improve the intestinal mucosal morphology.
引文
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[8] 汪攀,易敢峰,孙自博,等.一株凝结芽孢杆菌的分离鉴定及益生性研究[J].中国畜牧兽医,2017,44(4):1195-1202.WANG P,YI G F,SUN Z B,et al.Isolation,identification and probiotic study of a strain of Bacillus coagulans [J].China Animal Husbandry & Veterinary Medicine,2017,44(4):1195-1202.(in Chinese)
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[10] KOHSAKA S,TAKAHASHI K,WANG L,et al.Inhibition of GSH synthesis potentiates temozolomide-induced bystander effect in glioblastoma[J].Cancer Letters,2013,331(5):68-75.
[11] ROMAGNOLI C,MARCUCCI G,FAVILLI F,et al.Role of GSH/GSSG redox couple in osteogenic activity and osteoclastogenic markers of human osteoblast-like Sa OS-2 cells[J].FEBS Journal,2013,280(6):867-879.
[12] LALLèS J P,BOSI P,SMIDT H,et al.Nutritional management of gut health in pigs around weaning[J].Proceedings of the Nutrition Society,2007,66(2):260-268.
[13] PLUSKE J R,THOMPSON M J,ATWOOD C S,et al.Maintenance of villus height and crypt depth,and enhancement of disaccharide digestion and monosaccharide absorption,in piglets fed on cows’ whole milk after weaning[J].Animal Science,2010,62(1):131-144.
[14] FASINA Y O,MORAN E T,ASHWELL C M,et al.Effect of dietary gelatin supplementation on the expression of selected enterocytegenes,intestinal development and early chick performance[J].International Journal of Poultry Science,2007,6(12):944-951.
[15] 刘涛,彭健,周诗其,等.外源性谷氨酰胺和谷氨酸对早期断奶仔猪肠粘膜形态、结构和小肠吸收功能及骨骼肌中DNA、RNA浓度的影响[J].中国兽医学报,2003,31(1):62-65.LIU T,PENG J,ZHOU S Q,et al.Effects of exogenous glutamine and glutamic acid on intestinal mucosal morphology,structure and intestinal absorption of early weaned piglets and DNA and RNA concentrations in skeletal muscle[J].Journal of Chinese Veterinary Science,2003,31(1):62-65.(in Chinese)
[16] 袁勤生.超氧化物歧化酶的研究现状及应用前景[J].中国药学杂志,1991,26(8):456-459.YUAN Q S.Research status and application prospect of superoxide dismutase[J].Chinese Journal of Pharmaceutical Sciences,1991,26(8):456-459.(in Chinese)
[17] 姚仕彬,叶元土,蔡春芳,等.丙二醛对离体草鱼肠道黏膜细胞的损伤作用[J].水生生物学报,2015,39(1):133-141.YAO S B,YE Y T,CAI C F,et al.Injury effects of malondialdehyde on intestinal mucosal cells of isolated grass carp [J].Journal of Hydrobiology,2015,39(1):133-141.(in Chinese)
[18] 武琳琳,帅宗文,胡子盈,等.髓过氧化物酶-抗中性粒细胞胞浆抗体相关性血管炎活动期血清标志物的研究[J].医学研究生学报,2015,28(4):406-410.WU L L,SHUAI Z W,HU Z Y,et al.Study on serum markers of myeloperoxidase-anti-neutrophil cytoplasmic antibody-associated vasculitis in active phase[J].Journal of Medical Postgraduates,2015,28(4):406-410.(in Chinese)
[19] 陈柏荣,危小良,彭湖,等.髓过氧化物酶与冠状动脉慢血流的相关性[J].中国动脉硬化杂志,2015,23(2):185-187.CHEN B R,WEI X L,PENG H,et al.Correlation between myeloperoxidase and coronary slow flow[J].Chinese Journal of Arteriosclerosis,2015,23(2):185-187.(in Chinese)
[20] MATESANZ N,LAFUENTE N,AZCUTIA V,et al.Xanthine oxidase-derived extracellular superoxide anions stimulate activator protein 1 activityand hypertrophy in human vascular smooth muscle via c-Jun N-terminal kinase and p38 mitogen-activated protein kinases[J].Hypertension,2007,25(4):609-618.
[21] 余文兰,王朵朵,郭剑英,等.硫氧还蛋白对过氧化氢诱导的BRL-3A细胞损伤的保护作用[J].中国畜牧兽医,2014,41(6):154-158.YU W L,WANG D D,GUO J Y,et al.Protective effect of thioredoxin on hydrogen peroxide-induced BRL-3A cell injury[J].China Animal Husbandry & Veterinary Medicine,2014,41(6):154-158.(in Chinese)
[22] CERA K R,MAHAN D C,CROSS R F,et al.Effect of age,weaning and postweaning diet on small intestinal growth and jejunal morphology in young swine[J].Journal of Animal Science,1988,66(2):574-584.
[23] ETHERIDGE R D,SEERLEY R W,WYATT R D.The effect of diet on performance,digestibility,blood composition and intestinal microflora of weaned pigs[J].Journal of Animal Science,1984,58(6):1396-140.
[24] JEURISSEN S H,LEWIS F,VAN DER KLIS J D,et al.Paramaters and techniques to determine intestinal health poultry as constitutedby immunity,integrity and functionality[J].Current Issues Intestinal Microbiology,2002,3(1):1-14.
[2] 阳坦,孙志洪,李晓敏.在早期断奶过程中氧化应激的产生机制及其影响[J].动物营养学报,2013,25(4):705-714.YANG T,SUN Z H,LI X M.Mechanism and effect of oxidative stress during early weaning[J].Journal of Animal Nutrition,2013,25(4):705-714.(in Chinese)
[3] 金立志.植物提取物添加剂在动物营养中的应用及其机制的研究进展[J].动物营养学报,2010,22(5):1154-1164.JIN L Z.Advances in application and mechanism of plant extract additives in animal nutrition[J].Chinese Journal of Animal Nutrition,2010,22(5):1154-1164.(in Chinese)
[4] 宫秀艳,刘顺德,韦明,等.凝结芽孢杆菌对感染产气荚膜梭菌肉鸡生长技能、肠道病变及免疫器官指数的影响[J].中国畜牧兽医,2015,42(2):478-486.GONG X Y,L S D,WEI M,et al.Effects of Bacillus coagulans on growth skills,intestinal lesions and immune organ index of Clostridium perfringens broilers[J].China Animal Husbandry & Veterinary Medicine,2015,42(2):478-486.(in Chinese)
[5] HYRONIMUS B,LE M C,URDACI M C.Coagulin,a bacteriocin-like inhibitory substance produced by Bacillus coagulans I4[J].Journal of Applied Microbiology,1998,95(1):42-50.
[6] JIN L Z,MARQUARDT R R,ZHAO X.A strain of Enterococcus faecium (18C23) inhibits adhesion of enterotoxigenic Escherichia coli K88 to porcine small intestine mucus[J].Applied & Environmental Microbiology,2000,66(10):4200-4204.
[7] EMAMI N K,DANESHMAND A,NAEINI S Z,et al.Effects of commercial organic acid blends on male broilers chanllenged with E.coli K88:Performance,microbiology,intestinal morphology,and immune response[J].Poultry Science,2017,96(9):3254-3263.
[8] 汪攀,易敢峰,孙自博,等.一株凝结芽孢杆菌的分离鉴定及益生性研究[J].中国畜牧兽医,2017,44(4):1195-1202.WANG P,YI G F,SUN Z B,et al.Isolation,identification and probiotic study of a strain of Bacillus coagulans [J].China Animal Husbandry & Veterinary Medicine,2017,44(4):1195-1202.(in Chinese)
[9] 张越,徐海旺,李鹏,等.凝结芽孢杆菌对断奶仔猪生长性能、肠道菌群和血液相关指标的影响[J].黑龙江畜牧兽医,2018,12:165-168.ZHANG Y,XU H W,LI P,et al.Effects of Bacillus coagulans on growth performance,intestinal flora and blood indexes of weaned piglets[J].Heilongjiang Animal Science and Veterinary Medicine,,2018,12:165-168.(in Chinese)
[10] KOHSAKA S,TAKAHASHI K,WANG L,et al.Inhibition of GSH synthesis potentiates temozolomide-induced bystander effect in glioblastoma[J].Cancer Letters,2013,331(5):68-75.
[11] ROMAGNOLI C,MARCUCCI G,FAVILLI F,et al.Role of GSH/GSSG redox couple in osteogenic activity and osteoclastogenic markers of human osteoblast-like Sa OS-2 cells[J].FEBS Journal,2013,280(6):867-879.
[12] LALLèS J P,BOSI P,SMIDT H,et al.Nutritional management of gut health in pigs around weaning[J].Proceedings of the Nutrition Society,2007,66(2):260-268.
[13] PLUSKE J R,THOMPSON M J,ATWOOD C S,et al.Maintenance of villus height and crypt depth,and enhancement of disaccharide digestion and monosaccharide absorption,in piglets fed on cows’ whole milk after weaning[J].Animal Science,2010,62(1):131-144.
[14] FASINA Y O,MORAN E T,ASHWELL C M,et al.Effect of dietary gelatin supplementation on the expression of selected enterocytegenes,intestinal development and early chick performance[J].International Journal of Poultry Science,2007,6(12):944-951.
[15] 刘涛,彭健,周诗其,等.外源性谷氨酰胺和谷氨酸对早期断奶仔猪肠粘膜形态、结构和小肠吸收功能及骨骼肌中DNA、RNA浓度的影响[J].中国兽医学报,2003,31(1):62-65.LIU T,PENG J,ZHOU S Q,et al.Effects of exogenous glutamine and glutamic acid on intestinal mucosal morphology,structure and intestinal absorption of early weaned piglets and DNA and RNA concentrations in skeletal muscle[J].Journal of Chinese Veterinary Science,2003,31(1):62-65.(in Chinese)
[16] 袁勤生.超氧化物歧化酶的研究现状及应用前景[J].中国药学杂志,1991,26(8):456-459.YUAN Q S.Research status and application prospect of superoxide dismutase[J].Chinese Journal of Pharmaceutical Sciences,1991,26(8):456-459.(in Chinese)
[17] 姚仕彬,叶元土,蔡春芳,等.丙二醛对离体草鱼肠道黏膜细胞的损伤作用[J].水生生物学报,2015,39(1):133-141.YAO S B,YE Y T,CAI C F,et al.Injury effects of malondialdehyde on intestinal mucosal cells of isolated grass carp [J].Journal of Hydrobiology,2015,39(1):133-141.(in Chinese)
[18] 武琳琳,帅宗文,胡子盈,等.髓过氧化物酶-抗中性粒细胞胞浆抗体相关性血管炎活动期血清标志物的研究[J].医学研究生学报,2015,28(4):406-410.WU L L,SHUAI Z W,HU Z Y,et al.Study on serum markers of myeloperoxidase-anti-neutrophil cytoplasmic antibody-associated vasculitis in active phase[J].Journal of Medical Postgraduates,2015,28(4):406-410.(in Chinese)
[19] 陈柏荣,危小良,彭湖,等.髓过氧化物酶与冠状动脉慢血流的相关性[J].中国动脉硬化杂志,2015,23(2):185-187.CHEN B R,WEI X L,PENG H,et al.Correlation between myeloperoxidase and coronary slow flow[J].Chinese Journal of Arteriosclerosis,2015,23(2):185-187.(in Chinese)
[20] MATESANZ N,LAFUENTE N,AZCUTIA V,et al.Xanthine oxidase-derived extracellular superoxide anions stimulate activator protein 1 activityand hypertrophy in human vascular smooth muscle via c-Jun N-terminal kinase and p38 mitogen-activated protein kinases[J].Hypertension,2007,25(4):609-618.
[21] 余文兰,王朵朵,郭剑英,等.硫氧还蛋白对过氧化氢诱导的BRL-3A细胞损伤的保护作用[J].中国畜牧兽医,2014,41(6):154-158.YU W L,WANG D D,GUO J Y,et al.Protective effect of thioredoxin on hydrogen peroxide-induced BRL-3A cell injury[J].China Animal Husbandry & Veterinary Medicine,2014,41(6):154-158.(in Chinese)
[22] CERA K R,MAHAN D C,CROSS R F,et al.Effect of age,weaning and postweaning diet on small intestinal growth and jejunal morphology in young swine[J].Journal of Animal Science,1988,66(2):574-584.
[23] ETHERIDGE R D,SEERLEY R W,WYATT R D.The effect of diet on performance,digestibility,blood composition and intestinal microflora of weaned pigs[J].Journal of Animal Science,1984,58(6):1396-140.
[24] JEURISSEN S H,LEWIS F,VAN DER KLIS J D,et al.Paramaters and techniques to determine intestinal health poultry as constitutedby immunity,integrity and functionality[J].Current Issues Intestinal Microbiology,2002,3(1):1-14.