五参顺脉胶囊的质量控制研究
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摘要
目的建立一种有效控制五参顺脉胶囊质量的含量测定方法。方法 HPLC同时测定人参皂苷Re、Rb1含量;色谱条件:Rapid Resolution(4.6 mm×100 mm,3.5-Micron)色谱柱,乙腈-0.1%磷酸水梯度洗脱,流速1.3 mL/min,检测波长203 nm,柱温30℃。结果人参皂苷Re、Rb1在该色谱条件下分离度良好,专属性强,可以准确地进行含量测定。结论该方法可以快速、准确、有效地对五参顺脉胶囊进行质量控制。
Objective To establish an effective method to control the quality of wushenshunmai capsule. Method HPLC determine the contents of ginsenoside Re, ginsenoside Rb1 simultaneously; Chromatographic conditions: Rapid Resolution(4.6 mm × 100 mm,3.5-Micron) column and acetonitrile-0.1% phosphoric acid-water gradient, flow rate 1.3 mL/min, detection wavelength 203 nm, column temperature was 30 ℃. Results Ginsenoside Re, ginsenoside Rb1 in the chromatographic separation conditions is good, specific, can accurately determine the content. Conclusion This method can quickly and accurately and efficiently control the quality of wushenshunmai capsules.
Objective To establish an effective method to control the quality of wushenshunmai capsule. Method HPLC determine the contents of ginsenoside Re, ginsenoside Rb1 simultaneously; Chromatographic conditions: Rapid Resolution(4.6 mm × 100 mm,3.5-Micron) column and acetonitrile-0.1% phosphoric acid-water gradient, flow rate 1.3 mL/min, detection wavelength 203 nm, column temperature was 30 ℃. Results Ginsenoside Re, ginsenoside Rb1 in the chromatographic separation conditions is good, specific, can accurately determine the content. Conclusion This method can quickly and accurately and efficiently control the quality of wushenshunmai capsules.
引文
[1]中国药典委员会.中国药典(一部)[S].北京:人民卫生出版社,2010:122.
[2]王燕,柴程芝,朱丹妮.HOLC-ELSD测定GSSM颗粒中酸枣仁皂苷A和人参皂苷Rb1[J].中国实验方剂学杂志,2011,17(12):76-78.
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[4]刘莉,程龙琼,周世玉.西洋参中人参皂苷Rg1人参皂苷Re和人参皂苷Rb1的含量测定[J].医药导报,2011,30(9):1208-1209.
[5]毕晓黎,罗文汇,李素梅,等.超高速液相测定西洋参中人参皂苷Rg1、Re、Rb1的含量研究[J].中国药师,2012,15(5):651-652.
[6]李静涛,邵丽华,巩飚,等.HPLC—ELSD法测定西洋参含片中人参皂苷Rg1、Re和Rbl的含量[J].药物分析杂志,2006,26(9):1289-1291.
[7]王旭,乌国庆,张晓茜.HPLC法测定进口西洋参中人参皂苷Rb1、Re、Rg1的含量[J].中国实验方剂学杂志,2004,10(6):27-29.
[8]张秀丽.人参与西洋参皂苷类成分的比较研究[J].中国中医药咨询,2012,4(5):46-47.
[9]李岚,陈华.HPLC法测定不同产地西洋参中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1含量[J].中医药导报,2011,8(20):103-105.
[10]杨立伟,郑传奇,蒋忠军,等.超高效液相色谱法测定西洋参中参皂苷Rg1、Re、Rh1的含量[J].中药材,2008,31(1):55-57.
[2]王燕,柴程芝,朱丹妮.HOLC-ELSD测定GSSM颗粒中酸枣仁皂苷A和人参皂苷Rb1[J].中国实验方剂学杂志,2011,17(12):76-78.
[3]莫炫永.HPLC测定参松养心胶囊中人参皂苷Rb1的含量[J].中国实验方剂学杂志,2012,18(12):112-114.
[4]刘莉,程龙琼,周世玉.西洋参中人参皂苷Rg1人参皂苷Re和人参皂苷Rb1的含量测定[J].医药导报,2011,30(9):1208-1209.
[5]毕晓黎,罗文汇,李素梅,等.超高速液相测定西洋参中人参皂苷Rg1、Re、Rb1的含量研究[J].中国药师,2012,15(5):651-652.
[6]李静涛,邵丽华,巩飚,等.HPLC—ELSD法测定西洋参含片中人参皂苷Rg1、Re和Rbl的含量[J].药物分析杂志,2006,26(9):1289-1291.
[7]王旭,乌国庆,张晓茜.HPLC法测定进口西洋参中人参皂苷Rb1、Re、Rg1的含量[J].中国实验方剂学杂志,2004,10(6):27-29.
[8]张秀丽.人参与西洋参皂苷类成分的比较研究[J].中国中医药咨询,2012,4(5):46-47.
[9]李岚,陈华.HPLC法测定不同产地西洋参中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1含量[J].中医药导报,2011,8(20):103-105.
[10]杨立伟,郑传奇,蒋忠军,等.超高效液相色谱法测定西洋参中参皂苷Rg1、Re、Rh1的含量[J].中药材,2008,31(1):55-57.