禽脑脊髓炎、鸡痘二联活疫苗安全性、最小免疫剂量及免疫效力试验
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摘要
为确定禽脑脊髓炎(AE)、鸡痘(AP)二联活疫苗的安全性、最小免疫剂量及免疫效力,自制3批二联活疫苗进行研究。结果显示,鸡接种10倍剂量疫苗后精神、食欲及发育均正常,临床表现和剖检均无异常;AEV YBF02株的最小免疫剂量为100 EID50,鸡痘鹌鹑化弱毒株的最小免疫剂量为10 EID50;3批疫苗AEV YBF02株的病毒含量均≥103.2EID50/羽份,鸡痘鹌鹑化弱毒株的病毒含量均≥103.4EID50/羽份,攻毒后保护指数为89~100。试验表明,自制二联活疫苗安全、有效、质量可控,可用于预防禽脑脊髓炎和鸡痘。
The safety,minimum immune dose,virus content and challenge-protection tests were conducted with three batches of the combined Avian Encephalomyelitis( AE) and Avian Pox( AP) live vaccine. The results showed that the spirit,appetite and growth level of the chickens inoculated with tenfold-dose vaccine were the same as those in control post inoculation,there was not any abnormal signs during clinical and autopsy examination;the minimum immune dose of the AEV( Strain YBF02) and the APV( Strain Quail-Adapted) were respectively 100 EID50 and 10 EID50;the virus content of the AEV(Strain YBF02) and APV(Strain Quail-Adapted)were respectively ≥103.2 EID50/bird and ≥103.4 EID50/bird;the protective index after challenging was 89 ~ 100.These results demonstrated that the safe,stable and effective vaccine is able to prevent poultry against AE and AP.
The safety,minimum immune dose,virus content and challenge-protection tests were conducted with three batches of the combined Avian Encephalomyelitis( AE) and Avian Pox( AP) live vaccine. The results showed that the spirit,appetite and growth level of the chickens inoculated with tenfold-dose vaccine were the same as those in control post inoculation,there was not any abnormal signs during clinical and autopsy examination;the minimum immune dose of the AEV( Strain YBF02) and the APV( Strain Quail-Adapted) were respectively 100 EID50 and 10 EID50;the virus content of the AEV(Strain YBF02) and APV(Strain Quail-Adapted)were respectively ≥103.2 EID50/bird and ≥103.4 EID50/bird;the protective index after challenging was 89 ~ 100.These results demonstrated that the safe,stable and effective vaccine is able to prevent poultry against AE and AP.
引文
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[11]李凯善.禽脑脊髓炎病毒YBF02毒株的毒力测定与种子批建立[D].西北农林科技大学,2012.Li K S.Avian Encephalomyelitis virus strain virulence YBF02determination to establish the seed lots[D].Northwest Agriculture and Forestry University,2012.
[2]王纯洁,马学恩,赵振华.禽脑脊髓炎研究进展[J].动物医学进展,2003,24(2):45-48.Wang C J,Ma X E,Zhao Z H.Research development of avian encephalomyelitis[J].Progress in Veterinary Medicine,2003,24(2):45-48.
[3]张淑霞,杨增岐,季芳,等.禽脑脊髓炎病毒感染雏鸡的病理组织学观察[J].中国兽医杂志,2009,45(11):26-28.Zhang S X,Yang Z Q,Ji F,et al.Histopathological observation of chicken infected with avian encephalomyelitis virus[J].Chinese Journal of Veterinary Medicine,2009,45(11):26-28.
[4]张淑霞,杨增歧,李金钧,等.禽脑脊髓炎的调查及病毒分离[J].中国兽医学报,2000,20(6):551-553.Zhang S X,Yang Z Q,Li J J,et al.Investigation and virus isolation of avian encephalomyelitis[J].Chinese Journal of Veterinary Science,2000,20(6):551-553.
[5]Calnek B W.Control of avian encephalomyelitis:A historical account[J].Avian Dis,1998,42(4):632-647.
[6]焦铁军,李河林,张浩,等.禽脑脊髓炎冻干活疫苗安全性、最小免疫剂量及免疫效力试验[J].西北农林科技大学学报(自然科学版),2006,34(12):25-28.Jiao T J,Li H L,Zhang H,et al.Safety and minimum immune dose and immune potency tests of avian encephalomyelitis live vaccines[J].Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition),2006,34(12):25-28.
[7]Calnek B W,Taylor P J,Sevoian M.Studies on avian encephalomyelitis.V.Development and application of an oral vaccine[J].Avian Dis,1961,5:297-312.
[8]秦卓明,何叶峰,刘伟,等.禽脑脊髓炎的研究进展和控制对策[J].中国禽业导刊,2002,19(1):18-20.Qin Z M,He Y F,Liu W,et al.Research progress and control strategy of avian encephalomyelitis[J].Guide to Chinese Poultry,2002,19(1):18-20.
[9]殷震,刘景华.动物病毒学[M].北京:科学出版社,1997.Yin Z,Liu J H.Animal virology[M].Beijing:Science Press,1997.
[10]Srinivasan V,Schnitzlein W M,Tripathy D N.A consideration of previously uncharacterized fowl poxvirus unidirectional and bidirectional late promoters for inclusion in homologous recombinant vaccines[J].Avian Dis,2003,47(2):286-295.
[11]李凯善.禽脑脊髓炎病毒YBF02毒株的毒力测定与种子批建立[D].西北农林科技大学,2012.Li K S.Avian Encephalomyelitis virus strain virulence YBF02determination to establish the seed lots[D].Northwest Agriculture and Forestry University,2012.