三氯六氨络合钴对DNA凝聚及解凝聚的影响
|
摘要
多价离子浓度的改变对DNA凝聚及解凝聚的发生具有一定作用,本文主要通过单分子磁镊及原子力显微镜研究三氯六氨络合钴浓度的改变对DNA凝聚形态和临界凝聚力的影响。随着三价离子浓度的增加,DNA的凝聚程度及临界凝聚力慢慢增强,但当三价离子浓度达到一定值之后,随着离子浓度的增加,DNA的凝聚形态变得更松散,而临界凝聚力也相应的减小,说明DNA解凝聚发生。探讨DNA和三氯六氨络合钴的相互作用的关系与机理,有助于定量研究三价离子浓度的改变对DNA凝聚形态及效率的影响,希望为研究基因传递及基因治疗的影响因素提供理论指导。
The change of multivalent ion concentration has a certain effect on DNA condensation and decondensation. In this paper,the effects of hexamine cobalt(Ⅲ) concentration on the DNA condensation morphology and critical condensing force were studied by the single molecule magnetic tweezers and atomic force microscopy. It was found that with the increase of the concentration of trivalent ions,the DNA condensation degree and critical force increased. But when the concentration reached a certain value,with the increase of the concentration of trivalent ions,the condensation morphology of DNA became looser and the critical condensing force decreased accordingly. This phenomenon indicated that DNA decondensation occurred. Exploring the relationship and mechanism of the interaction between DNA and hexamine cobalt(Ⅲ) is helpful to quantitatively study the influence of the concentration of hexamine cobalt(Ⅲ)on the morphology and efficiency of DNA condensation. It would provide theoretical guidance for studying the influencing factors of gene delivery and gene therapy.
The change of multivalent ion concentration has a certain effect on DNA condensation and decondensation. In this paper,the effects of hexamine cobalt(Ⅲ) concentration on the DNA condensation morphology and critical condensing force were studied by the single molecule magnetic tweezers and atomic force microscopy. It was found that with the increase of the concentration of trivalent ions,the DNA condensation degree and critical force increased. But when the concentration reached a certain value,with the increase of the concentration of trivalent ions,the condensation morphology of DNA became looser and the critical condensing force decreased accordingly. This phenomenon indicated that DNA decondensation occurred. Exploring the relationship and mechanism of the interaction between DNA and hexamine cobalt(Ⅲ) is helpful to quantitatively study the influence of the concentration of hexamine cobalt(Ⅲ)on the morphology and efficiency of DNA condensation. It would provide theoretical guidance for studying the influencing factors of gene delivery and gene therapy.
引文
[1]MANNING G S.The molecular theory of polyelectrolyte solutions with applications to the electrostatic properties of polynucleotides[J].Quarterly Reviews of Biophysics,1978,11(2):179-246.
[2]LASIC D D.Novel applications of liposomes[J].Trends in Biotechnology,1998,16(7):307-321.
[3]ZHOU J,KE F,LIANG D.Kinetic study on the reentrant condensation of oligonucleotide in trivalent salt solution[J].The Journal of Physical Chemistry B,2010,114(43):13675-13680.
[4]BESTEMAN K,VAN EIJK K,LEMAY S G.Charge inversion accompanies DNA condensation by multivalent ions[J].Nature Physics,2007,3(9):641-644.
[5]LUAN B,AKSIMENTIEV A.Electric and electrophoretic inversion of the DNA charge in multivalent electrolytes[J].Soft Matter,2010,6(2):243-246.
[6]FU W B,WANG X L,ZHANG X H,et al.Compaction dynamics of single DNA molecules under tension[J].Journal of the American Chemical Society,2006,128(47):15040-15041.
[7]任朝华,宓博凯,何俊杰,等.介电常数对三氯六氨络合钴导致DNA凝聚的影响[J].电子显微学报,2017,36(3):273-278.
[8]金仙君,林莹,宓博凯,等.乙醇对鱼精蛋白与DNA相互作用的影响[J].电子显微学报,2016,35(6):516-520.
[9]PASTRED,HAMON L,LANDOUSY F,et al.Anionic polyelectrolyte adsorption on mica mediated by multivalent cations:a solution to DNA imaging by atomic force microscopy under high ionic strengths[J].Langmuir,2006,22(15):6651-6660.
[10]BESTEMAN K,ZEVENBERGEN M A,HEERING HA,et al.Direct observation of charge inversion by multivalent ions as a universal electrostatic phenomenon[J].Physical Review Letters,2004,93(17):170802.
[11]BESTEMAN K,ZEVENBERGEN M A,LEMAY S G,et al.Charge inversion by multivalent ions:dependence on dielectric constant and surface-charge density[J].Physical Review E,2005,72(6):061501.
[12]BESTEMAN K,VAN EIJK K,LEMAY S G,et al.Charge inversion accompanies DNA condensation by multivalent ions[J].Nature Physics,2007,3(9):641-644.
[2]LASIC D D.Novel applications of liposomes[J].Trends in Biotechnology,1998,16(7):307-321.
[3]ZHOU J,KE F,LIANG D.Kinetic study on the reentrant condensation of oligonucleotide in trivalent salt solution[J].The Journal of Physical Chemistry B,2010,114(43):13675-13680.
[4]BESTEMAN K,VAN EIJK K,LEMAY S G.Charge inversion accompanies DNA condensation by multivalent ions[J].Nature Physics,2007,3(9):641-644.
[5]LUAN B,AKSIMENTIEV A.Electric and electrophoretic inversion of the DNA charge in multivalent electrolytes[J].Soft Matter,2010,6(2):243-246.
[6]FU W B,WANG X L,ZHANG X H,et al.Compaction dynamics of single DNA molecules under tension[J].Journal of the American Chemical Society,2006,128(47):15040-15041.
[7]任朝华,宓博凯,何俊杰,等.介电常数对三氯六氨络合钴导致DNA凝聚的影响[J].电子显微学报,2017,36(3):273-278.
[8]金仙君,林莹,宓博凯,等.乙醇对鱼精蛋白与DNA相互作用的影响[J].电子显微学报,2016,35(6):516-520.
[9]PASTRED,HAMON L,LANDOUSY F,et al.Anionic polyelectrolyte adsorption on mica mediated by multivalent cations:a solution to DNA imaging by atomic force microscopy under high ionic strengths[J].Langmuir,2006,22(15):6651-6660.
[10]BESTEMAN K,ZEVENBERGEN M A,HEERING HA,et al.Direct observation of charge inversion by multivalent ions as a universal electrostatic phenomenon[J].Physical Review Letters,2004,93(17):170802.
[11]BESTEMAN K,ZEVENBERGEN M A,LEMAY S G,et al.Charge inversion by multivalent ions:dependence on dielectric constant and surface-charge density[J].Physical Review E,2005,72(6):061501.
[12]BESTEMAN K,VAN EIJK K,LEMAY S G,et al.Charge inversion accompanies DNA condensation by multivalent ions[J].Nature Physics,2007,3(9):641-644.