上海市某马场马疱疹病毒1型感染的确诊
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摘要
采用马疱疹病毒1型和4型二重PCR分型方法,对上海市某马场临床疑似病例的鼻拭子和血浆样品进行检测,同时对扩增产物进行测序和比对,确诊为马疱疹病毒1型阳性,表明上海市马群中存在马疱疹病毒1型感染。因此,应提高上海市马匹饲养管理水平,加强马疱疹病毒1型的定期免疫和检疫,及时对阳性马匹进行隔离,对马厩进行消毒。
In this study,the nasal swabs and plasma samples collected from suspected clinical cases in a horse farm in Shanghai City were detected by duplex-PCR assay for equine herpesvirus type 1(EHV-1)and type 4(EHV-4),meantime,the amplified products were sequenced and compared,and the EHV-1 was finally confirmed,indicating that EHV-1 infection existed in horse farms in Shanghai City. As a response,it was necessary to improve the feeding management of the farm,strengthen regular immunization and quarantine against EHV-1 and to separate and disinfect the positive horses in time.
In this study,the nasal swabs and plasma samples collected from suspected clinical cases in a horse farm in Shanghai City were detected by duplex-PCR assay for equine herpesvirus type 1(EHV-1)and type 4(EHV-4),meantime,the amplified products were sequenced and compared,and the EHV-1 was finally confirmed,indicating that EHV-1 infection existed in horse farms in Shanghai City. As a response,it was necessary to improve the feeding management of the farm,strengthen regular immunization and quarantine against EHV-1 and to separate and disinfect the positive horses in time.
引文
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[12]杨永龙,刘建华,宋焕堂,等.新疆伊犁地区马疱疹病毒1型的分离与鉴定[J].中国预防兽医学报,2016,38(7):550-553.
[2]易新萍,单文鲁,冉多良,等.应用一步PCR法检测并鉴定马疱疹病毒1型和4型[J].病毒学报,2001,17(2):158-160.
[3]单文鲁,杜建,晁群芳,等.野马鼻肺炎病毒的分离鉴定[J].中国兽医杂志,1999,25(6):3-7.
[4]田志革,侯玉杰,郭巍,等.马鼻肺炎病毒双重PCR分型检测方法的建立[J].中国兽医科学,2011,41(9):907-910.
[5]杜建,王正党.新疆普氏野马鼻肺炎病毒的分离鉴定[J].云南大学学报(自然科学版),1999,21(3):182-185.
[6]王征,郭巍,姬媛媛,等.马疱疹病毒1型SYBRGreenⅠ荧光定量PCR检测方法的建立[J].中国预防兽医学报,2012,34(2):116-119.
[7]杨夷平,刘建华,冉多良,等.马疱疹病毒1型LAMP检测方法的建立[J].中国畜牧兽医,2014,41(9):35-40.
[8]易新萍,单文鲁.套式PCR分型检测马鼻肺炎病毒的方法研究[J].中国预防兽医学报,2000,22(增刊):118-122.
[9]胡月,刘建华,鲍子磊,等.马疱疹病毒1型XJ2015株全基因组测序及分析[J].动物医学进展,2018,39(11):41-47.
[10]SOBOLL H G,ASHTON L V,QUINTANA A M,et al.Innate immune responses of airway epithelial cells to infection with equine herpesvirus-1[J].Veterinary microbiology,2014,170:28-38.
[11]MAHMOUD H Y,ANDOH K,HATTORI S,et al.Characterization of glycoproteins in equine herpesvirus-1[J].Journal of veterinary medical science,2013,75(10):1317-1321.
[12]杨永龙,刘建华,宋焕堂,等.新疆伊犁地区马疱疹病毒1型的分离与鉴定[J].中国预防兽医学报,2016,38(7):550-553.