石英玻璃微通道样品池的水质油类污染物荧光检测
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摘要
为实现水质油类污染物的准确检测,保证获得的荧光强度信号的可靠性,研制具有荧光光谱信号辅助参考功能的样品池有实际意义。首先设计石英玻璃多微通道样品池,并使用飞秒激光烧蚀石英玻璃内部的微通道结构。其次,构建荧光光路系统,采用脉冲氙灯光源作为激发光,对0#柴油、95#汽油和煤油进行荧光激发。最后,对4个微通道样品池中的样品进行荧光光谱检测,以其中1个通道的样品作为检测样品,其余3个样品池中的样品荧光强度的平均值作为辅助校正样品,当两种荧光光谱强度变化过程相近时,表明被测样品的荧光强度信号是可靠的。
In order to realize the accurate detection for oil pollutants in water and ensure the reliability of obtained fluorescence intensity signals,it is of practical significance to develop a sample pool with auxiliary reference function for fluorescence spectrum signals. Firstly,a multi-microchannel sample cell of quartz glass was designed and the microchannel structure inside quartz glass was ablated by femtosecond laser. Secondly,a fluorescent light path system is constructed,and pulsed xenon light source was used as excitation light to excite 0 # diesel oil,95 # gasoline and kerosene fluorescence. Finally,the fluorescence spectroscopy of samples in four microchannel sample pools were detected. The fluorescence intensity of the samples in one channel was used as the test sample,and the average fluorescence intensity of samples in other three sample pools was used as the auxiliary calibration sample. If the two fluorescence spectral intensities change approximately,the results show that the fluorescence intensity signal of the tested sample is reliable
In order to realize the accurate detection for oil pollutants in water and ensure the reliability of obtained fluorescence intensity signals,it is of practical significance to develop a sample pool with auxiliary reference function for fluorescence spectrum signals. Firstly,a multi-microchannel sample cell of quartz glass was designed and the microchannel structure inside quartz glass was ablated by femtosecond laser. Secondly,a fluorescent light path system is constructed,and pulsed xenon light source was used as excitation light to excite 0 # diesel oil,95 # gasoline and kerosene fluorescence. Finally,the fluorescence spectroscopy of samples in four microchannel sample pools were detected. The fluorescence intensity of the samples in one channel was used as the test sample,and the average fluorescence intensity of samples in other three sample pools was used as the auxiliary calibration sample. If the two fluorescence spectral intensities change approximately,the results show that the fluorescence intensity signal of the tested sample is reliable
引文
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[3] ZHOU Z Z,GUO L D,SHILLER A M,et al. Characterization of oil components from the Deepwater Horizon oil spill in the Gulf of Mexico using fluorescence EEM and PARAFAC techniques[J]. Marine Chemistry,2013,148.
[4] DRISKILL A K,ALVEY J,DOTSON A D,et al. Monitoring polycyclic aromatic hydrocarbon(PAH)attenuation in Arctic waters using fluorescence spectroscopy[J]. Cold Regions Science and Technology,2018,145.
[5]袁书萍.大数据背景下的激光荧光光谱数据模式识别研究[J].激光杂志,2018,39(05):124-127.
[6]陈雨卉,席宏波,于东,等.典型除草剂生产废水的有机污染特性研究[J].光谱学与光谱分析,2015,35(12):3444-3449.
[7]褚东志,马海宽,吴宁,等.水质油污染原位传感器稳定性设计[J].传感技术学报,2017,30(11):1660-1665.
[8] CARULLO P,CHINO M,CETRANGOLO G P,et al. Direct detection of organophosphate compounds in water by a fluorescence-based biosensing device[J]. Sensors&Actuators:B. Chemical,2018,255.
[9] SCHIRMER Cr,POSSECKARDT J,KICK A,et al. Encapsulating genetically modified Saccharomyces cerevisiae cells in a flow-through device towards the detection of diclofenac in wastewater[J]. Journal of Biotechnology,2018.
[10]王成.基于荧光机理的水中矿物油识别与分析系统研究[D].秦皇岛:燕山大学,2016.
[11] HALSEY C M,BENHAM D A,JIJI R D,et al. Influence of the lipid environment on valinomycin structure and cation complex formation[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2012,96.
[12] PERSICHETTI G,GRIMALDI I A,TESTA G,et al. Selfassembling and packaging of microbottle resonators for allpolymer lab-on-chip platform[J]. Sensors&Actuators:A.Physical,2018,280.
[13]许飞.散射和荧光法油田注水在线检测系统研究[D].天津:天津大学,2016.
[14]陈烽,杨青,边浩,等.飞秒激光湿法刻蚀微纳制造[J].应用光学,2014,35(01):150-154.
[15]梁向晖,毛秋平,钟伟强.日立F-7000荧光分光光度计的使用[J].分析仪器,2018(04):164-169.
[16]陈至坤,张菡洁,王玉田,等.基于小波变换的矿物油荧光光谱数据处理方法[J].激光杂志,2016,37(10):78-81.