荭草花提取物对心肌缺血再灌注损伤模型大鼠的保护作用研究
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摘要
目的:研究荭草花提取物对心肌缺血再灌注损伤(MIRI)模型大鼠的保护作用,为其药用资源的深度开发提参考。方法:将24只大鼠随机分为假手术组(生理盐水)、模型组(生理盐水)、复方丹参片组(阳性对照,0.17 g/kg)和荭草花提取物组(以生药计为86 g/kg),每组6只。每天灌胃给药1次,剂量均为2 m L/100 g。连续给药4 d后,除假手组外,其余各组大鼠均采用左冠状动脉前降支法复制MIRI模型。再灌注24 h后再给药1次,给药结束后监测各组大鼠心电图ST段变化,检测各组大鼠血浆中乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)、肌酸激酶(CK)、心肌钙蛋白(cTn-I)、超氧化物歧化酶(SOD)和一氧化氮(NO)水平,计算各组大鼠心肌梗死率并观察其心肌组织病理形态学变化。结果:与假手术组比较,模型组大鼠心电图ST段显著抬高(P<0.01);血浆中LDH、CK、CK-MB、cTn-I水平显著升高(P<0.01),SOD、NO水平显著降低(P<0.01);心肌梗死率显著升高(P<0.01),心肌组织发生炎性细胞浸润、心肌细胞胞质结构疏松等病理形态学改变。与模型组比较,复方丹参片组和荭草花提取物组大鼠心电图ST段显著下移(P<0.05);血浆中LDH、CK、CK-MB、cTn-I水平显著降低(P<0.05),SOD、NO水平显著升高(P<0.05);心肌梗死率显著降低(P<0.05),心肌组织中炎性细胞浸润、组织水肿等病变不同程度减轻。结论:荭草花提取物可能通过抗氧化损伤发挥其对MIRI的保护作用。
OBJECTIVE:To study the protective effects of Polygonum orientale extract on myocardial ischemia-reperfusion injury(MIRI)model rats,and to provide reference for it's deeply development of medicinal source. METHODS:Totally 24 rats were randomly divided into sham operation group(normal saline),model group(normal saline),Compound danshen tablet group(positive group,0.17 g/kg)and P. orientale extract group(86 g/kg,calculated by crude drug),with 6 rats in each group. All groups were given drugs 2 m L/100 g intragastrically once a day. After 4 d of consecutive administration,MIRI model was induced by the left anterior descending branch of arteria coronaria in all groups except for sham operation group. 24 h after reperfusion,they were given related medicine again. After medication,the changes of electrocardiogram ST segment were monitored in each group. The plasma levels of LDH,CK-MB,CK,cTn-I,SOD and NO were detected in each group. The myocardial infarction rate in each group was calculated and the pathomorphological changes in the myocardium were observed. RESULTS:Compared with sham operation group,ST segment of myocardial electrocardiogram was increased in model group(P<0.01). The plasma levels of LDH,CK,CK-MB and cTn-I were increased significantly(P<0.01),while the plasma levels of SOD and NO were decreased significantly(P<0.01). The rate of myocardial infarction was increased significantly(P<0.01),and pathomorphological changes were observed in myocardial tissue such as infiltration of inflammatory cells and loose cytoplasm of cardiac myocytes. Compared with model group,ST segment of myocardial electrocardiogram was decreased significantly in Compound danshen tablet group and P. orientale extract group(P<0.05);the plasma levels of LDH,CK,CK-MB and c Tn-I were decreased significantly(P<0.05),while the plasma levels of SOD and NO were increased significantly(P<0.05);the rate of myocardial infarction was decreased significantly(P<0.05),and inflammatory cell infiltration and tissue edema in myocardium were relieved to varying degrees.CONCLUSIONS:The protective effect of P. orientale extract protect on MIRI may be exerted by anti-oxidative damage.
OBJECTIVE:To study the protective effects of Polygonum orientale extract on myocardial ischemia-reperfusion injury(MIRI)model rats,and to provide reference for it's deeply development of medicinal source. METHODS:Totally 24 rats were randomly divided into sham operation group(normal saline),model group(normal saline),Compound danshen tablet group(positive group,0.17 g/kg)and P. orientale extract group(86 g/kg,calculated by crude drug),with 6 rats in each group. All groups were given drugs 2 m L/100 g intragastrically once a day. After 4 d of consecutive administration,MIRI model was induced by the left anterior descending branch of arteria coronaria in all groups except for sham operation group. 24 h after reperfusion,they were given related medicine again. After medication,the changes of electrocardiogram ST segment were monitored in each group. The plasma levels of LDH,CK-MB,CK,cTn-I,SOD and NO were detected in each group. The myocardial infarction rate in each group was calculated and the pathomorphological changes in the myocardium were observed. RESULTS:Compared with sham operation group,ST segment of myocardial electrocardiogram was increased in model group(P<0.01). The plasma levels of LDH,CK,CK-MB and cTn-I were increased significantly(P<0.01),while the plasma levels of SOD and NO were decreased significantly(P<0.01). The rate of myocardial infarction was increased significantly(P<0.01),and pathomorphological changes were observed in myocardial tissue such as infiltration of inflammatory cells and loose cytoplasm of cardiac myocytes. Compared with model group,ST segment of myocardial electrocardiogram was decreased significantly in Compound danshen tablet group and P. orientale extract group(P<0.05);the plasma levels of LDH,CK,CK-MB and c Tn-I were decreased significantly(P<0.05),while the plasma levels of SOD and NO were increased significantly(P<0.05);the rate of myocardial infarction was decreased significantly(P<0.05),and inflammatory cell infiltration and tissue edema in myocardium were relieved to varying degrees.CONCLUSIONS:The protective effect of P. orientale extract protect on MIRI may be exerted by anti-oxidative damage.
引文
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[14]熊佳,周惠芬,何昱.银杏达莫注射液对缺氧缺糖损伤大鼠心肌细胞的保护作用[J].中国药学杂志,2018,53(6):421-426.
[15]DONG RL,XU XZ,LI G,et al.Bradykinin inhibits oxidative stress-induced cardiomyocytes senescence via regulating redox state[J].PLoS One,2013,8(10):e77034.
[16]黄勇,郑林,王爱民,等.荭草不同药用部位抗心肌缺血作用及化学成分比较研究[J].时珍国医国药,2010,21(10):2520-2522.
[17]李勇军,何迅,刘志宝,等.荭草花水溶性化学成分的研究[J].时珍国医国药,2010,21(1):14-15.
[18]向文英,梅朝叶,孙慧园,等.基于UHPLC-Q-TOF-MS分析荭草花提取物在H9c2心肌细胞中效应物质[J].中国实验方剂学杂志,2017,23(12):190-195.
[2]王爱民,鄢艳,郑林,等.UPLC同时测定荭草花中7种指标成分的含量[J].中国中药杂志,2011,36(22):3141-3145.
[3]张宇瑶,佟苗苗,初正云,等.荭草花HPLC特征指纹图谱研究[J].中药材,2013,36(12):1937-1939.
[4]郑林,陈慧,王爱民,等.超高效液相色谱测定大鼠口服荭草花后入血成分[J].中国医院药学杂志,2012,32(2):91-93.
[5]黄勇,何峰,郑林,等.水荭花药材中8个指标成分的UPLC-MS/MS法测定[J].中国医药工业杂志,2012,43(8):693-696.
[6]李勇军,何迅,刘志宝,等.荭草花化学成分的研究[J].中国中药杂志,2009,34(20):2613-2615.
[7]李月婷,胡杰,谢玉敏,等.荭草花不同制备工艺样品对犬急性心肌缺血的保护作用[J].中药材,2015,38(1):115-118.
[8]杨华,董兵,宋莹莹.葛根素配伍丹酚酸B注射使用对大鼠心肌缺血再灌注损伤的保护作用[J].中国药房,2017,28(25):3525-3527.
[9]李晓民,汪克明,刘婧,等.大鼠心肌缺血再灌注模型的有效复制方法[J].中国心血管病研究杂志,2007,5(6):463-464.
[10]马雯奂,韩丹,方伟蓉,等.一氧化氮在心肌缺血再灌注损伤中的调节作用及相关治疗药物研究进展[J].药学进展,2016,40(2):96-103.
[11]HOU JY,WANG XH,LI H,et al.Protective effect of GSTT on myocardium injuried by ischemia reperfusion[J].Chin Pharmacol Bull,2004,20(4):418-420.
[12]MOENS AL,CLAEYS MJ,TIMMERMANS JP,et al.Myocardialischemia/reperfusion-injury,a clinical view on a complex patho-physiological process[J].Int J Cardio,2005,100(2):179-190.
[13]许波华,许立.中药抗心肌缺血作用机制的研究进展[J].中国实验方剂学杂志,2011,17(15):265-269.
[14]熊佳,周惠芬,何昱.银杏达莫注射液对缺氧缺糖损伤大鼠心肌细胞的保护作用[J].中国药学杂志,2018,53(6):421-426.
[15]DONG RL,XU XZ,LI G,et al.Bradykinin inhibits oxidative stress-induced cardiomyocytes senescence via regulating redox state[J].PLoS One,2013,8(10):e77034.
[16]黄勇,郑林,王爱民,等.荭草不同药用部位抗心肌缺血作用及化学成分比较研究[J].时珍国医国药,2010,21(10):2520-2522.
[17]李勇军,何迅,刘志宝,等.荭草花水溶性化学成分的研究[J].时珍国医国药,2010,21(1):14-15.
[18]向文英,梅朝叶,孙慧园,等.基于UHPLC-Q-TOF-MS分析荭草花提取物在H9c2心肌细胞中效应物质[J].中国实验方剂学杂志,2017,23(12):190-195.