有髯鸢尾种质离体保存研究
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摘要
以有髯鸢尾1A(印度首领×白与蓝)继代5次组培苗为试验材料,以MS+2. 0 mg/L 6-BA+0. 5 mg/L IBA为基本培养基,分别添加不同质量浓度的多效唑(PP_(333))、比久(B9)、矮壮素(CCC)、脱落酸(ABA)、甘露醇、山梨醇,采用单因素试验设计,研究不同药剂及其质量浓度对组培苗离体保存的影响,并应用隶属函数法对各药剂浓度的保存效果进行综合评价。结果表明,6种药剂对有髯鸢尾1A组培苗的生长有较强的抑制作用。同一药剂不同浓度间指标的差异不同,分别添加PP_(333)、B9、甘露醇、山梨醇时,不同质量浓度处理的组培苗存活率均为100%;分别添加CCC、ABA时,不同质量浓度的组培苗存活率差异明显。当PP_(333)为8. 0 mg/L及16. 0 mg/L、CCC为20. 0 mg/L及40. 0 mg/L、甘露醇为30. 0 g/L及40. 0 g/L、山梨醇为30. 0 g/L时,株丛黄叶率均为0;当CCC为5. 0 mg/L时,株丛增殖倍数最高,为5. 8倍;当PP_(333)为1. 0 mg/L时,株高最大,为10. 5 cm。综合考虑,PP_(333)、B9、CCC、ABA和甘露醇、山梨醇的最适质量浓度分别为8. 0、15. 0、40. 0、4. 0 mg/L和40. 0、30. 0 g/L。隶属函数法综合评价结果表明,有髯鸢尾1A组培苗种质离体保存的最佳处理为甘露醇40. 0 g/L,培养至120 d时,其株丛存活率为100%,无黄叶现象,增殖倍数为2. 9,株高为2. 6 cm。
Bearded Iris 1A( reciprocal cross offspring of Iris germanica Indian Chief and Iris germanica Baiyulan) were used as experimental materials. Using single factor design method,the effects of PP_(333),B9,CCC,ABA,mannitol and sorbitol on preservation in vitro were studied. The results showed that,the growth of tissue culture seedlings was greatly inhibited by 6 growth regulators. The difference of indicators between different concentrations was different. When PP_(333),B9,mannitol and sorbitol were added respectively,the survival rates of tissue culture seedlings reached 100%,When CCC and ABA were added respectively,the survival rates of tissue culture seedlings were obviously different. When the concentration of PP_(333) was 8. 0 mg/L and 16. 0 mg/L,CCC was 20. 0 mg/L and 40. 0 mg/L,mannitol was 30. 0 g/L and40. 0 g/L,sorbitol was 30. 0 g/L,the rate of yellow leaves was 0. When the concentration of CCC was5. 0 mg/L,the multiplication multiple was the highest(5. 8 times);When the concentration of PP_(333)was1. 0 mg/L,the plant height was the highest(10. 5 cm). Comprehensive consideration showed that the optimum treatment of PP_(333),B9,CCC,ABA,mannitol and sorbitol were 8. 0 mg/L,15. 0 mg/L,40. 0 mg/L,4. 0 mg/L,40. 0 g/L and 30. 0 g/L,respectively. The comprehensive evaluation results of membership function method showed that the optimum treatment of 1A was mannitol with the concentration of40. 0 g/L. When cultured to 120 d,the survival rate was 100%,and there was no yellow leaf,the multiplication rate was 2. 9,and the plant height was 2. 6 cm.
Bearded Iris 1A( reciprocal cross offspring of Iris germanica Indian Chief and Iris germanica Baiyulan) were used as experimental materials. Using single factor design method,the effects of PP_(333),B9,CCC,ABA,mannitol and sorbitol on preservation in vitro were studied. The results showed that,the growth of tissue culture seedlings was greatly inhibited by 6 growth regulators. The difference of indicators between different concentrations was different. When PP_(333),B9,mannitol and sorbitol were added respectively,the survival rates of tissue culture seedlings reached 100%,When CCC and ABA were added respectively,the survival rates of tissue culture seedlings were obviously different. When the concentration of PP_(333) was 8. 0 mg/L and 16. 0 mg/L,CCC was 20. 0 mg/L and 40. 0 mg/L,mannitol was 30. 0 g/L and40. 0 g/L,sorbitol was 30. 0 g/L,the rate of yellow leaves was 0. When the concentration of CCC was5. 0 mg/L,the multiplication multiple was the highest(5. 8 times);When the concentration of PP_(333)was1. 0 mg/L,the plant height was the highest(10. 5 cm). Comprehensive consideration showed that the optimum treatment of PP_(333),B9,CCC,ABA,mannitol and sorbitol were 8. 0 mg/L,15. 0 mg/L,40. 0 mg/L,4. 0 mg/L,40. 0 g/L and 30. 0 g/L,respectively. The comprehensive evaluation results of membership function method showed that the optimum treatment of 1A was mannitol with the concentration of40. 0 g/L. When cultured to 120 d,the survival rate was 100%,and there was no yellow leaf,the multiplication rate was 2. 9,and the plant height was 2. 6 cm.
引文
[1]张巧仙,范冬梅.植物种质资源的保存——离体保存[J].太原师范学院学报(自然科学版),2005,4(1):82-85.
[2]叶秀仙,黄敏玲,吴建设,等.甘露醇和生长抑制剂对文心兰离体保存的影响[J].福建农业学报,2011,26(1):76-82.
[3]张晓丽,刘文英,张楠,等. PP333对怀地黄种质离体保存的影响[J].河南师范大学学报(自然科学版),2009,37(3):171-174.
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[5]朱云芬,程群,李卫东,等.植物生长延缓剂B9对马铃薯种质资源离体保存的影响[J].湖北农业科学,2014,53(15):3501-3503.
[6]尹明华,聂凤琴,邓小艳,等. PP333、B9和ABA对黄独种质离体保存的影响[J].黑龙江农业科学,2011(7):20-21.
[7]赵密珍,刁曼妮,钱亚明,等.多效唑对草莓种质离体保存的影响[J].植物遗传资源学报,2003,4(3):242-244.
[8]蔡鹏,吴婵榕,刘华英,等. ABA与CCC对南天竹离体保存的影响[J].中国现代中药,2016,18(9):1180-1184.
[9]田奥磊,高俊杰,李丹丹,等.茶树离体培养类型及其应用研究进展[J].河南农业科学,2017,46(5):1-7.
[10]刘燕.园林花卉学[M]. 2版.北京:中国林业出版社,2009.
[11]王清萍,张志国,贺坤.高型有髯鸢尾品种综合评价[J].北方园艺,2006(6):109-111.
[12]郭翎.中国名花丛书——鸢尾[M].上海:上海科学技术出版社,2000.
[13]黄苏珍,韩玉林,谢明云,等.中国鸢尾属观赏植物资源的研究与利用[J].中国野生植物资源,2003,22(1):4-7.
[14]黄苏珍,韩玉林,谢明云,等.杂种鸢尾的组织培养和植株再生[J].植物生理学报,2003,39(6):638.
[15]黄苏珍,居丽.荷兰鸢尾的组织培养[J].植物资源与环境学报,1999,8(3):48-52.
[16]韩宏伟,廖晴,玛尔哈巴·吾斯满,等.德国鸢尾组培快繁过程中内生菌的分离、鉴定及控制药剂筛选[J].新疆农业科学,2017,54(4):652-659.
[17]傅家瑞.种子生理[M].北京:科学出版社,1985.
[18]辛淑英,谢欣.甘露醇浓度对百合种质离体保存的影响[J].中国种业,1995(3):50-52.
[19]郭延平,李嘉瑞. ABA对猕猴桃种质离体保存的生理效应[J].西北农业学报,1995,4(1):84-87.
[20]张敏,王明梅,王晨阳,等. 5种常绿阔叶植物在北京越冬的抗寒性研究[J].河南农业科学,2017,46(10):116-121.
[21]胡妍妍,李钊,骆建霞.干旱胁迫对海棠幼苗生长及生理特性的影响[J].河南农业科学,2017,46(6):104-107.
[22]张乐华,王书胜,单文,等.基质、激素种类及其浓度对鹿角杜鹃扦插育苗的影响[J].林业科学,2014,50(3):45-54.
[23]赵卫星,常高正,高宁宁,等.甜瓜种质资源芽苗期和幼苗期耐NO-3性评价及鉴定指标筛选[J].河南农业科学,2018,47(1):84-89.
[24]洪森荣,尹明华. 5种植物生长抑制剂对香果树种质离体保存的影响[J].亚热带植物科学,2009,38(4):18-21.
[25]徐志微,杨丽丽,杜国强,等.培养基渗透压和生长调节剂对葡萄种质离体保存的效应[J].分子植物育种,2014,12(4):720-725.
[2]叶秀仙,黄敏玲,吴建设,等.甘露醇和生长抑制剂对文心兰离体保存的影响[J].福建农业学报,2011,26(1):76-82.
[3]张晓丽,刘文英,张楠,等. PP333对怀地黄种质离体保存的影响[J].河南师范大学学报(自然科学版),2009,37(3):171-174.
[4]辛淑英,谢欣.甘露醇浓度对百合种质离体保存的影响[J].中国种业,1995(3):50-52.
[5]朱云芬,程群,李卫东,等.植物生长延缓剂B9对马铃薯种质资源离体保存的影响[J].湖北农业科学,2014,53(15):3501-3503.
[6]尹明华,聂凤琴,邓小艳,等. PP333、B9和ABA对黄独种质离体保存的影响[J].黑龙江农业科学,2011(7):20-21.
[7]赵密珍,刁曼妮,钱亚明,等.多效唑对草莓种质离体保存的影响[J].植物遗传资源学报,2003,4(3):242-244.
[8]蔡鹏,吴婵榕,刘华英,等. ABA与CCC对南天竹离体保存的影响[J].中国现代中药,2016,18(9):1180-1184.
[9]田奥磊,高俊杰,李丹丹,等.茶树离体培养类型及其应用研究进展[J].河南农业科学,2017,46(5):1-7.
[10]刘燕.园林花卉学[M]. 2版.北京:中国林业出版社,2009.
[11]王清萍,张志国,贺坤.高型有髯鸢尾品种综合评价[J].北方园艺,2006(6):109-111.
[12]郭翎.中国名花丛书——鸢尾[M].上海:上海科学技术出版社,2000.
[13]黄苏珍,韩玉林,谢明云,等.中国鸢尾属观赏植物资源的研究与利用[J].中国野生植物资源,2003,22(1):4-7.
[14]黄苏珍,韩玉林,谢明云,等.杂种鸢尾的组织培养和植株再生[J].植物生理学报,2003,39(6):638.
[15]黄苏珍,居丽.荷兰鸢尾的组织培养[J].植物资源与环境学报,1999,8(3):48-52.
[16]韩宏伟,廖晴,玛尔哈巴·吾斯满,等.德国鸢尾组培快繁过程中内生菌的分离、鉴定及控制药剂筛选[J].新疆农业科学,2017,54(4):652-659.
[17]傅家瑞.种子生理[M].北京:科学出版社,1985.
[18]辛淑英,谢欣.甘露醇浓度对百合种质离体保存的影响[J].中国种业,1995(3):50-52.
[19]郭延平,李嘉瑞. ABA对猕猴桃种质离体保存的生理效应[J].西北农业学报,1995,4(1):84-87.
[20]张敏,王明梅,王晨阳,等. 5种常绿阔叶植物在北京越冬的抗寒性研究[J].河南农业科学,2017,46(10):116-121.
[21]胡妍妍,李钊,骆建霞.干旱胁迫对海棠幼苗生长及生理特性的影响[J].河南农业科学,2017,46(6):104-107.
[22]张乐华,王书胜,单文,等.基质、激素种类及其浓度对鹿角杜鹃扦插育苗的影响[J].林业科学,2014,50(3):45-54.
[23]赵卫星,常高正,高宁宁,等.甜瓜种质资源芽苗期和幼苗期耐NO-3性评价及鉴定指标筛选[J].河南农业科学,2018,47(1):84-89.
[24]洪森荣,尹明华. 5种植物生长抑制剂对香果树种质离体保存的影响[J].亚热带植物科学,2009,38(4):18-21.
[25]徐志微,杨丽丽,杜国强,等.培养基渗透压和生长调节剂对葡萄种质离体保存的效应[J].分子植物育种,2014,12(4):720-725.