盐胁迫对4个水稻种质抗逆性生理的影响
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摘要
为筛选水稻(Oryza sativa)耐盐种质资源,研究了水稻耐盐相关生理指标。结果表明,盐胁迫下,耐盐种质Pokkali、Fl478、JX99的电导率较低,细胞膜透性较小,叶片叶绿素含量较高,受盐害程度较弱。盐胁迫促进了丙二醛的大量积累,加剧了细胞膜脂过氧化程度,而耐盐种质的丙二醛积累较少,但高盐胁迫下3个耐盐种质的丙二醛含量升高,破坏了细胞膜的完整性。感盐种质R29的临界饱和亏的增幅较大,耐盐种质相对含水量降幅小。应用模糊隶属函数法综合评价,4个水稻种质的耐盐性依次为JX99>Pokkali>FL478>IR29。
In order to select salt-tolerance germplasms of rice(Oryza sativa) germplasms, the physiological indexes related salt tolerance were studied under salt stress. The results showed that the salt tolerance germplasms,such as Pokkali, Fl478 and JX99, had low conductivity, small cell membrane permeability, and high chlorophyll content in leaf, showing weak salt damage. The malondialdehyde content, the degree of cell membrane lipid peroxidation increased under salt stress. The malondialdehyde accumulated in salt-tolerant germplasm less than that in sensitive germplasm, but the malondialdehyde content in three salt-tolerant germplasms also increased under high salt stress, which destroyed the integrity of cell membrane. The critical saturation deficit in sensitive germplasm R29 greatly enhanced, while the relative water content in salt-tolerant germplasms slightly decreased.So, the order of salt tolerance was JX99>Pokkali > FL478> IR29 by fuzzy membership function method.
In order to select salt-tolerance germplasms of rice(Oryza sativa) germplasms, the physiological indexes related salt tolerance were studied under salt stress. The results showed that the salt tolerance germplasms,such as Pokkali, Fl478 and JX99, had low conductivity, small cell membrane permeability, and high chlorophyll content in leaf, showing weak salt damage. The malondialdehyde content, the degree of cell membrane lipid peroxidation increased under salt stress. The malondialdehyde accumulated in salt-tolerant germplasm less than that in sensitive germplasm, but the malondialdehyde content in three salt-tolerant germplasms also increased under high salt stress, which destroyed the integrity of cell membrane. The critical saturation deficit in sensitive germplasm R29 greatly enhanced, while the relative water content in salt-tolerant germplasms slightly decreased.So, the order of salt tolerance was JX99>Pokkali > FL478> IR29 by fuzzy membership function method.
引文
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[18]ZHAO M L,DU Q L,JIAO J,et al.Physiological response and salt resistance evaluation of six varieties of Olea europaea under salt stress[J].J Fujian Agric For Univ(Nat Sci),2016,45(1):19-25.doi:10.13323/j.cnki.j.fafu(nat.sci.).2016.01.004.赵曼利,杜启兰,焦健,等.盐胁迫对不同品种油橄榄抗盐性生理指标的影响[J].福建农林大学学报(自然科学版),2016,45(1):19-25.doi:10.13323/j.cnki.j.fafu(nat.sci.).2016.01.004.
[2]LI B,WANG Z C,SUN Z G,et al.Resources and sustainable resource exploitation of salinized land in China[J].Agric Res Arid Areas,2005,23(2):154-158.doi:10.3321/j.issn:1000-7601.2005.02.032.李彬,王志春,孙志高,等.中国盐碱地资源与可持续利用研究[J].干旱地区农业研究,2005,23(2):154-158.doi:10.3321/j.issn:1000-7601.2005.02.032.
[3]ZHOU Y,CUI F L,YANG P,et al.Effect of salt stress on physiological and biochemical characteristics of different rice varieties during seedling stage[J].Jiangsu Agric Sci,2016,44(1):90-93.doi:10.15889/j.issn.1002-1302.2016.01.023.周毅,崔丰磊,杨萍,等.盐胁迫对不同品种水稻幼苗生理生化特性的影响[J].江苏农业科学,2016,44(1):90-93.doi:10.15889/j.issn.1002-1302.2016.01.023.
[4]MUNNS R,TESTER M.Mechanisms of salinity tolerance[J].Annu Rev Plant Biol,2008,59:651-681.
[5]WANG N,CAO M J,YU J L.Effects of NaCl Stress on organic osmotica of maize seedlings[J].J Maize Sci,2009,17(4):61-65,69.doi:10.13597/j.cnki.maize.science.2009.04.020.王宁,曹敏建,于佳林.NaCl胁迫对玉米幼苗有机渗透调节物质的影响[J].玉米科学,2009,17(4):61-65,69.doi:10.13597/j.cnki.maize.science.2009.04.020.
[6]XU C,LING F L,XU K Z,et al.Effect of salt stress on photosynthetic characteristics and physiological and biochemical traits of different rice varieties[J].Chin J Rice Sci,2013,27(3):280-286.doi:10.3969/j.issn.1001-7216.2013.03.008.徐晨,凌风楼,徐克章,等.盐胁迫对不同水稻品种光合特性和生理生化特性的影响[J].中国水稻科学,2013,27(3):280-286.doi:10.3969/j.issn.1001-7216.2013.03.008.
[7]ZHOU Z F,LI Z A.Guidance on Plant Physiological Experiment[M].Beijing:China Agricultural Press,2005:114-116.周祖富,黎兆安.植物生理学试验指导[M].北京:中国农业出版社,2005:114-116.
[8]ZHOU B,GUO Z,LIU Z.Effects of abscisic acid on antioxidant systems of Stylosanthes guianensis(Aublet)Sw.under chilling stress[J].Crop Sci,2005,45(2):599-605.doi:10.2135/cropsci2005.0599.
[9]XU L Z,PANG S Q,DIAO M,et al.Comprehensive evaluation of salt tolerance of different processing tomato cultivars by membership function method[J].Xinjiang Agric Sci,2017,54(5):833-842.doi:10.6048/j.issn.1001-4330.2017.05.006.许立志,庞胜群,刁明,等.隶属函数法评价不同加工番茄品种耐盐性[J].新疆农业科学,2017,54(5):833-842.doi:10.6048/j.issn.1001-4330.2017.05.006.
[10]PARDO J M.Biotechnology of water and salinity stress tolerance[J].Curr Opin Biotechnol,2010,21(2):185-196.doi:10.1016/j.copbio.2010.02.005.
[11]XIAO J,CHEN X,ZHAO Y,et al.Research overview and prospect on rice under salt stress[J].Liaoning Agric Sci,2012(6):48-53.doi:10.3969/j.issn.1002-1728.2012.06.012.肖军,陈珣,肇莹,等.水稻盐胁迫的研究概况与展望[J].辽宁农业科学,2012(6):48-53.doi:10.3969/j.issn.1002-1728.2012.06.012.
[12]YANG G H,SHI D C.Effects of Na Cl stresses on relative water contents and defense enzymes activities in leaves of Glycyrrhiza uralensis Fisch.[J].J Henan Agric Sci,2009,38(12):104-106.doi:10.3969/j.issn.1004-3268.2009.12.032.杨国会,石德成.NaCl胁迫对甘草叶片相对含水量及保护酶活性的影响[J].河南农业科学,2009,38(12):104-106.doi:10.3969/j.issn.1004-3268.2009.12.032.
[13]SHI L R,NIU Y L,LI M Z.Physiological response of Sonchus brachyotus to salt stress[J].Acta Pratacult Sin,2010,19(6):272-275.时丽冉,牛玉璐,李明哲.苣荬菜对盐胁迫的生理响应[J].草业学报,2010,19(6):272-275.
[14]YANG L F,ZHU Y L,HU C M,et al.Effects of Na Cl stress on the contents of the substances regulating membrane lipid oxidation and osmosis and photosynthetic characteristics of grafted cucumber[J].Acta Bot Boreali-Occid Sin,2006,26(6):1195-1200.doi:10.3321/j.issn:1000-4025.2006.06.017.杨立飞,朱月林,胡春梅,等.NaCl胁迫对嫁接黄瓜膜脂过氧化、渗透调节物质含量及光合特性的影响[J].西北植物学报,2006,26(6):1195-1200.doi:10.3321/j.issn:1000-4025.2006.06.017.
[15]TAN W J,LI Q Y,SHEN X F,et al.Study on seed germination of weedy rice and cultivated rice under salinity stress[J].Guangdong Agric Sci,2014(3):5-8.doi:10.3969/j.issn.1004-874X.2014.03.002.谭卫军,李其勇,沈雪峰,等.盐胁迫对杂草稻和水稻种子萌发特性的影响[J].广东农业科学,2014(3):5-8.doi:10.3969/j.issn.1004-874X.2014.03.002.
[16]KOCA H,BOR M,?ZDEMIR F,et al.The effect of salt stress on lipid peroxidation,antioxidative enzymes and proline content of sesame cultivars[J].Environ Exp Bot,2007,60(3):344-351.doi:10.1016/j.envexpbot.2006.12.005.
[17]LI Q,LIU J H,WU J Y,et al.Effect of salt stress on membrane permeability and Na+,K+absorption of oat[J].Acta Agric Boreali Sin,2009,24(6):88-92.李倩,刘景辉,武俊英,等.盐胁迫对燕麦质膜透性及Na+、K+吸收的影响[J].华北农学报,2009,24(6):88-92.
[18]ZHAO M L,DU Q L,JIAO J,et al.Physiological response and salt resistance evaluation of six varieties of Olea europaea under salt stress[J].J Fujian Agric For Univ(Nat Sci),2016,45(1):19-25.doi:10.13323/j.cnki.j.fafu(nat.sci.).2016.01.004.赵曼利,杜启兰,焦健,等.盐胁迫对不同品种油橄榄抗盐性生理指标的影响[J].福建农林大学学报(自然科学版),2016,45(1):19-25.doi:10.13323/j.cnki.j.fafu(nat.sci.).2016.01.004.