金针菇退化菌株复壮条件的优化初探
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摘要
金针菇在工厂化栽培的过程中,经常出现菌种退化问题,严重影响产量和质量。试以金针菇的退化菌株为材料,通过组织分离、菌丝尖端分离的方法进行复壮,通过接种在4种不同培养基质上进行生长速度测定,筛选最适合金针菇复壮菌株生长的基质,并考察不同光质对复壮菌株生长的影响。结果表明:构树基质可以促进金针菇复壮菌株生长;相对于白光和红光,蓝光更有利于金针菇复壮菌株的生长,并在一定程度上提高了产量。
Industrial cultivation of Flammulina filiformis strains are often affected by degeneration leading to yield and quality loss. In this study, two degenerated strains of Flammulina filiformis were used as starter materials from which rejuvenated strains were obtained by tissue and mycelial tip isolation technique. The most suitable media for reviving the growth of the degenerated strains was screened by measuring their growth rate on four different media, and the effects of different light quality on their growth were also investigated.The results showed that a media prepared from Broussonetia papvrifera in addition to blue light was the best in rejuvenating the growth of degenerated strains of Flammulina filiformis and increased their yield to a certain extent. This rejuvenation method will provide technical support for commercial production of Flammulina filiformis, as well as in the preservation of strains.
Industrial cultivation of Flammulina filiformis strains are often affected by degeneration leading to yield and quality loss. In this study, two degenerated strains of Flammulina filiformis were used as starter materials from which rejuvenated strains were obtained by tissue and mycelial tip isolation technique. The most suitable media for reviving the growth of the degenerated strains was screened by measuring their growth rate on four different media, and the effects of different light quality on their growth were also investigated.The results showed that a media prepared from Broussonetia papvrifera in addition to blue light was the best in rejuvenating the growth of degenerated strains of Flammulina filiformis and increased their yield to a certain extent. This rejuvenation method will provide technical support for commercial production of Flammulina filiformis, as well as in the preservation of strains.
引文
[1]Wang PM,Liu XB,Dai YC,et al.Phylogeny and species delimitation of Flammulina:taxonomic status of winter mushroom in East Asia and a new European species identified using an integrated approach[J].Mycological Progress,2018(7):1-18.
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[3]钱磊,张志军,周永斌,等.光对食用菌生长的影响[J].天津农业科学,2017,23(7):103-106.
[4]刘明月,何长征.光质对金针菇子实体生长发育的影响[J].中国食用菌,1997(6):11-13.
[5]张杨.金针菇的光效应[J].食用菌,1984(1):29.
[6]李亚娇,郭九峰.食用菌菌种退化机制及预防措施的最新研究进展[J].黑龙江农业科学,2018(2):136-139.
[7]Magae Y,Hayashi N.Double-stranded RNA and virus-like particles in the edible basidiomycete Flammulina velutipes(Enokitake)[J].FEMS microbiology Letters,1999,180(2):331-335.
[8]Magae Y,Sunagawa M.Characterization of a mycovirus associated with the brown discoloratin of edible mushroom,Flammulina velutipes[J].Virology Journal,2010(7):342.
[9]张俊玲,章炉军,尚晓冬,等.主栽金针菇品种中病毒的快速检测和鉴定[J].中国农学通报,2015,31(6):234-240.
[10]刘昆昂,张根伟,马宏,等.黄色金针菇菌株提纯复壮技术研究[J].食用菌,2017,39(2):21-23.
[11]陈志松.工厂化栽培金针菇菌种退化与复壮的研究[J].中国食用菌,1998(05):10.
[12]周君强.食用菌菌种复壮四法[J].吉林农业,2007(03):38.
[13]张沿江,张翠艳,吴金玉.食用菌菌种复壮的方法[J].农村科学实验,2003(5):34.
[14]张俊玲,章炉军,刘建辉,等.金针菇褐化病毒(FvBV)脱毒方法[J].微生物学通报,2015,42(10):1952-1961.
[15]姚卫红,张丽娜,刘美秀.浅谈菌种的退化及有效包藏方法[J].中国卫生产业,2013(3):189-189.
[16]赫朝灿.菌种退化的原因、处理措施及菌种保藏探析[J].生物技术世界,2015(2):1.
[17]佟希丹.不同光质LED对黄白品种金针菇商品性状与产量的影响[D].长春:吉林农业大学,2012.
[2]戴玉成,杨祝良.中国五种重要食用菌学名新注[J/OL].菌物学报:1-6[2018-12-11].https://doi.org/10.13346/j.mycosystema.180117.
[3]钱磊,张志军,周永斌,等.光对食用菌生长的影响[J].天津农业科学,2017,23(7):103-106.
[4]刘明月,何长征.光质对金针菇子实体生长发育的影响[J].中国食用菌,1997(6):11-13.
[5]张杨.金针菇的光效应[J].食用菌,1984(1):29.
[6]李亚娇,郭九峰.食用菌菌种退化机制及预防措施的最新研究进展[J].黑龙江农业科学,2018(2):136-139.
[7]Magae Y,Hayashi N.Double-stranded RNA and virus-like particles in the edible basidiomycete Flammulina velutipes(Enokitake)[J].FEMS microbiology Letters,1999,180(2):331-335.
[8]Magae Y,Sunagawa M.Characterization of a mycovirus associated with the brown discoloratin of edible mushroom,Flammulina velutipes[J].Virology Journal,2010(7):342.
[9]张俊玲,章炉军,尚晓冬,等.主栽金针菇品种中病毒的快速检测和鉴定[J].中国农学通报,2015,31(6):234-240.
[10]刘昆昂,张根伟,马宏,等.黄色金针菇菌株提纯复壮技术研究[J].食用菌,2017,39(2):21-23.
[11]陈志松.工厂化栽培金针菇菌种退化与复壮的研究[J].中国食用菌,1998(05):10.
[12]周君强.食用菌菌种复壮四法[J].吉林农业,2007(03):38.
[13]张沿江,张翠艳,吴金玉.食用菌菌种复壮的方法[J].农村科学实验,2003(5):34.
[14]张俊玲,章炉军,刘建辉,等.金针菇褐化病毒(FvBV)脱毒方法[J].微生物学通报,2015,42(10):1952-1961.
[15]姚卫红,张丽娜,刘美秀.浅谈菌种的退化及有效包藏方法[J].中国卫生产业,2013(3):189-189.
[16]赫朝灿.菌种退化的原因、处理措施及菌种保藏探析[J].生物技术世界,2015(2):1.
[17]佟希丹.不同光质LED对黄白品种金针菇商品性状与产量的影响[D].长春:吉林农业大学,2012.