杞藿饮对湿热环境睡眠剥夺大鼠骨骼肌保护及超微结构观察
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摘要
目的观察杞藿饮对湿热环境下睡眠剥夺大鼠骨骼肌的保护作用及超微结构。方法选取40只雄性SD大鼠随机分为对照(BC)组、湿热环境对照(HC)组、湿热环境睡眠剥夺(HS)组、湿热环境睡眠剥夺杞藿饮(HSQ)组,每组10只。BC组大鼠常温常湿下饲养,HC组、HS组、HSQ组大鼠模拟湿热环境下饲养。BC组、HC组、HS组大鼠给予蒸馏水灌胃,HSQ组大鼠给予杞藿饮灌胃,连续15 d。HS组、HSQ组大鼠进行72 h睡眠剥夺。检测大鼠腓肠肌组织肌酸激酶(CK)、乳酸脱氢酶(LDH)、一氧化氮(NO)和诱导型一氧化氮合酶(i NOS)水平,透射电镜观察腓肠肌组织超微结构。结果 HC组、HS组、HSQ组大鼠腓肠肌CK和LDH水平均高于BC组(P <0. 05),且HS组和HSQ组高于HC组(P <0. 01)。HC组、HS组、HSQ组大鼠腓肠肌NO和i NOS均低于BC组(P <0. 05),且HS组和HSQ组低于HC组(P <0. 01); HSQ组NO和i NOS水平均高于HS组(P <0. 01)。透射电镜观察显示,HC组、HS组、HSQ组大鼠腓肠肌均出现不同程度的超微结构变化,以HS组最严重。结论杞藿饮能明显降低湿热环境睡眠剥夺所致大鼠的骨骼肌损害程度,其机理可能与改善能量代谢,调节自由基水平,提高机体抗氧化能力有关。
Objective To observe protective effect of Qihuoyin on skeletal muscle of rats with sleep deprivation under dampness-heat environment and ultrastructure. Methods A total of 40 male SD rats were randomly divided into blank control( BC) group,dampness-heat environment control group,dampness-heat environment sleep deprivation group and dampness-heat environment sleep deprivation Qihuoyin group( n = 10 in each group). BC group was fed under normal temperature and damp environment,and the other three groups were fed under simulated dampness-heat environment. BC,dampness-heat environment control and dampness-heat environment sleep deprivation groups were lavaged with distilled water,while dampness-heat environment sleep deprivation Qihuoyin group was lavaged with Qihuoyin,and the treatments were continuous for 15 d,and then dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups were deprived sleep for 72 h. Levels of creatine kinase( CK),lactate dehydrogenase( LDH),nitric oxide( NO) and inducible nitric oxide synthase( i NOS) in gastrocnemius muscle tissues of rats were detected,and ultrastructures of gastrocnemius muscle tissues were observed by transmission electron microscopy.Results CK and LDH levels in gastrocnemius muscle tissues in dampness-heat environment control,dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups were significantly higher than those in BC group( P < 0. 05),and the levels in dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups were significantly higher than those in dampness-heat environment control group( P < 0. 01). There were no significant differences in CK and LDH levels between dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups( P > 0. 05). NO and i NOS levels in gastrocnemius muscle tissues in dampness-heat environment control,dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups were significantly lower than those in BC group( P <0. 05),and the levels in dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups were significantly lower than those in dampness-heat environment control group( P < 0. 01),and NO and i NOS levels in dampness-heat environment sleep deprivation Qihuoyin group were significantly higher than those in dampness-heat environment sleep deprivation group( P < 0. 01). Transmission electron microscopy showed that ultrastructure of gastrocnemius muscle tissue had different degrees of changes in dampness-heat environment control,dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups,and the change in dampness-heat environment sleep deprivation group was the most serious. Conclusion Qihuoyin may obviously alleviate degree of skeletal muscle injury in rats with sleep deprivation under dampness-heat environment,and its mechanism may be related to improve energy metabolism,regulate free radicals level and enhance antioxidant capacity.
Objective To observe protective effect of Qihuoyin on skeletal muscle of rats with sleep deprivation under dampness-heat environment and ultrastructure. Methods A total of 40 male SD rats were randomly divided into blank control( BC) group,dampness-heat environment control group,dampness-heat environment sleep deprivation group and dampness-heat environment sleep deprivation Qihuoyin group( n = 10 in each group). BC group was fed under normal temperature and damp environment,and the other three groups were fed under simulated dampness-heat environment. BC,dampness-heat environment control and dampness-heat environment sleep deprivation groups were lavaged with distilled water,while dampness-heat environment sleep deprivation Qihuoyin group was lavaged with Qihuoyin,and the treatments were continuous for 15 d,and then dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups were deprived sleep for 72 h. Levels of creatine kinase( CK),lactate dehydrogenase( LDH),nitric oxide( NO) and inducible nitric oxide synthase( i NOS) in gastrocnemius muscle tissues of rats were detected,and ultrastructures of gastrocnemius muscle tissues were observed by transmission electron microscopy.Results CK and LDH levels in gastrocnemius muscle tissues in dampness-heat environment control,dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups were significantly higher than those in BC group( P < 0. 05),and the levels in dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups were significantly higher than those in dampness-heat environment control group( P < 0. 01). There were no significant differences in CK and LDH levels between dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups( P > 0. 05). NO and i NOS levels in gastrocnemius muscle tissues in dampness-heat environment control,dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups were significantly lower than those in BC group( P <0. 05),and the levels in dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups were significantly lower than those in dampness-heat environment control group( P < 0. 01),and NO and i NOS levels in dampness-heat environment sleep deprivation Qihuoyin group were significantly higher than those in dampness-heat environment sleep deprivation group( P < 0. 01). Transmission electron microscopy showed that ultrastructure of gastrocnemius muscle tissue had different degrees of changes in dampness-heat environment control,dampness-heat environment sleep deprivation and dampness-heat environment sleep deprivation Qihuoyin groups,and the change in dampness-heat environment sleep deprivation group was the most serious. Conclusion Qihuoyin may obviously alleviate degree of skeletal muscle injury in rats with sleep deprivation under dampness-heat environment,and its mechanism may be related to improve energy metabolism,regulate free radicals level and enhance antioxidant capacity.
引文
[1]马龙飞,王辉,王乃堃,等.米氮平对睡眠剥夺小鼠学习记忆以及海马组织pro BDNF、m BDNF表达的影响[J].江苏医药,2017,43(1):1-4.
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[6]蒋超,殷智宇,徐莉春,等.睡眠剥夺对记忆功能的影响机制及药物干预研究进展[J].现代医药卫生,2012,28(1):85-86.
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[8]秦明,黄裕新,王景杰,等.高湿热刺激对大鼠下丘脑内神经元和星形胶质细胞可塑性变化的影响[J].南方医科大学学报,2008,28(3):419-422.
[9]杨宝垒.湿热环境训练提高官兵5km武装越野成绩的研究[J].军事体育进修学院学报,2011,30(1):89-91.
[10]房晓,罗琳,张雷,等.湿热环境对晕动病发病的影响[J].第二军医大学学报,2010,31(6):612-614.
[11]黄燕峰,朱达坚,鲁路.黄芪多糖对慢性疲劳小鼠骨骼肌线粒体功能的影响及作用机制[J].广东医学,2017,38(12):1789-1794.
[12]殷娟娟.补充红景天对大强度运动小鼠骨骼肌Bcl-2/Bax的影响[D].石家庄:河北师范大学,2017.
[13]葛新发,董贵俊,王宏巍,等.补充枸杞对力竭运动大鼠骨骼肌超微结构变化的影响[J].山东体育学院学报,2012,28(4):46-50.
[14]孙敏.淫羊藿对过度训练大鼠腓肠肌蛋白质组表达影响的研究[D].曲阜:曲阜师范大学,2014.
[15]刘金海,安玉香,马玲.马齿苋提取物对力竭小鼠骨骼肌乳酸性无氧代谢的干预作用[J].沈阳体育学院学报,2010,29(6):94-96.
[16]张淑萍,王亚军,胡春荣,等.刺五加提取物对睡眠剥夺小鼠抗氧化抗疲劳的研究[J].黑龙江医药科学,2015,38(6):1-3.
[17]龙碧波.绞股蓝提取物对小鼠运动能力的影响[J].中国应用生理学杂志,2010,26(3):339-340.
[18]刘元平,龙健,刘纯.药物性甲状腺功能减退伴肌酸激酶升高1例及文献复习[J].重庆医科大学学报,2016(11):1192-1194.
[19]林婧娴,韩春锡,廖建湘,等.血清转氨酶和肌酸激酶测定在诊断骨骼肌疾病中的意义[J].中国医药指南,2013,11(14):83-85.
[20]贾文斌,孙金山,徐纪平,等.氨基酸维生素对力竭游泳大鼠腓肠肌超微结构和相关酶的影响[J].实用医药杂志,2013,30(7):616-618.
[21]张彦荣,高翔,毕伟.氨基胍对大鼠骨骼肌缺血再灌注肺损伤一氧化氮合酶表达的影响[J].中国老年学,2015,35(12):3269-3271
[22]段云燕,杨雅丽,马玉德.红芪总黄酮对离心运动大鼠耐力及骨骼肌中一氧化氮和一氧化氮合酶的影响[J].甘肃中医学院学报,2016,33(1):6-9.
[23]邱霓,方伟进,李聪,等.内源性一氧化氮合酶抑制物上调4周运动大鼠骨骼肌收缩功能和线粒体生物合成[J].中国病理生理杂志,2016,32(7):1259-1265.
[24]惠华强,孙金山,徐纪平,等.氨基酸维生素对力竭游泳大鼠骨胳肌超微结构及相关酶的影响[J].海军医学杂志,2013,34(2):79-81.
[25] D'Antona G,Mascaro A,Monopoli A,et al. Nitric oxide prevents atorvastatin-induced skeletal muscle dysfunction and alterations in mice[J]. Muscle Nerve,2013,47(1):72-80.
[2]陈莉弘,黄俊山.睡眠剥夺影响学习记忆机制的研究进展[J].医学研究生学报,2015,28(10):1098-1101.
[3]吴潇男,杜军.湿热环境对机体的影响及热习服建立的研究进展[J].实用医药杂志,2015,32(9):775-780.
[4]徐纪平,刘保海,孙金山,等.杞藿饮对力竭游泳大鼠肝细胞衰老及其超微结构的影响[J].海军医学杂志,2013,34(2):76-79.
[5]李宁,汪燕,刘锡禹,等.睡眠剥夺对认知功能影响的研究进展[J].生物医学工程学杂志,2008,25(5):1197-1200.
[6]蒋超,殷智宇,徐莉春,等.睡眠剥夺对记忆功能的影响机制及药物干预研究进展[J].现代医药卫生,2012,28(1):85-86.
[7]江波,赵忠新.睡眠剥夺诱发氧化应激反应研究进展[J].第二军医大学学报,2007,28(2):201-203.
[8]秦明,黄裕新,王景杰,等.高湿热刺激对大鼠下丘脑内神经元和星形胶质细胞可塑性变化的影响[J].南方医科大学学报,2008,28(3):419-422.
[9]杨宝垒.湿热环境训练提高官兵5km武装越野成绩的研究[J].军事体育进修学院学报,2011,30(1):89-91.
[10]房晓,罗琳,张雷,等.湿热环境对晕动病发病的影响[J].第二军医大学学报,2010,31(6):612-614.
[11]黄燕峰,朱达坚,鲁路.黄芪多糖对慢性疲劳小鼠骨骼肌线粒体功能的影响及作用机制[J].广东医学,2017,38(12):1789-1794.
[12]殷娟娟.补充红景天对大强度运动小鼠骨骼肌Bcl-2/Bax的影响[D].石家庄:河北师范大学,2017.
[13]葛新发,董贵俊,王宏巍,等.补充枸杞对力竭运动大鼠骨骼肌超微结构变化的影响[J].山东体育学院学报,2012,28(4):46-50.
[14]孙敏.淫羊藿对过度训练大鼠腓肠肌蛋白质组表达影响的研究[D].曲阜:曲阜师范大学,2014.
[15]刘金海,安玉香,马玲.马齿苋提取物对力竭小鼠骨骼肌乳酸性无氧代谢的干预作用[J].沈阳体育学院学报,2010,29(6):94-96.
[16]张淑萍,王亚军,胡春荣,等.刺五加提取物对睡眠剥夺小鼠抗氧化抗疲劳的研究[J].黑龙江医药科学,2015,38(6):1-3.
[17]龙碧波.绞股蓝提取物对小鼠运动能力的影响[J].中国应用生理学杂志,2010,26(3):339-340.
[18]刘元平,龙健,刘纯.药物性甲状腺功能减退伴肌酸激酶升高1例及文献复习[J].重庆医科大学学报,2016(11):1192-1194.
[19]林婧娴,韩春锡,廖建湘,等.血清转氨酶和肌酸激酶测定在诊断骨骼肌疾病中的意义[J].中国医药指南,2013,11(14):83-85.
[20]贾文斌,孙金山,徐纪平,等.氨基酸维生素对力竭游泳大鼠腓肠肌超微结构和相关酶的影响[J].实用医药杂志,2013,30(7):616-618.
[21]张彦荣,高翔,毕伟.氨基胍对大鼠骨骼肌缺血再灌注肺损伤一氧化氮合酶表达的影响[J].中国老年学,2015,35(12):3269-3271
[22]段云燕,杨雅丽,马玉德.红芪总黄酮对离心运动大鼠耐力及骨骼肌中一氧化氮和一氧化氮合酶的影响[J].甘肃中医学院学报,2016,33(1):6-9.
[23]邱霓,方伟进,李聪,等.内源性一氧化氮合酶抑制物上调4周运动大鼠骨骼肌收缩功能和线粒体生物合成[J].中国病理生理杂志,2016,32(7):1259-1265.
[24]惠华强,孙金山,徐纪平,等.氨基酸维生素对力竭游泳大鼠骨胳肌超微结构及相关酶的影响[J].海军医学杂志,2013,34(2):79-81.
[25] D'Antona G,Mascaro A,Monopoli A,et al. Nitric oxide prevents atorvastatin-induced skeletal muscle dysfunction and alterations in mice[J]. Muscle Nerve,2013,47(1):72-80.
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