人参养荣汤对大鼠运动中枢性疲劳的影响
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摘要
【目的】观察人参养荣汤对大鼠运动中枢性疲劳的干预效应。【方法】将48只大鼠随机分为空白组、模型组、支链氨基酸组、人参养荣汤组,每组12只。采用游泳力竭方法复制运动中枢性疲劳大鼠模型。人参养荣汤组、支链氨基酸组给予相应药物灌胃,空白组、模型组给予等体积生理盐水灌胃,共给药4周。给药结束后,采用比色法测定血清乳酸、血红蛋白水平;速率法测定血清肌酸激酶水平;脲酶法测定血清尿素氮水平;高效液相色谱法检测血清支链氨基酸、游离色氨酸水平;荧光分光光度法测定血清5-羟色胺、5-羟吲哚乙酸水平。【结果】与模型组比较,人参养荣汤组大鼠力竭时间明显延长,乳酸、肌酸激酶、尿素氮、游离色氨酸、5-羟色胺、5-羟吲哚乙酸水平显著降低,血红蛋白、支链氨基酸水平及羟吲哚乙酸/5-羟色胺比值显著升高(P <0.05或P <0.01)。【结论】人参养荣汤有明显的增强运动耐力和解除疲劳作用。
Objective To observe the intervention effect of Renshen Yangrong Decoction(RYD)on exerciseinduced central fatigue in rats. Methods Forty-eight rats were randomly divided into 4 groups, namely blank group,model group,branched-chain amino acid(BCAA)group and RYD group,12 rats in each group. The exercise-induced central fatigue rat model was established by exhaustive swimming method. The BCAA group and RYD group were given intragastric administration of corresponding medicine, and the blank group and model group were intragastric administration of the same volume of normal saline,the treatment covering 4 weeks. After medication,the serum levels of lactic acid and hemoglobin were determined by colorimetric method,the serum level of creatine kinase(CK)was measured by rate method, the serum level of urea nitrogen was detected by urease method,the serum levels of BCAA and free tryptophan(f-Trp)were detected by high performance liquid chromatography(HPLC),and the serum levels of 5-hydroxytryptamine(5-HT)and 5-hydroxyindole acetic acid(5-HIAA)were detected by fluorospectrophotometer. Results Compared with the model group, the time for exhausted swimming was prolonged,and the levels of lactic acid,creatine kinase,urea nitrogen,f-Trp,5-HT and 5-HIAA were reduced,and the levels of hemoglobin and branched-chain amino acid,5-HIAA/5-HT ratio were increased in RYD group(P < 0.05 or P < 0.01). Conclusion RYD can obviously enhance exercise endurance and relieve fatigue.
Objective To observe the intervention effect of Renshen Yangrong Decoction(RYD)on exerciseinduced central fatigue in rats. Methods Forty-eight rats were randomly divided into 4 groups, namely blank group,model group,branched-chain amino acid(BCAA)group and RYD group,12 rats in each group. The exercise-induced central fatigue rat model was established by exhaustive swimming method. The BCAA group and RYD group were given intragastric administration of corresponding medicine, and the blank group and model group were intragastric administration of the same volume of normal saline,the treatment covering 4 weeks. After medication,the serum levels of lactic acid and hemoglobin were determined by colorimetric method,the serum level of creatine kinase(CK)was measured by rate method, the serum level of urea nitrogen was detected by urease method,the serum levels of BCAA and free tryptophan(f-Trp)were detected by high performance liquid chromatography(HPLC),and the serum levels of 5-hydroxytryptamine(5-HT)and 5-hydroxyindole acetic acid(5-HIAA)were detected by fluorospectrophotometer. Results Compared with the model group, the time for exhausted swimming was prolonged,and the levels of lactic acid,creatine kinase,urea nitrogen,f-Trp,5-HT and 5-HIAA were reduced,and the levels of hemoglobin and branched-chain amino acid,5-HIAA/5-HT ratio were increased in RYD group(P < 0.05 or P < 0.01). Conclusion RYD can obviously enhance exercise endurance and relieve fatigue.
引文
[1]冯烨.人参养荣汤改善化疗患者疲乏70例随机对照研究[J].中国中医基础医学杂志,2014,20(6):798.
[2]孙红,李占东,王薇,等.人参养荣汤改善化疗患者疲乏的随机对照研究[J].中国中医基础医学杂志,2010,16(2):155.
[3]耿以安,景英,王恩军,等.人参养荣汤加减治疗慢性疲劳综合征[J].中国实用医药,2015,10(15):193.
[4]陈衍智,林飞,李萍萍.人参养荣汤的急性毒性及抗疲乏抗应激的研究[J].中国实验方剂学杂志,2011,17(8):225.
[5]蔡成法,李亚.反复力竭游泳运动对小鼠前脑皮层和海马的影响[J].山东体育学院学报,2014,30(4):59
[6]郭庆军,常耀明,李金声,等.大鼠游泳运动疲劳模型力竭标准的研究[J].现代生物医学进展,2010,10(15):2856.
[7]武露凌,刘钢.关于运动性疲劳动物模型建立的综述[J].体育与科学,2007,28(3):74.
[8]田峰.对中枢与外周运动性疲劳的再认识[J].中国组织工程研究,2015,19(42):6849.
[9]李晓海.运动性疲劳产生机制的生化研究进展[J].吕梁学院学报,2017,7(6):81.
[10]杨华,葛新发.补充BCAA对长时大强度训练后运动员血象的影响[J].武汉体育学院学报,2000,34(4):35.
[11]林静.疲劳产生机理及支链氨基酸对脑内5-HT影响的实验研究[J].中国应用生理学杂志,2000,16(4):357
[12]全国体育教材委员会.运动生物化学[M].北京:人民体育出版社,1999.
[13]项汉平,梅汉超,丁宝玉,等.气功足部按摩对运动性疲劳尿素氮浓度的影响[J].武汉体育学院学报,1999,33(5):86.
[14]Kremer L,Hofman J E.Teachers’professional identity and burnout[J].Research in Education,1985,34(1):89.
[15]Brickson S.The impact of identity orientation on individual and organizational outcomes in demographically diverse settings[J].Academy of Management Review,2000,25(1):82.
[16]Davis J M,Alderson N L,Welsh R S.Serotonin and central nervous system fatigue:nutritional considerations[J].Am J Clin Nutr,2000,72(2 Suppl):573S.
[17]Chaouloff F.Effects of acute physical exercise on central serotonergic systems[J].Medicine&Science in Sports&Exercise,1997,29(1):58.
[18]Hoffmann P,Elam M,Thorén P,et al.Effects of long-lasting voluntary running on the cerebral levels of dopamine,serotonin and their metabolites in the spontaneously hypertensive rat[J].Life Sci,1994,54(13):855.
[19]许玲.支链氨基酸对大鼠运动力竭后血液中抗氧化系统的影响[J].解放军体育学院学报,2002,21(4):76.
[2]孙红,李占东,王薇,等.人参养荣汤改善化疗患者疲乏的随机对照研究[J].中国中医基础医学杂志,2010,16(2):155.
[3]耿以安,景英,王恩军,等.人参养荣汤加减治疗慢性疲劳综合征[J].中国实用医药,2015,10(15):193.
[4]陈衍智,林飞,李萍萍.人参养荣汤的急性毒性及抗疲乏抗应激的研究[J].中国实验方剂学杂志,2011,17(8):225.
[5]蔡成法,李亚.反复力竭游泳运动对小鼠前脑皮层和海马的影响[J].山东体育学院学报,2014,30(4):59
[6]郭庆军,常耀明,李金声,等.大鼠游泳运动疲劳模型力竭标准的研究[J].现代生物医学进展,2010,10(15):2856.
[7]武露凌,刘钢.关于运动性疲劳动物模型建立的综述[J].体育与科学,2007,28(3):74.
[8]田峰.对中枢与外周运动性疲劳的再认识[J].中国组织工程研究,2015,19(42):6849.
[9]李晓海.运动性疲劳产生机制的生化研究进展[J].吕梁学院学报,2017,7(6):81.
[10]杨华,葛新发.补充BCAA对长时大强度训练后运动员血象的影响[J].武汉体育学院学报,2000,34(4):35.
[11]林静.疲劳产生机理及支链氨基酸对脑内5-HT影响的实验研究[J].中国应用生理学杂志,2000,16(4):357
[12]全国体育教材委员会.运动生物化学[M].北京:人民体育出版社,1999.
[13]项汉平,梅汉超,丁宝玉,等.气功足部按摩对运动性疲劳尿素氮浓度的影响[J].武汉体育学院学报,1999,33(5):86.
[14]Kremer L,Hofman J E.Teachers’professional identity and burnout[J].Research in Education,1985,34(1):89.
[15]Brickson S.The impact of identity orientation on individual and organizational outcomes in demographically diverse settings[J].Academy of Management Review,2000,25(1):82.
[16]Davis J M,Alderson N L,Welsh R S.Serotonin and central nervous system fatigue:nutritional considerations[J].Am J Clin Nutr,2000,72(2 Suppl):573S.
[17]Chaouloff F.Effects of acute physical exercise on central serotonergic systems[J].Medicine&Science in Sports&Exercise,1997,29(1):58.
[18]Hoffmann P,Elam M,Thorén P,et al.Effects of long-lasting voluntary running on the cerebral levels of dopamine,serotonin and their metabolites in the spontaneously hypertensive rat[J].Life Sci,1994,54(13):855.
[19]许玲.支链氨基酸对大鼠运动力竭后血液中抗氧化系统的影响[J].解放军体育学院学报,2002,21(4):76.
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