电感耦合等离子体-质谱法测定吐根药材中36种金属元素
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摘要
目的建立微波消解-电感耦合等离子体-质谱(ICP-MS)法测定吐根药材中36种金属元素检测方法。方法以硝酸(HNO_3)和过氧化氢(H_2O_2)作为消解体系,采用微波消解法前处理样品,以Rh、Re作为内标,通过ICP-MS法同时测定36种金属元素的量。结果 36种测定元素的线性关系良好,相关系数(r)均>0.999 5,方法重复性RSD为1.2%～6.1%,平均回收率86.5%～107.1%,RSD 1.2%～6.1%,检出限0.001～0.968μg·L~(-1)。结论该测定方法操作简便快捷、灵敏可靠,适用于吐根药材中金属元素的测定。
Objective To establish a method to determine 36 metallic elements in Ipecacuanhae by microwave digestion-ICP-MS. Methods HNO_3-H_2O_2 was used as the digestion system to treated samples by microwave digestion and determine 36 metallic elements content by ICP-MS with using Rh、Re as internal standard. Results The results showed that all the 36 metallic elements had good linearity in certain rang with the coefficient(r) higher than 0.999 5.The average recoveries were among 86.5% to 107.1%, with the relative standard deviation among 1.2% to 6.1%.The limits of quantification of 36 metallic elements were among 0.001 μg·L~(-1) to 0.968 μg·L~(-1). Conclusion The results demonstrated that the developed method is simple, rapid sensitive and reliable, and suitable for the determination of metallic elements.
Objective To establish a method to determine 36 metallic elements in Ipecacuanhae by microwave digestion-ICP-MS. Methods HNO_3-H_2O_2 was used as the digestion system to treated samples by microwave digestion and determine 36 metallic elements content by ICP-MS with using Rh、Re as internal standard. Results The results showed that all the 36 metallic elements had good linearity in certain rang with the coefficient(r) higher than 0.999 5.The average recoveries were among 86.5% to 107.1%, with the relative standard deviation among 1.2% to 6.1%.The limits of quantification of 36 metallic elements were among 0.001 μg·L~(-1) to 0.968 μg·L~(-1). Conclusion The results demonstrated that the developed method is simple, rapid sensitive and reliable, and suitable for the determination of metallic elements.
引文
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[2] The United States Pharmacopeial Conventio.USA Pharma-copoeia( USP32-NF27) [S].Rockville,USA,2009:2682.
[3] Society of Japanese Pharmacopoeia.The Japanese Pharma-copoeia[S].Tokyo,Japan,2006:1303.
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[5] 关智博,王灿灿,赵尚峰,等.ICP-MS法检测四君子汤中10种金属元素[J].沈阳药科大学学报,2018,35(1):38-42.
[6] 谢志慧,尹智慧,盛振华.基于电感耦合等离子体发射光谱法对不同产地菊花微量元素的含量测定及成分分析[J].医药导报,2015,34(8):1075-1077.
[7] 高秀蓉,许小红,廖昌军,等.中药重金属质量控制研究进展[J].医药导报,2008,27(4):431-433.
[8] 左甜甜,李耀磊,李永鹏,等.电感耦合等离子体质谱法半定量快速筛查13种中药材中重金属及毒性元素[J].药物分析杂志,2017,37(12):2238-2244.
[9] 左甜甜,李耀磊,金红宇,等.ICP-MS法测定18种动物药中重金属及有害元素的残留及初步风险分析[J].药物分析杂志,2017,37(2):237-242.
[10] 金日光,牟雪雁.关于生物高分子元素活性中心分布规律的第四统计力学理论标度的研究(Ⅲ)——论各种化学元素成为生物高分子活性中心元素的先决条件[J].北京化工大学学报,2003,30(4):41-44.
[2] The United States Pharmacopeial Conventio.USA Pharma-copoeia( USP32-NF27) [S].Rockville,USA,2009:2682.
[3] Society of Japanese Pharmacopoeia.The Japanese Pharma-copoeia[S].Tokyo,Japan,2006:1303.
[4] 牛娟,武燕,何华,等.梭梭人工接种肉苁蓉与野生肉苁蓉活性成分及金属元素的含量测定[J].中国现代应用药学,2017,34(5):671-673.
[5] 关智博,王灿灿,赵尚峰,等.ICP-MS法检测四君子汤中10种金属元素[J].沈阳药科大学学报,2018,35(1):38-42.
[6] 谢志慧,尹智慧,盛振华.基于电感耦合等离子体发射光谱法对不同产地菊花微量元素的含量测定及成分分析[J].医药导报,2015,34(8):1075-1077.
[7] 高秀蓉,许小红,廖昌军,等.中药重金属质量控制研究进展[J].医药导报,2008,27(4):431-433.
[8] 左甜甜,李耀磊,李永鹏,等.电感耦合等离子体质谱法半定量快速筛查13种中药材中重金属及毒性元素[J].药物分析杂志,2017,37(12):2238-2244.
[9] 左甜甜,李耀磊,金红宇,等.ICP-MS法测定18种动物药中重金属及有害元素的残留及初步风险分析[J].药物分析杂志,2017,37(2):237-242.
[10] 金日光,牟雪雁.关于生物高分子元素活性中心分布规律的第四统计力学理论标度的研究(Ⅲ)——论各种化学元素成为生物高分子活性中心元素的先决条件[J].北京化工大学学报,2003,30(4):41-44.