2014-2018年广西6种猪主要病毒性繁殖障碍疫病流行病学调查
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摘要
试验旨在了解广西猪主要病毒性繁殖障碍疫病的发生态势,为预防和控制此类疫病提供科学依据。采用RT-PCR/PCR方法对2014年3月至2018年2月采自广西的349份流产胎儿样品进行猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪圆环病毒2型(PCV2)、猪伪狂犬病病毒(PRV)、猪细小病毒(PPV)及猪乙型脑炎病毒(JEV)6种主要病毒性繁殖障碍疫病病原的检测并分析其阳性检出率在不同年份、季节之间的差异及病原混合感染情况。结果显示,2014-2017年,6种病原的猪场阳性率从高到低依次为JEV(36.00%)、PRRSV(16.00%)、PCV2(9.00%)、PRV(7.00%)、PPV(6.00%)和CFSV(5.00%),样品阳性检出率从高到低依次为JEV(27.06%)、PRRSV(6.18%)、PRV(5.88%)、PCV2(3.82%)、CSFV(2.35%)和PPV(2.35%)。季节因素对6种病毒性繁殖障碍疫病的发生有一定影响:JEV在夏季、秋季检出率较高,春季、冬季检出率较低;PRRSV一年四季检出率均较高,且无明显差异;PCV2在春季检出率较高,其次是夏季和冬季,秋季检出率较低;PRV在春季、冬季检出率较高,夏季、秋季检出率均为0;PPV在夏季检出率较高,其次是春季和秋季,冬季检出率为0;CSFV在冬季检出率最高,其次是夏季,春季和秋季检出率均为0。流产胎儿以单一感染为主,6种病毒均存在不同程度单一感染情况,单一感染率JEV最高(23.82%)、PCV2最低(0.59%);混合感染以双重感染为主,其中以2016年双重感染情况较多,且感染组合多样;三重感染情况较少、仅在2016年送检样品中发现1例,未发现四重及四重以上感染情况。调查结果表明,2014-2017年,JEV均有较高的阳性检出率,已成为广西部分猪场母猪流产最主要病毒性病原。PRRSV和PRV仍是目前引起广西猪场母猪流产的主要病毒性病原。
The aim of the study was to understand the occurrence of major viral reproductive disorders of pigs in Guangxi and provide a scientific basis for the prevention and control of such diseases.6 major viral reproductive disease pathogens of swine fever virus(CSFV),porcine reproductive respiratory syndrome virus(PRRSV),porcine circovirus type 2(PCV2),pseudorabies virus(PRV),porcine parvovirus(PPV)and porcine Japanese encephalitis virus(JEV)from 349 aborted fetus samples from March 2014 to February 2018 in Guangxi were collected by RT-PCR/PCR,and the positive detection rate in different years and seasons and the mixed infection of pathogens were analyzed.The results showed that from 2014 to 2017,the positive rate of pig farms of six pathogens from high to low were JEV(36.00%),PRRSV(16.00%),PCV2(9.00%),PRV(7.00%),PPV(6.00%)and CFSV(5.00%);The positive detection rate of samples from high to low were JEV(27.06%),PRRSV(6.18%),PRV(5.88%),PCV2(3.82%),CSFV(2.35%)and PPV(2.35%).Seasonal factors had an impact on the occurrence of six viral reproductive disorders:The detection rate of JEV was higher in summer and autumn,and was lower in spring and winter;The detection rate of PRRSV was higher in all seasons,and there was no significant difference;PCV2 had higher detection rate in spring,followed by summer and winter,and the detection rate was lower in autumn;The detection rate of PRV was higher in spring and winter,and the detection rate was 0 in summer and autumn;The detection rate of PPV was higher in summer,followed by spring and autumn,and the detection rate was 0 in winter;CSFV had highest detection rate in winter,followed by summer,and the detection rate was 0 in spring and autumn.The abortion fetus was dominated by a single infection.The six viruses had different degrees of single infection.The single infection rate was the highest in JEV(23.82%)and the lowest in PCV2(0.59%).The mixed infection was mainly caused by double infection.Among them,the double infection was more in 2016,and the infection combination was diverse.The triple infection was less,only in 2016.One case was found in the samples submitted,and no quadruple or more infections were found.The results showed that from 2014 to 2017,JEV had a high positive detection rate,which had become the most important viral pathogen in sow abortion in some pig farms in Guangxi.PRRSV and PRV still were the main viral pathogens causing abortion in sows in Guangxi.
The aim of the study was to understand the occurrence of major viral reproductive disorders of pigs in Guangxi and provide a scientific basis for the prevention and control of such diseases.6 major viral reproductive disease pathogens of swine fever virus(CSFV),porcine reproductive respiratory syndrome virus(PRRSV),porcine circovirus type 2(PCV2),pseudorabies virus(PRV),porcine parvovirus(PPV)and porcine Japanese encephalitis virus(JEV)from 349 aborted fetus samples from March 2014 to February 2018 in Guangxi were collected by RT-PCR/PCR,and the positive detection rate in different years and seasons and the mixed infection of pathogens were analyzed.The results showed that from 2014 to 2017,the positive rate of pig farms of six pathogens from high to low were JEV(36.00%),PRRSV(16.00%),PCV2(9.00%),PRV(7.00%),PPV(6.00%)and CFSV(5.00%);The positive detection rate of samples from high to low were JEV(27.06%),PRRSV(6.18%),PRV(5.88%),PCV2(3.82%),CSFV(2.35%)and PPV(2.35%).Seasonal factors had an impact on the occurrence of six viral reproductive disorders:The detection rate of JEV was higher in summer and autumn,and was lower in spring and winter;The detection rate of PRRSV was higher in all seasons,and there was no significant difference;PCV2 had higher detection rate in spring,followed by summer and winter,and the detection rate was lower in autumn;The detection rate of PRV was higher in spring and winter,and the detection rate was 0 in summer and autumn;The detection rate of PPV was higher in summer,followed by spring and autumn,and the detection rate was 0 in winter;CSFV had highest detection rate in winter,followed by summer,and the detection rate was 0 in spring and autumn.The abortion fetus was dominated by a single infection.The six viruses had different degrees of single infection.The single infection rate was the highest in JEV(23.82%)and the lowest in PCV2(0.59%).The mixed infection was mainly caused by double infection.Among them,the double infection was more in 2016,and the infection combination was diverse.The triple infection was less,only in 2016.One case was found in the samples submitted,and no quadruple or more infections were found.The results showed that from 2014 to 2017,JEV had a high positive detection rate,which had become the most important viral pathogen in sow abortion in some pig farms in Guangxi.PRRSV and PRV still were the main viral pathogens causing abortion in sows in Guangxi.
引文
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[11]秦毅斌,何颖,何苹萍,等.广西猪乙型脑炎血清流行病学调查分析[J].南方农业学报,2011,42(6):668-671.QIN Y B,HE Y,HE P P,et al.Serological epidemiology of Japanese encephalitis virus infection to swine in Guangxi[J].Southern Agricultural Journal,2011,42(6):668-671.(in Chinese)
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[14]蔡雨函,杨雪,徐志文,等.四川省猪繁殖与呼吸综合征病毒分子流行病学调查[J].中国兽医报,2015,35(2):195-200.CAI Y H,YANG X,XU Z W,et al.Molecular epidemiological investigation of porcine reproductive and respiratory syndrome virus in Sichuan province[J].Chinese Journal of Veterinary Medicine,2015,35(2):195-200.(in Chinese)
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