羊栖菜褐藻酸钠对便秘模型小鼠的润肠通便作用
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摘要
研究羊栖菜褐藻酸钠对便秘模型小鼠的润肠通便作用。将雄性ICR小鼠随机分为空白组、模型组和4个剂量的给药组。给药组分别经口给予30、100、300、1 000 mg(/kg·d)的羊栖菜褐藻酸钠;空白组和模型组经口给予蒸馏水。连续灌胃7天后,观察各组小鼠的首次排黑便的时间、5小时内排黑便的粒数,测定粪便的质量和小肠墨汁推进率。结果表明:羊栖菜褐藻酸钠能显著缩短小鼠首次排黑便的时间,增加小鼠5小时内排黑便的数量、粪便的质量和墨汁推进率,与模型组相比,300 mg(/kg·d)和1 000 mg(/kg·d)剂量组均具有极显著性差异(P<0.01)。
In order to study the laxative effect of Sargassum fusiforme sodium alginate on constipation model mice.Male ICR mice were randomly divided into blank group,model group and alginate-treatment groups.Alginate-treatment group was orally administrated with 30,100,300,1 000 mg(/kg·d)of Sargassum fusiforme sodium alginate;The blank group and model control group were given distilled water. After daily intragastric administration for continuous 7 days,the first time of melena discharge was recorded,and the number of defecations within 5 hours was counted. The weight of faeces and the promotion rate of intestinal ink were measured. The result indicated that Sargassum fusiformis sodium alginate could significantly shorten the time of melena in mice,and also increase the number of defecation,feces weight and intestinal propulsive rate within 5 h,compared wth the moldelgroup,there were significant differences between the 300 mg(/kg·d)and1 000 mg(/kg·d)groupscp(P<0.01).
In order to study the laxative effect of Sargassum fusiforme sodium alginate on constipation model mice.Male ICR mice were randomly divided into blank group,model group and alginate-treatment groups.Alginate-treatment group was orally administrated with 30,100,300,1 000 mg(/kg·d)of Sargassum fusiforme sodium alginate;The blank group and model control group were given distilled water. After daily intragastric administration for continuous 7 days,the first time of melena discharge was recorded,and the number of defecations within 5 hours was counted. The weight of faeces and the promotion rate of intestinal ink were measured. The result indicated that Sargassum fusiformis sodium alginate could significantly shorten the time of melena in mice,and also increase the number of defecation,feces weight and intestinal propulsive rate within 5 h,compared wth the moldelgroup,there were significant differences between the 300 mg(/kg·d)and1 000 mg(/kg·d)groupscp(P<0.01).
引文
[1]牛广财,朱丹,肖盾,等.沙果渣膳食纤维润肠通便作用的动物实验研究[J].食品科学,2011,32(13):293-296
[2]唐伟峰,唐晓军,杨巍.功能性便秘的中西医研究进展[J].世界中西医结合杂志,2015,10(6):880-884
[3]RUIZ-LOPEZ M C,COSS-ADAME A E.Quality of life in patients with different constipation subtypes based on the RomeⅢcriteria[J].Rev Gastroenterol Mex,2015,80(1):13-20
[4]苏碧川,余秀,田成.聚乙二醇电解质散剂联合乳果糖口服液治疗老年性便秘的疗效观察[J].现代药物与临床,2016,31(7):1071-1074
[5]L Liu,M HEINRICH,S MYERS,et al.Towards a better understanding of medicinal uses of the brown seaweed Sargassum in Traditional Chinese Medicine:a phytochemical and pharmacological review[J].Ethnopharmacol,2012,142(3):591-619
[6]CHEN P,HE D,ZHANG Y,et al.Sargassum fusiforme polysaccharides activate antioxidant defense by promoting Nrf2-dependent cytoprotection and ameliorate stress insult during aging[J].Food&Function,2016,7(11):4576-4588
[7]李时珍.本草纲目[M].北京:科学出版社,1957:13-77
[8]李波.羊栖菜褐藻糖胶的提取纯化和结构研究[D].无锡:江南大学,2005:3
[9]PASKALEVA EE,LIN X,DUUS K.Sargassum fusiforme fraction is a potent and specific inhibitor of HIV-1 fusion and reverse transceiptase[J].Virology Journal,2008,5(8):1-9
[10]JI Y,JI C,WANG C.Study on S180 tumor mice erythrocyte membrance function of Sargassum fusiform polysaccharides[C].7th AsianPacific Conference on Medical and Biological Engineering,2008:545-547
[11]刘斌,王长云,管华诗,等.海藻多糖褐藻胶生物活性及其应用研究新进展[J].中国海洋药物杂志,2004,23(6):36-41
[12]TUSI S K,KHALAJ L,ASHABI G,et al.Alginate oligosaccharide protects against endoplasmic reticulum-and mitochondrial-mediated apoptotic cell death and oxidative stress[J].Biomaterials,2011,32(23):5438-5458
[13]FALKEBORGA M,CHEONGA L,GIANFICOA C,et al.Alginate oligosaccharides:Enzymatic preparation and antioxidant property evaluation[J].Food Chemistry,2014,164(20):185-194
[14]DORTERLER ME,AVANGIL HR,Turan C,et al.Comparison of the hemostatic effects of oxidized cellulose and calcium alginate in an experimental animal model of hepatic parenchymal bleeding[J].International journal of critical illness&injury Science,2016,6(4):167-171
[15]MICKA A,SIEPELMEYER A,HOLZ A,et al.Effect of consumption of chicory inulin on bowel function in healthy subjects with constipa tion:a randomized,double-blind,placebo-controlled trial[J].Int JFood Sci Nutr,2017,68(1):82-89
[16]王小蕾,王蔚虹,戴芸,等.益生菌/益生元制剂治疗功能性便秘效果的系统评价和Meta分析[J].临床药物治疗杂志,2014,12(4):33-38
[17]HAN D,IRAGORRI N,CLEMENT F,et al.Cost effectiveness of treatments for chronic constipation:A systematic review[J].Pharmacoeconomics,2018,36(4):435-449
[18]BHARUCHA A E,WOUTERS M M,TACK J.Existing and emerging therapies for managing constipation and diarrhea[J].Current Opinion in Pharmacology,2017,37:158-166
[19]安传峰.从蟪纹雷松藻提取褐藻胶的消化工艺研究[D].北京:中国科学院大学,2017:27-28
[20]BLUMENKARNTZ N,ASBOE H G.New method for quantitative determination of uronic acid[J].Analytical Biochemistry,1973,54(2):484-489
[21]ZOR T,SELINGER Z.Linearization of the Bradford protein assay increases its sensitivity theoretical and experimental studies[J].Analytical Biochemistry,1996,236(2):302-308
[22]中华人民共和国卫生部.保健食品检验与评价技术规范[S].北京:卫生部卫生法制与监督司,2003:159-160
[23]孙春禄.肉制品复合保水剂的研制[J].中国食品添加剂,2009(4):140-146
[2]唐伟峰,唐晓军,杨巍.功能性便秘的中西医研究进展[J].世界中西医结合杂志,2015,10(6):880-884
[3]RUIZ-LOPEZ M C,COSS-ADAME A E.Quality of life in patients with different constipation subtypes based on the RomeⅢcriteria[J].Rev Gastroenterol Mex,2015,80(1):13-20
[4]苏碧川,余秀,田成.聚乙二醇电解质散剂联合乳果糖口服液治疗老年性便秘的疗效观察[J].现代药物与临床,2016,31(7):1071-1074
[5]L Liu,M HEINRICH,S MYERS,et al.Towards a better understanding of medicinal uses of the brown seaweed Sargassum in Traditional Chinese Medicine:a phytochemical and pharmacological review[J].Ethnopharmacol,2012,142(3):591-619
[6]CHEN P,HE D,ZHANG Y,et al.Sargassum fusiforme polysaccharides activate antioxidant defense by promoting Nrf2-dependent cytoprotection and ameliorate stress insult during aging[J].Food&Function,2016,7(11):4576-4588
[7]李时珍.本草纲目[M].北京:科学出版社,1957:13-77
[8]李波.羊栖菜褐藻糖胶的提取纯化和结构研究[D].无锡:江南大学,2005:3
[9]PASKALEVA EE,LIN X,DUUS K.Sargassum fusiforme fraction is a potent and specific inhibitor of HIV-1 fusion and reverse transceiptase[J].Virology Journal,2008,5(8):1-9
[10]JI Y,JI C,WANG C.Study on S180 tumor mice erythrocyte membrance function of Sargassum fusiform polysaccharides[C].7th AsianPacific Conference on Medical and Biological Engineering,2008:545-547
[11]刘斌,王长云,管华诗,等.海藻多糖褐藻胶生物活性及其应用研究新进展[J].中国海洋药物杂志,2004,23(6):36-41
[12]TUSI S K,KHALAJ L,ASHABI G,et al.Alginate oligosaccharide protects against endoplasmic reticulum-and mitochondrial-mediated apoptotic cell death and oxidative stress[J].Biomaterials,2011,32(23):5438-5458
[13]FALKEBORGA M,CHEONGA L,GIANFICOA C,et al.Alginate oligosaccharides:Enzymatic preparation and antioxidant property evaluation[J].Food Chemistry,2014,164(20):185-194
[14]DORTERLER ME,AVANGIL HR,Turan C,et al.Comparison of the hemostatic effects of oxidized cellulose and calcium alginate in an experimental animal model of hepatic parenchymal bleeding[J].International journal of critical illness&injury Science,2016,6(4):167-171
[15]MICKA A,SIEPELMEYER A,HOLZ A,et al.Effect of consumption of chicory inulin on bowel function in healthy subjects with constipa tion:a randomized,double-blind,placebo-controlled trial[J].Int JFood Sci Nutr,2017,68(1):82-89
[16]王小蕾,王蔚虹,戴芸,等.益生菌/益生元制剂治疗功能性便秘效果的系统评价和Meta分析[J].临床药物治疗杂志,2014,12(4):33-38
[17]HAN D,IRAGORRI N,CLEMENT F,et al.Cost effectiveness of treatments for chronic constipation:A systematic review[J].Pharmacoeconomics,2018,36(4):435-449
[18]BHARUCHA A E,WOUTERS M M,TACK J.Existing and emerging therapies for managing constipation and diarrhea[J].Current Opinion in Pharmacology,2017,37:158-166
[19]安传峰.从蟪纹雷松藻提取褐藻胶的消化工艺研究[D].北京:中国科学院大学,2017:27-28
[20]BLUMENKARNTZ N,ASBOE H G.New method for quantitative determination of uronic acid[J].Analytical Biochemistry,1973,54(2):484-489
[21]ZOR T,SELINGER Z.Linearization of the Bradford protein assay increases its sensitivity theoretical and experimental studies[J].Analytical Biochemistry,1996,236(2):302-308
[22]中华人民共和国卫生部.保健食品检验与评价技术规范[S].北京:卫生部卫生法制与监督司,2003:159-160
[23]孙春禄.肉制品复合保水剂的研制[J].中国食品添加剂,2009(4):140-146