武夷岩茶对糖尿病大鼠肠道菌群的调节作用
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摘要
将用链脲佐菌素(STZ)诱导的糖尿病模型大鼠,连续灌胃高、中、低剂量的武夷岩茶和生理盐水,30 d后,采用高通量测序技术对大鼠的肠道菌群进行分析,探讨武夷岩茶对糖尿病大鼠肠道菌群的影响.结果表明:与正常对照组相比,模型对照组大鼠肠道菌群的多样性明显增多(P<0.05),而武夷岩茶可以有效抑制这一趋势,其中以武夷岩茶高剂量组的抑制效果最为显著(P<0.05);与正常对照组相比,模型对照组大鼠肠道菌群从门到属均发生显著变化,其中,拟杆菌门的比例显著下降(P<0.05),变形菌门的比例显著上升(P<0.05).灌胃武夷岩茶后,拟杆菌门的比例上升,变形菌门的比例下降,且调节效果与武夷岩茶的剂量呈正相关.提示武夷岩茶具有改善糖尿病大鼠肠道菌群结构的作用.
Diabetic rats induced by streptozotocin(STZ) were treated with continuously intragastric administration at 3 levels of Wuyi rock tea, with the addition of saline to normal and diabetic rats as the control and model groups, respectively. After 30 d, the intestinal flora of rats were analyzed by high-throughput sequencing. The results showed that the intestinal flora diversity of the model group increased significantly compared with the control(P<0.05). Rock tea effectively suppressed this trend, with the inhibitory effect from the high-dose being the strongest(P<0.05). Compared with the control, the intestinal flora of the model group at phylum and genus level changed significantly, with a decreased proportion of Bacteriodetes and increased proportion of Proteobacteria(P<0.05). In contrast, the proportion of Bacteroidetes in groups with rock tea intake increased and the proportion of Proteobacteria decreased. Moreover, the regulatory effect of Wuyi rock tea was positively related to the dose. Therefore, Wuyi rock tea is likely to adjust the imbalance intestinal flora attributed to diabetes.
Diabetic rats induced by streptozotocin(STZ) were treated with continuously intragastric administration at 3 levels of Wuyi rock tea, with the addition of saline to normal and diabetic rats as the control and model groups, respectively. After 30 d, the intestinal flora of rats were analyzed by high-throughput sequencing. The results showed that the intestinal flora diversity of the model group increased significantly compared with the control(P<0.05). Rock tea effectively suppressed this trend, with the inhibitory effect from the high-dose being the strongest(P<0.05). Compared with the control, the intestinal flora of the model group at phylum and genus level changed significantly, with a decreased proportion of Bacteriodetes and increased proportion of Proteobacteria(P<0.05). In contrast, the proportion of Bacteroidetes in groups with rock tea intake increased and the proportion of Proteobacteria decreased. Moreover, the regulatory effect of Wuyi rock tea was positively related to the dose. Therefore, Wuyi rock tea is likely to adjust the imbalance intestinal flora attributed to diabetes.
引文
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[21] 陈佩,党辉,张秋香,等.鼠李糖乳酸杆菌对高脂饮食和链脲霉素诱发的Ⅱ型糖尿病小鼠的降糖作用[J].食品工业科技,2013,34(24):351-354.
[2] 朱超霞,陆颖理.肠道菌群与肥胖及相关代谢性疾病关系的研究进展[J].上海交通大学学报(医学版),2014,34(12):1 829-1 833.
[3] 肖叔敏,于珮.肠道菌群与2型糖尿病及并发症[J].世界华人消化杂志,2017(20):1 819-1 831.
[4] 蓝雪铭.武夷岩茶对SPF级大鼠肠道菌群的影响[D].福州:福州大学,2013.
[5] 马森,陈培珍,游玉琼,等.武夷岩茶降血糖作用的研究[J].武夷学院学报,2007,26(4):24-26.
[6] 金莉莎,刘仲华,黄建安,等.大红袍与红茶对肠道菌群失衡的作用研究[J].食品与机械,2013(3):1-3.
[7] 许勇泉,尹军峰,沈丹玉,等.武夷岩茶饮料最佳浸提参数的研究[J].浙江农业科学,2009,1(6):1 149.
[8] 庞玉萍.绞股蓝总皂苷对2型糖尿病大鼠游离脂肪酸代谢的影响及改善胰岛素抵抗相关机制研究[J].中华中医药学刊,2016,34(4):960-963.
[9] 郭静.速溶普洱茶对2型糖尿病小鼠的降血糖作用研究[D].昆明:昆明理工大学,2012.
[10] 王雪,赵龙玉,赵凤春,等.应用Illumina高通量测序技术探究长根菇多糖对小鼠肠道菌群的影响[J].食品科学,2015,36(19):222-226.
[11] RC E. UPARSE: highly accurate OTU sequences from microbial amplicon reads [J]. Nature Methods, 2013,10(10):996-998.
[12] LI B, ZHANG X, GUO F, et al. Characterization of tetracycline resistant bacterial community in saline activated sludge using batch stress incubation with high-throughput sequencing analysis [J]. Water Research, 2013,47(13):4 207-4 216.
[13] 张芹,周中凯,张惠媛.荞麦壳水提物调控糖尿病大鼠肠道菌群结构的研究[J].中国粮油学报,2017,32(4):31-36.
[14] 李先峰,王影,李贞贞,等.肠道菌群与糖尿病相关性研究进展[J].中国微生态学杂志,2015,27(10):1 224-1 228.
[15] 肖党生,王招娣,杨云梅,等.糖尿病患者肠道菌群研究[J].中国微生态学杂志,2006,18(4):275-276.
[16] 刘冬恋,廖梦玲,周欢.基于高通量测序技术分析糖尿病与肠道菌群相关性的研究进展[J].生物技术通报,2016,32(9):59-64.
[17] 张芹,周中凯,任晓冲,等.高通量测序技术研究糖尿病大鼠与正常大鼠粪便菌群的结构差异[J].中国食品学报,2017,17(6):232-239.
[18] 王雯玲.黄连花薹对高血糖大鼠肠道菌群作用的研究[D].武汉:华中科技大学,2015.
[19] LARSEN N, VOGENSEN F K, FW V D B, et al. Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults [J]. PLoS ONE, 2010,5(2):e9085.
[20] 刘婷婷.实时定量PCR法分析2型糖尿病患者肠道乳酸杆菌菌种数量的改变[D].广东:南方医科大学,2012.
[21] 陈佩,党辉,张秋香,等.鼠李糖乳酸杆菌对高脂饮食和链脲霉素诱发的Ⅱ型糖尿病小鼠的降糖作用[J].食品工业科技,2013,34(24):351-354.