丹栀逍遥散联合愈乳癖汤对乳腺增生模型小鼠的治疗作用
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摘要
目的:观察逍遥散联合愈乳癖汤对乳腺增生模型小鼠的治疗作用及对小鼠血清相关激素水平的影响。方法:采用苯甲酸雌二醇和孕激素肌肉注射建立小鼠乳腺增生模型,将雌性小鼠随机分为对照组,模型组和药物组。使用立体定量分析的方法观察小鼠乳腺小叶体密度、乳腺小叶间质体密度和腺泡比表面积,采用酶联免疫法检测小鼠血清雌二醇和黄体酮水平,计算相关脏器指数,光学显微镜下统计乳腺泡数和乳腺导管层数。结果:与模型组比较,药物组的乳腺小叶体密度和腺泡比表面积显著下降(P<0.05),乳腺小叶间质体密度显著上升(P<0.05),乳腺泡数和乳腺导管层数均得到改善。与模型组比较,逍遥散联合愈乳癖汤可显著降低乳腺增生模型小鼠血清的雌二醇水平(P<0.01),明显增加黄体酮(P<0.05)水平,并能够改善相关脏器指数(P<0.05)。结论:逍遥散联合愈乳癖汤能够改善小鼠的乳腺增生,降低雌二醇水平,升高黄体酮水平。
Objective:To observe the therapeutic effect of Xiaoyao Powder combined with Yurupi Decoction on mouse with mammary gland hyperplasia and its effect on serum related hormone levels.Methods:Mammary gland hyperplasia model was established by intramuscular injection of estradiol benzoate and progesterone.Female mice were randomly divided into control group,model group and Xiaoyao Powder combined with Yurupi Decoction group.Stereoscopic quantitative analysis was used to observe the density of mammary gland lobule,mammary gland interstitial density and acinar specific surface area.The serum estradiol and progesterone levels were detected by enzyme-linked immunosorbent assay(ELISA),and the relevant organ index was calculated.The number of mammary follicles and the number of mammary duct layers were counted.Results:Compared with that of the model group,the lobular body density and acinar specific surface area of Xiaoyao Powder combined with Yuyao Decoction group decreased significantly(P<0.05),and the interstitial density of mammary gland increased significantly(P<0.05).Both the number of layers of the mammary ducts was improved.Compared with that of the model group,Xiaoyao Powder combined with Yurupi Decoction can significantly reduce the serum estradiol level in mice with hyperplasia of mammary gland(P<0.01),the level of progesterone significantly increased(P<0.05) and the relevant organ index was significantly improved(P<0.05).Conclusion:Xiaoyao Powder combined with Yurupi Decoction can improve mammary gland hyperplasia,reduce the estradiol level and increase the progesterone level.
Objective:To observe the therapeutic effect of Xiaoyao Powder combined with Yurupi Decoction on mouse with mammary gland hyperplasia and its effect on serum related hormone levels.Methods:Mammary gland hyperplasia model was established by intramuscular injection of estradiol benzoate and progesterone.Female mice were randomly divided into control group,model group and Xiaoyao Powder combined with Yurupi Decoction group.Stereoscopic quantitative analysis was used to observe the density of mammary gland lobule,mammary gland interstitial density and acinar specific surface area.The serum estradiol and progesterone levels were detected by enzyme-linked immunosorbent assay(ELISA),and the relevant organ index was calculated.The number of mammary follicles and the number of mammary duct layers were counted.Results:Compared with that of the model group,the lobular body density and acinar specific surface area of Xiaoyao Powder combined with Yuyao Decoction group decreased significantly(P<0.05),and the interstitial density of mammary gland increased significantly(P<0.05).Both the number of layers of the mammary ducts was improved.Compared with that of the model group,Xiaoyao Powder combined with Yurupi Decoction can significantly reduce the serum estradiol level in mice with hyperplasia of mammary gland(P<0.01),the level of progesterone significantly increased(P<0.05) and the relevant organ index was significantly improved(P<0.05).Conclusion:Xiaoyao Powder combined with Yurupi Decoction can improve mammary gland hyperplasia,reduce the estradiol level and increase the progesterone level.
引文
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[2]胡霖霖,张永华.百合逍遥散对抑郁模型大鼠行为学及海马5-HT、NE的影响[J].中医学报,2015,30(3):399-401.
[3]HUANG J,TANG H,CAO S,et al.Molecular Targets and Associated Potential Pathways of Danlu Capsules in Hyperplasia of Mammary Glands Based on Systems Pharmacology[J].Afr J Tradit Complement Altern Med,2017,14(4):247-252.
[4]KONSTANTINOVA A M,SHELEKHOVA K V.Fibroepithelial tumors of the anogenital mammary-like glands[J].Arkh Patol,2017,79(4):40-47
[5]KONSTANTINOVA A M,SPAGNOLO D V,STEWARTC J R,et al.Spectrum of Changes in Anogenital Mammary-like Glands in Primary Extramammary (Anogenital) Paget Disease and Their Possible Role in the Pathogenesis of the Disease[J].Am J Surg Pathol,2017,41(8):1053-1058.
[6]LI X,XIN P,WANG C,et al.Mechanisms of Traditional Chinese Medicine in the Treatment of Mammary Gland Hyperplasia[J].Am J Chin Med,2017,45(3):443-458.
[7]JIA Y,LIU X,JIA Q,et al.The anti-hyperplasia of mammary gland effect of protein extract HSS from Tegillarca granosa[J].Biomed Pharmacother,2017,85(1):1-6.
[8]BURRAI G P,TANCA A,CUBEDDU T,et al.A first immunohistochemistry study of transketolase and transketolase-like 1 expression in canine hyperplastic and neoplastic mammary lesions[J].BMC Vet Res,2017,13(1):38-42.
[9]NOELIA M,EVA R L,CAROLINA P,et al.A dioxin-like compound induces hyperplasia and branching morphogenesis in mouse mammary gland,through alterations in TGF-β1 and aryl hydrocarbon receptor signaling[J].Toxicol Appl Pharmacol,2017,155(4):277-285.