一株疑似野生大白口蘑的鉴定及菌丝培养基筛选
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摘要
【目的】鉴定 1 株在粤北韶关学院内采集的疑似大白口蘑(Tricholoma giganteum)野生菌株,并筛选其菌丝适宜培养基,为丰富其生物资源、开发利用该菌株提供依据和参考。【方法】以该疑似大白口蘑野生菌株为试材,采用组织分离法,从野生子实体中分离纯化得到纯菌丝体;通过子实体形态特征分析、菌丝生物学特性分析和ITS序列克隆与分析对其进行鉴定;并在PSA培养基、酵母膏培养基和棉籽壳培养基中进行菌丝培养试验,筛选菌丝适宜培养基。【结果】该菌株与GenBank中报道的大白口蘑具有较高的相似性、Ident在82.23%～90.06%之间,其中与序列JX041888.1相似性最高、Ident为90.06%。结合传统形态学分析,鉴定该菌株为野生大白口蘑,命名为Tsg1。菌丝体均可在PSA培养基、酵母膏培养基和棉籽壳培养基中培养,但在棉籽壳培养基中菌丝体最为浓密粗壮、长势最好,其菌丝生长速度为3.68 mm/d。【结论】该菌株为野生大白口蘑,棉籽壳培养基是其菌丝培养适宜培养基。
【Objective】A wild strain of suspected Tricholoma giganteum collected in Shaoguan University,Northern Guangdong Province was identified and the suitable mycelium culture medium was screened, which provided basis and reference for the enrichment of its biological resources and the development and utilization of the strain. 【Method】Using the wild strain of the suspected T. giganteum as tested material, the pure mycelium was isolated and purified by tissue isolation from the wild strain fruiting body. Identification was carried out by the morphological characteristics analysis of fruiting bodies, biological characteristics analysis of mycelium, and ITS sequence cloning and analysis. In order to screen the most suitable mycelium culture medium, mycelium culture experiment was carried out in PSA medium, yeast extract medium and cottonseed shell medium, respectively.【Result】The cloning and analysis of ITS sequence showed that the suspected wild T. giganteum had high similarity with the T. giganteum that reported in GenBank and Ident was between 82.23% and 90.06%, The sequence showed highest similarity with sequence JX041888.1 and its Ident was 90.06%. Combined with traditional morphological analysis, the strain was identified as wild T. giganteum and named as Tsg1. The mycelium could be cultured in PSA medium, yeast extract medium and cottonseed shell medium. But the mycelium was the densest and the strongest in the cottonseed shell medium, with the growth rate of 3.68 mm/d.【Conclusion】This strain is a wild T. giganteum, and cottonseed shell medium is the most suitable medium for mycelium culture.
【Objective】A wild strain of suspected Tricholoma giganteum collected in Shaoguan University,Northern Guangdong Province was identified and the suitable mycelium culture medium was screened, which provided basis and reference for the enrichment of its biological resources and the development and utilization of the strain. 【Method】Using the wild strain of the suspected T. giganteum as tested material, the pure mycelium was isolated and purified by tissue isolation from the wild strain fruiting body. Identification was carried out by the morphological characteristics analysis of fruiting bodies, biological characteristics analysis of mycelium, and ITS sequence cloning and analysis. In order to screen the most suitable mycelium culture medium, mycelium culture experiment was carried out in PSA medium, yeast extract medium and cottonseed shell medium, respectively.【Result】The cloning and analysis of ITS sequence showed that the suspected wild T. giganteum had high similarity with the T. giganteum that reported in GenBank and Ident was between 82.23% and 90.06%, The sequence showed highest similarity with sequence JX041888.1 and its Ident was 90.06%. Combined with traditional morphological analysis, the strain was identified as wild T. giganteum and named as Tsg1. The mycelium could be cultured in PSA medium, yeast extract medium and cottonseed shell medium. But the mycelium was the densest and the strongest in the cottonseed shell medium, with the growth rate of 3.68 mm/d.【Conclusion】This strain is a wild T. giganteum, and cottonseed shell medium is the most suitable medium for mycelium culture.
引文
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