复方罗汉果清肺糖浆HPLC特征图谱及主要成分含量测定
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摘要
目的建立复方罗汉果清肺糖浆的特征图谱,并测定其中主要成分木犀草苷、罗汉果皂苷Ⅴ的含量。方法采用Waters Sunfire C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.2%磷酸水溶液为流动相,梯度洗脱,流速0.8 mL/min,检测波长210 nm,柱温25℃,进样量10μL。结果 15批复方罗汉果清肺糖浆HPLC特征图谱确立了10个共有峰,且相对保留时间的RSD均小于2.0%。木犀草苷、罗汉果皂苷Ⅴ分别在0.163 2～0.816 0μg、0.730 8～3.654 0μg范围内线性关系良好,平均加样回收率分别为96.30%、95.11%,RSD分别为2.28%、2.87%。结论 HPLC特征图谱结合主要成分含量测定能更全面反映制剂内在质量,可用于复方罗汉果清肺糖浆综合质量评价。
Objective To establish characteristic spectrum of Fufang Luohanguo Qingfei Syrup by HPLC; To determine the contents of luteoloside and mogroside Ⅴ. Methods HPLC was performed on Waters Sunfire C18 column(4.6 mm × 250 mm, 5 μm) by using acetonitrile and 0.2% phosphoric acid solution as the mobile phase in gradient elution. The flow rate was 0.8 mL/min; the detection wavelength was set at 210 nm; the column temperature was 25 ℃; the injection volume was 10 μL. Results Ten common peaks were determined from the HPLC characteristic spectrum of fifteen batches of samples. The RSDs of retention time were less than 2.0%. The good linear range of luteoloside and mogroside Ⅴ was among 0.163 2–0.816 0 μg and 0.730 8–3.654 0 μg, respectively.The average recovery of sample addition was 96.30% and 95.11%, with RSD of 2.28% and 2.87%, respectively.Conclusion The HPLC characteristic spectrum combined with the content determination of main components can reflect the inherent quality of preparation more comprehensively, which can be used in the comprehensive quality evaluation of Fufang Luohanguo Qingfei Syrup.
Objective To establish characteristic spectrum of Fufang Luohanguo Qingfei Syrup by HPLC; To determine the contents of luteoloside and mogroside Ⅴ. Methods HPLC was performed on Waters Sunfire C18 column(4.6 mm × 250 mm, 5 μm) by using acetonitrile and 0.2% phosphoric acid solution as the mobile phase in gradient elution. The flow rate was 0.8 mL/min; the detection wavelength was set at 210 nm; the column temperature was 25 ℃; the injection volume was 10 μL. Results Ten common peaks were determined from the HPLC characteristic spectrum of fifteen batches of samples. The RSDs of retention time were less than 2.0%. The good linear range of luteoloside and mogroside Ⅴ was among 0.163 2–0.816 0 μg and 0.730 8–3.654 0 μg, respectively.The average recovery of sample addition was 96.30% and 95.11%, with RSD of 2.28% and 2.87%, respectively.Conclusion The HPLC characteristic spectrum combined with the content determination of main components can reflect the inherent quality of preparation more comprehensively, which can be used in the comprehensive quality evaluation of Fufang Luohanguo Qingfei Syrup.
引文
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[10]屠万倩,刘晓苗,张海波,等.HPLC法同时测定不同产地的菊花中8种成分的含量[J].中药材,2018,41(1):152-155.
[11]汤庆发,谢颖,陈飞龙,等.苦杏仁中苦杏仁苷的存在形式及其影响因素[J].中国实验方剂学杂志,2013,19(8):107-109.
[2]GUAN R W,QU Y S,GU Z W,et al.Study on the pharmacologic action of luteoloside[J].Medicinal Plant,2014,5(7):5-7.
[3]沈智,陈丽珏,张文婷,等.RP-HPLC法测定菊花中3,5-O-双咖啡酰基奎宁酸、木犀草苷和绿原酸[J].中草药,2010,41(2):307-310.
[4]武琳,黄菲,杨光.复方罗布麻颗粒中木犀草苷含量测定方法研究[J].中国中医药信息杂志,2015,22(4):91-93.
[5]YAN H,TAO L,QU X,et al.Quantitative determination of mogroside V in rat plasma by LC-MS/MS and its application to a pharmacokinetic study[J].Pak J Pharm Sci,2018,31(3):867-873.
[6]SHEN Y,LIN S J,HAN C,et al.Rapid identification and quantification of five major mogrosides in Siraitia grosvenorii(Luo-Han-Guo)by high performance liquid chromatography-triple quadrupole linear ion trap tandem mass spectrometry combined with microwave-assisted extraction[J].Microchemical Journal,2014,116:142-150.
[7]陈薇,段小群,吴卫,等.富硒罗汉果中罗汉果皂苷Ⅴ的HPLC测定[J].湖北农业科学,2015,54(2):444-446.
[8]师凤华,赵祥升,莫乔程,等.不同产地不同种质金银花中绿原酸和木犀草苷的含量分析[J].中药材,2014,37(7):1145-1148.
[9]何燕宁,赵引利,杨冬丽,等.不同产地及采收期的葎草中木犀草苷、芹菜素-7-O-β-D-葡萄糖苷、木犀草素定量比较研究[J].中草药,2016,47(20):3707-3711.
[10]屠万倩,刘晓苗,张海波,等.HPLC法同时测定不同产地的菊花中8种成分的含量[J].中药材,2018,41(1):152-155.
[11]汤庆发,谢颖,陈飞龙,等.苦杏仁中苦杏仁苷的存在形式及其影响因素[J].中国实验方剂学杂志,2013,19(8):107-109.