苦参碱对HepG2细胞代谢水平和基因水平的影响
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摘要
目的:探讨苦参碱对HepG2细胞代谢水平及基因水平的影响。方法:使用不同浓度的苦参碱作用HepG2细胞3天,对PCNA、AFP、N-ar s、e-myc、p53、Rb进行免疫组化检测,对e-myc、p53 mRNA进行原位杂交法检测,对HSGGT进行亲和微柱法检测。结果:苦参碱用药3天后,苦参碱0.8g/L时HepG2细胞AFP和PCNA表达有所减弱,用药1.5g/L时HepG2细胞AFP和PCNA表达为阴性;用药后HepG2细胞相关代谢指标HSGGT明显活性下降,癌基因e-myc表达减弱,抑癌基因p53、Rb表达增强。结论:苦参碱具有抑制人肝癌HepG2细胞增殖的作用,能影响HepG2细胞的增殖代谢及抑制癌基因的增殖。
Objective To study the Effect of matrine on the metabolic level and the level of gene in HepG2 cells.Methods HepG2 cells were cultured about 3 days in different concentrations of matrine,PCNA,AFP,N-ars,e-myc,p53,Rb were detected by immunohistochemistry,e-myc,p53 mRNA by situ hybridization assay,HSGGT by pro-and micro-column method.Results After 3d,at 0.8g / L of matrine,AFP and PCNA expression weakened at 1.5g / L,AFP and PCNA were negative;The activity of HSGGT decreased obviously,expression of the oncogene of the e-myc decreased,expression of tumor suppressor gene p53 and Rb increased.Conclusion Matrine can affect the proliferation of HepG2 cells metabolism and inhibit the proliferation of cancer genes.
Objective To study the Effect of matrine on the metabolic level and the level of gene in HepG2 cells.Methods HepG2 cells were cultured about 3 days in different concentrations of matrine,PCNA,AFP,N-ars,e-myc,p53,Rb were detected by immunohistochemistry,e-myc,p53 mRNA by situ hybridization assay,HSGGT by pro-and micro-column method.Results After 3d,at 0.8g / L of matrine,AFP and PCNA expression weakened at 1.5g / L,AFP and PCNA were negative;The activity of HSGGT decreased obviously,expression of the oncogene of the e-myc decreased,expression of tumor suppressor gene p53 and Rb increased.Conclusion Matrine can affect the proliferation of HepG2 cells metabolism and inhibit the proliferation of cancer genes.
引文
[1]薛爱华,宋文静.苦参碱药理作用研究概况[J].天津药学,2010,22(5):62-64.
[2]司维柯,李鹏,王源,等.苦参碱抗人肝癌细胞株HepG2的作用及其机制研究[J].国外医学临床生物化学与检验学分册,2003,24(5):288-289.
[3]朱丹丹,姚树坤,闫建国,等.苦参碱对人类肝癌细胞HepG2增殖、细胞周期及凋亡的影响[J].肿瘤研究与临床,2010,22(11):745-747.
[4]邹姝姝,杨帆,陈鸿飞,等.苦参碱对肝癌细胞的作用[J].重庆工学院学报(自然科学),2009,23(11):47-50.
[5]刘福丹,吕燕青,叶开和,等.FR/MA对HepG2肝癌细胞侵袭能力的影响[J].实用肿瘤杂志,2009,24(4):337-340.
[2]司维柯,李鹏,王源,等.苦参碱抗人肝癌细胞株HepG2的作用及其机制研究[J].国外医学临床生物化学与检验学分册,2003,24(5):288-289.
[3]朱丹丹,姚树坤,闫建国,等.苦参碱对人类肝癌细胞HepG2增殖、细胞周期及凋亡的影响[J].肿瘤研究与临床,2010,22(11):745-747.
[4]邹姝姝,杨帆,陈鸿飞,等.苦参碱对肝癌细胞的作用[J].重庆工学院学报(自然科学),2009,23(11):47-50.
[5]刘福丹,吕燕青,叶开和,等.FR/MA对HepG2肝癌细胞侵袭能力的影响[J].实用肿瘤杂志,2009,24(4):337-340.