高效光合细菌菌剂对土壤微生物的影响
|
摘要
为了筛选在盆栽试验条件下适宜的高效光合细菌菌剂浓度以促进番茄生长,研究了高效光合细菌菌剂的3种浓度对番茄种植土壤中微生物特性的影响。试验结果表明:高效光合细菌菌剂的施用可以显著提高土壤中细菌和放线菌的数量,降低土壤中真菌数量;不同程度提高土壤脲酶、酸性磷酸酶和过氧化氢酶的活性。其中以稀释100倍的光合细菌菌剂+化肥处理效果最明显,稀释100倍的菌剂+化肥处理最有利于土壤中微生物的生长繁殖,对土壤环境的改善效果最显著。
In order to select a suitable photosynthetic bacteria inoculants concentration to improve the tomato growth with pot experiment,we studied the effects of three concentrations of high efficient photosynthetic bacteria inoculants on soil microbial properties. The experimental results showed that,significant increases was observed in the population of soil bacteria and soil Actinomycetes with photosynthetic bacteria inoculants,and prominent decrease in that of soil fungi. Soil urease,acid phosphatase and catalase activities were all improved by the application of photosynthetic bacterial inoculants. The photosynthetic bacterial inoculants diluted 100 times plus chemical treatment was the most remarkable in the experiment,which was more conducive to the soil microbes and environment.
In order to select a suitable photosynthetic bacteria inoculants concentration to improve the tomato growth with pot experiment,we studied the effects of three concentrations of high efficient photosynthetic bacteria inoculants on soil microbial properties. The experimental results showed that,significant increases was observed in the population of soil bacteria and soil Actinomycetes with photosynthetic bacteria inoculants,and prominent decrease in that of soil fungi. Soil urease,acid phosphatase and catalase activities were all improved by the application of photosynthetic bacterial inoculants. The photosynthetic bacterial inoculants diluted 100 times plus chemical treatment was the most remarkable in the experiment,which was more conducive to the soil microbes and environment.
引文
[1]王超,吴凡,刘训理,等.不同肥力条件下烟草根际微生物的初步研究[J].中国烟草科学,2005,2:12-14.
[2]贾志红,孙敏,杨珍平,等.施肥对作物根际微生物的影响[J].作物学报,2004,5:491-495.
[3]关松荫.土壤酶及其研究法[M].北京:中国农业出版社,1986,274-323.
[4]薛立,陈红跃,邝立刚.湿地松混交林地土壤养分、微生物和酶活性的研究[J].应用生态学报,2003,1:157-159.
[5]Paul E A,Clack F E.Soil Microbiology and Biochemistry[M].USA San Diego CA:Academic Press,1996,104-109.
[6]Elizabeth A F,Dan B,David V.Soil chemistry changes after27 years and under four tree species in southern Ontario[J].Can.J.Forest Res.,1989,19(12):1648-1650.
[7]曾玲玲,张兴梅,洪音,等.长期施肥与耕作方式对土壤酶活性的影响[J].中国土壤与肥料,2008,2:27-30,60.
[8]徐芬芬,俞晓凤.光合细菌的光合作用及应用展望[J].生物学教学,2011,6:2-3.
[9]John A H.Photosynthetic suspended-growth systems in aquaculture[J].Aqua.Engin.,2006,34(3):344-363.
[10]Gomelsky M,Zeilstra-Ryalls J H.The living genome of a purple nonsulfur photosynthetic bacterium:Overview of the Rhodobacter sphaeroides transcriptome landscapes[J].Adv.Bot.Res.,2013,66:179-203.
[11]Oleg S P,Raul E M,Elena I K,et al..Interaction of metals and protons with anoxygenic phototrophic bacteria Rhodobacter blasticus[J].Chem.Geol.,2013,335(6):75-86.
[12]Cai J L,Wang G C.Hydrogen production by a marine photosynthetic bacterium,Rhodovulum sulfidophilum P5,isolated from a shrimp pond[J].Int.J.Hydr.Energy,2012,37:15070-15080.
[13]赵微,张光明.光合细菌处理废水过程中生物产氢技术研究进展[J].中国生物工程杂志,2012,32(12):130-135.
[14]李军,张辉,李建文,等.光合细菌菌剂在提高板蓝根品质中的应用研究[J].中国医药导报,2011,8(13):58-59.
[15]陈颖,孙红文,张峻,等.光合细菌的固定化及对养殖水体的净化作用[J].水产科技情报,2011,5:234-238.
[16]张松柏,张德咏,刘勇,等.光合细菌PSB07-15对水培黄瓜体系中甲氰菊酯污染的生物修复[J].农业环境科学学报,2009,28(10):2198-2203.
[17]Tim H,Damien J B,Jürg K.Phototrophic bacteria for nutrient recovery from domestic wastewater[J].Water Res.,2014,50:18-26.
[18]Kong F Y,Wang A J,Ren H Y,et al..Improved dechlorination and mineralization of 4-chlorophenol in a sequential biocathode-bioanode bioelectrochemical system with mixed pho-tosynthetic bacteria[J].Bioresour.Technol.,2014,158:32-38.
[19]da Silva M L B,Mezzari M P,Ibelli A M G,et al..Sulfide removal from livestock biogas by Azospirillum-like anaerobic phototrophic bacteria consortium[J].Int.Biodeter.Biodegrad.,2014,86:248-251.
[20]田俊岭,彭桂香,李永涛,等.一种高效光合菌剂对辣椒生长及土壤微生物的影响[J].生物技术进展,2014,3:197-200.
[21]Gorlenko V M.History of the study of biodiversity of photosynthetic bacteria[J].Microbiology,2004,73(5):541-550.
[22]王馥迪,李征,黄秋生,等.光合细菌在植物上的应用现状及展望[J].广东蚕业,2012,2:36-38.
[23]谢修志,谢向坚.光合细菌菌剂对蔬菜品质影响的初报[J].广东农业科学,2009,12:86-87.
[24]史清亮,贺跃武,马玉珍,等.光合细菌在农业上的应用研究[J].山西农业科学,2000,28(2):59-62.
[25]中国科学院南京土壤研究所微生物室.土壤微生物研究法[M].北京:科学出版社,1985,54-57.
[26]张信娣,曹慧,徐冬青,等.光合细菌和有机肥对土壤主要微生物类群和土壤酶活性的影响[J].土壤,2008,40(3):443-447.
[27]张信娣,史永军,陈银科.光合细菌和有机肥对土壤主要微生物类群的影响[J].中国土壤与肥料,2007,3:59-62.
[28]杨芳,田俊岭,杨盼盼,等.高效光合细菌菌剂对番茄品质、土壤肥力及微生物特性的影响[J].华南农业大学学报,2014,35(1):49-54.
[29]何云辉.光合细菌的培养及其对土壤肥力的影响[D].成都:四川师范大学,硕士学位论文,2014,41-43.
[30]Gianfreda L,Sannino F,Vtoante A.Pesticide effects on the activity of free,immobilized and soil invertase[J].Soil Biol.Biochem.,1995,27(9):1201-1208.
[31]王桂芳.沼液配施钾肥对苹果园土壤酶活性和土壤微生物数量及果实品质的影响[D].陕西杨凌:西北农林科技大学,硕士学位论文,2009,14-16.
[32]蒲铃铃,刘桦,曾莉萍.光合细菌对土壤主要微生物类群的影响试验分析[J].生物技术世界,2012,4:11,27.
[33]白红娟,肖根林,贾万利,等.光合细菌提高污染土壤中酶活性的研究[J].工业安全与环保,2011,6:13-15.
[34]肖根林,白红娟,贾万利.光合细菌提高铅、镉及呋喃丹复合污染土壤中酶活性的研究[J].化工技术与开发,2011,2:47-50.
[35]王延军,宗良纲,李锐,等.不同肥料对有机栽培番茄生长和土壤酶及微生物量的影响[J].南京农业大学学报,2007,30(3):83-87.
[2]贾志红,孙敏,杨珍平,等.施肥对作物根际微生物的影响[J].作物学报,2004,5:491-495.
[3]关松荫.土壤酶及其研究法[M].北京:中国农业出版社,1986,274-323.
[4]薛立,陈红跃,邝立刚.湿地松混交林地土壤养分、微生物和酶活性的研究[J].应用生态学报,2003,1:157-159.
[5]Paul E A,Clack F E.Soil Microbiology and Biochemistry[M].USA San Diego CA:Academic Press,1996,104-109.
[6]Elizabeth A F,Dan B,David V.Soil chemistry changes after27 years and under four tree species in southern Ontario[J].Can.J.Forest Res.,1989,19(12):1648-1650.
[7]曾玲玲,张兴梅,洪音,等.长期施肥与耕作方式对土壤酶活性的影响[J].中国土壤与肥料,2008,2:27-30,60.
[8]徐芬芬,俞晓凤.光合细菌的光合作用及应用展望[J].生物学教学,2011,6:2-3.
[9]John A H.Photosynthetic suspended-growth systems in aquaculture[J].Aqua.Engin.,2006,34(3):344-363.
[10]Gomelsky M,Zeilstra-Ryalls J H.The living genome of a purple nonsulfur photosynthetic bacterium:Overview of the Rhodobacter sphaeroides transcriptome landscapes[J].Adv.Bot.Res.,2013,66:179-203.
[11]Oleg S P,Raul E M,Elena I K,et al..Interaction of metals and protons with anoxygenic phototrophic bacteria Rhodobacter blasticus[J].Chem.Geol.,2013,335(6):75-86.
[12]Cai J L,Wang G C.Hydrogen production by a marine photosynthetic bacterium,Rhodovulum sulfidophilum P5,isolated from a shrimp pond[J].Int.J.Hydr.Energy,2012,37:15070-15080.
[13]赵微,张光明.光合细菌处理废水过程中生物产氢技术研究进展[J].中国生物工程杂志,2012,32(12):130-135.
[14]李军,张辉,李建文,等.光合细菌菌剂在提高板蓝根品质中的应用研究[J].中国医药导报,2011,8(13):58-59.
[15]陈颖,孙红文,张峻,等.光合细菌的固定化及对养殖水体的净化作用[J].水产科技情报,2011,5:234-238.
[16]张松柏,张德咏,刘勇,等.光合细菌PSB07-15对水培黄瓜体系中甲氰菊酯污染的生物修复[J].农业环境科学学报,2009,28(10):2198-2203.
[17]Tim H,Damien J B,Jürg K.Phototrophic bacteria for nutrient recovery from domestic wastewater[J].Water Res.,2014,50:18-26.
[18]Kong F Y,Wang A J,Ren H Y,et al..Improved dechlorination and mineralization of 4-chlorophenol in a sequential biocathode-bioanode bioelectrochemical system with mixed pho-tosynthetic bacteria[J].Bioresour.Technol.,2014,158:32-38.
[19]da Silva M L B,Mezzari M P,Ibelli A M G,et al..Sulfide removal from livestock biogas by Azospirillum-like anaerobic phototrophic bacteria consortium[J].Int.Biodeter.Biodegrad.,2014,86:248-251.
[20]田俊岭,彭桂香,李永涛,等.一种高效光合菌剂对辣椒生长及土壤微生物的影响[J].生物技术进展,2014,3:197-200.
[21]Gorlenko V M.History of the study of biodiversity of photosynthetic bacteria[J].Microbiology,2004,73(5):541-550.
[22]王馥迪,李征,黄秋生,等.光合细菌在植物上的应用现状及展望[J].广东蚕业,2012,2:36-38.
[23]谢修志,谢向坚.光合细菌菌剂对蔬菜品质影响的初报[J].广东农业科学,2009,12:86-87.
[24]史清亮,贺跃武,马玉珍,等.光合细菌在农业上的应用研究[J].山西农业科学,2000,28(2):59-62.
[25]中国科学院南京土壤研究所微生物室.土壤微生物研究法[M].北京:科学出版社,1985,54-57.
[26]张信娣,曹慧,徐冬青,等.光合细菌和有机肥对土壤主要微生物类群和土壤酶活性的影响[J].土壤,2008,40(3):443-447.
[27]张信娣,史永军,陈银科.光合细菌和有机肥对土壤主要微生物类群的影响[J].中国土壤与肥料,2007,3:59-62.
[28]杨芳,田俊岭,杨盼盼,等.高效光合细菌菌剂对番茄品质、土壤肥力及微生物特性的影响[J].华南农业大学学报,2014,35(1):49-54.
[29]何云辉.光合细菌的培养及其对土壤肥力的影响[D].成都:四川师范大学,硕士学位论文,2014,41-43.
[30]Gianfreda L,Sannino F,Vtoante A.Pesticide effects on the activity of free,immobilized and soil invertase[J].Soil Biol.Biochem.,1995,27(9):1201-1208.
[31]王桂芳.沼液配施钾肥对苹果园土壤酶活性和土壤微生物数量及果实品质的影响[D].陕西杨凌:西北农林科技大学,硕士学位论文,2009,14-16.
[32]蒲铃铃,刘桦,曾莉萍.光合细菌对土壤主要微生物类群的影响试验分析[J].生物技术世界,2012,4:11,27.
[33]白红娟,肖根林,贾万利,等.光合细菌提高污染土壤中酶活性的研究[J].工业安全与环保,2011,6:13-15.
[34]肖根林,白红娟,贾万利.光合细菌提高铅、镉及呋喃丹复合污染土壤中酶活性的研究[J].化工技术与开发,2011,2:47-50.
[35]王延军,宗良纲,李锐,等.不同肥料对有机栽培番茄生长和土壤酶及微生物量的影响[J].南京农业大学学报,2007,30(3):83-87.