污水厂尾水及受纳河流样品溶解有机物(DOM)的分子光谱解析及识别
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摘要
为了研究污水厂尾水对受纳水体的影响,采用XAD-8和阴阳离子交换树脂联用技术,对合肥市污水厂不同处理工艺出水以及朱砖井污水厂受纳河流上下游水样进行分离富集处理,获得了亲水性碱(HIB)、亲水性酸(HIA)、亲水性中性物质(HIN)、憎水性碱(HOB)、憎水性酸(HOA)和憎水性中性物质(HON) 6种组分。采用DOMfluor、PARAFAC、PAC投影等数学分析方法,将前述5种树脂分离组分以及原水水样的荧光光谱进行解析,获得类蛋白质、类富里酸和类胡敏酸3种主成分。结合溶解有机碳分析结果,得到DOM总有机物中憎水性有机质约占75%,主要为类富里酸和类胡敏酸等憎水性腐殖质。结合傅里叶变换红外光谱分析结果,发现亲水性物质主要包含多糖、蛋白质和磷酸盐化合物等物质,憎水性物质HOA、HOB主要包含脂类和有机酸等。通过比较二十埠河上下游水样以及树脂分离后组分的主成分得分值,发现纳污河流污染较重,污水厂尾水对受纳水体水质影响较小。
In order to investigate the influence of effluents from the sewage treatment plants to the receiving waters,XAD-8,anion-exchange and cation-exchange resins were used to separate and enrich the effluents from different sewage treatment plants and the samples from the upstream and downstream of the Zhu Zhuan Jing sewage treatment plant's outlet,and six components were acquired such as the hydrophilic base(HIB),hydrophilic acid(HIA),hydrophilic neutral substance(HIN),hydrophobic base(HOB),hydrophobic acid(HOA) and hydrophobic neutral substance(HON).Mathematical methods such as DOMFluor,PARAFAC and PAC projection approach were used to decompose the EEM fluorescene spectra of the former five components and those of the original effluents,and three principal components,i.e.the protein-like,fulvic-like and humic-like substances,were acquired.According to the analysis result of Fourier Tranform Infrared(FTIR) spectroscopy,hydrophilic substances were mainly composed by polysaccharides,proteins,phosphate,etc.On the other hand,hydrophobic substances were mainly composed by lipids,organic acids and so on.In addition,the principal components scores were compared which were decomposed from the EEM fluorescence spectra of the upstream and downstream samples from the Er Shi Bu river.Results show that the receiving waters was seriously polluted and the effluents from sewage treatment plants had little effect on the receiving waterbody.
In order to investigate the influence of effluents from the sewage treatment plants to the receiving waters,XAD-8,anion-exchange and cation-exchange resins were used to separate and enrich the effluents from different sewage treatment plants and the samples from the upstream and downstream of the Zhu Zhuan Jing sewage treatment plant's outlet,and six components were acquired such as the hydrophilic base(HIB),hydrophilic acid(HIA),hydrophilic neutral substance(HIN),hydrophobic base(HOB),hydrophobic acid(HOA) and hydrophobic neutral substance(HON).Mathematical methods such as DOMFluor,PARAFAC and PAC projection approach were used to decompose the EEM fluorescene spectra of the former five components and those of the original effluents,and three principal components,i.e.the protein-like,fulvic-like and humic-like substances,were acquired.According to the analysis result of Fourier Tranform Infrared(FTIR) spectroscopy,hydrophilic substances were mainly composed by polysaccharides,proteins,phosphate,etc.On the other hand,hydrophobic substances were mainly composed by lipids,organic acids and so on.In addition,the principal components scores were compared which were decomposed from the EEM fluorescence spectra of the upstream and downstream samples from the Er Shi Bu river.Results show that the receiving waters was seriously polluted and the effluents from sewage treatment plants had little effect on the receiving waterbody.
引文
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[15]付兵,欧娅,刘欢,等.Ba2+共掺杂YPO4:Tb3+荧光材料的水热合成与荧光性能[J].材料导报,2017,31(9):16-20.[FUBing,OU Ya,LIU Huan,et al.Ba2+Co-Doped YPO4:Tb3+Phosphors:Hydrothermal Synthesis and Photoluminescence Properties[J].Materials Review,2017,31(9):16-20.]
[16]戴晓虎,薛勇刚,刘华杰,等.基于固相萃取及高效液相色谱-荧光检测分析的污泥中氟喹诺酮类抗生素研究方法的开发[J].环境科学,2016(4):1553-1561.[DAI Xiao-hu,XUEYong-gang,LIU Hua-jie,et al.Development of Determination Method of Fluoroquinolone Antibiotics in Sludge Based on Solid Phase Extraction and HPLC-Fluorescence Detection Analysis[J].Environmental Science,2016(4):1553-1561.]
[17]XIAO K,SUN J Y,SHEN Y X,et al.Fluorescence Properties of Dissolved Organic Matter as a Function of Hydrophobicity and Molecular Weight:Case Studies From Two Membrane Bioreactors and an Oxidation Ditch[J].RSC Advances,2016,6(29):24050-24059.]
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[19]田雨,王学东,陈潇霖,等.土壤溶解性有机质荧光特征及其与铜的络合能力[J].环境科学,2016,37(6):2338-2344.[TIANYu,WANG Xue-dong,CHEN Xiao-lin,et al.Fluorescence Spectroscopic Haracteristics and Cu2+-Complexing Ability of Soil Dissolved Organic Matter[J].Environmental Science,2016,37(6):2338-2344.]
[20]卢松,江韬,张进忠,等.两个水库型湖泊中溶解性有机质三维荧光特征差异[J].中国环境科学,2015,35(2):516-523.[LUSong,JIANG Tao,ZHANG Jin-zhong,et al.Three-Dimensional Fluorescence Characteristic Differences of Dissolved Organic Matter(DOM)From Two Typical Reservoirs[J].China Environmental Science,2015,35(2):516-523.]
[21]汤景梅,梁淑轩,孙汉文,等.保定府河溶解性有机质三维荧光光谱分析[J].光谱学与光谱分析,2014,34(2):450-454.[TANG Jing-mei,LING Shu-xuan,SUN Han-wen,et al.Analysis of Dissolved Organic Matters in Fu River of Baoding Using Three Dimensional Fluorescence Excitation-Emission Matrix[J].Spectroscopy and Spectral Analysis,2014,34(2):450-454.]
[22]FELLMAN J B,D'AMORE D V,HOOD E,et al.Fluorescence Characteristics and Biodegradability of Dissolved Organic Matter in Forest and Wetland Soils From Coastal Temperate Watersheds in Southeast Alaska[J].Biogeochemistry,2008,88(2):169-184.
[23]李卫华,刘怡心,王伟,等.污水处理厂及受纳水体样品的三维荧光光谱解析[J].光谱学与光谱分析,2015,35(4):940-945.[LI Wei-hua,LIU Yi-xin,WANG Wei,et al.Analysis of Samples From Wastewater Treatment Plant and Receiving Waters Using EEM Fluorescence Spectroscopy[J].Spectroscopy and Spectral A-nalysis 2015,35(4):940-945.]
[24]金鹏康,孔茜,金鑫.二级出水中溶解性有机物的分级表征特性[J].环境化学,2015,34(9):1649-1653.[JIN Peng-kang,KONG Qian,JIN xin.Characterization of Dissolved Organic Matter in the Secondary Effluent of Urban Waste Water Treatment Plant[J].Environmental Chemistry,2015,34(9):1649-1653.]
[2]贾陈忠,孔淑琼,张彩香.溶解性有机物的特征及对环境污染物的影响[J].广州化工,2012,40(3):98-100.[JIA Chenzhong,KONG Shu-qiong,ZHANG Cai-xiang.Structural Characteristics of Dissolved Organic Matter and the Influence on Environmental Pollutants[J].Guangzhou Chemical Industry and Technology,2012,40(3):98-100.]
[3]高霞,周凌云,席景砖.荧光分析法在环境有机污染物检测中的应用[J].光谱实验室,2011,28(4):2008-2016.[GAO Xia,ZHOU Ling-Yun,XI Jing-Zhuan.Applications of Fluorescence A-nalysis Methods in Detection of Environmental Organic Pollutants[J].Chinese Journal of Spectroscopy Laboratory,2011,28(4):2008-2016.]
[4]WEI L L,WANG K,ZHAO Q L,et al.Kinetics and Equilibrium of Adsorption of Dissolved Organic Matter Fractions From Secondary Effluent by Fly Ash[J].Journal of Environmental Sciences,2011,23(7):1057-1065.
[5]SK I,BOYER T H.Behavior of Reoccurring PARAFACComponents in Fluorescent Dissolved Organic Matter in Natural and Engineered Systems:A Critical Review[J].Environmental Science&Technology,2012,46(4):2006-2017.
[6]HENDERSON R K,BAKER A,MURPHY K R,et al.Fluorescence as a Potential Monitoring Tool for Recycled Water Systems:A Review[J].Water Research,2009,43(4):863-881.
[7]MENG F,DREWS A,MEHREZ R,et al.Occurrence,Source,and Fate of Dissolved Organic Matter(DOM)in a Pilot-Scale Membrane Bioreactor[J].Environmental Science&Technology,2009,43(23):8821-8826.
[8]XUE S,ZHAO Q L,WEI L L,et al.Fluorescence Spectroscopic Studies of the Effect of Granular Activated Carbon Adsorption on Structural Properties of Dissolved Organic Matter Fractions[J].Frontiers of Environmental Science&Engineering,2012,6(6):784-796.
[9]洪志强,熊瑛,李艳,等.白洋淀沉水植物腐解释放溶解性有机物光谱特性[J].生态学报,2016,36(19):6308-6317.[HONGZhi-qiang,XIONG Ying,LI Yan,et al.The Spectra Characterization on Dissolved Organic Matter of Submerged Plant Decomposition in Lake Baiyangdian[J].Acta Ecologica Sinica,2016,36(19):6308-6317.]
[10]朱伟健,沈芳,洪官林.长江口及邻近海域有色溶解有机物(CDOM)的光学特性[J].环境科学,2010,31(10):2292-2298.[ZHU Wei-jian,SHEN Fang,HONG Guan-lin.Optical Characteristics of Colored Dissolved Organic Material(CDOM)in Yangtze Estuary[J].Environmental Science,2010,31(10):2292-2298.]
[11]金鑫,金鹏康,孔茜.基于PARAFAC分析的二级出水DOM臭氧化特性研究[J].中国环境科学,2015,35(2):427-433.[JIN Xin,JIN Peng-kang,KONG Qian,et al.Ozonation Characteristics of DOMin Secondary Effluent Based on Fluorescence PARAFAC Analysis[J].China Environmental Science,2015,35(2):427-433.]
[12]THURMAN E M.Organic Geochemistry of Natural Waters[M].Amsterdam,Holland:Springer,1985:103-109.
[13]王立英,吴丰昌,张润宇.应用XAD系列树脂分离和富集天然水体中溶解有机质的研究进展[J].地球与环境,2006,34(1):90-96.[WANG Li-ying,WU Feng-chang,ZHANG Run-yu.A Mathod of Separate and Concentrate Dissolved Organic Matter by XADResin in Natural Aquatic Systems[J].Earth and Environment,2006,34(1):90-96.]
[14]贺润升,徐荣华,韦朝海.焦化废水生物出水溶解性有机物特性光谱表征[J].环境化学,2015,34(1):129-136.[HE Run-sheng,XU Rong-hua,WEI Chao-hai.Spectral Characterization of Dissolved Organic Matter in Bio-Treated Effluent of Coking Wastewater[J].Environmental Chemistry,2015,34(1):129-136.]
[15]付兵,欧娅,刘欢,等.Ba2+共掺杂YPO4:Tb3+荧光材料的水热合成与荧光性能[J].材料导报,2017,31(9):16-20.[FUBing,OU Ya,LIU Huan,et al.Ba2+Co-Doped YPO4:Tb3+Phosphors:Hydrothermal Synthesis and Photoluminescence Properties[J].Materials Review,2017,31(9):16-20.]
[16]戴晓虎,薛勇刚,刘华杰,等.基于固相萃取及高效液相色谱-荧光检测分析的污泥中氟喹诺酮类抗生素研究方法的开发[J].环境科学,2016(4):1553-1561.[DAI Xiao-hu,XUEYong-gang,LIU Hua-jie,et al.Development of Determination Method of Fluoroquinolone Antibiotics in Sludge Based on Solid Phase Extraction and HPLC-Fluorescence Detection Analysis[J].Environmental Science,2016(4):1553-1561.]
[17]XIAO K,SUN J Y,SHEN Y X,et al.Fluorescence Properties of Dissolved Organic Matter as a Function of Hydrophobicity and Molecular Weight:Case Studies From Two Membrane Bioreactors and an Oxidation Ditch[J].RSC Advances,2016,6(29):24050-24059.]
[18]周珺,程海涛.水源水体溶解性有机物(DOM)三维荧光特性研究[J].科技创新导报,2011,8(32):1,3.
[19]田雨,王学东,陈潇霖,等.土壤溶解性有机质荧光特征及其与铜的络合能力[J].环境科学,2016,37(6):2338-2344.[TIANYu,WANG Xue-dong,CHEN Xiao-lin,et al.Fluorescence Spectroscopic Haracteristics and Cu2+-Complexing Ability of Soil Dissolved Organic Matter[J].Environmental Science,2016,37(6):2338-2344.]
[20]卢松,江韬,张进忠,等.两个水库型湖泊中溶解性有机质三维荧光特征差异[J].中国环境科学,2015,35(2):516-523.[LUSong,JIANG Tao,ZHANG Jin-zhong,et al.Three-Dimensional Fluorescence Characteristic Differences of Dissolved Organic Matter(DOM)From Two Typical Reservoirs[J].China Environmental Science,2015,35(2):516-523.]
[21]汤景梅,梁淑轩,孙汉文,等.保定府河溶解性有机质三维荧光光谱分析[J].光谱学与光谱分析,2014,34(2):450-454.[TANG Jing-mei,LING Shu-xuan,SUN Han-wen,et al.Analysis of Dissolved Organic Matters in Fu River of Baoding Using Three Dimensional Fluorescence Excitation-Emission Matrix[J].Spectroscopy and Spectral Analysis,2014,34(2):450-454.]
[22]FELLMAN J B,D'AMORE D V,HOOD E,et al.Fluorescence Characteristics and Biodegradability of Dissolved Organic Matter in Forest and Wetland Soils From Coastal Temperate Watersheds in Southeast Alaska[J].Biogeochemistry,2008,88(2):169-184.
[23]李卫华,刘怡心,王伟,等.污水处理厂及受纳水体样品的三维荧光光谱解析[J].光谱学与光谱分析,2015,35(4):940-945.[LI Wei-hua,LIU Yi-xin,WANG Wei,et al.Analysis of Samples From Wastewater Treatment Plant and Receiving Waters Using EEM Fluorescence Spectroscopy[J].Spectroscopy and Spectral A-nalysis 2015,35(4):940-945.]
[24]金鹏康,孔茜,金鑫.二级出水中溶解性有机物的分级表征特性[J].环境化学,2015,34(9):1649-1653.[JIN Peng-kang,KONG Qian,JIN xin.Characterization of Dissolved Organic Matter in the Secondary Effluent of Urban Waste Water Treatment Plant[J].Environmental Chemistry,2015,34(9):1649-1653.]