利用Illumina Miseq测序技术研究鲍内脏多肽对小鼠肠道微生物的影响
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摘要
[目的]研究鲍内脏活性物质对小鼠肠道微生物的影响。[方法]从鲍内脏中制备多肽并灌胃小鼠,利用Illumina Miseq测序技术分析小鼠肠道微生物的群落结构及动态变化。[结果]门水平上,小鼠肠道微生物最优势菌群为拟杆菌门(Bacteroidetes),占总序列的69.10%;其次为厚壁菌门(Firmicutes)和变形菌门(Proteobacteria),分别占总序列的23.50%和5.00%,变形菌门又以δ-变形菌纲居多。属水平上,最优势类群为拟杆菌目(Bacteroidales)的一个未知分类属,占30.33%;其次为拟普雷沃菌属(Alloprevotella)和拟杆菌属(Bacteroides),分别占12.47%和9.80%。灌胃鲍内脏多肽后,小鼠肠道中丰度前30位的菌属中有4个属的丰度上调,其中副拟杆菌属(Parabacteroides)的丰度显著上调。丰度下调的属有7个,其中毛螺菌科(Lachnospiraceae)一个未分类属和厌氧支原体属(Anaeroplasma)丰度显著下调。[结论]灌胃鲍内脏多肽后小鼠肠道微生物多样性呈增加的趋势,且在小鼠肠道中检测到一些与宿主健康相关的差异细菌类群。
[Objective] To study the effects of visceral active substance of abalone on intestinal microbe of mice by Illumina Miseq sequencing. [Method] Polypeptide extracted from abalone viscera and mice was fed with it by intragastric administration. The community structure and dynamic changes of intestinal microbe of mice were analyzed by using Illumina Miseq sequencing. [Result] The most dominant phyla of bacteria in the intestine of mice were Bacteroides, accounting for 69.10% of the total sequence, followed by Firmicutes and Proteobacteria( accounting for 23.50% and 5.00% of the total sequences, respectively). Proteobacteria was dominated by δ-Proteobacteria.At the genus level, the most dominant group was norank_f_Bacteroidales_S24-7_group, accounting for 30.33%, followed by Alloprevotella and Bacteroides(accounting for 12.47% and 9.80%, respectively). After the intragastric administration of abalone polypeptide, the abundance of 4 bacterial genera in the intestine of mice increased, among which the genus Parabcteroides significantly increased(P<0.05). The abundance of 7 genera decreased, amongwhich the genus norank_f_Lachnospiraceae and Anaeroplasma significantly decreased(P<0.05). [Conclusion] The intestinal microbial diversity of mice after intragastric administration of abalone viscera polypeptide, and some differentiated bacterial groups related to host health were detected in the intestinal tract of mice.
[Objective] To study the effects of visceral active substance of abalone on intestinal microbe of mice by Illumina Miseq sequencing. [Method] Polypeptide extracted from abalone viscera and mice was fed with it by intragastric administration. The community structure and dynamic changes of intestinal microbe of mice were analyzed by using Illumina Miseq sequencing. [Result] The most dominant phyla of bacteria in the intestine of mice were Bacteroides, accounting for 69.10% of the total sequence, followed by Firmicutes and Proteobacteria( accounting for 23.50% and 5.00% of the total sequences, respectively). Proteobacteria was dominated by δ-Proteobacteria.At the genus level, the most dominant group was norank_f_Bacteroidales_S24-7_group, accounting for 30.33%, followed by Alloprevotella and Bacteroides(accounting for 12.47% and 9.80%, respectively). After the intragastric administration of abalone polypeptide, the abundance of 4 bacterial genera in the intestine of mice increased, among which the genus Parabcteroides significantly increased(P<0.05). The abundance of 7 genera decreased, amongwhich the genus norank_f_Lachnospiraceae and Anaeroplasma significantly decreased(P<0.05). [Conclusion] The intestinal microbial diversity of mice after intragastric administration of abalone viscera polypeptide, and some differentiated bacterial groups related to host health were detected in the intestinal tract of mice.
引文
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[17] 许文琦,王生,黄逸安,等.非酒精性脂肪肝与酒精性肝病大鼠肠道菌群比较研究[J].世界临床药物,2018,39(1):35-42.
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[21] 陈鹏德,林燕,杨洁,等.过敏性紫癜患儿肠道菌群结构及多样性研究[J].中国妇幼健康研究,2017,28(7):747-752,757.
[22] 朱宏斌,沈伟,王竞,等.宏基因组研究高脂饮食诱导小鼠的肥胖易感性与肠道菌群的关系[J].第三军医大学学报,2017,39(8):773-780.
[2] 苗艳丽,方富永,宋文东.鲍鱼内脏的酸水解及其梅拉德反应产物分析[J].南方水产,2009,5(4):54-57.
[3] 林杰.PEF对鲍鱼内脏蛋白水解及其性质变化影响的研究[D].福州:福建农林大学,2012:1-2.
[4] 刘春燕.鲍鱼内脏多糖的结构和活性研究[D].长春:东北师范大学,2011:1-6.
[5] VILLAMIL O,VáQUIRO H,SOLANILLA J F.Fish viscera protein hydrolysates:Production,potential applications and functional and bioactive properties[J].Food Chem,2017,224:160-171.
[6] 王翠翠,刘明,王凤霞,等.文蛤多肽抑制肿瘤细胞微管蛋白聚合[J].中国生化药物杂志,2012,33(3):225-228.
[7] CAI L Y,WU X S,ZHANG Y H,et al.Purification and characterization of three antioxidant peptides from protein hydrolysate of grass carp(Ctenopharyngodon idella)skin[J].Journal of functional foods,2015,16(3):234-242.
[8] ZHOU D Y,ZHU B W,QIAO L,et al.In vitro antioxidant activity of enzymatic hydrolysates prepared from abalone(Haliotis discus hannai Ino)viscera[J].Food and bioproducts processing,2012,90(2):148-154.
[9] JE J Y,PARK S Y,HWANG J Y,et al.Amino acid composition and in vitro antioxidant and cytoprotective activity of abalone viscera hydrolysate[J].Journal of functional foods,2015,16:94-103.
[10] 何传波,邵杰,魏好程,等.鲍内脏蛋白肽抗氧化和免疫调节活性[J].食品科学,2018,39(5):206-212.
[11] SULERIA H A R,MASCI P P,ADDEPALLI R,et al.In vitro anti-thrombotic and anti-coagulant properties of blacklip abalone(Haliotis rubra)viscera hydrolysate[J].Analytical & bioanalytical chemistry,2017,409(17):4195-4205.
[12] O’HARA A M,SHANAHAN F.The gut flora as a forgotten organ[J].EMBO Reports,2006,7(7):688-693.
[13] B?CKHED F,LEY R E,SONNENBURG J L,et al.Host-bacterial mutualism in the human intestine[J].Science,2005,25(307):1915-1920.
[14] KAIKO G E,STAPPENBECK T S.Host-microbe interactions shaping the gastrointestinal environment[J].Trends in immunology,2014,35(11):538-548.
[15] 朱芳骞.鲍鱼内脏胶原蛋白多肽的制备及其体外抗氧化活性研究[D].福州:福建农林大学,2012:50-61.
[16] 杨雪.强化污泥厌氧发酵产酸的效能及发酵液碳源的利用研究[D].哈尔滨:哈尔滨工业大学,2012:72-77,133.
[17] 许文琦,王生,黄逸安,等.非酒精性脂肪肝与酒精性肝病大鼠肠道菌群比较研究[J].世界临床药物,2018,39(1):35-42.
[18] NASEER M I,BIBI F,ALQAHTANI M H,et al.Role of gut microbiota in obesity,type 2 diabetes and Alzheimer's disease[J].CNS & neurological disorders drug targets,2014,13(2):305-311.
[19] TAN A H,MAHADEVA S,THALHA A M,et al.Small intestinal bacterial overgrowth in Parkinson’s disease[J].Parkinsonism & related disorders,2014,20(5):535-540.
[20] 张静怡,唐颖悦,李春敏,等.非酒精性脂肪性肝病对肠道菌群多样性影响的初步研究[J].肝脏,2017,22(4):323-326.
[21] 陈鹏德,林燕,杨洁,等.过敏性紫癜患儿肠道菌群结构及多样性研究[J].中国妇幼健康研究,2017,28(7):747-752,757.
[22] 朱宏斌,沈伟,王竞,等.宏基因组研究高脂饮食诱导小鼠的肥胖易感性与肠道菌群的关系[J].第三军医大学学报,2017,39(8):773-780.