喜鹊雌雄个体血清蛋白比较分析
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摘要
采用聚丙烯酰胺凝胶垂直板电泳方法,对喜鹊(Pica pica)雌雄个体的血清蛋白进行分离分析。结果表明,喜鹊雌雄个体共分离出电泳迁移率0.852～0.031的20条谱带,雌、雄性个体分离出的谱带数分别是18条和17条,其中有15条谱带为雌雄个体的共有谱带。在迁移率0.316、0.063、0.054处的谱带为雌性个体特有的谱带,在迁移率0.406、0.391处的谱带为雄性个体特有的谱带。喜鹊雌雄个体血清蛋白的谱带分布和活性强弱存在明显差异。
The serum albumin of the male and female magpies(Pica pica) were isolated and analyzed by polyacrylamide gel vertical plate electrophoresis. The results showed that the male and female magpie individuals were isolated 20 bands withelectrophoretic mobility from 0.852～0.031, the females were isolated for 18 bands,and the males for 17 bands, among them15 bands were communal for the females and the males. The bands with the electrophoresis mobility 0.316, 0.063, 0.054 were unique for the females, and the bands with the electrophoresis mobility 0.406, 0.391 were unique for the males. There were significant differences in the band distribution and activity of serum albumin between the male and female magpies individuals.
The serum albumin of the male and female magpies(Pica pica) were isolated and analyzed by polyacrylamide gel vertical plate electrophoresis. The results showed that the male and female magpie individuals were isolated 20 bands withelectrophoretic mobility from 0.852～0.031, the females were isolated for 18 bands,and the males for 17 bands, among them15 bands were communal for the females and the males. The bands with the electrophoresis mobility 0.316, 0.063, 0.054 were unique for the females, and the bands with the electrophoresis mobility 0.406, 0.391 were unique for the males. There were significant differences in the band distribution and activity of serum albumin between the male and female magpies individuals.
引文
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[3]吴清江,桂建芳.鱼类遗传育种工程[M].上海:上海科学技术出版社,1999.
[4]杨贵强,徐绍刚,王建,等.几种鲑鳟鱼血清蛋白非变性聚丙烯酰胺凝胶电泳的研究[J].海洋与湖沼,2013,44(4):1069-1072.
[5]牛泽清.大鲵血清蛋白成分及其分子量的测定[J].山西中医学院学报,2000,1(2):31-33.
[6]张淑艳,王海英,王北宁.肝病患者血清蛋白电泳结果分析[J].中国误诊学杂志,2006,6(23):4516-4518.
[7]李雅娟,赵兴文,杨国成,等.黄颡鱼雌雄个体血清蛋白、酯酶同工酶及血红蛋白的电泳分析[J].吉林农业大学学报,2004,26(3):339-342.
[8]王梦芝,傅筑荫,王洪荣,等.矮脚黄鸡、兴义矮脚鸡血清蛋白多态性的比较研究[J].家畜生态学报,2007,28(5):31-34.
[9]秦玉刚,杨春文,金建丽,等.绿啄木鸟雌雄个体血清蛋白比较分析[J].黑龙江畜牧兽医,2014(12):124-125.
[10]张颖,孙大江,刘红柏.3种鲟血清蛋白的比较研究[J].大连水产学院学报,2006,21(3):283-286.
[11]张译元,唐红,郭延华,等.绵羊血清蛋白双向凝胶电泳技术的建立[J].新疆农业科学,2017,54(1):190-196.
[12]金建丽,杨春文,闫佳续,等.雀鹰雌雄个体血清蛋白的比较分析[J].贵州农业科学,2012,40(11):157-159.
[13]李建武,萧能庚,余瑞元,等.生物化学实验原理和方法[M].北京:北京大学出版社,2004.
[14]周国权,巫光宏,黄翠颜,等.聚丙烯酰胺凝胶电泳的快速脱色方法[J].植物生理学通讯,2006,42(1):95-97.
[15]杨宪孝,许玉德.戈壁红骆驼和白骆驼血清蛋白表型差异的研究[J].草食家畜,1993(5):35-37,54.
[16]马细兰,张勇,周立斌,等.脊椎动物雌雄生长差异的研究进展[J].动物学杂志,2009,44(2):141-146.