HPLC测定不同产地北豆根中蝙蝠葛碱含量
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摘要
目的建立不同产地北豆根中蝙蝠葛碱含量测定的HPLC方法。方法以C18为色谱柱填充剂,以乙腈-水-三乙胺(45∶55∶0.1)为流动相,检测波长为284 nm,流速为1.0 m L/min,柱温为30℃,建立8批不同来源的北豆根HPLC图。结果建立了检测北豆根的HPLC条件,在20~100μg/m L范围内,对照品进样量与峰面积呈良好的线性关系(r=0.999 5),蝙蝠葛碱的平均回收率为100.30%,RSD=1.000%。不同来源的北豆根中蝙蝠葛碱含量不同。结论本研究建立的HPLC方法灵敏,准确度、精密度、重复性好,操作简便,可作为北豆根中蝙蝠葛碱含量的检测方法。
Objective To establish an HPLC method to determine the contents of dauricine in Menispermi Rhizoma from different producing areas. Methods C18 was set as chromatographic column filler, with acetonitrilewater-triethylamine(45:55:0.1) as the mobile phase, 284 nm as the ultraviolet wavelength detection, 1 m L/min as the flow rate, 30 ℃ as the column temperature. HPLC chromatograms of eight different batches of Menispermi Rhizoma were established. Results HPLC testing conditions of Menispermi Rhizoma was established. Within 20–100 μg/m L, there was a good linear relationship between the injection volume of the reference substance and the peak area(r=0.999 5). The average recovery of dauricine was 100.30%, RSD=1.000%. The contents of dauricine in Menispermi Rhizoma from different producing areas were different. Conclusion The HPLC method is with sensitivity, accuracy, precision, good reproducibility and simple operation, which can be used as detection method to determine the content of dauricine in Menispermi Rhizoma.
Objective To establish an HPLC method to determine the contents of dauricine in Menispermi Rhizoma from different producing areas. Methods C18 was set as chromatographic column filler, with acetonitrilewater-triethylamine(45:55:0.1) as the mobile phase, 284 nm as the ultraviolet wavelength detection, 1 m L/min as the flow rate, 30 ℃ as the column temperature. HPLC chromatograms of eight different batches of Menispermi Rhizoma were established. Results HPLC testing conditions of Menispermi Rhizoma was established. Within 20–100 μg/m L, there was a good linear relationship between the injection volume of the reference substance and the peak area(r=0.999 5). The average recovery of dauricine was 100.30%, RSD=1.000%. The contents of dauricine in Menispermi Rhizoma from different producing areas were different. Conclusion The HPLC method is with sensitivity, accuracy, precision, good reproducibility and simple operation, which can be used as detection method to determine the content of dauricine in Menispermi Rhizoma.
引文
[1]国家药典委员会.中华人民共和国药典:第一增补本[M].北京:中国医药科技出版社,2012:附录94-95.
[2]郑艳春,秦婷,崔雅慧,等.北豆根化学成分及其药理作用的研究进展[J].中国医药导报,2011,8(13):9-10,33.
[3]刘威.中药材北豆根的质量与应用研究[D].沈阳:辽宁中医药大学,2006.
[4]刘威,张振秋,洪涛,等.HPLC法测定北豆根中蝙蝠葛碱和蝙蝠葛苏林碱的含量[J].中药材,2005,28(9):783-784.
[5]李井涛,李东飞.HPLC法测定不同产地北豆根中蝙蝠葛碱的含量[J].中国药事,2012,26(10):1122-1124.
[6]张艳,石玉生,王栋.高效液相色谱法测定北豆根精制总碱胶囊中蝙蝠葛碱含量[J].中医药信息,2008,25(3):69-70.
[7]朱孝芹,韩铁刚,张欢,等.北豆根提取物中有效成分分析方法的建立[J].时珍国医国药,2008,19(1):61-63.
[8]李广生,李慧勇,张清波,等.北豆根药材中蝙蝠葛碱和蝙蝠葛苏林碱的定量分析[J].中医药信息,2010,27(6):65-67.
[2]郑艳春,秦婷,崔雅慧,等.北豆根化学成分及其药理作用的研究进展[J].中国医药导报,2011,8(13):9-10,33.
[3]刘威.中药材北豆根的质量与应用研究[D].沈阳:辽宁中医药大学,2006.
[4]刘威,张振秋,洪涛,等.HPLC法测定北豆根中蝙蝠葛碱和蝙蝠葛苏林碱的含量[J].中药材,2005,28(9):783-784.
[5]李井涛,李东飞.HPLC法测定不同产地北豆根中蝙蝠葛碱的含量[J].中国药事,2012,26(10):1122-1124.
[6]张艳,石玉生,王栋.高效液相色谱法测定北豆根精制总碱胶囊中蝙蝠葛碱含量[J].中医药信息,2008,25(3):69-70.
[7]朱孝芹,韩铁刚,张欢,等.北豆根提取物中有效成分分析方法的建立[J].时珍国医国药,2008,19(1):61-63.
[8]李广生,李慧勇,张清波,等.北豆根药材中蝙蝠葛碱和蝙蝠葛苏林碱的定量分析[J].中医药信息,2010,27(6):65-67.