细胞粘附蛋白、HER-2、ZO-1和VEGF与弥漫型胃癌侵袭转移的相关性研究
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摘要
目的研究细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、紧密连接蛋白1 (zonula oc-cludens-1,ZO-1)和人表皮生长因子受体-2 (human epidermal growth factor receptor 2,HER-2)与胃癌侵袭转移的相关性研究。方法选取弥漫型胃癌患者60例,收集患者胃癌组织、癌旁组织(距癌2 cm)和远离胃癌的正常组织(距癌5 cm),采用Real-Time PCR和免疫组化法检测患者组织中ICAM-1、VEGF、ZO-1和HER-2 mRNA表达水平的表达。免疫组化法检测患者组织中ICAM-1、VEGF、ZO-1和HER-2蛋白表达水平。结果与对照组和癌旁组织ICAM-1、VEGF、ZO-1和HER-2 mRNA表达水平相比,胃癌组患者癌组织内ICAM-1、VEGF、ZO-1和HER-2 mRNA表达水平显著升高,差异具有统计学意义(P <0. 05)。免疫组织化学法结果显示,ICAM-1、VEGF、ZO-1和HER-2阳性表达和弥漫型胃癌的淋巴结转移、浸润深度和TNM分期具有明显的相关性,差异具有统计学意义(P <0. 05)。ICAM-1、VEGF、ZO-1和HER-2阳性表达和弥漫型胃癌患者的年龄、性别和肿瘤直径没有相关性,差异不具有统计学意义(P> 0. 05)。结论 ICAM-1、VEGF、ZO-1和HER-2与弥漫型胃癌的侵袭转移存在明显的相关性,可作为判断胃癌侵袭转移能力的有效指标。
Objective To study the effects of intercellular adhesion molecule-1( ICAM-1),vascular endothelial growth factor( endothelial) growth factor( VEGF),1( zonula oc-cludens-1( ZO-1) and human epidermal growth factor receptor 2( EGF receptor-2). The relationship between HER-2) and invasion and metastasis of gastric cancer. Methods 60 patients with diffuse gastric cancer admitted were selected and their gastric cancer tissues were collected. The expressions of ICAM-1 VEGFO-1 and HER-2 mRNA were detected by Real-Time PCR and immunohistochemistry in adjacent tissues( 2 cm from carcinoma) and normal tissues away from gastric cancer( 5 cm). The expression of ICAM-1 VEGF-1 and HER-2 protein was detected by immunohistochemical method. Results Compared with the control group and the adjacent tissues,the expression levels of ICAM-1 VEGFU ZO-1 and HER-2 mRNA in gastric cancer group were significantly higher than those in the control group( P < 0. 05). The expression levels of HER-2 mRNA and ICAM-1 VEGFU ZO-1 in gastric cancer tissue were significantly higher than those in the control group( P < 0. 05). The results of immunohistochemistry showed that the positive expression of ICAM-1 VEGFO-1 and HER-2 was significantly correlated with lymph node metastasis,depth of invasion and TNM stage of diffuse gastric carcinoma,and the difference was statistically significant( P < 0. 05). ICAM). The positive expression of ZO-1 and HER-2 was not correlated with age,sex and tumor diameter in patients with diffuse gastric cancer( P > 0. 05). Conclusion There is a significant correlation between the invasion and metastasis of diffuse gastric carcinoma and the expression of HER-2 and VEGFO-1,which can be used as an effective index to judge the ability of invasion and metastasis of gastric cancer.
Objective To study the effects of intercellular adhesion molecule-1( ICAM-1),vascular endothelial growth factor( endothelial) growth factor( VEGF),1( zonula oc-cludens-1( ZO-1) and human epidermal growth factor receptor 2( EGF receptor-2). The relationship between HER-2) and invasion and metastasis of gastric cancer. Methods 60 patients with diffuse gastric cancer admitted were selected and their gastric cancer tissues were collected. The expressions of ICAM-1 VEGFO-1 and HER-2 mRNA were detected by Real-Time PCR and immunohistochemistry in adjacent tissues( 2 cm from carcinoma) and normal tissues away from gastric cancer( 5 cm). The expression of ICAM-1 VEGF-1 and HER-2 protein was detected by immunohistochemical method. Results Compared with the control group and the adjacent tissues,the expression levels of ICAM-1 VEGFU ZO-1 and HER-2 mRNA in gastric cancer group were significantly higher than those in the control group( P < 0. 05). The expression levels of HER-2 mRNA and ICAM-1 VEGFU ZO-1 in gastric cancer tissue were significantly higher than those in the control group( P < 0. 05). The results of immunohistochemistry showed that the positive expression of ICAM-1 VEGFO-1 and HER-2 was significantly correlated with lymph node metastasis,depth of invasion and TNM stage of diffuse gastric carcinoma,and the difference was statistically significant( P < 0. 05). ICAM). The positive expression of ZO-1 and HER-2 was not correlated with age,sex and tumor diameter in patients with diffuse gastric cancer( P > 0. 05). Conclusion There is a significant correlation between the invasion and metastasis of diffuse gastric carcinoma and the expression of HER-2 and VEGFO-1,which can be used as an effective index to judge the ability of invasion and metastasis of gastric cancer.
引文
[1]白洁,惠明,梁灿灿.基质金属蛋白酶9及热休克蛋白60检测对早期胃癌的诊断价值[J].标记免疫分析与临床,2017,24(2):189-192.
[2]彭廷勇,何熙国,周琮凯.血清LXA4、G17及SF联合检测胃癌的临床诊断价值[J].标记免疫分析与临床,2017,24(9):1007-1010.
[3]许慈,蔡懿婷,董晴晴,等.胃癌RNA干扰抑制磷脂酰肌醇3-激酶p110β的表达增加氟尿嘧啶诱导人胃癌细胞凋亡的能力[J].解剖学研究,2016,38(1):1-6.
[4]张健,李瑞娟,张红艳,等.小分子化合物H-131抑制胃癌细胞增殖及机制[J].解剖学研究,2014,36(2):81-84.
[5]冯玉梅,李思颖.血清miR-101与miR-25在胃癌手术患者中的检测价值[J].检验医学与临床,2018,15(1):119-121.
[6]王丽,王浩,孟扬.右美托咪定对胃癌行外科治疗脆弱脑功能患者的术后影响[J].检验医学与临床,2017,14(19):2965-2966.
[7]徐鑫,贺恒鹏,刘勇.高脂血症患者血清黏附分子、超敏C反应蛋白、同型半胱氨酸的检测及其临床意义[J].现代医学,2014,42(1):38-41.
[8]黄珂,陈艳红.ICAM-1在儿童肿瘤中表达的临床意义[J].实用癌症杂志,2013,28(4):350-352.
[9]陈建永,徐虹,施琳琳,等.正肝汤对肝硬化大鼠肠道紧密连接蛋白OCLN和ZO-1表达的影响[J].中华中医药学刊,2015,33(12):2991-2994.
[10]贺李娴,谭钢.ZO-1/ZONAB信号通路与细胞的增殖分化[J].中国细胞生物学学报,2017,39(1):128-135.
[11]项涛,雷慧,谭绮琼,等.IL-27、IL-29及miRNA-497在HER-2阳性胃癌患者放射性粒子联合靶向治疗中的价值[J].重庆医学,2017,46(30):4204-4206.
[12]Urabe M,Ushiku T,Seto Y等.HER-2阳性胃癌对曲妥珠单抗为基础化疗的病理反应[J].临床与实验病理学杂志,2017,33(5):510.
[13]单玫,高守铭,叶玉莺.红芪多糖对链脲佐菌素诱导糖尿病模型大鼠视网膜VEGF与PEDF及CRP水平影响研究[J].四川中医,2017,35(11):54-57.
[14]巫朝伦,苗立减,宋青.桂枝茯苓汤加减联合穴位敷贴治疗寒凝血瘀型子宫肌腺症疗效及对Hb、VEGF、CA125水平的影响[J].四川中医,2017,35(9):132-135.
[2]彭廷勇,何熙国,周琮凯.血清LXA4、G17及SF联合检测胃癌的临床诊断价值[J].标记免疫分析与临床,2017,24(9):1007-1010.
[3]许慈,蔡懿婷,董晴晴,等.胃癌RNA干扰抑制磷脂酰肌醇3-激酶p110β的表达增加氟尿嘧啶诱导人胃癌细胞凋亡的能力[J].解剖学研究,2016,38(1):1-6.
[4]张健,李瑞娟,张红艳,等.小分子化合物H-131抑制胃癌细胞增殖及机制[J].解剖学研究,2014,36(2):81-84.
[5]冯玉梅,李思颖.血清miR-101与miR-25在胃癌手术患者中的检测价值[J].检验医学与临床,2018,15(1):119-121.
[6]王丽,王浩,孟扬.右美托咪定对胃癌行外科治疗脆弱脑功能患者的术后影响[J].检验医学与临床,2017,14(19):2965-2966.
[7]徐鑫,贺恒鹏,刘勇.高脂血症患者血清黏附分子、超敏C反应蛋白、同型半胱氨酸的检测及其临床意义[J].现代医学,2014,42(1):38-41.
[8]黄珂,陈艳红.ICAM-1在儿童肿瘤中表达的临床意义[J].实用癌症杂志,2013,28(4):350-352.
[9]陈建永,徐虹,施琳琳,等.正肝汤对肝硬化大鼠肠道紧密连接蛋白OCLN和ZO-1表达的影响[J].中华中医药学刊,2015,33(12):2991-2994.
[10]贺李娴,谭钢.ZO-1/ZONAB信号通路与细胞的增殖分化[J].中国细胞生物学学报,2017,39(1):128-135.
[11]项涛,雷慧,谭绮琼,等.IL-27、IL-29及miRNA-497在HER-2阳性胃癌患者放射性粒子联合靶向治疗中的价值[J].重庆医学,2017,46(30):4204-4206.
[12]Urabe M,Ushiku T,Seto Y等.HER-2阳性胃癌对曲妥珠单抗为基础化疗的病理反应[J].临床与实验病理学杂志,2017,33(5):510.
[13]单玫,高守铭,叶玉莺.红芪多糖对链脲佐菌素诱导糖尿病模型大鼠视网膜VEGF与PEDF及CRP水平影响研究[J].四川中医,2017,35(11):54-57.
[14]巫朝伦,苗立减,宋青.桂枝茯苓汤加减联合穴位敷贴治疗寒凝血瘀型子宫肌腺症疗效及对Hb、VEGF、CA125水平的影响[J].四川中医,2017,35(9):132-135.