2种脱水处理流程在病理切片制备中的应用及对比分析
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摘要
目的探讨肾穿刺标本手工及机器2种脱水处理方法在病理切片制备中的差异,总结出合适的脱水处理方法,从而制备规范化的肾穿刺病理切片,有助于提高肾脏病理诊断,为临床诊疗提供可靠的依据。方法送检肾穿刺标本分别进行手工及全自动封闭脱水机处理,处理后的标本分别进行苏木精-伊红染色(HE)及特殊染色。结果机器脱水与手工脱水处理相比,手工脱水处理的标本制备出的病理切片,颜色对比更鲜明、亮丽。结论优秀的病理切片有助于提高病理诊断。
Objective To investigate the difference between manual and mechanical dewatering of kidney puncture specimens in the preparation of pathological sections,and to summarize the appropriate dehydration treatment methods to prepare standardized pathological sections of renal puncture,which was helpful to improve the diagnosis of renal pathology clinical diagnosis and treatment to provide a reliable basis.Methods The renal puncture specimens were submitted for manual and automatic sealing dehydration treatment,the specimens were processed hematoxylin-eosin(HE)and special staining.Results Compared with the manual dehydration,the dehydration of the machine made the pathological sections of the samples which were dehydrated by hand,the color contrast was more vivid and bright.Conclusion Excellent pathological sections can help to improve the pathological diagnosis.
Objective To investigate the difference between manual and mechanical dewatering of kidney puncture specimens in the preparation of pathological sections,and to summarize the appropriate dehydration treatment methods to prepare standardized pathological sections of renal puncture,which was helpful to improve the diagnosis of renal pathology clinical diagnosis and treatment to provide a reliable basis.Methods The renal puncture specimens were submitted for manual and automatic sealing dehydration treatment,the specimens were processed hematoxylin-eosin(HE)and special staining.Results Compared with the manual dehydration,the dehydration of the machine made the pathological sections of the samples which were dehydrated by hand,the color contrast was more vivid and bright.Conclusion Excellent pathological sections can help to improve the pathological diagnosis.
引文
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[2]吴昊,阎红琳,成红豆,等.穿刺活检组织标准化处理方法[J].临床与实验病理学杂志,2016,32(7):827-828.
[3]王弦,江冬瑞,秦蓉.肾穿刺活检标本制片体会[J].临床与实验病理学杂志,2016,32(6):706-707.
[4]张绪斌,熊正文,张华东,等.骨组织脱钙技术及其应用[J].医学理论与实践,2011,24(11):1264-1265.
[5]邹万忠.肾活检病理学[M].3版.北京:北京大学医学出版社,2014:25-26.
[6]刘畅,杨琪,何志军.肾穿刺病理制片中特殊染色的问题及对策[J].诊断病理学杂志,2014,21(5):313-315.
[7]马恒辉,周晓军.组织切片常见问题与对策[J].临床与实验病理学杂志,2009,25(2):211-214.
[8]王玉芳,武彦,徐晓艳,等.病理组织切片制作不佳影响诊断的补救方法[J].山西医科大学学报,2013,44(6):496-497.
[9]王晓鸿,林宇静,郑燕璇,等.肾脏穿刺活检病理标本的制作与体会[J].中国实用医药,2009,4(29):195-196.
[10]梁英杰,凌启波,张威.临床病理学技术[M].北京:人民卫生出版社,2011:63.
[11]罗灿峤,莫木琼,聂钊铭,等.穿刺活检组织常规石蜡和常规冰冻HE制片的比较[J].山东医药,2014,54(41):22-24.
[12]苗月芬.穿刺活检病理诊断体会[J].实用医技杂志,2006,13(2):218-219.