液基薄层细胞学技术联合免疫荧光染色在尿足细胞检测中的应用
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摘要
目的观察液基薄层细胞学技术(TCT)联合间接免疫荧光染色对尿液中足细胞的染色效果并探讨其临床应用价值。方法收集50例2型糖尿病肾病患者和14例健康对照者晨尿标本,TCT制片与传统离心涂片后均行间接免疫荧光染色,观察足细胞形态。结果 64例标本中TCT制片满意率(85.94%)高于传统离心涂片(50.00%),TCT制片的足细胞检出率(73.47%)高于传统离心涂片(51.02%),差异有统计学意义(P<0.05)。2种方法检测足细胞均阳性时,TCT制片的阅片效果优于传统离心涂片。结论较传统离心涂片而言,TCT制片联合间接免疫荧光染色在尿足细胞检测中具有优越性。
Objective To compare the indirect immunofluorescence staining effect of urinary podocytes by thinprep liquid-based cytologic test(TCT) with that by the conventional centrifugal smear method, and explore its clinical application value. Methods The morning urine samples from 50 patients with type 2 diabetic nephropathy and 14 healthy controls were smeared with the TCT and conventional centrifugation method, respectively, and then the indirect immunofluorescence staining were performed to observe the morphology of podocytes. Results For 64 urine samples, the satisfactory rate of TCT smears(85.94%) was significantly higher than that of conventional smears(50.00%), and the podocyte detection rate of TCT smears(73.47%) was also significantly higher than that of conventional smears(51.02%)(P<0.05). When urinary podocytes of the same patient were positive by both methods, the reading effect of TCT smears was obviously superior to that of conventional smears. Conclusion The TCT combined with indirect immunofluorescence staining is obviously superior to the conventional centrifugal smear method in the podocyte diagnosis of urine samples.
Objective To compare the indirect immunofluorescence staining effect of urinary podocytes by thinprep liquid-based cytologic test(TCT) with that by the conventional centrifugal smear method, and explore its clinical application value. Methods The morning urine samples from 50 patients with type 2 diabetic nephropathy and 14 healthy controls were smeared with the TCT and conventional centrifugation method, respectively, and then the indirect immunofluorescence staining were performed to observe the morphology of podocytes. Results For 64 urine samples, the satisfactory rate of TCT smears(85.94%) was significantly higher than that of conventional smears(50.00%), and the podocyte detection rate of TCT smears(73.47%) was also significantly higher than that of conventional smears(51.02%)(P<0.05). When urinary podocytes of the same patient were positive by both methods, the reading effect of TCT smears was obviously superior to that of conventional smears. Conclusion The TCT combined with indirect immunofluorescence staining is obviously superior to the conventional centrifugal smear method in the podocyte diagnosis of urine samples.
引文
[1]张进生,渠渊,安红雷,等.尿液标本液基细胞薄层涂片检查在膀胱癌诊断中的应用[J].北京医学,2011,33(7):563-565.
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[7]Ikuma D, Hiromura K, Kajiyama H, et al. The correlation of urinary podocytes and podocalyxin with histological features of lupus nephritis[J]. Lupus, 2018, 27(3): 484-493.
[8]杨伏猛,刘倩,王彦,等.尿足细胞及其标志蛋白podocalyxin测定对2型糖尿病肾病的诊断价值[J].临床检验杂志,2016,34(5):378-381.
[9]Shen P, Shen J, Li W, et al.Urinary podocyte can be an indicator for the pathogenetic condition of patients with iga nephropathy [J].Clin Lab, 2014, 60(10): 1709-1715.
[10]Camici M. Urinary detection of podocyte injury[J].Biomed Pharmacother, 2007, 61(5): 245-249.
[11]Lee SY, Choi ME. Urinary biomarkers for early diabetic nephropathy: Beyond albuminuria[J].Pediatr Nephrol, 2015, 30(7): 1063-1075.
[12]Economou CG, Kitsiou PV, Tzinia AK, et al. Enhanced podocalyxin expression alters the structure of podocyte basal surface[J]. J Cell Sci, 2004, 117(Pt 15): 3281-3294.
[13]张春芝,白雪丽,孙新平,等.用免疫细胞化学染色法检测尿液中足细胞在肾小球疾病诊断中的初步应用[J].临床检验杂志,2013,31(6):428-429.
[14]李惊子,黄海长,刘颖,等.检测尿足细胞在活动性肾小球疾病中的意义[J].北京大学学报(医学版),2005,37(5):463-466.
[2]胡仁明.糖尿病肾病的诊断和防治——中国糖尿病肾病诊断和治疗的专家共识解读[J].糖尿病临床,2015,8(9):447-453.
[3]李建华,付志民,吴广平,等.液基细胞学检测对非妇科细胞标本肿瘤细胞诊断的意义[J].中国医科大学学报,2004,30(5):414-415,420.
[4]Ordon M, Boerner S, Zlotta AR, et al.The fate of an unsatisfactory urine cytology test among patients with urothelial carcinoma[J]. BJU Int, 2009, 104(11): 1641-1645.
[5]李红全,唐颛.TCT与传统涂片在尿脱落细胞学检测中的对比研究[J].河北医药,2014,40(20):3079-3081.
[6]Sun D, Zhao X, Meng L. Relationship between urinary podocytes and kidney diseases[J]. Ren Fail, 2012, 34(3): 403-407.
[7]Ikuma D, Hiromura K, Kajiyama H, et al. The correlation of urinary podocytes and podocalyxin with histological features of lupus nephritis[J]. Lupus, 2018, 27(3): 484-493.
[8]杨伏猛,刘倩,王彦,等.尿足细胞及其标志蛋白podocalyxin测定对2型糖尿病肾病的诊断价值[J].临床检验杂志,2016,34(5):378-381.
[9]Shen P, Shen J, Li W, et al.Urinary podocyte can be an indicator for the pathogenetic condition of patients with iga nephropathy [J].Clin Lab, 2014, 60(10): 1709-1715.
[10]Camici M. Urinary detection of podocyte injury[J].Biomed Pharmacother, 2007, 61(5): 245-249.
[11]Lee SY, Choi ME. Urinary biomarkers for early diabetic nephropathy: Beyond albuminuria[J].Pediatr Nephrol, 2015, 30(7): 1063-1075.
[12]Economou CG, Kitsiou PV, Tzinia AK, et al. Enhanced podocalyxin expression alters the structure of podocyte basal surface[J]. J Cell Sci, 2004, 117(Pt 15): 3281-3294.
[13]张春芝,白雪丽,孙新平,等.用免疫细胞化学染色法检测尿液中足细胞在肾小球疾病诊断中的初步应用[J].临床检验杂志,2013,31(6):428-429.
[14]李惊子,黄海长,刘颖,等.检测尿足细胞在活动性肾小球疾病中的意义[J].北京大学学报(医学版),2005,37(5):463-466.