黄芪生脉饮对模拟运输应激大鼠肺脏组织炎性细胞因子mRNA表达及NF-κB信号通路的影响
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摘要
为了探讨运输应激对动物肺脏免疫机能的影响及黄芪生脉饮的调控作用,本试验通过构建大鼠模拟运输应激模型,研究黄芪生脉饮对模拟运输应激大鼠体质量、皮质酮含量、肺组织结构影响,及肺脏组织IL-1β、IL-6、TNF-α和IFN-γmRNA表达和NF-κB信号通路的影响。将30只SD雄性大鼠随机分为对照组、模型组及黄芪生脉饮组,分别给黄芪生脉饮组大鼠连续灌服黄芪生脉饮,对照组、模型组大鼠灌服生理盐水7d后,将模型组和黄芪生脉饮组大鼠置于35℃、60r/min的水平摇床上每日摇晃2h,持续3d以构建模拟运输应激模型,于第3天结束后立即乙醚麻醉采集血清、肺组织样品进行检测。结果表明:模型组和黄芪生脉饮组大鼠体质量均显著低于对照组(P<0.05);黄芪生脉饮组大鼠血清皮质酮浓度显著低于模型组(P<0.05),但显著高于对照组(P<0.05);模拟运输应激可引起大鼠肺脏组织水肿,炎性细胞浸润,而黄芪生脉饮组大鼠肺组织结构炎性浸润、水肿程度均较模型组轻微;模型组大鼠的肺组织IL-1β、IL-6、TNF-α和IFN-’mRNA表达量均显著高于黄芪生脉饮组和对照组(P<0.05),且黄芪生脉饮组与对照组无明显差异(P>0.05);模型组大鼠肺脏组织IκB(和p65的磷酸化水平均显著高于对照组和黄芪生脉饮组(P<0.05),而黄芪生脉饮组与对照组无显著性差异(P>0.05)。因此,模拟运输应激可诱导大鼠肺组织的炎性损伤,黄芪生脉饮可通过NF-κB信号通路抑制大鼠肺组织的炎性细胞因子表达,缓解大鼠模拟运输应激性肺脏组织炎性损伤。
In order to investigate the effects of Huangqi Shengmai decoction on immune function in lung tissue in rat injured with mimic transport stress,using the model of transport stress rat studed the effects of Huangqi Shengmai decoction on the body quality,CORT concentration in serum,pathological structure,and even the IL-1β,IL-6,TNF-α,IFN-' mRNA expression and the effect on the NF-κB signal pathway.30 SD rats were feed in normal conditions for 7 days,then were divided into control group,model group and HQSMY group.Before transport stress,the rats of HQSMY group were administered with Huangqi Shengmai decoction,and those of control and model group were administered with normal saline via gavage daily for 7 days,then the rats of model and HQSMY groups were placed in horizontal shaker shakingat 60 r/min,35℃for 3 consecutive days,to construct the simulated transport stress.The serum and lung tissue sample in rats anesthetized with diethylether were collected after shaking 3 days.The results showed that the stimulated transport stress could decrease the body quality of rats in model and HQSMY group in significantly(P<0.05),and the CORT concentration of HQSMY group was lower than that of model group,but higher than the that of control group significantly(P<0.05),the simulated transport stress could cause edema and inflammatory cell infiltrate of lung in rats,while the pathological changes of rats in HQSMY group were mild.The IL-1β,IL-6,TNF-α,IFN-' mRNA expression,IκB(and p65 phosphorylation in model group were significantly higher than that of control and HQSMY group(P<0.05),and there were not significantly between the two groups.Therefore,the simulated transport stress could cause the inflammatory damage in lung,while the Huangqi Shengmai decoction could depress the inflammation cytokines expression via NF-κB signal pathway,then healing the lung injury in rat with transport stress.
In order to investigate the effects of Huangqi Shengmai decoction on immune function in lung tissue in rat injured with mimic transport stress,using the model of transport stress rat studed the effects of Huangqi Shengmai decoction on the body quality,CORT concentration in serum,pathological structure,and even the IL-1β,IL-6,TNF-α,IFN-' mRNA expression and the effect on the NF-κB signal pathway.30 SD rats were feed in normal conditions for 7 days,then were divided into control group,model group and HQSMY group.Before transport stress,the rats of HQSMY group were administered with Huangqi Shengmai decoction,and those of control and model group were administered with normal saline via gavage daily for 7 days,then the rats of model and HQSMY groups were placed in horizontal shaker shakingat 60 r/min,35℃for 3 consecutive days,to construct the simulated transport stress.The serum and lung tissue sample in rats anesthetized with diethylether were collected after shaking 3 days.The results showed that the stimulated transport stress could decrease the body quality of rats in model and HQSMY group in significantly(P<0.05),and the CORT concentration of HQSMY group was lower than that of model group,but higher than the that of control group significantly(P<0.05),the simulated transport stress could cause edema and inflammatory cell infiltrate of lung in rats,while the pathological changes of rats in HQSMY group were mild.The IL-1β,IL-6,TNF-α,IFN-' mRNA expression,IκB(and p65 phosphorylation in model group were significantly higher than that of control and HQSMY group(P<0.05),and there were not significantly between the two groups.Therefore,the simulated transport stress could cause the inflammatory damage in lung,while the Huangqi Shengmai decoction could depress the inflammation cytokines expression via NF-κB signal pathway,then healing the lung injury in rat with transport stress.
引文
[1]MINKA N S,AYO J O.Physiological responses of food animals to road transportation stress[J].Afr J Biotechnol,2009,8(25):7415-7427.
[2]裴志花,王开,马红霞,等.运输应激及其对动物免疫功能影响的研究进展[J].中国兽医杂志,2012,48(1):81-83.
[3]王天奇,白喜婷,董发明.运输应激导致猪胸膜肺炎的诊断和控制[J].黑龙江畜牧兽医,2004(6):44.
[4]杜永学,夏迎华.牛运输应激呼吸综合征的防治[J].今日畜牧兽医,2013(12):56-56.
[5]杨恒怀.家畜应激综合症的辨证论治[J].中兽医医药杂志,2001(1):23-25.
[6]刘燕娟,王飞雪,郝建峰,等.黄芪生脉饮临床应用及基础研究进展[J].现代中西医结合杂志,2009,18(16):1961-1963
[7]Allen I C,Wilson J E,Schneider M,et al.NLRP12suppresses colon inflammation and tumorigenesis through the negative regulation of noncanonical NF-κB signaling[J].Immunity,2012,36(5):742-754.
[8]Wan C,Chen Y,Yin P,et al.Transport stress induces apoptosis in rat myocardial tissue via activation of the mitogen-activated protein kinase signaling pathways[J].Heart Vessels,2016,31(2):212-221.
[9]张林,张海军,岳洪源,等.运输应激对畜禽的影响及其应对措施[J].家畜生态学报,2009,30(2):106-109.
[10]李玉保,孙培明,王志亮,等.运输应激猪组织病理性损伤、HSP27的组织分布及其相关性研究[J].南京农业大学学报,2005,28(3):83-87.
[11]俞明远,王利利,马彤辉,等.运输应激对大鼠行为、血清皮质酮及下丘脑视上核nNOS表达的影响[J].中国农业大学学报,2016,21(1):81-86.
[12]汤晓晨,俞峰,孙书龙,等.黄芪甲苷对人膝骨关节炎退变关节软骨IL-1#表达的影响[J].南京中医药大学学报,2013(1):48-52.
[13]李洁,彭丽,王杨天,等.五味子及复方中药对实验性慢性心理应激下水上漂浮并高强度运动大鼠IL-1、IL-2、IL-6影响的研究[J].实用预防医学,2011(1):14-16.
[14]杨汉春.动物免疫学[M].2版.北京:中国农业大学出版社,2003:96-99.
[15]郭甫坤,吴曙光.白介素1信号转导研究进展[J].国外医学(分子生物学分册),2001(1):13-15.
[16]白雪,赵建增,梁志选,等.IFN-γ研究进展及其在猪繁殖与呼吸综合征研究中的应用[J].中国畜牧兽医,2010,12:145-150.
[17]韩瑾瑾,贺丛,吕国栋,等.肉牛运输应激综合征的诊治[J].畜牧与饲料科学,2010,31(5):179-181.
[18]SALES M,ATA M,WILLIAMSON B,et al.Effects of genotype and transportation stress on cytokine gene expression in steers[J].Ani Sci Arkansas,2014:69.
[19]LYU Q,ZHANG S,ZHAO R.Transportation stress alters the expression of immunoregulatory cytokines in the porcine thymus[J].Vet J,2011,187(2):229-233.
[20]孟昭琴,司绪岚,房冬梅.游泳和五味子多糖对CFS大鼠的干预及IFN-γ、IL-4等指标的影响[J].江苏师范大学学报(自然科学版),2012(3):73-78.
[21]李静,明亮,黄茸茸,等.黄芪提取物对大鼠全脑缺血再灌注后IL-1#、TNF-(和IL-6表达的影响[J].安徽医科大学学报,2005(6):26-28.
[22]刘萍,张静生.黄芪复方对EAMG大鼠血清IL-4、IFN-’、TGF-#对水平的影响研究[J].中医药学刊,2004(9):1611-1612+1629.
[23]杨晓航,史传道,叶峥嵘,等.黄芪总黄酮对佐剂性关节炎模型大鼠IL-4、IFN-γmRNA表达影响的研究[J].陕西医学杂志,2010(8):944-946+949.
[24]HRISTIAN F,SMITH E L,CARMODY R J.The regulation of NF-κB subunits by phosphorylation[J].Cells,2016,5(1):1-19
[25]BEN-MARIAH Y,KARIN M.Inflammation meets cancer,with NF-[kappa]B as the matchmaker[J].Nature Immunol,2011,12(8):715-723.
[26]CHEN Y W.Transport stress induces apoptosis in rat myocardial tissue via activation of the mitogen-activated protein kinase signaling pathways[J].HeartκVessels,2014,25(3):1-10.
[27]夏进,徐臻.黄芪生脉饮佐治病毒性心肌炎的临床效果观察[J].中医临床研究,2015(27):79-80.
[2]裴志花,王开,马红霞,等.运输应激及其对动物免疫功能影响的研究进展[J].中国兽医杂志,2012,48(1):81-83.
[3]王天奇,白喜婷,董发明.运输应激导致猪胸膜肺炎的诊断和控制[J].黑龙江畜牧兽医,2004(6):44.
[4]杜永学,夏迎华.牛运输应激呼吸综合征的防治[J].今日畜牧兽医,2013(12):56-56.
[5]杨恒怀.家畜应激综合症的辨证论治[J].中兽医医药杂志,2001(1):23-25.
[6]刘燕娟,王飞雪,郝建峰,等.黄芪生脉饮临床应用及基础研究进展[J].现代中西医结合杂志,2009,18(16):1961-1963
[7]Allen I C,Wilson J E,Schneider M,et al.NLRP12suppresses colon inflammation and tumorigenesis through the negative regulation of noncanonical NF-κB signaling[J].Immunity,2012,36(5):742-754.
[8]Wan C,Chen Y,Yin P,et al.Transport stress induces apoptosis in rat myocardial tissue via activation of the mitogen-activated protein kinase signaling pathways[J].Heart Vessels,2016,31(2):212-221.
[9]张林,张海军,岳洪源,等.运输应激对畜禽的影响及其应对措施[J].家畜生态学报,2009,30(2):106-109.
[10]李玉保,孙培明,王志亮,等.运输应激猪组织病理性损伤、HSP27的组织分布及其相关性研究[J].南京农业大学学报,2005,28(3):83-87.
[11]俞明远,王利利,马彤辉,等.运输应激对大鼠行为、血清皮质酮及下丘脑视上核nNOS表达的影响[J].中国农业大学学报,2016,21(1):81-86.
[12]汤晓晨,俞峰,孙书龙,等.黄芪甲苷对人膝骨关节炎退变关节软骨IL-1#表达的影响[J].南京中医药大学学报,2013(1):48-52.
[13]李洁,彭丽,王杨天,等.五味子及复方中药对实验性慢性心理应激下水上漂浮并高强度运动大鼠IL-1、IL-2、IL-6影响的研究[J].实用预防医学,2011(1):14-16.
[14]杨汉春.动物免疫学[M].2版.北京:中国农业大学出版社,2003:96-99.
[15]郭甫坤,吴曙光.白介素1信号转导研究进展[J].国外医学(分子生物学分册),2001(1):13-15.
[16]白雪,赵建增,梁志选,等.IFN-γ研究进展及其在猪繁殖与呼吸综合征研究中的应用[J].中国畜牧兽医,2010,12:145-150.
[17]韩瑾瑾,贺丛,吕国栋,等.肉牛运输应激综合征的诊治[J].畜牧与饲料科学,2010,31(5):179-181.
[18]SALES M,ATA M,WILLIAMSON B,et al.Effects of genotype and transportation stress on cytokine gene expression in steers[J].Ani Sci Arkansas,2014:69.
[19]LYU Q,ZHANG S,ZHAO R.Transportation stress alters the expression of immunoregulatory cytokines in the porcine thymus[J].Vet J,2011,187(2):229-233.
[20]孟昭琴,司绪岚,房冬梅.游泳和五味子多糖对CFS大鼠的干预及IFN-γ、IL-4等指标的影响[J].江苏师范大学学报(自然科学版),2012(3):73-78.
[21]李静,明亮,黄茸茸,等.黄芪提取物对大鼠全脑缺血再灌注后IL-1#、TNF-(和IL-6表达的影响[J].安徽医科大学学报,2005(6):26-28.
[22]刘萍,张静生.黄芪复方对EAMG大鼠血清IL-4、IFN-’、TGF-#对水平的影响研究[J].中医药学刊,2004(9):1611-1612+1629.
[23]杨晓航,史传道,叶峥嵘,等.黄芪总黄酮对佐剂性关节炎模型大鼠IL-4、IFN-γmRNA表达影响的研究[J].陕西医学杂志,2010(8):944-946+949.
[24]HRISTIAN F,SMITH E L,CARMODY R J.The regulation of NF-κB subunits by phosphorylation[J].Cells,2016,5(1):1-19
[25]BEN-MARIAH Y,KARIN M.Inflammation meets cancer,with NF-[kappa]B as the matchmaker[J].Nature Immunol,2011,12(8):715-723.
[26]CHEN Y W.Transport stress induces apoptosis in rat myocardial tissue via activation of the mitogen-activated protein kinase signaling pathways[J].HeartκVessels,2014,25(3):1-10.
[27]夏进,徐臻.黄芪生脉饮佐治病毒性心肌炎的临床效果观察[J].中医临床研究,2015(27):79-80.