盐碱胁迫对两种铁线莲生长状况和生理特性的影响
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摘要
以3年生褐毛铁线莲(Clematis fusc)和齿叶铁线莲(Clematis serratifolia)为研究对象,研究不同盐碱胁迫条件对两种铁线莲生长和生理指标的影响。结果表明:在低浓度的盐碱胁迫条件下,齿叶铁线莲和褐毛铁线莲生长发育未受较大影响;中高浓度盐碱胁迫下,齿叶铁线莲植株大部分叶片脱色发黄,褐毛铁线莲有部分植株出现死亡现象;两种铁线莲的叶绿素质量分数均随着盐碱溶液的浓度和胁迫时间的增加而减小,丙二醛质量摩尔浓度和脯氨酸质量分数均随着盐溶液浓度和胁迫时间的增加有增加的趋势;盐碱胁迫下,齿叶铁线莲的SOD、POD活性随处理时间的延长而增强,褐毛铁线莲SOD、POD活性在低浓度胁迫下随时间的增加活性增强,在高浓度处理时随时间的增加有先升高后降低的趋势。齿叶铁线莲和褐毛铁线莲对低浓度的盐碱均有一定的抗性,齿叶铁线莲较褐毛铁线莲具有更强的盐碱抗性。
Triennial Clematis fusc and Clematis serratifolia were used to investigate their growth and physiological response to the effects of different saline-alkali stress conditions. Under low salt and alkali stresses,the growth and development of two species of Clematis were not affected significantly. Under the moderate or severe saline-alkali stress conditions,most leaves turn wilting and yellow,C. serratifolia died. The chlorophyll quality score of two species of Clematis showed a tend of falling with the salt concentration and stress time rising,and the index of malondialdehyde and proline showed a trend of rising with the increase of salt solution rising. The activities of SOD and POD showed a trend of rising with the prolongation of treatment time under saline-alkali stress. The activities of C. serratifolia SOD and POD showed a trend of rising first under low content,and under high concentration it showed the trend of increase firstly and then decrease in high concentration.Two Clematis species showed a certain degree of resistance during the treatment of low saline-alkali content,but C. serratifolia showed stronger saline-alkali resistance.
Triennial Clematis fusc and Clematis serratifolia were used to investigate their growth and physiological response to the effects of different saline-alkali stress conditions. Under low salt and alkali stresses,the growth and development of two species of Clematis were not affected significantly. Under the moderate or severe saline-alkali stress conditions,most leaves turn wilting and yellow,C. serratifolia died. The chlorophyll quality score of two species of Clematis showed a tend of falling with the salt concentration and stress time rising,and the index of malondialdehyde and proline showed a trend of rising with the increase of salt solution rising. The activities of SOD and POD showed a trend of rising with the prolongation of treatment time under saline-alkali stress. The activities of C. serratifolia SOD and POD showed a trend of rising first under low content,and under high concentration it showed the trend of increase firstly and then decrease in high concentration.Two Clematis species showed a certain degree of resistance during the treatment of low saline-alkali content,but C. serratifolia showed stronger saline-alkali resistance.
引文
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[27]何淼,王欢,徐鹏飞,等.模拟复合盐碱胁迫对芒幼苗生理特性的影响[J].草业科学,2016,33(7):1342-1352.
[28]陈涛,王贵美,沈伟伟,等.盐胁迫对红麻幼苗生长及抗氧化酶活性的影响[J].植物科学学报,2011,29(4):493-501.
[29]陈展宇,常雨婷,邓川,等.盐碱生境对甜高粱幼苗抗氧化酶活性和生物量的影响[J].吉林农业大学学报,2017,39(1):15-19.
[30]陈莉.复合盐胁迫对小麦幼苗生理指标的影响[J].贵州农业科学,2011,39(1):56-58.
[31]原红娟,陈丽.复合盐、Mg SO4胁迫下三种叶菜幼苗生理特性的影响[J].北方园艺,2011(7):11-15.
[2]王文采,李良千.铁线莲属:新分类系统[J].植物分类学报,2005,43(5):431-488.
[3]张鸽香,武珊珊.我国铁线莲属植物的研究现状及其园林应用方式[J].安徽农业科学,2010,38(22):12076-12078.
[4]孙震.十种野生地被植物生态适应性的研究[D].北京:北京林业大学,2006.
[5]吴红,李成忠,张成霞.钝齿铁线莲根尖组培再生体系的研究[J].云南农业大学学报(自然科学),2017,32(1):120-124.
[6]朱玉雪,张敏涛,刘莹,等.4种早花大花组铁线莲的耐热性综合评价[J].上海交通大学学报(农业科学版),2017,35(1):58-65,71.
[7]刘凯月,曹宇,刘冬云.干旱胁迫对大叶铁线莲形态及生理指标的影响[J].河北林业科技,2017(1):1-4.
[8]王非,王凯,王竞红.遮阴对2种铁线莲光合特性的影响[J].东北林业大学学报,2017,45(2):12-16.
[9]王非,王金侠,李强,等.GA3和IAA处理对4种铁线莲种子萌发的影响[J].草业科学,2014,31(4):672-676.
[10]张兴,王云云,孙力,等.四种野生宿根花卉耐盐碱性分析[J].黑龙江科学,2015,6(6):4-5,19.
[11]岳桦,姜丽颖,吴妍.Na2CO3、Na HCO3胁迫对大叶铁线莲生理特性的影响[J].东北林业大学学报,2011,39(3):28-30.
[12]郭瑞,李峰,周际,等.亚麻响应盐、碱胁迫的生理特征[J].植物生态学报,2016,40(1):69-79.
[13]CAPULA-RODRLGUEZ R,VALDEZ-AGUILARA L A,CARTMILL D L,et al.Supplementary calcium and potassium improve the response of tomato(Solanum lycopersicum L.)to simultaneous alkalinity,salinity,and boron stress[J].Communications in Soil Science and Plant Analysis,2016,47(4):505-511.
[14]潘多锋,申忠宝,王建丽,等.碱性盐胁迫对偃麦草苗期生长的影响[J].草业科学,2016,33(11):2276-2282.
[15]李子英,丛日春,杨庆山,等.盐碱胁迫对柳树幼苗生长和渗透调节物质含量的影响[J].生态学报,2017,37(24):8511-8517.
[16]纪荣花,于磊,鲁为华,等.盐碱胁迫对芨芨草种子萌发的影响[J].草业科学,2011,28(2):245-250.
[17]郎志红.盐碱胁迫对植物种子萌发和幼苗生长的影响[D].兰州:兰州交通大学,2008.
[18]李合生.现代植物生理学[M].北京:高等教育出版社,2012:129-137.
[19]张丽,徐志然,胡晓辉,等.叶面喷施亚精胺对盐碱胁迫下番茄幼苗生长及其叶绿素合成前体含量的影响[J].西北植物学报,2015,35(1):125-130.
[20]KALAJI H M,JAJOO A,OUKARROUM A,et al.Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions[J].Acta Physiologiae Plant,2016,38:102.
[21]刘卫国,丁俊祥,邹杰,等.Na Cl对齿肋赤藓叶肉细胞超微结构的影响[J].生态学报,2016,36(12):3556-3563.
[22]CHENG T L,CHEN J H,ZHANG J B,et al.Physiological and proteomic analyses of leaves from the halo phyte Tangut Nitratia reveals diverse response pathways critical for high salinity tolerance[J].Front Plant Science,2015,6(30):30.
[23]ELSTNER E F.Oxygen activation and oxygen toxicity[J].Annual Review of Plant Physiology,1982,33(1):73-96.
[24]郑世英,商学芳,王景平.可见分光光度法测定盐胁迫下玉米幼苗抗氧化酶活性及丙二醛质量摩尔浓度[J].生物技术通报,2010(7):106-109.
[25]费伟,陈火英,曹忠,等.盐胁迫对番茄幼苗生理特性的影响[J].上海交通大学学报(农业科学版),2005,23(1):5-9,30.
[26]於丙军,章文华,刘友良.Na Cl对大麦幼苗根系蛋白质和游离氨基酸含量的影响[J].西北植物学报,1997,17(4):439-445.
[27]何淼,王欢,徐鹏飞,等.模拟复合盐碱胁迫对芒幼苗生理特性的影响[J].草业科学,2016,33(7):1342-1352.
[28]陈涛,王贵美,沈伟伟,等.盐胁迫对红麻幼苗生长及抗氧化酶活性的影响[J].植物科学学报,2011,29(4):493-501.
[29]陈展宇,常雨婷,邓川,等.盐碱生境对甜高粱幼苗抗氧化酶活性和生物量的影响[J].吉林农业大学学报,2017,39(1):15-19.
[30]陈莉.复合盐胁迫对小麦幼苗生理指标的影响[J].贵州农业科学,2011,39(1):56-58.
[31]原红娟,陈丽.复合盐、Mg SO4胁迫下三种叶菜幼苗生理特性的影响[J].北方园艺,2011(7):11-15.
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