灵芝蛛网病病原菌及其生物学特性
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摘要
为明确吉林省蛟河市灵芝Ganoderma lingzhi栽培主产区发生的疑似灵芝蛛网病的病原菌,作者通过罹病灵芝子实体病原物的分离纯化、致病性测定、形态学和分子生物学鉴定以及病原菌的生物学特性研究,证明引起吉林省蛟河市灵芝蛛网病的病原菌为嗜菌枝葡霉Cladobotryum mycophilum。该菌营养体最适生长条件为温度25°C、pH 5、蔗糖作碳源、酵母浸粉作氮源,光照对菌丝体生长有一定的抑制作用,完全黑暗最适宜生长。本文研究结果为进一步研究该病害的发生规律和防治措施提供了理论参考。
In 2018, the alleged cobweb disease was found in Ganoderma lingzhi-growing farm at Jiaohe, Jilin Province. Morphological identification and molecular analysis of ITS DNA sequence proved that the pathogen was Cladobotryum mycophilum. Artificial inoculation confirmed that the pathogen infected fruitbodies of Ganoderma lingzhi. Its mycelia grew favorably under the conditions of 25℃ and pH 5 on medium using glucose as carbon source and yeast extract as nitrogen source. Light might inhibit mycelial growth to some extent,while dark stimulated mycelial growth. The results provide a useful reference for effective control of the cobweb disease.
In 2018, the alleged cobweb disease was found in Ganoderma lingzhi-growing farm at Jiaohe, Jilin Province. Morphological identification and molecular analysis of ITS DNA sequence proved that the pathogen was Cladobotryum mycophilum. Artificial inoculation confirmed that the pathogen infected fruitbodies of Ganoderma lingzhi. Its mycelia grew favorably under the conditions of 25℃ and pH 5 on medium using glucose as carbon source and yeast extract as nitrogen source. Light might inhibit mycelial growth to some extent,while dark stimulated mycelial growth. The results provide a useful reference for effective control of the cobweb disease.
引文
Back CG,Kim YH,Jo WS,Chung H,Jung HY,2010.Cobweb disease on Agaricus bisporus caused by Cladobotryum mycophilum in Korea.Journal of General Plant Pathology,76:232-235
Back CG,Lee CY,Seo GS,Jung HY,2012.Characterization of species of Cladobotryum which cause cobweb disease in edible mushrooms grown in Korea.Mycobiology,40:189-194
Cai WM,Fang JL,Fan LF,Wei HH,1993.Identification and biological characters of the fungal pathogens of the soft rot of Flammulina velutipes.Acta Agriculturae Zhejiangensis,5(2):99-103(in Chinese)
Cao NN,Wang YN,2015.Insect pests and diseases in the cultivation of Ganoderma lucidum in Lantian county.Edible Fungi,37(6):56-57(in Chinese)
Cao Y,Wu SH,Dai YC,2012.Species clarification of the prize medicinal Ganoderma mushroom“Lingzhi”.Fungal Diversity,56:49-62
Carrasco J,Navarro MJ,Gea FJ,2017.Cobweb,a serious pathology in mushroom crops:a review.Spanish Journal of Agricultural Research,15(2):e10R01
Chang GB,Chang YL,Geon SS,Hee YJ,2012.First occurrence of cobweb disease on Hypsizigus marmoreus caused by Cladobotryum varium in Korea.Journal of the Faculty of Agriculture Kyushu University,57(2):373-377
Dai YC,Cao Y,Zhou LW,Wu SH,2013.Notes on the nomenclature of the most widely cultivated Ganoderma species in China.Mycosystema,32:947-952(in Chinese)
Fang ZD,1998.Methodology on plant pathology research.3rd ed.China Agricultural Press,Beijing.122-145(in Chinese)
Fu YP,Wang XX,Li D,Liu Y,Song B,Zhang CL,Wang Q,Chen MY,Zhang ZW,Li Y,2016.Identification of resistance to wet bubble disease and genetic diversity in wild and cultivated strains of Agaricus bisporus.International Journal of Molecular Sciences,17:1568
Gams W,Hoozemans ACM,1970.Cladobotryum-konidienformen von Hypomyces-arten.Persoonia,6:95-110
Gea FJ,Navarro MJ,Carrasco J,González AJ,Suz LM,2012.First report of cobweb on white button mushroom(Agaricus bisporus)in Spain caused by Cladobotryum mycophilum.Plant Disease,96:1067
Gea FJ,Navarro MJ,Suz LM,2011.First Report of Cladobotryum mycophilum causing cobweb on cultivated king oyster mushroom in Spain.Plant Disease,95:1030
Gea FJ,Navarro MJ,Suz LM,2018a.First report of cobweb disease caused by Cladobotryum dendroides on shiitake mushroom(Lentinula edodes)in Spain.Plant Disease,102:1030
Gea FJ,Navarro MJ,Suz LM,2018b.Cobweb disease on oyster culinary-medicinal mushroom(Pleurotus ostreatus)caused by the mycoparasite Cladobotryum mycophilum.Journal of Plant Pathology,doi:10.1007/s42161-018-0174-z
Grogan HM,Gaze RH,2000.Fungicide resistance among Cladobotryum spp.-causal agents of cobweb disease of the edible mushroom Agaricus bisporus.Mycological Research,104:357-364
Huang NL,Lin ZB,Chen GL,2010.Medicinal and edible Fungi.Shanghai Scientific and Technological Literature Publishing House,Shanghai.1401-1404(in Chinese)
Kang HJ,Sigler L,Lee JK,Gibas CFC,Yun SH,Lee YW,2010.Xylogone ganodermophthora sp.nov.an ascomycetous pathogen causing yellow rot on cultivated mushroom Ganoderma lucidum in Korea.Mycologia,102(5):1167-1184
Kim HK,Seok SJ,Kim JP,Moon BJ,Terashita T,1999.Occurrence of disease caused by Cladobotryum varium on Flammulina velutipes in Korea.The Korean Journal of Mycology,27:415-419
Kim MK,Lee YH,Cho KM,Lee JY,2012.First report of cobweb disease caused by Cladobotryum mycophilum on the edible mushroom Pleurotus eryngii in Korea.Plant Disease,96:1374
Kim TS,Lee HW,Song GW,Shin WG,1998.King oyster mushroom(Pleurotus eryngii)white mold disease caused by Cladobotryum varium.KSM Newsletters,11:46
Lan YF,An XR,Wang QW,Tang LN,2017.Biological characteristics of Cladobotryum mycophilum causing cobweb disease on Agaricus bisporus.Edible Fungi of China,36(4):62-65(in Chinese)
Lan YF,Wang QW,Yu CX,Cong QQ,Tang LN,An XR,Kong FH,Li XD,2016.First Report of Cladobotryum mycophilum causing cobweb disease on Agaricus bisporus in China.Plant Disease,100(11):2334
Li XH,Du SP,Wang YG,Wang J,2011.Common physiological disease and control methods of bag-planted Ganoderma lucidum.Edible Fungi,33(5):58-58(in Chinese)
Li Y,2018.The status,opportunities and challenges of edible fungal industry in China:develop with Chinese characteristics,realizing the dream of powerful mushroom industrial country.Journal of Fungal Research,16(3):125-131(in Chinese)
Liu ZH,Li D,Leo SF,Shen HY,Azu OB,Fu YP,2018.Major pathogenic fungi and bacteria in edible fungi.Journal of Fungal Research,16(3):158-163(in Chinese)
Mckay GJ,Egan D,Morris E,Scott C,Brown AE,1999.Genetic and morphological characterization of Cladobotryum species causing cobweb disease of mushrooms.Applied and Environmental Microbiology,65:606-610
Seifert K,Morgan-Jones G,Gams W,Kendrick B,2011.The genera of Hyphomycetes.CBS-KNAW Fungal Biodiversity Centre,Utrecht.1-997
Sun P,2001.Occurrence and control technology of Ganoderma lucidum insect pests and diseases.Plant Doctor,14(5):21-21(in Chinese)
Tan W,Zhou J,Zhang B,Li XL,Miao RY,Huang ZQ,Yan SJ,2018.Investigation on cultivation status of Ganoderma lucidum and analysis on cultivation technology demand.Science and Technology of Sichuan Agriculture,1:11-12(in Chinese)
Wang GZ,Guo MP,Bian YB,2015.First report of Cladobotryum protrusum causing cobweb disease on the edible mushroom Coprinus comatus.Plant Disease,99(2):287-287
Wang GZ,Ma CJ,Zhou SS,P?ldmaa K,Tamm H,Luo Y,Guo MP,Ma XL,Bian YB,Zhou Y,2018.First report of cobweb disease of Auricularia polytricha caused by Cladobotryum cubitense in Xuzhou,China.Plant Disease,102:1452
Wang YP,2009.Investigations on disease and insect pest species of Ganoderma lucidum.Anhui Agricultural Science Bulletin,15(1):136-137(in Chinese)
Xie YL,2013.Investigation of the diseases of Ganoderma lucidum in Anhui.Edible Fungi of China,32(6):45-47(in Chinese)
Zhang QH,Wang W,Li CH,Wen ZQ,2015.Biological characteristics of Hypomyces aurantius parasitic on Hypsizygus marmoreus.Mycosystema,34(3):350-356(in Chinese)
Zuo B,Lu BH,Liu XL,Wang Y,Ma GL,Gao J,2016.First report of Cladobotryum mycophilum causing cobweb on Ganoderma lucidum cultivated in Jilin province,China.Plant Disease,100:1239
蔡为明,方菊莲,范雷法,魏海红,1993.金针菇软腐病病原菌鉴定及生物学特性的观察.浙江农业学报,5(2):99-103
曹妮娜,王育娜,2015.蓝田县灵芝栽培中常见病虫害及防治方法.食用菌,37(6):56-57
戴玉成,曹云,周丽伟,吴声华,2013.中国灵芝学名之管见.菌物学报,32:947-952
方中达,1998.植病研究方法.第3版.北京:中国农业出版社.122-145
黄年来,林志彬,陈国良,2010.中国食药用菌学.上海:上海科学技术文献出版社.1401-1404
兰玉菲,安秀荣,王庆武,唐丽娜,2017.双孢蘑菇蛛网病病菌生物学特性研究.中国食用菌,36(4):62-65
李现合,杜适普,王亚光,王炯,2011.袋栽灵芝常见的生理性病害及防治方法.食用菌,33(5):58-58
李玉,2018.中国食用菌产业发展现状、机遇和挑战--走中国特色菇业发展之路,实现食用菌产业强国之梦.菌物研究,16(3):125-131
刘正慧,李丹,SOSSAH Frederick Leo,沈宏艳,OKORLEYBenjamin Azu,付永平,2018.食用菌主要病原真菌和细菌.菌物研究,16(3):158-163
孙平,2001.灵芝病虫害的发生与防治技术.植物医生,14(5):21-21
谭伟,周洁,张波,李小林,苗人云,黄忠乾,闫世杰,2018.灵芝栽培现状调研及栽培技术需求分析.四川农业科技,1:11-12
汪义平,2009.灵芝病虫害种类调查.安徽农学通报,15(1):136-137
解宜林,2013.安徽灵芝病害调查与防治.中国食用菌,32(6):45-47
张琪辉,王威,李成欢,温志强,2015.斑玉蕈蛛网病的病原菌及其生物学特性.菌物学报,34(3):350-356
Back CG,Lee CY,Seo GS,Jung HY,2012.Characterization of species of Cladobotryum which cause cobweb disease in edible mushrooms grown in Korea.Mycobiology,40:189-194
Cai WM,Fang JL,Fan LF,Wei HH,1993.Identification and biological characters of the fungal pathogens of the soft rot of Flammulina velutipes.Acta Agriculturae Zhejiangensis,5(2):99-103(in Chinese)
Cao NN,Wang YN,2015.Insect pests and diseases in the cultivation of Ganoderma lucidum in Lantian county.Edible Fungi,37(6):56-57(in Chinese)
Cao Y,Wu SH,Dai YC,2012.Species clarification of the prize medicinal Ganoderma mushroom“Lingzhi”.Fungal Diversity,56:49-62
Carrasco J,Navarro MJ,Gea FJ,2017.Cobweb,a serious pathology in mushroom crops:a review.Spanish Journal of Agricultural Research,15(2):e10R01
Chang GB,Chang YL,Geon SS,Hee YJ,2012.First occurrence of cobweb disease on Hypsizigus marmoreus caused by Cladobotryum varium in Korea.Journal of the Faculty of Agriculture Kyushu University,57(2):373-377
Dai YC,Cao Y,Zhou LW,Wu SH,2013.Notes on the nomenclature of the most widely cultivated Ganoderma species in China.Mycosystema,32:947-952(in Chinese)
Fang ZD,1998.Methodology on plant pathology research.3rd ed.China Agricultural Press,Beijing.122-145(in Chinese)
Fu YP,Wang XX,Li D,Liu Y,Song B,Zhang CL,Wang Q,Chen MY,Zhang ZW,Li Y,2016.Identification of resistance to wet bubble disease and genetic diversity in wild and cultivated strains of Agaricus bisporus.International Journal of Molecular Sciences,17:1568
Gams W,Hoozemans ACM,1970.Cladobotryum-konidienformen von Hypomyces-arten.Persoonia,6:95-110
Gea FJ,Navarro MJ,Carrasco J,González AJ,Suz LM,2012.First report of cobweb on white button mushroom(Agaricus bisporus)in Spain caused by Cladobotryum mycophilum.Plant Disease,96:1067
Gea FJ,Navarro MJ,Suz LM,2011.First Report of Cladobotryum mycophilum causing cobweb on cultivated king oyster mushroom in Spain.Plant Disease,95:1030
Gea FJ,Navarro MJ,Suz LM,2018a.First report of cobweb disease caused by Cladobotryum dendroides on shiitake mushroom(Lentinula edodes)in Spain.Plant Disease,102:1030
Gea FJ,Navarro MJ,Suz LM,2018b.Cobweb disease on oyster culinary-medicinal mushroom(Pleurotus ostreatus)caused by the mycoparasite Cladobotryum mycophilum.Journal of Plant Pathology,doi:10.1007/s42161-018-0174-z
Grogan HM,Gaze RH,2000.Fungicide resistance among Cladobotryum spp.-causal agents of cobweb disease of the edible mushroom Agaricus bisporus.Mycological Research,104:357-364
Huang NL,Lin ZB,Chen GL,2010.Medicinal and edible Fungi.Shanghai Scientific and Technological Literature Publishing House,Shanghai.1401-1404(in Chinese)
Kang HJ,Sigler L,Lee JK,Gibas CFC,Yun SH,Lee YW,2010.Xylogone ganodermophthora sp.nov.an ascomycetous pathogen causing yellow rot on cultivated mushroom Ganoderma lucidum in Korea.Mycologia,102(5):1167-1184
Kim HK,Seok SJ,Kim JP,Moon BJ,Terashita T,1999.Occurrence of disease caused by Cladobotryum varium on Flammulina velutipes in Korea.The Korean Journal of Mycology,27:415-419
Kim MK,Lee YH,Cho KM,Lee JY,2012.First report of cobweb disease caused by Cladobotryum mycophilum on the edible mushroom Pleurotus eryngii in Korea.Plant Disease,96:1374
Kim TS,Lee HW,Song GW,Shin WG,1998.King oyster mushroom(Pleurotus eryngii)white mold disease caused by Cladobotryum varium.KSM Newsletters,11:46
Lan YF,An XR,Wang QW,Tang LN,2017.Biological characteristics of Cladobotryum mycophilum causing cobweb disease on Agaricus bisporus.Edible Fungi of China,36(4):62-65(in Chinese)
Lan YF,Wang QW,Yu CX,Cong QQ,Tang LN,An XR,Kong FH,Li XD,2016.First Report of Cladobotryum mycophilum causing cobweb disease on Agaricus bisporus in China.Plant Disease,100(11):2334
Li XH,Du SP,Wang YG,Wang J,2011.Common physiological disease and control methods of bag-planted Ganoderma lucidum.Edible Fungi,33(5):58-58(in Chinese)
Li Y,2018.The status,opportunities and challenges of edible fungal industry in China:develop with Chinese characteristics,realizing the dream of powerful mushroom industrial country.Journal of Fungal Research,16(3):125-131(in Chinese)
Liu ZH,Li D,Leo SF,Shen HY,Azu OB,Fu YP,2018.Major pathogenic fungi and bacteria in edible fungi.Journal of Fungal Research,16(3):158-163(in Chinese)
Mckay GJ,Egan D,Morris E,Scott C,Brown AE,1999.Genetic and morphological characterization of Cladobotryum species causing cobweb disease of mushrooms.Applied and Environmental Microbiology,65:606-610
Seifert K,Morgan-Jones G,Gams W,Kendrick B,2011.The genera of Hyphomycetes.CBS-KNAW Fungal Biodiversity Centre,Utrecht.1-997
Sun P,2001.Occurrence and control technology of Ganoderma lucidum insect pests and diseases.Plant Doctor,14(5):21-21(in Chinese)
Tan W,Zhou J,Zhang B,Li XL,Miao RY,Huang ZQ,Yan SJ,2018.Investigation on cultivation status of Ganoderma lucidum and analysis on cultivation technology demand.Science and Technology of Sichuan Agriculture,1:11-12(in Chinese)
Wang GZ,Guo MP,Bian YB,2015.First report of Cladobotryum protrusum causing cobweb disease on the edible mushroom Coprinus comatus.Plant Disease,99(2):287-287
Wang GZ,Ma CJ,Zhou SS,P?ldmaa K,Tamm H,Luo Y,Guo MP,Ma XL,Bian YB,Zhou Y,2018.First report of cobweb disease of Auricularia polytricha caused by Cladobotryum cubitense in Xuzhou,China.Plant Disease,102:1452
Wang YP,2009.Investigations on disease and insect pest species of Ganoderma lucidum.Anhui Agricultural Science Bulletin,15(1):136-137(in Chinese)
Xie YL,2013.Investigation of the diseases of Ganoderma lucidum in Anhui.Edible Fungi of China,32(6):45-47(in Chinese)
Zhang QH,Wang W,Li CH,Wen ZQ,2015.Biological characteristics of Hypomyces aurantius parasitic on Hypsizygus marmoreus.Mycosystema,34(3):350-356(in Chinese)
Zuo B,Lu BH,Liu XL,Wang Y,Ma GL,Gao J,2016.First report of Cladobotryum mycophilum causing cobweb on Ganoderma lucidum cultivated in Jilin province,China.Plant Disease,100:1239
蔡为明,方菊莲,范雷法,魏海红,1993.金针菇软腐病病原菌鉴定及生物学特性的观察.浙江农业学报,5(2):99-103
曹妮娜,王育娜,2015.蓝田县灵芝栽培中常见病虫害及防治方法.食用菌,37(6):56-57
戴玉成,曹云,周丽伟,吴声华,2013.中国灵芝学名之管见.菌物学报,32:947-952
方中达,1998.植病研究方法.第3版.北京:中国农业出版社.122-145
黄年来,林志彬,陈国良,2010.中国食药用菌学.上海:上海科学技术文献出版社.1401-1404
兰玉菲,安秀荣,王庆武,唐丽娜,2017.双孢蘑菇蛛网病病菌生物学特性研究.中国食用菌,36(4):62-65
李现合,杜适普,王亚光,王炯,2011.袋栽灵芝常见的生理性病害及防治方法.食用菌,33(5):58-58
李玉,2018.中国食用菌产业发展现状、机遇和挑战--走中国特色菇业发展之路,实现食用菌产业强国之梦.菌物研究,16(3):125-131
刘正慧,李丹,SOSSAH Frederick Leo,沈宏艳,OKORLEYBenjamin Azu,付永平,2018.食用菌主要病原真菌和细菌.菌物研究,16(3):158-163
孙平,2001.灵芝病虫害的发生与防治技术.植物医生,14(5):21-21
谭伟,周洁,张波,李小林,苗人云,黄忠乾,闫世杰,2018.灵芝栽培现状调研及栽培技术需求分析.四川农业科技,1:11-12
汪义平,2009.灵芝病虫害种类调查.安徽农学通报,15(1):136-137
解宜林,2013.安徽灵芝病害调查与防治.中国食用菌,32(6):45-47
张琪辉,王威,李成欢,温志强,2015.斑玉蕈蛛网病的病原菌及其生物学特性.菌物学报,34(3):350-356