900 MHz手机辐射对人视网膜色素上皮细胞增殖活性及转化生长因子β2表达的影响
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摘要
目的:研究900 MHz手机辐射对人视网膜色素上皮细胞(RPE)增殖活性及转化生长因子β2(TGF-β2)表达的影响。方法:采用体外培养的RPE细胞,给予900 MHz手机电磁辐射者处理作为辐射组,未给予辐射者作为对照组。通过CCK8法检测RPE细胞的增殖活性,Real-Time PCR法检测RPE细胞TGF-β2 m RNA的表达,ELISA法检测RPE细胞培养上清液中TGF-β2的蛋白含量。结果:与对照组相比,辐射组RPE细胞的增殖活性降低,TGF-β2 m RNA表达增加,RPE细胞培养上清中TGF-β2含量上调,且差异具有统计学意义(P<0.05)。结论:900 MHz的手机辐射可能能通过调节TGF-β2的表达和释放导致RPE细胞增殖活性降低相关的眼部疾病。
Objective: To investigate the effect of 900 MHz mobile phone radiation on the proliferative activity and expression of transforming growth factor-β2(TGF-β2) in human retinal pigment epithelium(RPE). Methods: RPE cells were cultured in vitro and divided into two groups. The radiation group was given exposure with a 900 MHz mobile phone in call and the control group was not given any exposure. The proliferation of RPE cells was detected by the cell-counting kit-8(CCK8) assay. The expression of TGF-β2 m RNA and protein secretion of RPE cells were measured by Real-Time quantitative polymerase chain reaction(Real-Time PCR) and Enzymelinked immunosorbent assay(ELISA) respectively. Results: The viability of RPE cells in radiation group was lower compared to that of the control group. The level of TGF-β2 m RNA and secretion were up-regulated under the exposure in contrast with the control group(P<0.05). Conclusions: 900 MHz mobile phone radiation may have an underlying association with some eye diseases related with decreased proliferative activity of RPE cells by regulating the expression and release of TGF-β2.
Objective: To investigate the effect of 900 MHz mobile phone radiation on the proliferative activity and expression of transforming growth factor-β2(TGF-β2) in human retinal pigment epithelium(RPE). Methods: RPE cells were cultured in vitro and divided into two groups. The radiation group was given exposure with a 900 MHz mobile phone in call and the control group was not given any exposure. The proliferation of RPE cells was detected by the cell-counting kit-8(CCK8) assay. The expression of TGF-β2 m RNA and protein secretion of RPE cells were measured by Real-Time quantitative polymerase chain reaction(Real-Time PCR) and Enzymelinked immunosorbent assay(ELISA) respectively. Results: The viability of RPE cells in radiation group was lower compared to that of the control group. The level of TGF-β2 m RNA and secretion were up-regulated under the exposure in contrast with the control group(P<0.05). Conclusions: 900 MHz mobile phone radiation may have an underlying association with some eye diseases related with decreased proliferative activity of RPE cells by regulating the expression and release of TGF-β2.
引文
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[23]刘亚军,闫峰,叶巍,等.转化生长因子-β2(TGF-β2)诱导人视网膜色素上皮层细胞上皮-间质转分化中mi RNA-29b的表达及意义[J].眼科新进展,2016,36(11):1001-1005
[24]李冬平,原莉莉,张东昌.转化生长因子β2对人视网膜色素上皮细胞间质转化的影响[J].山西医药杂志,2017,46(17):2035-2037
[25]王玲,杨丽萍,刘梦迎,等.大鼠球结膜滤过泡模型中转化生长因子β1及转化生长因子β2动态表达的实验研究[J].中国眼耳鼻喉科杂志,2016,16(5):313-318
[26]Jia Y,Hu DN.Human aqueous humor levels of TGF-beta2:relationship with axial length[J].Biomed Res Int,2014:258591
[27]Mao JF,Liu SZ.Mechanism of the DL-alpha-aminoadipic acid inhibitory effect on form-deprived myopia in guinea pig[J].Int J Ophthalmol,2013,6(1):19-22
[2]Pandey N,Giri S,Das S,et al.Radiofrequency radiation(900 MHz)-induced DNA damage and cell cycle arrest in testicular germ cells in swiss albino mice[J].Toxicol Ind Health,2017,33(4):373-384
[3]Coureau G,Bouvier G,Lebailly P,et al.Mobile phone use and brain tumours in the CERENAT case-control study[J].Occup Environ Med,2014,71(7):514-522
[4]El-Gohary OA,Said MA.Effect of electromagnetic waves from mobile phone on immune status of male rats:possible protective role of vitamin D[J].Can J Physiol Pharmacol,2017,95(2):151-156
[5]Ahmadi S,Sadat Alavi S,Jadidi M,et al.Exposure to GSM 900-MHz mobile radiation impaired inhibitory avoidance memory consolidation in rat:involvements of opioidergic and nitrergic systems[J].Brain Res,2018[Epub ahead of print]
[6]史凯,张文芳,刘瑜,等.手机微波辐射对体外培养大鼠晶状体Bax表达的影响[J].国际眼科杂志,2013,13(2):265-266
[7]孙一岚,刘瑜.手机微波辐射对体外培养大鼠晶状体HSP70表达的影响[J].国际眼科杂志,2011,11(4):591-593
[8]孙丽霞,姚克,姜槐,等.手机微波辐照对人晶状体上皮细胞DNA的损伤作用及其对细胞增殖活性的影响[J].中华眼科杂志,2006,42(12):1084-1088
[9]周欣荣,原慧萍,王雅,等.不同强度微波辐射对SD大鼠视网膜神经节细胞凋亡的影响[J].眼科新进展,2007,27(6):429-431
[10]Border WA,Ruoslahti E.Transforming growth factor-beta in disease:the dark side of tissue repair[J].J Clin Invest,1992,90(1):1-7
[11]王洁,朱煌,杜尔罡.极低频电磁场对体外培养人视网膜色素上皮细胞的影响[J].中华眼视光学与视觉科学杂志,2015,17(7):424-428
[12]Zhou Z,Shan J,Zu J,et al.Social behavioral testing and brain magnetic resonance imaging in chicks exposed to mobile phone radiation during development[J].BMC Neurosci,2016,17(1):36
[13]Strauss O.The retinal pigment epithelium in visual function[J].Physiol Rev,2005,85(3):845-881
[14]Dornonville de la Cour M.Ion transport in the retinal pigment epithelium.A study with double barrelled ion-selective microelectrodes[J].Acta Ophthalmol Suppl,1993,(209):1-32
[15]Miyamoto Y,Del Monte MA.Na(+)-dependent glutamate transporter in human retinal pigment epithelial cells[J].Invest Ophthalmol Vis Sci,1994,35(10):3589-3598
[16]Seko Y,Tanaka Y,Tokoro T.Scleral cell growth is influenced by retinal pigment epithelium in vitro[J].Graefes Arch Clin Exp Ophthalmol,1994,232(9):545-552
[17]王槐睿.探究电磁辐射对环境的污染及防护[J].环境与发展,2018,07:30+32
[18]Redlarski G,Lewczuk B,Zak A,et al.The influence of electromagnetic pollution on living organism:historical trends and forecasting changes[J].Biomed Res Int,2015:234098
[19]Pandey N,Giri S.Melatonin attenuates radiofrequency radiation(900MHz)-induced oxidative stress,DNA damage and cell cycle arrest in germ cells of male Swiss albino mice[J].Toxicology and industrial health,2018,34(5):315-327
[20]Nikzad S,Mahmoudi G,Amini P,et al.Effects of radiofrequency radiation in the presence of gold nanoparticles for the treatment of renal cell carcinoma[J].Journal of renal injury prevention,2017,6(2):103-108
[21]Chen C,Ma Q,Liu C,et al.Exposure to 1800 MHz radiofrequency radiation impairs neurite outgrowth of embryonic neural stem cells[J].Science reports,2014,4:5103
[22]Rocha Junior AM,Vieira BJ,de Andrade LC et al.Low-level laser therapy increases transforming growth factor-beta2 expression and induces apoptosis of epithelial cells during the tissue repair process[J].Photomedicine and laser surgery,2009,27(2):303-307
[23]刘亚军,闫峰,叶巍,等.转化生长因子-β2(TGF-β2)诱导人视网膜色素上皮层细胞上皮-间质转分化中mi RNA-29b的表达及意义[J].眼科新进展,2016,36(11):1001-1005
[24]李冬平,原莉莉,张东昌.转化生长因子β2对人视网膜色素上皮细胞间质转化的影响[J].山西医药杂志,2017,46(17):2035-2037
[25]王玲,杨丽萍,刘梦迎,等.大鼠球结膜滤过泡模型中转化生长因子β1及转化生长因子β2动态表达的实验研究[J].中国眼耳鼻喉科杂志,2016,16(5):313-318
[26]Jia Y,Hu DN.Human aqueous humor levels of TGF-beta2:relationship with axial length[J].Biomed Res Int,2014:258591
[27]Mao JF,Liu SZ.Mechanism of the DL-alpha-aminoadipic acid inhibitory effect on form-deprived myopia in guinea pig[J].Int J Ophthalmol,2013,6(1):19-22
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