电解强化人工湿地处理城市污水处理厂尾水中微生物群落分析
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摘要
一个新型的电解人工湿地能够成功地对污水处理厂尾水中的氮、磷进行深度脱除,但其中的微生物机理和人工湿地对受水河道微生物生态的影响还远未清楚。采用高通量测序和网络分析对电解强化人工湿地中的功能微生物群落进行了研究,结果显示,电解强化人工湿地中有大量的自养型反硝化功能菌,从而保障了在低碳氮比下污水处理厂尾水中氮的脱除。经网络分析还发现,经过电解强化人工湿地后,污水处理厂尾水中的细菌群落与受水河道有显著差异,人工湿地可在污水处理厂与自然水体间起缓冲作用,更有利于减少污水处理厂尾水对自然水体的生态扰动。
A new electrolysis intensified constructed wetland(E-CW) had been constructed for the deep treatment of waste water treatment plants(WWTP) effluent; however, the bacterial communities and activities in this kind of CWs were far from clear. Using high-throughput sequencing and network analysis, the bacterial community associated with nutrient removal was analyzed. The results showed that E-CW harbored large amount of denitrifiers which ensured the removal of nitrogen in WWTP effluent. Furthermore, the difference of dominant OTUs and separated module as shown by network analysis demonstrated minor influence of WWTP bacterial on receiving river.
A new electrolysis intensified constructed wetland(E-CW) had been constructed for the deep treatment of waste water treatment plants(WWTP) effluent; however, the bacterial communities and activities in this kind of CWs were far from clear. Using high-throughput sequencing and network analysis, the bacterial community associated with nutrient removal was analyzed. The results showed that E-CW harbored large amount of denitrifiers which ensured the removal of nitrogen in WWTP effluent. Furthermore, the difference of dominant OTUs and separated module as shown by network analysis demonstrated minor influence of WWTP bacterial on receiving river.
引文
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[18]Ju F,Zhang T.16S rRNA gene high-throughput sequencing data mining of microbial diversity and interactions[J].Applied microbiology and biotechnology,2015,99(10):4119-4129.
[2]甘沁宇,吴贵凉.城市湿地公园中的景观艺术:以成都活水公园为例[J].参花,2013(9):68-68.
[3]Hao R,et al.Denitrification of simulated municipal wastewater treatment plant effluent using a three-dimensional biofilm-electrode reactor:Operating performance and bacterial community[J].Bioresource Technology,2013,143:178-186.
[4]Metcalf&Eddy,Inc.Wastewater Engineering Treatment and Reuse[M].New York:McGraw Hill,2003.
[5]高燕.电解强化人工湿地脱氮除磷过程与机理研究[D].南京:南京大学,2017.
[6]Elsayed O,et al.Bacterial communities in batch and continuous-flow wetlands treating the herbicide S-metolachlor[J].Science of The Total Environment,2014,499:327-335.
[7]MoratóJ,et al.Key design factors affecting microbial community composition and pathogenic organism removal in horizontal subsurface flow constructed wetlands[J].Science of the Total Environment,2014,481:81-89.
[8]Zhong F,et al.Bacterial community analysis by PCR-DGGE and 454-pyrosequencing of horizontal subsurface flow constructed wetlands with front aeration[J].Applied microbiology and biotechnology,2015,99(3):1499-1512.
[9]B o u a l i M,e t a l.B a c t e r i a l s t r u c t u r e a n d spatiotemporal distribution in a horizontal subsurface flow constructed wetland[J].Applied microbiology and biotechnology,2014,98(7):3191-3203.
[10]Quast C,et al.The SILVA ribosomal RNA gene database project:improved data processing and web-based tools[J].Nucleic Acids Research,2013.41(Database issue):590-596.
[11]Erguder T H,et al.Environmental factors shaping the ecological niches of ammonia-oxidizing archaea[J].FEMS microbiology reviews,2009,33(5):855-869.
[12]Novo A,et al.Antibiotic resistance,antimicrobial residues and bacterial community composition in urban wastewater[J].Water research,2013,47(5):1875-1887.
[13]Vandenberg O,et al.Arcobacter species in humans[J].Emerging infectious diseases,2004,10(10):1863-1867.
[14]Brazelton W J,et al.Bacterial Communities Associated with Subsurface Geochemical Processes in Continental Serpentinite Springs[J].Applied and Environmental Microbiology,2013,79(13):3906-3916.
[15]Gücker B,Brauns M,Pusch M T.Effects of wastewater treatment plant discharge on ecosystem structure and function of lowland streams[J].Journal of the North American Benthological Society,2006,25(2):313-329.
[16]Wakelin S A,Colloff M J,Kookana R S.Effect of wastewater treatment plant effluent on microbial function and community structure in the sediment of a freshwater stream with variable seasonal flow[J].Applied and environmental microbiology,2008,74(9):2659-2668.
[17]Gómez J M,VerdúM,Perfectti F.Ecological interactions are evolutionarily conserved across the entire tree of life[J].Nature,2010,465(7300):918-921.
[18]Ju F,Zhang T.16S rRNA gene high-throughput sequencing data mining of microbial diversity and interactions[J].Applied microbiology and biotechnology,2015,99(10):4119-4129.
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