基于网络药理学的补肾益心片治疗高血压分子机制研究
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摘要
目的采用网络药理学方法分析补肾益心片治疗高血压的作用机制,为其临床与应用提供参考。方法检索中药系统药理学数据库分析平台(TCMSP)获取补肾益心片活性成分,运用DRAR-CPI服务器、GeneCards和OMIM等数据库筛选活性成分治疗高血压的作用靶点。采用Cytoscape3.6.0软件构建补肾益心片活性成分-高血压靶点网络。结合String数据库和Cytoscape的NetworkAnalyzer分析蛋白相互作用关系。采用Systems Dock Web Site进行活性成分与靶点分子对接。并进行GO分析、KEGG通路富集分析。结果筛选出补肾益心片活性成分30个,作用于62个靶点,主要通过调节肾素-血管紧张素系统、Toll样受体信号通路、PI3K-AKT信号通路和Jak-STAT信号通路等发挥治疗高血压的作用。结论本研究初步揭示了补肾益心片治疗高血压的多成分、多靶点作用机制,可为后续研究提供参考。
Objective To analyze the mechanism of Bushen Yixin Tablets(BYT) for hypertension based on network pharmacology; To provide references for clinic and application. Methods Active components in BYT were obtained by retrieving TCM systems pharmacology database and analysis platform(TCMSP). The active targets of BYT were screened by using databases such as DRAR-CPI, GeneCards and OMIM. Cytoscape3.6.0 was used to form the active components of BYT-hypertension targets network. String database and Cytoscape NetworkAnalyzer were used to analyze protein interactions. The System Dock Web Site was used to interface the active components with the target molecule. GO analysis and KEGG pathway enrichment analysis were conducted. Results Totally 30 active components of BYT and 62 target were obtained. BYT played the role of treating hypertension through regulating renin-angiotensin system. Toll-like receptor signaling pathway, PI3K-AKT signaling pathway and Jak-STAT signaling pathway. Conclusion This study preliminarily discloses the multi-component and multi-target mechanism of BYT for hypertension, which can provide references for further research.
Objective To analyze the mechanism of Bushen Yixin Tablets(BYT) for hypertension based on network pharmacology; To provide references for clinic and application. Methods Active components in BYT were obtained by retrieving TCM systems pharmacology database and analysis platform(TCMSP). The active targets of BYT were screened by using databases such as DRAR-CPI, GeneCards and OMIM. Cytoscape3.6.0 was used to form the active components of BYT-hypertension targets network. String database and Cytoscape NetworkAnalyzer were used to analyze protein interactions. The System Dock Web Site was used to interface the active components with the target molecule. GO analysis and KEGG pathway enrichment analysis were conducted. Results Totally 30 active components of BYT and 62 target were obtained. BYT played the role of treating hypertension through regulating renin-angiotensin system. Toll-like receptor signaling pathway, PI3K-AKT signaling pathway and Jak-STAT signaling pathway. Conclusion This study preliminarily discloses the multi-component and multi-target mechanism of BYT for hypertension, which can provide references for further research.
引文
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[2]黄火剑.补肾益心片对原发性高血压患者的治疗作用及其机制研究[D].广州:广州中医药大学,2011.
[3]吴伟,刘煜德,陈宏珪,等.补肾益心片对肾血管性高血压大鼠的降压作用及保护靶器官的研究[J].广州中医药大学学报,2001,18(1):63-66.
[4]刘煜德,吴伟,陈宏珪,等.补肾益心片对肾性高血压大鼠血浆过氧化脂质及性激素的影响[J].中药新药与临床药理,2001,12(6):426-427,452.
[5]LUO H,CHEN J,SHI L,et al.DRAR-CPI:a server for identifying drug repositioning potential and adverse drug reactions via the chemical-protein interactome[J].Nucleic Acids Res,2011,39:492-498.
[6]VON MERING C,JENSEN L J,SNEL B,et al.STRING:known and predicted protein-protein associations,integrated and transferred across organisms[J].Nucleic Acids Res,2005,33:433-437.
[7]HSIN K Y,MATSUOKA Y,ASAI Y,et al.Systems dock:a web server for network pharmacology-based prediction and analysis[J].Nucleic Acids Res,2016,44:507-513.
[8]DENNIS G J R,SHERMAN B T,HOSACK D A,et al.DAVID:Database for annotation,visualization,and integrated discovery[J].Genome Biol,2003,4:3.
[9]孙艳香.原发性高血压患者血清中抗AT1受体自身抗体的生物学效应及其产生机制研究[D].武汉:华中科技大学,2009.