小麦低聚肽对小鼠解酒功能的评价
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摘要
目的评价小麦低聚肽对醉酒小鼠的解酒活性,探讨作用机理。方法清洁级雄性KM种小鼠随机分为对照组和低、中、高剂量组(500、1 000、2 000 mg/kg·bw)。受试物按10 ml/kg·bw给予,30 min后,各组分别灌胃56%乙醇。灌胃结束后立即测定行为学指标,30 min后在各时间段分别测定血液中乙醇和乙醛含量,检测肝组织中ADH、ALDH、SOD、CYP450、CAT、XOD活性。结果与对照组相比,小麦低聚肽能降低小鼠全血中乙醇和乙醛含量,增强肝组织中ADH、ALDH含量,提高CAT、SOD活性。结论小麦低聚肽对醉酒小鼠具有解酒功能。
Objective To investigate effect of wheat oligo-peptides(WOP)on antialcoholism function in mice,and to explore the antialcoholic mechanism.Methods Male KM mice were randomly divided into 4 groups,i.e.the model control group,low-dose group,middle-dose group and high-dose group.Dose design was 500,1 000,2 000 mg/kg·bw for WPO.All animals in the tested groups were gavaged with tested materials in 10 ml/kg·bw.After 30 minutes,all mice have gavaged the optimal dose of 56% ethanol.The behavioural indicators and biochemical markers of mice were determined to evaluate the anti-alcoholic activity of WOP.Results Compared with the model group,WOP could reduce the concentrations of ethanol,and acetaldehyde in blood,enhance the activity of ADH,ALDH,CAT,SOD in liver tissue.Conclusion Based on the results of this study,the WOP can enhance the anti alcoholic ability of mice.
Objective To investigate effect of wheat oligo-peptides(WOP)on antialcoholism function in mice,and to explore the antialcoholic mechanism.Methods Male KM mice were randomly divided into 4 groups,i.e.the model control group,low-dose group,middle-dose group and high-dose group.Dose design was 500,1 000,2 000 mg/kg·bw for WPO.All animals in the tested groups were gavaged with tested materials in 10 ml/kg·bw.After 30 minutes,all mice have gavaged the optimal dose of 56% ethanol.The behavioural indicators and biochemical markers of mice were determined to evaluate the anti-alcoholic activity of WOP.Results Compared with the model group,WOP could reduce the concentrations of ethanol,and acetaldehyde in blood,enhance the activity of ADH,ALDH,CAT,SOD in liver tissue.Conclusion Based on the results of this study,the WOP can enhance the anti alcoholic ability of mice.
引文
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[10] 侯艳宁,刘建芳,吴宏海,等.长期给酒精后大鼠不同脑区氨基酸类递质变化及吡拉西坦的作用[J].中国药物依赖性杂志,2001,10(4):261-263,277.
[11] 陈英,朱科学,彭伟,等.小麦胚活性肽对D-半乳糖衰老模型小鼠抗氧化作用研究[J].食品科学,2010,31(19):325-328.
[12] Babini E,Tagliazucchi D,Martini S,et al.LC-ESI-QTOF-MS identification of novel antioxidant peptides obtained by enzymatic and microbial hydrolysis of vegetable proteins[J].Food Chemistry,2017,228:186-196.
[13] 王嘉榕,滕达,田子罡,等.功能性抗氧化肽制备与机制研究进展[J].天然产物研究与开发,2008,20(2):371-375.
[14] 张莉莉,严群芳,王恬.大豆生物活性肽的分离及其抗氧化活性研究[J].食品科学,2007,28(5):208-211.
[2] Suetsuna K,Chen JR.Isolation and characterization of peptides with antioxidant activity derived from wheat gluten[J].Food Science and Technology Research,2007,8(3):227-230.
[3] 代卉,施用晖,韩芳,等.小麦肽免疫活性及抗氧化作用的研究[J].天然产物研究与开发,2009,21(3):473-476.
[4] 潘兴昌,曾瑜,刘文颖,等.玉米低聚肽和姜黄素对小鼠的醒酒功能研究[J].食品与发酵工业,2016,42(11):25-34.
[5] 张自然.醒酒功能性食品及其醒酒机理研究进展[J].钦州学院学报,2012,27(3):66-69.
[6] 钟晓凌,王然.醒酒机理的研究进展[J].理工科研,2009(8):271-272.
[7] Haseba T,Tomita Y,Kurosu M,et al.Dose and time changes in liver alcohol dehydrogenase(ADH)activity during acute alcohol intoxication involve not only class I but also class III ADH and govern elimination rat of blood ethanol[J].Legal Medicine,2003,5:202-211.
[8] Haseba T,Duester G,Shimizu A,et al.In vivo contribution of Class III alcohol dehydrogenase(ADH3)to alcohol metabolism through activation by cytoplasmic solution hydrophobicity[J].Biochimica et Biophysica Acta-Molecular Basis of Disease,2006,1762(3):276-283.
[9] Yamaguchi M,Nishikiori F,Ito M,et al.The effects of corn peptide ingestion on facilitating alcohol metabolism in healthy men[J].Bioscience Biotechnology and Biochemistry,1997,61(9):1474-1481.
[10] 侯艳宁,刘建芳,吴宏海,等.长期给酒精后大鼠不同脑区氨基酸类递质变化及吡拉西坦的作用[J].中国药物依赖性杂志,2001,10(4):261-263,277.
[11] 陈英,朱科学,彭伟,等.小麦胚活性肽对D-半乳糖衰老模型小鼠抗氧化作用研究[J].食品科学,2010,31(19):325-328.
[12] Babini E,Tagliazucchi D,Martini S,et al.LC-ESI-QTOF-MS identification of novel antioxidant peptides obtained by enzymatic and microbial hydrolysis of vegetable proteins[J].Food Chemistry,2017,228:186-196.
[13] 王嘉榕,滕达,田子罡,等.功能性抗氧化肽制备与机制研究进展[J].天然产物研究与开发,2008,20(2):371-375.
[14] 张莉莉,严群芳,王恬.大豆生物活性肽的分离及其抗氧化活性研究[J].食品科学,2007,28(5):208-211.