非洲猪瘟诊断技术研究进展
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摘要
非洲猪瘟是由非洲猪瘟病毒引起的家猪和野猪的传染病,能引起所有品种和年龄猪的一系列综合征。论文从非洲猪瘟临床症状与病理变化、样品采集和实验室诊断等方面对非洲猪瘟诊断技术进行简述,重点介绍了血细胞吸附试验、荧光抗体试验、酶联免疫吸附试验、聚合酶链反应、荧光定量PCR、环介导等温扩增等非洲猪瘟诊断技术的最新研究进展,以期为非洲猪瘟综合防控提供借鉴和参考。
African swine fever is an infectious disease of swine and wild boars caused by African swine fever virus,which can cause a series of symptoms in all different breeds and ages of swine.The diagnostic techniques of ASF were briefly described from the clinical symptoms and pathological lesions,sample collections and laboratory diagnosis of ASF;The latest research progress in diagnostic technology about HAD,FAT,ELISA,PCR,fluorescent quantitative PCR and LAMP was emphasized in order to provide references for comprehensive prevention and control of African swine fever.
African swine fever is an infectious disease of swine and wild boars caused by African swine fever virus,which can cause a series of symptoms in all different breeds and ages of swine.The diagnostic techniques of ASF were briefly described from the clinical symptoms and pathological lesions,sample collections and laboratory diagnosis of ASF;The latest research progress in diagnostic technology about HAD,FAT,ELISA,PCR,fluorescent quantitative PCR and LAMP was emphasized in order to provide references for comprehensive prevention and control of African swine fever.
引文
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[14] 李维彬,蒋正军,王玉炯,等.非洲猪瘟病毒TaqMan探针实时荧光定量PCR检测方法的建立[J].宁夏大学学报:自然科学版,2007,28(1):56-59.
[15] James H E,Ebert K,McGonigle R,et al.Detection of African swine fever virus by loop-mediated isothermal amplification[J].J Virol Meth,2010(164):68-74.
[16] 杨吉飞,关贵全,刘志杰,等.非洲猪瘟病毒环介导恒温扩增快速检测技术的建立及应用[J].中国动物传染病学报,2011,19(4):7-12.
[2] 田克恭,李明.动物疫病诊断技术-理论与应用[M].北京:中国农业出版社,2014:570-575.
[3] Penrith M L,Guberti V,Depner K,et al.Preparation of African swine fever contingency plans[R].Yerevan:Food and Agriculture Organization of the United Nations,2011:77.
[4] Kolbasov D,Titov I,Tsybanov S,et al.African swine fever,Siberia,Russia,2017[J].Emerg Infect Dis,2018,24(4):796-798.
[5] 王清华,任炜杰,包静月,等.我国首例非洲猪瘟的确诊[J/OL].中国动物检疫,2018,http://kns.cnki.net/kcms/detail/37.1246.S.20180807.0908.002.html.
[6] Oura C A,Edwards L,Batten C A,et al.Virological diagnosis of African swine fever-Comparative study of available tests[J].Virus Res,2013(173):150-158.
[7] 梁云浩,曹琛福,陶虹,等.非洲猪瘟病毒P54蛋白的真核表达及间接ELISA检测方法的建立[J].中国兽医科学,2014,44(4):373-378.
[8] 杨吉飞,关贵全,刘志杰,等.一种用于非洲猪瘟病毒检测的PCR方法[J].畜牧兽医学报,2011,42(8):1201-1206.
[9] 曾少灵,花群义,张彩虹,等.非洲猪瘟病毒PCR检测方法的建立[J].动物医学进展,2009,30(10):6-10
[10] 胡凌,王印,杨泽晓,等.同时检测五种引起猪繁殖障碍的病毒的多重PCR的建立[J].中国兽医科学,2015,45(10):1047-1052.
[11] Zsak L,Borca M V,Risatti G R,et al.Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay[J].J Clin Microbiol,2005,43(1):112-119.
[12] 张泉,朱鸿飞,孙怀昌.非洲猪瘟病毒常规PCR及Real-time PCR检测方法的建立[J].中国预防兽医学报,2017,29(6):459-461.
[13] 曾少灵,花群义,杨俊兴,等.非洲猪瘟病毒实时荧光PCR检测方法的建立[J].中国兽医学报,2010,30(9):1176-1178.
[14] 李维彬,蒋正军,王玉炯,等.非洲猪瘟病毒TaqMan探针实时荧光定量PCR检测方法的建立[J].宁夏大学学报:自然科学版,2007,28(1):56-59.
[15] James H E,Ebert K,McGonigle R,et al.Detection of African swine fever virus by loop-mediated isothermal amplification[J].J Virol Meth,2010(164):68-74.
[16] 杨吉飞,关贵全,刘志杰,等.非洲猪瘟病毒环介导恒温扩增快速检测技术的建立及应用[J].中国动物传染病学报,2011,19(4):7-12.