基于淀粉酶糖化力检测的麦芽药材质量控制
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摘要
目的:建立麦芽药材糖化力检测方法,更有效地对麦芽药材进行质量控制。方法:采用间接碘量法,以糖化力为指标,单因素考察粉碎粒径、提取时间、提取温度、提取次数及溶媒倍数,并采用L9(34)正交试验优化麦芽提取方法。结果:确定最佳的提取方法为1.0 g麦芽粉末(过3号筛)加100 m L水,30℃浸渍1 h。该方法精密度和重复性RSD%分别为1.33%和1.35%,以9批麦芽药材对该方法进一步验证。结论:所得麦芽提取方法操作简便稳定,精密度和重复性均良好,说明该方法稳定可靠,可用于麦芽的质量控制。
Objective: Method of amylase saccharifying power detection was established for improving the quality control of barley malt. Methods: The amylase saccharifying power was detected by indirect iodometry to investigate comminution granularity, material to liquid radio, temperature, time, extraction times as single-factors.Then the extraction process of barley malt was optimized by L9(34) orthogonal test. Results: The optimal extraction process was to extract 1 g barley malt an hour in 100 m L water in 30 ℃ after No. 3 mesh filtration. The precision and repeatability of this method RSD% were 1.33% and 1.35%, respectively. Finally, the method was verified by detecting the amylase saccharifying power of 9 batches of barley malt. Conclusion: The extraction process is convenient and stable, the method is effective and reliable with good precision and repeatability. It is suitable for the improvement of quality control of barley malt.
Objective: Method of amylase saccharifying power detection was established for improving the quality control of barley malt. Methods: The amylase saccharifying power was detected by indirect iodometry to investigate comminution granularity, material to liquid radio, temperature, time, extraction times as single-factors.Then the extraction process of barley malt was optimized by L9(34) orthogonal test. Results: The optimal extraction process was to extract 1 g barley malt an hour in 100 m L water in 30 ℃ after No. 3 mesh filtration. The precision and repeatability of this method RSD% were 1.33% and 1.35%, respectively. Finally, the method was verified by detecting the amylase saccharifying power of 9 batches of barley malt. Conclusion: The extraction process is convenient and stable, the method is effective and reliable with good precision and repeatability. It is suitable for the improvement of quality control of barley malt.
引文
[1]黄兆胜《.中药学》[M].北京:人民卫生出版社,2002:259-260.
[2]陈运中.淀粉酶的性质和分类[J].武汉粮食工业学院学报,1990,9(1):15-22.
[3]国家药典委员会.中国药典[M].一部.北京:中国医药科技出版社,2015:156.
[4]日本药局方《.日本药典》(第十六改正版).[EB/OL].http://jpdb.nihs.go.jp/jp16e
[5]吴琴,陈珣.单因素实验结合响应面法优化朱砂根总皂苷的提取工艺[J].医药导报,2014,29(8):1080-1082.
[6]田亚平,郭鸿飞,肖光焰,等.一种麦芽β-淀粉酶的纯化和特性研究[J].食品工业科技,2003,25(9):22-24.
[7]贾旋旋,李俊松,吴皓,等.滴定法测定制天南星中铝离子含量[J].上海中医药杂志,2009,55(12):70-72.
[2]陈运中.淀粉酶的性质和分类[J].武汉粮食工业学院学报,1990,9(1):15-22.
[3]国家药典委员会.中国药典[M].一部.北京:中国医药科技出版社,2015:156.
[4]日本药局方《.日本药典》(第十六改正版).[EB/OL].http://jpdb.nihs.go.jp/jp16e
[5]吴琴,陈珣.单因素实验结合响应面法优化朱砂根总皂苷的提取工艺[J].医药导报,2014,29(8):1080-1082.
[6]田亚平,郭鸿飞,肖光焰,等.一种麦芽β-淀粉酶的纯化和特性研究[J].食品工业科技,2003,25(9):22-24.
[7]贾旋旋,李俊松,吴皓,等.滴定法测定制天南星中铝离子含量[J].上海中医药杂志,2009,55(12):70-72.