生物传感器用于微囊藻毒素检测的研究进展
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摘要
微囊藻毒素检测生物传感器结合分子生物学技术和传感技术,将微囊藻毒素浓度信号转变为易于检测读取的电化学、光学等信号,具有操作简单、快速等优点。该文主要从电极修饰特点、检出限、检测时间、便捷性、实际应用等方面论述了当前国内外多种生物传感器在微囊藻毒素检测领域的研究进展;其发展趋势是与流动注射等技术相结合,灵敏度不断提高、检测步骤不断简化,操作更加方便,耗时更短,逐步实现多通道自动化、在线实时监测。
Biosensors for detecting microcystins are user-friendly devices, which combine biological components such as antibody, antigen, enzyme with physicochemical detectors and can transform the signal of microcystins' concentration into easily quantified signal such as optical, electrical signal. The biosensors' electrode modification, detection limit, detection time and application for real sample determination were described in this review. The biosensors are becoming more sensitive, simplified, faster, more convenient to use and even automated for real-time on-line detection due to flow injection technology.
Biosensors for detecting microcystins are user-friendly devices, which combine biological components such as antibody, antigen, enzyme with physicochemical detectors and can transform the signal of microcystins' concentration into easily quantified signal such as optical, electrical signal. The biosensors' electrode modification, detection limit, detection time and application for real sample determination were described in this review. The biosensors are becoming more sensitive, simplified, faster, more convenient to use and even automated for real-time on-line detection due to flow injection technology.
引文
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[2]GB5749-2006,生活水饮用卫生标准[S].
[3]WHO.Guidelines for drinking-water quality.Third edition,Geneva:World Health Organization[S].
[4]Fladmark KE,Serres MH,Larsen NL,et al.Sensitive detection of apoptogenic toxins in suspension cultures of ratand salmon hepatocytes[J].Toxicon,1998(36):1101-1114.
[5]徐立红,陈加平,许建明等.用蛋白磷酸酶抑制法测定水体中的微囊藻毒素类物质[J].环境科学学报,1999,19(5):536-539.
[6]Shamsollahi HR,Alimohammadi M,Nabizadeh R,et al.Measurement of microcystin-LR in water samples using improved HPLC method[J].Global Journal of Health Science,2015,7(2):66-70.
[7]Nagata S,Tsutsumi T,Hasegawa A,et al.Enzyme immunoassay for direct determination of microcystins in environmental water[J].Journal of Aoac International,1997,80(2):408-417.
[8]王蕾,李小艳,张惠等.高效液相色谱-质谱法测定蓝藻中的微囊藻毒素[J].食品安全,2007,28(3):197-199.
[9]GB/T20466-2006,水中微囊藻毒素的测定[S].
[10]龙峰,施汉昌,朱安娜等.pH和铜离子对微囊藻毒素-LR荧光免疫检测的影响与消除[J].环境科学,2009,30(7):1974-1977.
[11]张琳,李晶晶,高庚申.微囊藻毒素的酶联免疫法分析影响因素探讨[J].环境污染与防治,2012,34(4):57-62.
[12]Savenije HHG.A one-dimensional model for salinity intrusion in alluvial estuaries[J].Journal of Hydrology,1986,85(1-2):87-109.
[13]Campas M,Marty JL.Highly sensitive amperometric immunosensors for microcystin detection in algae[J].Biosensors and Bioelectronics,2007,22(6):1034-1040.
[14]Zhang J,Lei J,Xu C,et al.Carbon nanohorn sensitized electrochemical immunosensor for rapid detection of microcystinLR[J].Analytical Chemistry,2010,82(3):1117-1122.
[15]Zhao H,Tian J,Quan X.A graphene and multienzyme functionalized carbon nanosphere-based electrochemical immunosensor for microcystin-LR detection[J].Colloids Surf B Biointe rfaces.2013,103(1):38-44.
[16]张晶晶,康天放,刘锦华等.纳米金信号探针/石墨烯修饰免疫传感器检测微囊藻毒素[J].分析测试学报,2017,36(9):1075-1080.
[17]Lebogang L,Jantra J,Hedstr?m M,et al.Electrochemical FlowELISA for Rapid and Sensitive Determination of Microcystin-LR Using Automated Sequential Injection System[J].Sensors,2017,17(7):1639.
[18]Loyprasert S,Thavarungkul P,Asawatreratanakul P,et al.Label-free capacitive immunosensor for microcystin-LR using selfassembled thiourea monolayer incorporated with Ag nanoparticles on gold electrode[J].Biosens Bioelectron,2008,24(1):78-86.
[19]Sun X,Guan L,Shi H,et al.Determination of microcystin-LRwith a glassy carbon impedimetric immunoelectrode modified with an ionic liquid and multiwalled carbon nanotubes[J].Microchimica Acta,2013,180(1-2):75-83.
[20]Fu X,Feng Y,Niu S,et al.Sensitive detection of microcystinLR by using a label-free electrochemical immunosensor based on Au nanoparticles/silicon templateimethylene blue nanocomposite[J].Journal of Nanoscience and Nanotechnology,2013,13(12):8245-8252.
[21]Li R,Xia Q,Li Z,et al.Electrochemical immunosensor for ultrasensitive detection of microcystin-LR based on graphene-gold nanocomposite/functional conducting polymer/gold nanoparticle/ionic liquid composite film with electrodeposition[J].Biosens Bioelectron.2013,44(11):235-240.
[22]Lebogang L,Hedstr?m M,Mattiasson B.Development of a real-time capacitive biosensor for cyclic cyanotoxic peptides based on Adda-specific antibodies[J].Analytica Chimica Acta,2014(826):69-76.
[23]石慧,孙秀兰,王洪新等.微囊藻毒素-LR阻抗型电化学免疫传感器的研究[J].分析测试学报,2009,28(6):633-637.
[24]Sun X,Shi H,Wang H,et al.A Simple,Highly Sensitive,and Label-Free Impedimetric Immunosensor for Detection of MicrocystinLR in Water[J].Analytical Letters,2010,43(4):533-544.
[25]Tong P,Tang S,He Y,et al.Label-free immunosensing of microcystin-LR using a gold electrode modified with gold nanoparticles[J].Microchimica Acta,2011,173(3-4):299-305.
[26]赵晓琴,陈金媛,宋莉莉.纳米TiO2管电化学生物传感器用于测定水体中微囊藻毒素[J].浙江工业大学学报,2012,40(2):148-151.
[27]张金果,康天放,薛瑞等.基于Fe3O4@Au磁性纳米粒子修饰丝网印刷电极的微囊藻毒素免疫传感器研究[J].分析化学,2013,41(9):1353-1358.
[28]杜华丽,符雪文,温永平等.基于石墨烯和金纳米笼修饰的无标记型微囊藻毒素免疫传感器的研制[J].分析化学(FENXI HUAXUE)研究报告,2014,42(5):660-665.
[29]张新爱,申建忠,张帆等.碳纳米管/纳米金复合膜电化学免疫传感器用于微囊藻毒素的检测研究[J].分析化学,2014,42(9):1301-1306.
[30]张晶晶,康天放,鲁理平等.基于四氧化三铁磁性纳米粒子的微囊藻毒素免疫传感器研究[J].分析化学,2016,44(5):760-766.
[31]Chen H,Wang X,Zhan S,et al.A miniature surface plasmon resonance bioanalytical system for field detection of microcystin-LR in surface water[J].Instrumentation Science and Technology,2011,39(5):462-474.
[32]Hu C,Gan N,Chen Y,et al.Detection of microcystins in environmental samples using surface plasmon resonance biosensor[J].Talanta,2009,80(1):407-410.
[33]Herranz S,BockováM,Marazuela MD,et al.An SPRbiosensor for the detection of microcystins in drinking water[J].Anal Bioanal Chem,2010,398(6):2625-2634.
[34]Long F,He M,Zhu AN,et al.Portable optical immunosensor for highly sensitive detection of microcystin-LR in water samples[J].Biosens Bioelectron.2009,24(8),2346-2351.
[35]Herranz S,Marazuela MD,Moreno-Bondi MC.Automated portable array biosensor for multisample microcystin analysis in freshwater samples[J].Biosens Bioelectron.2012,33(1):50-55.
[36]Shi HC,Song BD,Long F,et al.Automated online optical biosensing system for continuous real-time determination of microcystin-LR with high sensitivity and specificity:early warning for cyanotoxin risk in drinking water sources[J].Environmental Science&Technology,2013,47(9):4434-4441.
[37]Liu M,Zhao H,Chen S,et al.Colloidal graphene as a transducer in homogeneous fluorescence-based immunosensor for rapid and sensitive analysis of microcystin-LR[J].Environ Sci Techn ol,2012,46(22),12567-12574.
[38]Shi Y,Wu J,Sun Y,et al.A grapheme oxide based biosensor for microcystins detection by fluorescence resonance energy transfer[J].Biosens Bioelectron.2012,38(1),31-36.
[39]Tian J,Zhao H,Zhao H,et al.Photoelectrochemical immunoassay for microcystin-LR based on a fluorine-doped tin oxide glass electrode modified with a CdS-graphene composite[J].Microchimica Acta,2012,179(1-2):163-170.
[40]Yang M,Lam PK,Huang M,et al.Effects of microcystins on phosphorylase-abinding to phosphatase-2A:kinetic analysis by surface plasmon resonance biosensor[J].Biochimica Biophysica Acta.1999,14;1427(1):62-73.
[41]Campàs M,Szydlowska D,Campas M,et al.Enzyme inhibitionbased biosensor for theelectrochemical detection of microcystins in natural blooms of cyanobacteria[J].Talanta,2007(72):179-186.
[42]Long F,Zhu A,Sheng JW,et al.Matrix Effects on the Microcystin-LR Fluorescent Immunoassay Based on Optical Biosensor[J].Sensors,2009,9(4):3000-3010.
[2]GB5749-2006,生活水饮用卫生标准[S].
[3]WHO.Guidelines for drinking-water quality.Third edition,Geneva:World Health Organization[S].
[4]Fladmark KE,Serres MH,Larsen NL,et al.Sensitive detection of apoptogenic toxins in suspension cultures of ratand salmon hepatocytes[J].Toxicon,1998(36):1101-1114.
[5]徐立红,陈加平,许建明等.用蛋白磷酸酶抑制法测定水体中的微囊藻毒素类物质[J].环境科学学报,1999,19(5):536-539.
[6]Shamsollahi HR,Alimohammadi M,Nabizadeh R,et al.Measurement of microcystin-LR in water samples using improved HPLC method[J].Global Journal of Health Science,2015,7(2):66-70.
[7]Nagata S,Tsutsumi T,Hasegawa A,et al.Enzyme immunoassay for direct determination of microcystins in environmental water[J].Journal of Aoac International,1997,80(2):408-417.
[8]王蕾,李小艳,张惠等.高效液相色谱-质谱法测定蓝藻中的微囊藻毒素[J].食品安全,2007,28(3):197-199.
[9]GB/T20466-2006,水中微囊藻毒素的测定[S].
[10]龙峰,施汉昌,朱安娜等.pH和铜离子对微囊藻毒素-LR荧光免疫检测的影响与消除[J].环境科学,2009,30(7):1974-1977.
[11]张琳,李晶晶,高庚申.微囊藻毒素的酶联免疫法分析影响因素探讨[J].环境污染与防治,2012,34(4):57-62.
[12]Savenije HHG.A one-dimensional model for salinity intrusion in alluvial estuaries[J].Journal of Hydrology,1986,85(1-2):87-109.
[13]Campas M,Marty JL.Highly sensitive amperometric immunosensors for microcystin detection in algae[J].Biosensors and Bioelectronics,2007,22(6):1034-1040.
[14]Zhang J,Lei J,Xu C,et al.Carbon nanohorn sensitized electrochemical immunosensor for rapid detection of microcystinLR[J].Analytical Chemistry,2010,82(3):1117-1122.
[15]Zhao H,Tian J,Quan X.A graphene and multienzyme functionalized carbon nanosphere-based electrochemical immunosensor for microcystin-LR detection[J].Colloids Surf B Biointe rfaces.2013,103(1):38-44.
[16]张晶晶,康天放,刘锦华等.纳米金信号探针/石墨烯修饰免疫传感器检测微囊藻毒素[J].分析测试学报,2017,36(9):1075-1080.
[17]Lebogang L,Jantra J,Hedstr?m M,et al.Electrochemical FlowELISA for Rapid and Sensitive Determination of Microcystin-LR Using Automated Sequential Injection System[J].Sensors,2017,17(7):1639.
[18]Loyprasert S,Thavarungkul P,Asawatreratanakul P,et al.Label-free capacitive immunosensor for microcystin-LR using selfassembled thiourea monolayer incorporated with Ag nanoparticles on gold electrode[J].Biosens Bioelectron,2008,24(1):78-86.
[19]Sun X,Guan L,Shi H,et al.Determination of microcystin-LRwith a glassy carbon impedimetric immunoelectrode modified with an ionic liquid and multiwalled carbon nanotubes[J].Microchimica Acta,2013,180(1-2):75-83.
[20]Fu X,Feng Y,Niu S,et al.Sensitive detection of microcystinLR by using a label-free electrochemical immunosensor based on Au nanoparticles/silicon templateimethylene blue nanocomposite[J].Journal of Nanoscience and Nanotechnology,2013,13(12):8245-8252.
[21]Li R,Xia Q,Li Z,et al.Electrochemical immunosensor for ultrasensitive detection of microcystin-LR based on graphene-gold nanocomposite/functional conducting polymer/gold nanoparticle/ionic liquid composite film with electrodeposition[J].Biosens Bioelectron.2013,44(11):235-240.
[22]Lebogang L,Hedstr?m M,Mattiasson B.Development of a real-time capacitive biosensor for cyclic cyanotoxic peptides based on Adda-specific antibodies[J].Analytica Chimica Acta,2014(826):69-76.
[23]石慧,孙秀兰,王洪新等.微囊藻毒素-LR阻抗型电化学免疫传感器的研究[J].分析测试学报,2009,28(6):633-637.
[24]Sun X,Shi H,Wang H,et al.A Simple,Highly Sensitive,and Label-Free Impedimetric Immunosensor for Detection of MicrocystinLR in Water[J].Analytical Letters,2010,43(4):533-544.
[25]Tong P,Tang S,He Y,et al.Label-free immunosensing of microcystin-LR using a gold electrode modified with gold nanoparticles[J].Microchimica Acta,2011,173(3-4):299-305.
[26]赵晓琴,陈金媛,宋莉莉.纳米TiO2管电化学生物传感器用于测定水体中微囊藻毒素[J].浙江工业大学学报,2012,40(2):148-151.
[27]张金果,康天放,薛瑞等.基于Fe3O4@Au磁性纳米粒子修饰丝网印刷电极的微囊藻毒素免疫传感器研究[J].分析化学,2013,41(9):1353-1358.
[28]杜华丽,符雪文,温永平等.基于石墨烯和金纳米笼修饰的无标记型微囊藻毒素免疫传感器的研制[J].分析化学(FENXI HUAXUE)研究报告,2014,42(5):660-665.
[29]张新爱,申建忠,张帆等.碳纳米管/纳米金复合膜电化学免疫传感器用于微囊藻毒素的检测研究[J].分析化学,2014,42(9):1301-1306.
[30]张晶晶,康天放,鲁理平等.基于四氧化三铁磁性纳米粒子的微囊藻毒素免疫传感器研究[J].分析化学,2016,44(5):760-766.
[31]Chen H,Wang X,Zhan S,et al.A miniature surface plasmon resonance bioanalytical system for field detection of microcystin-LR in surface water[J].Instrumentation Science and Technology,2011,39(5):462-474.
[32]Hu C,Gan N,Chen Y,et al.Detection of microcystins in environmental samples using surface plasmon resonance biosensor[J].Talanta,2009,80(1):407-410.
[33]Herranz S,BockováM,Marazuela MD,et al.An SPRbiosensor for the detection of microcystins in drinking water[J].Anal Bioanal Chem,2010,398(6):2625-2634.
[34]Long F,He M,Zhu AN,et al.Portable optical immunosensor for highly sensitive detection of microcystin-LR in water samples[J].Biosens Bioelectron.2009,24(8),2346-2351.
[35]Herranz S,Marazuela MD,Moreno-Bondi MC.Automated portable array biosensor for multisample microcystin analysis in freshwater samples[J].Biosens Bioelectron.2012,33(1):50-55.
[36]Shi HC,Song BD,Long F,et al.Automated online optical biosensing system for continuous real-time determination of microcystin-LR with high sensitivity and specificity:early warning for cyanotoxin risk in drinking water sources[J].Environmental Science&Technology,2013,47(9):4434-4441.
[37]Liu M,Zhao H,Chen S,et al.Colloidal graphene as a transducer in homogeneous fluorescence-based immunosensor for rapid and sensitive analysis of microcystin-LR[J].Environ Sci Techn ol,2012,46(22),12567-12574.
[38]Shi Y,Wu J,Sun Y,et al.A grapheme oxide based biosensor for microcystins detection by fluorescence resonance energy transfer[J].Biosens Bioelectron.2012,38(1),31-36.
[39]Tian J,Zhao H,Zhao H,et al.Photoelectrochemical immunoassay for microcystin-LR based on a fluorine-doped tin oxide glass electrode modified with a CdS-graphene composite[J].Microchimica Acta,2012,179(1-2):163-170.
[40]Yang M,Lam PK,Huang M,et al.Effects of microcystins on phosphorylase-abinding to phosphatase-2A:kinetic analysis by surface plasmon resonance biosensor[J].Biochimica Biophysica Acta.1999,14;1427(1):62-73.
[41]Campàs M,Szydlowska D,Campas M,et al.Enzyme inhibitionbased biosensor for theelectrochemical detection of microcystins in natural blooms of cyanobacteria[J].Talanta,2007(72):179-186.
[42]Long F,Zhu A,Sheng JW,et al.Matrix Effects on the Microcystin-LR Fluorescent Immunoassay Based on Optical Biosensor[J].Sensors,2009,9(4):3000-3010.
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