丝胶对2型糖尿病大鼠胰腺PI3K mRNA表达的影响
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摘要
目的:探讨丝胶对2型糖尿病大鼠胰腺磷脂酰肌醇-3-激酶(phosphatidylinositol3-kinase,PI3K mRNA表达的影响。方法:雄性SD大鼠随机分为3组,正常对照组、糖尿病模型组、丝胶治疗组。以高脂高糖饲料喂养联合链脲佐菌素(35mg/kg,2次)注射的方法建立2型糖尿病大鼠模型,丝胶治疗组大鼠给予丝胶(2.4g/kg/d)灌胃。采用葡萄糖氧化酶法检测每组大鼠的血糖,Real Time Q-PCR法检测每组大鼠胰腺PI3K mRNA的表达。结果:与正常对照组比较,糖尿病模型组大鼠的血糖明显升高,胰腺PI3K mRNA的表达明显降低(P<0.05);与糖尿病模型组比较,丝胶治疗组大鼠的血糖明显降低,胰腺PI3KmRNA的表达明显升高(P<0.05)。结论:丝胶可能通过提高胰腺PI3K的表达调节胰岛素PI3K-Akt信号通路,从而起到降低血糖的作用。
Objective: To investigate the effects of sericin on phosphatidylinositol-3-kinase(PI3 K) mRNA expression in pancreas of type 2 diabetic rats. Methods: Male SD rats were randomly divided into three groups: normal control group,diabetic model group and sericin treatment group. The rat model of type 2 diabetes mellitus was established by feeding high-fat and high-sugar diet and intraperitoneal injection of streptozotocin(35 mg/kg, twice). Then the rats in the sericin treatment group were given sericin(2.4 g/kg/d) by gavage. Glucose oxidase method was used to detect the blood glucose of rats, and Real Time Q-PCR were used to detect the expression of PI3 K mRNA in pancreas of rats in each group. Results:Compared with the normal control group, the blood glucose of diabetic rats increased significantly and the expression of PI3 K mRNA in pancreas decreased significantly(P<0.05); Compared with diabetic rats, the blood glucose of sericin treated rats decreased significantly, and the expression of PI3 K mRNA in pancreas increased significantly(P<0.05). Conclusions:Sericin may regulate insulin PI3 K-Akt signaling pathway by increasing the expression of PI3 K in pancreas, thus reducing blood glucose.
Objective: To investigate the effects of sericin on phosphatidylinositol-3-kinase(PI3 K) mRNA expression in pancreas of type 2 diabetic rats. Methods: Male SD rats were randomly divided into three groups: normal control group,diabetic model group and sericin treatment group. The rat model of type 2 diabetes mellitus was established by feeding high-fat and high-sugar diet and intraperitoneal injection of streptozotocin(35 mg/kg, twice). Then the rats in the sericin treatment group were given sericin(2.4 g/kg/d) by gavage. Glucose oxidase method was used to detect the blood glucose of rats, and Real Time Q-PCR were used to detect the expression of PI3 K mRNA in pancreas of rats in each group. Results:Compared with the normal control group, the blood glucose of diabetic rats increased significantly and the expression of PI3 K mRNA in pancreas decreased significantly(P<0.05); Compared with diabetic rats, the blood glucose of sericin treated rats decreased significantly, and the expression of PI3 K mRNA in pancreas increased significantly(P<0.05). Conclusions:Sericin may regulate insulin PI3 K-Akt signaling pathway by increasing the expression of PI3 K in pancreas, thus reducing blood glucose.
引文
[1]詹巾卓,李娜,孙汇,等.PI-3K转导通路在Ⅱ型糖尿病发病机制中作用的研究进展[J].北华大学学报(自然科学版),2012,13(2):186-189.
[2]Wang YM,Lin XF,Shi CM,et al.alpha-Lipoic acid protects 3T3-L1 adipocytes from NYGGF4(PID1)overexpression-induced insulin resistance through increasing phosphorylation of IRS-1and Akt[J].J Bioenerg Biomembr,2012,44(3):357-363.
[3]孟艳秋,刘文虎,刘凤鑫,等.抗2型糖尿病药物研究进展[J].现代药物与临床,2013,28(3):461-464.
[4]Chi M,Ye Y,Zhang XD,et al.Insulin induces drug resistance in melanoma through activation of the PI3K/Akt pathway[J].Drug Des Devel Ther,2014,8:255-262.
[5]Zhang Y,Liu X,Han L,et al.Regulation of lipid and glucose homeostasis by mango tree leaf extract is mediated by AMPKand PI3K/AKT signaling pathways[J].Food Chem,2013,141(3):2896-2905.
[6]王帅,金磊,海春旭,等.PI3K/Akt信号通路在胰岛素抵抗中作用的研究进展[J].毒理学杂志,2015,29(4):313-316.
[7]李时珍.本草纲目[M].北京:人民卫生出版社,1985:1045-1052.
[8]付秀美,马宝君,赵立军,等.丝胶对2型糖尿病大鼠血糖的影响[J].承德医学院学报,2008,25(4):351-353.
[2]Wang YM,Lin XF,Shi CM,et al.alpha-Lipoic acid protects 3T3-L1 adipocytes from NYGGF4(PID1)overexpression-induced insulin resistance through increasing phosphorylation of IRS-1and Akt[J].J Bioenerg Biomembr,2012,44(3):357-363.
[3]孟艳秋,刘文虎,刘凤鑫,等.抗2型糖尿病药物研究进展[J].现代药物与临床,2013,28(3):461-464.
[4]Chi M,Ye Y,Zhang XD,et al.Insulin induces drug resistance in melanoma through activation of the PI3K/Akt pathway[J].Drug Des Devel Ther,2014,8:255-262.
[5]Zhang Y,Liu X,Han L,et al.Regulation of lipid and glucose homeostasis by mango tree leaf extract is mediated by AMPKand PI3K/AKT signaling pathways[J].Food Chem,2013,141(3):2896-2905.
[6]王帅,金磊,海春旭,等.PI3K/Akt信号通路在胰岛素抵抗中作用的研究进展[J].毒理学杂志,2015,29(4):313-316.
[7]李时珍.本草纲目[M].北京:人民卫生出版社,1985:1045-1052.
[8]付秀美,马宝君,赵立军,等.丝胶对2型糖尿病大鼠血糖的影响[J].承德医学院学报,2008,25(4):351-353.