黄杆菌裂解产物对巨噬细胞源性泡沫细胞形成的作用及机制研究
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摘要
目的:探讨黄杆菌裂解产物对巨噬细胞源性泡沫细胞形成的作用及可能的机制。方法:将黄杆菌裂解产物与THP-1细胞进行共培养,观察其对THP-1巨噬细胞及泡沫细胞形成的影响,并检测CD14、TLR4、LDLr三个受体mRNA的表达水平。结果:黄杆菌裂解产物促进THP-1单核细胞向巨噬细胞分化,并促进巨噬细胞源性泡沫细胞的形成,上调CD14、TLR4、LDLr三个受体mRNA的表达水平。结论:黄杆菌裂解产物促进单核巨噬细胞源性泡沫细胞的形成,这可能与其上调LDLr表达有关。
Objective: To investigate the effect of Flavobacterium lysate on macrophage-derived foam cell formation and the possible mechanisms. Methods: Flavobacterium lysate and THP-1 cells were co-cultured,observe its effect on THP-1 macrophages and macrophage-derived foam cell formation,also detect the mRNA expression levels of CD14,TLR4,LDLr. Results: Flavobacterium lysates promote THP-1 monocyte differentiated into macrophage, and promote the formation of macrophage-derived foam cells, increased CD14,TLR4,LDLr mRNA expression. Conclusion: Flavobacterium lysate promotes the formation of macrophage-derived foam cells,which may increase its expression LDLr. Flavobacterium lysate may also up-regulate the expression of CD14 and TLR4 of the monocyte-macrophage cells involved in inflammation of atherosclerosis.
Objective: To investigate the effect of Flavobacterium lysate on macrophage-derived foam cell formation and the possible mechanisms. Methods: Flavobacterium lysate and THP-1 cells were co-cultured,observe its effect on THP-1 macrophages and macrophage-derived foam cell formation,also detect the mRNA expression levels of CD14,TLR4,LDLr. Results: Flavobacterium lysates promote THP-1 monocyte differentiated into macrophage, and promote the formation of macrophage-derived foam cells, increased CD14,TLR4,LDLr mRNA expression. Conclusion: Flavobacterium lysate promotes the formation of macrophage-derived foam cells,which may increase its expression LDLr. Flavobacterium lysate may also up-regulate the expression of CD14 and TLR4 of the monocyte-macrophage cells involved in inflammation of atherosclerosis.
引文
[1]杜海燕,郑英明,林阳.动脉粥样硬化代谢标志物群的研究进展.心肺血管病杂志,2015,34:154-156.
[2]Tang WH,Hazen SL.The contributory role of gut microbiota in cardiovascular disease.J Clin Invest,2014,124:4204-4211.
[3]Serino M,Blasco-Baque V,Nicolas S,et al.Far from the Eyes,Close to the Heart:Dysbiosis of Gut Microbiota and Cardiovascular Consequences.Curr Cardiol Rep,2014,16:540.
[4]李坑,朱光发.肠道菌群与代谢综合征.心肺血管病杂志,2014,33:742-744.
[5]Koren O,Spor A,Felin J,et al.Human oral,gut,and plaque microbiota in patients with atherosclerosis.Proc Natl Acad Sci U S A,2011,108(Suppl 1):4592-4598.
[6]Sekine K,Ohta J,Onishi M,et al.Analysis of antitumor properties of effector cells stimulated with a cell wall preparation(WPG)of Bifidobacterium infantis.Biol Pharm Bull,1995,18:148-153.
[7]许建华,段光杰,朱江,等.分化诱导剂PMA对THP-1源性巨噬细胞膜表面受体MD-2表达的影响.免疫学杂志,2013,29:121-125.
[8]Cao F,Castrillo A,Tontonoz P,et al.Chlamydia pneumoniae—induced macrophage foam cell formation is mediated by Toll-like receptor 2.Infect Immun,2007,75:753-759.
[9]Schaffner T,Taylor K,Bartucci EJ,et al.Arterial foam cells with distinctive immunomorphologic and histochemical features of macrophages.Am J Pathol,1980,100:57-80.
[10]Hansson GK.Atherosclerosis—an immune disease:The Anitschkov Lecture 2007.Atherosclerosis,2009,202:2-10.
[11]李嵘娟,杨娅,谢谨捷,等.超声生物显微镜在Apo E基因敲除小鼠动脉粥样硬化病变与C反应蛋白的相关性研究.心肺血管病杂志,2014,33:433-436+452.
[12]Goodnough LT,Kleinhenz ME,Goldsmith GH Jr,et al.Bovine aortic endothelial cells elaborate an inhibitor of the generation of lipopolysaccharide-stimulated human blood monocyte procoagulant activity.J Clin Invest,1984,74:75-81.
[13]Pomerantz RJ,Feinberg MB,Trono D,et al.Lipopolysaccharide is a potent monocyte/macrophage-specific stimulator of human immunodeficiency virus type 1 expression.J Exp Med,1990,172:253-261.
[14]Diya Zhang,Lili Chen,Shenglai Li,et al.Lipopolysaccharide(LPS)of Porphyromonas gingivalis induces IL-1beta,TNF-alpha and IL-6 production by THP-1 cells in a way different from that of Escherichia coli LPS.Innate Immun,2008,14:99-107.
[15]冯俊明,史景泉,刘友生.脂多糖对枯否细胞中CD14、To LL样受体4表达的影响及其意义.中华实验外科杂志,2002,19:350-351.
[16]黄秀榕,祁明信,王军.LPS对晶状体上皮细胞TLR4和CD14mRNA表达的影响.中国病理生理杂志,2010,26:797-801.
[17]侯本祥,张琛,荫俊.脂多糖对THP-1细胞CD14诱导表达的影响.首都医科大学学报,2005,26:711-713.
[18]叶强,雷寒,郑文武,等.脂多糖通过LDLr诱导巨噬细胞泡沫化:炎症应激下巨噬细胞泡沫化的另一条通路.中国药理学通报,2013,29:1064-1070.
[19]叶强,雷寒,范忠才,等.炎症致巨噬细胞和血管平滑肌细胞低密度脂蛋白受体反馈调控差异的研究.检验医学与临床,2014,11:2193-2198.
[2]Tang WH,Hazen SL.The contributory role of gut microbiota in cardiovascular disease.J Clin Invest,2014,124:4204-4211.
[3]Serino M,Blasco-Baque V,Nicolas S,et al.Far from the Eyes,Close to the Heart:Dysbiosis of Gut Microbiota and Cardiovascular Consequences.Curr Cardiol Rep,2014,16:540.
[4]李坑,朱光发.肠道菌群与代谢综合征.心肺血管病杂志,2014,33:742-744.
[5]Koren O,Spor A,Felin J,et al.Human oral,gut,and plaque microbiota in patients with atherosclerosis.Proc Natl Acad Sci U S A,2011,108(Suppl 1):4592-4598.
[6]Sekine K,Ohta J,Onishi M,et al.Analysis of antitumor properties of effector cells stimulated with a cell wall preparation(WPG)of Bifidobacterium infantis.Biol Pharm Bull,1995,18:148-153.
[7]许建华,段光杰,朱江,等.分化诱导剂PMA对THP-1源性巨噬细胞膜表面受体MD-2表达的影响.免疫学杂志,2013,29:121-125.
[8]Cao F,Castrillo A,Tontonoz P,et al.Chlamydia pneumoniae—induced macrophage foam cell formation is mediated by Toll-like receptor 2.Infect Immun,2007,75:753-759.
[9]Schaffner T,Taylor K,Bartucci EJ,et al.Arterial foam cells with distinctive immunomorphologic and histochemical features of macrophages.Am J Pathol,1980,100:57-80.
[10]Hansson GK.Atherosclerosis—an immune disease:The Anitschkov Lecture 2007.Atherosclerosis,2009,202:2-10.
[11]李嵘娟,杨娅,谢谨捷,等.超声生物显微镜在Apo E基因敲除小鼠动脉粥样硬化病变与C反应蛋白的相关性研究.心肺血管病杂志,2014,33:433-436+452.
[12]Goodnough LT,Kleinhenz ME,Goldsmith GH Jr,et al.Bovine aortic endothelial cells elaborate an inhibitor of the generation of lipopolysaccharide-stimulated human blood monocyte procoagulant activity.J Clin Invest,1984,74:75-81.
[13]Pomerantz RJ,Feinberg MB,Trono D,et al.Lipopolysaccharide is a potent monocyte/macrophage-specific stimulator of human immunodeficiency virus type 1 expression.J Exp Med,1990,172:253-261.
[14]Diya Zhang,Lili Chen,Shenglai Li,et al.Lipopolysaccharide(LPS)of Porphyromonas gingivalis induces IL-1beta,TNF-alpha and IL-6 production by THP-1 cells in a way different from that of Escherichia coli LPS.Innate Immun,2008,14:99-107.
[15]冯俊明,史景泉,刘友生.脂多糖对枯否细胞中CD14、To LL样受体4表达的影响及其意义.中华实验外科杂志,2002,19:350-351.
[16]黄秀榕,祁明信,王军.LPS对晶状体上皮细胞TLR4和CD14mRNA表达的影响.中国病理生理杂志,2010,26:797-801.
[17]侯本祥,张琛,荫俊.脂多糖对THP-1细胞CD14诱导表达的影响.首都医科大学学报,2005,26:711-713.
[18]叶强,雷寒,郑文武,等.脂多糖通过LDLr诱导巨噬细胞泡沫化:炎症应激下巨噬细胞泡沫化的另一条通路.中国药理学通报,2013,29:1064-1070.
[19]叶强,雷寒,范忠才,等.炎症致巨噬细胞和血管平滑肌细胞低密度脂蛋白受体反馈调控差异的研究.检验医学与临床,2014,11:2193-2198.