还原型谷胱甘肽对大菱鲆生长及抗氧化能力的影响
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摘要
在基础饲料中添加不同剂量的还原型谷胱甘肽,添加量分别为0、100、200、400、600 mg/kg,投喂初始体质量为(23.08±0.09) g的大菱鲆,8周后测定还原型谷胱甘肽对大菱鲆生长及抗氧化能力的影响。试验结果表明,饲料中还原型谷胱甘肽添加量为200 mg/kg时,大菱鲆的质量增加率和特定生长率显著高于其他组(P<0.05)。饲料中添加还原型谷胱甘肽对大菱鲆肝脏中丙二醛含量、总抗氧化能力、超氧化物歧化酶活力、谷胱甘肽过氧化物酶活力均无显著影响(P>0.05)。随着还原型谷胱甘肽添加量的增加,大菱鲆肝脏中丙二醛含量呈先降后升的趋势,对照组最高,200 mg/kg试验组最低;大菱鲆肝脏中总抗氧化能力、超氧化物歧化酶活力和谷胱甘肽过氧化物酶活力均呈先升后降的趋势,200 mg/kg试验组最高。200 mg/kg试验组和400 mg/kg试验组大菱鲆肝脏中还原型谷胱甘肽含量显著高于对照组(P<0.05)。200 mg/kg试验组大菱鲆肝脏中谷胱甘肽硫转移酶活力和谷胱甘肽还原酶活力显著低于对照组(P<0.05)。根据回归分析,确定大菱鲆饲料中还原型谷胱甘肽的最适添加量为189.70 mg/kg。
An 8-week feeding trial was conducted to evaluate effects of dietary glutathione(GSH) on growth performance and antioxidation capacity of turbot Scophthalmus maximus. The turbot with initial weight of(23.08±0.09) g were fed the basal diet supplemented with 0, 100, 200, 400, and 600 mg/kg reduced glutathione(GSH), respectively, at water temperature of(14.0—18.5) ℃. The results showed that weight gain rate and specific growth rate were significantly higher in the turbot in 200 mg/kg dietary GSH group than those in the other groups(P<0.05). There were no significant differences in the malondialdehyde(MDA) level, total antioxidant capacity(T-AOC), superoxide dismutase(SOD) activity and glutathione peroxidase(GSH-PX) activity in the liver of turbot(P>0.05). With the increase of the dietary GSH, there was the maximal MDA level in the liver of turbot in the control group, and the minimal in the turbot in the 200 mg/kg GSH group. All the T-AOC, SOD activity and GSH-PX activity in the liver were shown to be a trend of increasing at first and then decreasing, the maximum in the 200 mg/kg dietary GSH group. In the 200 mg/kg and 400 mg/kg dietary GSH groups, the GSH of the liver of turbot was significantly higher than that in the control group(P<0.05). In the 200 mg/kg dietary GSH group, the GST activity and the GR activity in the liver was significantly lower than that in the control group(P<0.05). The regression analysis revealed that the appropriate level of dietary GSH was 189.70 mg/kg.
An 8-week feeding trial was conducted to evaluate effects of dietary glutathione(GSH) on growth performance and antioxidation capacity of turbot Scophthalmus maximus. The turbot with initial weight of(23.08±0.09) g were fed the basal diet supplemented with 0, 100, 200, 400, and 600 mg/kg reduced glutathione(GSH), respectively, at water temperature of(14.0—18.5) ℃. The results showed that weight gain rate and specific growth rate were significantly higher in the turbot in 200 mg/kg dietary GSH group than those in the other groups(P<0.05). There were no significant differences in the malondialdehyde(MDA) level, total antioxidant capacity(T-AOC), superoxide dismutase(SOD) activity and glutathione peroxidase(GSH-PX) activity in the liver of turbot(P>0.05). With the increase of the dietary GSH, there was the maximal MDA level in the liver of turbot in the control group, and the minimal in the turbot in the 200 mg/kg GSH group. All the T-AOC, SOD activity and GSH-PX activity in the liver were shown to be a trend of increasing at first and then decreasing, the maximum in the 200 mg/kg dietary GSH group. In the 200 mg/kg and 400 mg/kg dietary GSH groups, the GSH of the liver of turbot was significantly higher than that in the control group(P<0.05). In the 200 mg/kg dietary GSH group, the GST activity and the GR activity in the liver was significantly lower than that in the control group(P<0.05). The regression analysis revealed that the appropriate level of dietary GSH was 189.70 mg/kg.
引文
[1] 雷霁霖.大菱鲆的引进和新型养殖产业的发展[J].中国渔业经济,2003(5):16.
[2] 雷霁霖,刘新富,关长涛.中国大菱鲆养殖20年成就和展望——庆祝大菱鲆引进中国20周年[J].渔业科学进展,2012,33(4):123-130.
[3] 麦康森,艾庆辉,徐玮,等.水产养殖中的环境胁迫及其预防——营养学途径[J].中国海洋大学学报:自然科学版,2004,34(5):767-774.
[4] 牛化欣,雷霁霖,常杰,等.冰鲜野杂鱼和商品饲料对大菱鲆生长、脂质代谢及抗氧化功能的影响[J].动物营养学报,2013,25(11):2696-2704.
[5] 陈平洁,曹俊明,陈水春,等.水产养殖中的拥挤胁迫和操作胁迫[J].饲料工业,2007,28(22):18-21.
[6] 苗启庆.谷胱甘肽的生理功能及其在食品加工中的应用前景[J].科技风,2010(8):253.
[7] Viňa J,Perez C,Furukawa T,et al.Effect of oral glutathione on hepatic glutathione levels in rats and mice[J].British Journal of Nutrition,1989,62(3):683-691.
[8] Ueno Y,Kizaki M,Nakagiri R,et al.Dietary glutathione protects rats from diabetic nephropathy and neuropathy[J].Journal of Nutrition,2002,132(5):897-900.
[9] 周婷婷.谷胱甘肽对吉富罗非鱼生长性能和抗氧化功能的影响[D].武汉:华中农业大学,2012.
[10] 王艺,孙国祥,刘鹰,等.日粮中添加谷胱甘肽对虹鳟生长性能和抗氧化性能的影响[J].大连海洋大学学报,2017,32(6):651-657.
[11] 刘晓华,曹俊明,吴建开,等.饲料中添加谷胱甘肽对凡纳滨对虾肝胰腺抗氧化指标和脂质过氧化物含量的影响[J].水产学报,2007,31(2):235-240.
[12] 张国良,赵会宏,周志伟,等.还原型谷胱甘肽对罗非鱼生长和抗氧化性能的影响[J].华南农业大学学报,2007,28(3):90-93.
[13] 王芳倩,张文兵,麦康森,等.饲料中添加还原型谷胱甘肽对牙鲆生长和抗氧化能力的影响[J].中国海洋大学学报:自然科学版,2011,41(4):51-56.
[14] 朱选,曹俊明,赵红霞,等.饲料中添加谷胱甘肽对草鱼组织中谷胱甘肽沉积和抗氧化能力的影响[J].中国水产科学,2008,15(1):160-166.
[15] 陈齐勇.饲料中α-硫辛酸、谷胱甘肽和硒对皱纹盘鲍(Haliotis discus hannai Ino)生长和抗氧化反应的影响[D].青岛:中国海洋大学,2010.
[16] 周婷婷,曹俊明,赵红霞,等.谷胱甘肽在水产动物中的营养生理作用研究进展[J].水产科学,2012,31(10):630-634.
[17] 赵红霞,谭永刚,周萌,等.饲料中添加谷胱甘肽对草鱼生长、生理指标和抗病力的影响[J].中国水产科学,2007,14(4):678-683.
[18] 周艳玲,孙育平,曹俊明,等.饲料中添加谷胱甘肽对黄颡鱼幼鱼生长性能、体成分、血清生化指标和抗氨氮应激能力的影响[J].动物营养学报,2017,29(11):4189-4197.
[19] 焦彩虹.谷胱甘肽对罗非鱼生长的影响及其机理研究[D].广州:华南农业大学,2004.
[20] Aw T Y,Williams M W,Gray L.Absorption and lymphatic transport of peroxidized lipids by rat small intestine in vivo:role of mucosal GSH[J].American Journal of Physiology,1992,262(1):99-106.
[21] Venturini G.The hydra GSH receptor.Pharmacological and radioligand binding studies[J].Comparative Biochemistry & Physiology Part C Comparative Pharmacology,1987,87(2):321-324.
[22] Almeida E A,Bainy A C D,Dafre A L,et al.Oxidative stress in digestive gland and gill of the brown mussel (Perna perna) exposed to air and re-submersed[J].Journal of Experimental Marine Biology & Ecology,2005,318(1):21-30.
[23] 周婷婷,曹俊明,黄燕华,等.饲料中添加谷胱甘肽对吉富罗非鱼生长、组织生化指标和非特异性免疫相关酶的影响[J].水产学报,2013,37(5):742-750.
[24] 马森.谷胱甘肽过氧化物酶和谷胱甘肽转硫酶研究进展[J].动物医学进展,2008,29(10):53-56.
[25] Bell J G,Adron J W,Cowey C B.Effect of selenium deficiency on hydroperoxide-stimulated release of glutathione from isolated perfused liver of rainbow trout (Salmo gairdneri)[J].British Journal of Nutrition,1986,56(2):421.
[26] Thomas S,Lowe J E,Hadjivassiliou V,et al.Use of the Comet assay to investigate the role of superoxide in glutathione-induced DNA damage[J].Biochem Biophys Res Commun,1998,243(1):241-245.
[27] 高姝娟,刘东波,罗贵民,等.抗氧化剂抗脂质过氧化机制的ESR研究[J].波谱学杂志,1998(2):139-143.
[28] Monks T J,Anders M W,Dekant W,et al.Glutathione conjugate mediated toxicities[J].Toxicology & Applied Pharmacology,1990,106(1):1-19.
[29] 何芬.谷胱甘肽和蛋白酶解物对仔稚鱼生长及营养生理的影响[D].广州:华南农业大学,2006.
[30] 梁春梅.还原型谷胱甘肽对奥尼罗非鱼(Oreochromis niloticus×O.aureus)幼鱼生长、免疫功能的影响及其机理研究[D].广州:华南农业大学,2006.
[2] 雷霁霖,刘新富,关长涛.中国大菱鲆养殖20年成就和展望——庆祝大菱鲆引进中国20周年[J].渔业科学进展,2012,33(4):123-130.
[3] 麦康森,艾庆辉,徐玮,等.水产养殖中的环境胁迫及其预防——营养学途径[J].中国海洋大学学报:自然科学版,2004,34(5):767-774.
[4] 牛化欣,雷霁霖,常杰,等.冰鲜野杂鱼和商品饲料对大菱鲆生长、脂质代谢及抗氧化功能的影响[J].动物营养学报,2013,25(11):2696-2704.
[5] 陈平洁,曹俊明,陈水春,等.水产养殖中的拥挤胁迫和操作胁迫[J].饲料工业,2007,28(22):18-21.
[6] 苗启庆.谷胱甘肽的生理功能及其在食品加工中的应用前景[J].科技风,2010(8):253.
[7] Viňa J,Perez C,Furukawa T,et al.Effect of oral glutathione on hepatic glutathione levels in rats and mice[J].British Journal of Nutrition,1989,62(3):683-691.
[8] Ueno Y,Kizaki M,Nakagiri R,et al.Dietary glutathione protects rats from diabetic nephropathy and neuropathy[J].Journal of Nutrition,2002,132(5):897-900.
[9] 周婷婷.谷胱甘肽对吉富罗非鱼生长性能和抗氧化功能的影响[D].武汉:华中农业大学,2012.
[10] 王艺,孙国祥,刘鹰,等.日粮中添加谷胱甘肽对虹鳟生长性能和抗氧化性能的影响[J].大连海洋大学学报,2017,32(6):651-657.
[11] 刘晓华,曹俊明,吴建开,等.饲料中添加谷胱甘肽对凡纳滨对虾肝胰腺抗氧化指标和脂质过氧化物含量的影响[J].水产学报,2007,31(2):235-240.
[12] 张国良,赵会宏,周志伟,等.还原型谷胱甘肽对罗非鱼生长和抗氧化性能的影响[J].华南农业大学学报,2007,28(3):90-93.
[13] 王芳倩,张文兵,麦康森,等.饲料中添加还原型谷胱甘肽对牙鲆生长和抗氧化能力的影响[J].中国海洋大学学报:自然科学版,2011,41(4):51-56.
[14] 朱选,曹俊明,赵红霞,等.饲料中添加谷胱甘肽对草鱼组织中谷胱甘肽沉积和抗氧化能力的影响[J].中国水产科学,2008,15(1):160-166.
[15] 陈齐勇.饲料中α-硫辛酸、谷胱甘肽和硒对皱纹盘鲍(Haliotis discus hannai Ino)生长和抗氧化反应的影响[D].青岛:中国海洋大学,2010.
[16] 周婷婷,曹俊明,赵红霞,等.谷胱甘肽在水产动物中的营养生理作用研究进展[J].水产科学,2012,31(10):630-634.
[17] 赵红霞,谭永刚,周萌,等.饲料中添加谷胱甘肽对草鱼生长、生理指标和抗病力的影响[J].中国水产科学,2007,14(4):678-683.
[18] 周艳玲,孙育平,曹俊明,等.饲料中添加谷胱甘肽对黄颡鱼幼鱼生长性能、体成分、血清生化指标和抗氨氮应激能力的影响[J].动物营养学报,2017,29(11):4189-4197.
[19] 焦彩虹.谷胱甘肽对罗非鱼生长的影响及其机理研究[D].广州:华南农业大学,2004.
[20] Aw T Y,Williams M W,Gray L.Absorption and lymphatic transport of peroxidized lipids by rat small intestine in vivo:role of mucosal GSH[J].American Journal of Physiology,1992,262(1):99-106.
[21] Venturini G.The hydra GSH receptor.Pharmacological and radioligand binding studies[J].Comparative Biochemistry & Physiology Part C Comparative Pharmacology,1987,87(2):321-324.
[22] Almeida E A,Bainy A C D,Dafre A L,et al.Oxidative stress in digestive gland and gill of the brown mussel (Perna perna) exposed to air and re-submersed[J].Journal of Experimental Marine Biology & Ecology,2005,318(1):21-30.
[23] 周婷婷,曹俊明,黄燕华,等.饲料中添加谷胱甘肽对吉富罗非鱼生长、组织生化指标和非特异性免疫相关酶的影响[J].水产学报,2013,37(5):742-750.
[24] 马森.谷胱甘肽过氧化物酶和谷胱甘肽转硫酶研究进展[J].动物医学进展,2008,29(10):53-56.
[25] Bell J G,Adron J W,Cowey C B.Effect of selenium deficiency on hydroperoxide-stimulated release of glutathione from isolated perfused liver of rainbow trout (Salmo gairdneri)[J].British Journal of Nutrition,1986,56(2):421.
[26] Thomas S,Lowe J E,Hadjivassiliou V,et al.Use of the Comet assay to investigate the role of superoxide in glutathione-induced DNA damage[J].Biochem Biophys Res Commun,1998,243(1):241-245.
[27] 高姝娟,刘东波,罗贵民,等.抗氧化剂抗脂质过氧化机制的ESR研究[J].波谱学杂志,1998(2):139-143.
[28] Monks T J,Anders M W,Dekant W,et al.Glutathione conjugate mediated toxicities[J].Toxicology & Applied Pharmacology,1990,106(1):1-19.
[29] 何芬.谷胱甘肽和蛋白酶解物对仔稚鱼生长及营养生理的影响[D].广州:华南农业大学,2006.
[30] 梁春梅.还原型谷胱甘肽对奥尼罗非鱼(Oreochromis niloticus×O.aureus)幼鱼生长、免疫功能的影响及其机理研究[D].广州:华南农业大学,2006.