柴胡皂苷对癫痫大鼠皮质和海马区谷氨酸、γ-羟基丁酸含量的影响
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摘要
目的观察柴胡皂苷对癫痫大鼠皮质和海马区谷氨酸、γ-羟基丁酸含量调节的影响。方法将健康SD大鼠给予反复腹腔注射氯化锂-匹罗卡品化学点燃建立大鼠癫痫的动物模型,将制备成功的癫痫大鼠模型随机分为模型对照组及柴胡皂苷组,各15只,另选择15只正常大鼠作为正常对照组。柴胡皂苷组予以腹腔注射柴胡皂苷悬浊液1.09 mL/(kg·d),模型对照组及正常对照组给予等量0.9%氯化钠,使用方法、剂量及使用频率和柴胡皂苷组相同至实验结束。比较各组大鼠癫痫发作情况、皮质和海马区谷氨酸和γ-羟基丁酸含量。结果治疗后4周、8周,与模型对照组比较,柴胡皂苷组癫痫发作次数减少、发作时间短,且柴胡皂苷组发作次数、发作时间持续降低(P <0.05)。与正常对照组比较,模型对照组和柴胡皂苷组皮质和海马区谷氨酸阳性细胞数较高(P <0.05);与模型对照组比较,柴胡皂苷组皮质和海马区谷氨酸阳性细胞数较少(P <0.05)。治疗后,与正常对照组比较,模型对照组及柴胡皂苷组皮质和海马区γ-羟基丁酸阳性细胞数较少(P <0.05);与模型对照组比较,柴胡皂苷组皮质和海马区γ-羟基丁酸阳性细胞数较多(P <0.05)。结论柴胡皂苷可降低癫痫大鼠皮质和海马脑区兴奋性氨基酸谷氨酸水平,减缓病变神经元兴奋性,起到抗癫痫作用,提高γ-羟基丁酸含量,降低神经元兴奋性,抑制癫痫发作,缓解癫痫发作所致的神经系统受损,具有显著抑制癫痫发作、保护神经元作用。
Objective To investigate the effects of Radix Bupleuri(RB)on the contents of glutamate and gamma-aminobutyric acid(GABA)in cortex and hippocampus of epileptic rats.Methods Healthy Sprague-Dawley(SD)rats were given repeated intraperitoneal injection of lithium pilocarpine kindling to establish the rat models of epilepsy.The model rats were randomly divided into model control group(n=15)and RB group(n=15),and 15 normal rats were selected as normal control group.The rats in RB group were received intraperitoneal injection of RB1.09 mL/(kg·d).The rats in control group and the normal control group were given the same amount of 0.9% sodium chloride.The epileptic seizures,the contents of glutamate,and GABA in cortex and hippocampus of rats in each group were compared.Results After treatment for 4 weeks and 8 weeks,compared with the model control group,the numbers of seizures and the average time of onset in RB group were lower,and the numbers of seizures and the average duration of the onset were decreased(P <0.05).Compared with the normal control group,the number of glutamate positive cells in the cortex and hippocampus were higher in the model group and RB group(P <0.05).Compared with the model group,the number of glutamate positive cells in the cortex and hippocampus was lower in RB group(P <0.05).After treatment,compared with the normal control group,GABA positive cells in cortex and hippocampus was lower in model control group and RB group(P <0.05).Compared with the model group,GABA positive cell count was higher in cortex and hippocampus of RB group(P <0.05).Conclusion RB can reduce the level of excitatory amino acid glutamate in the cortex and hippocampus of rats with epilepsy,slow down the excitability of diseased neurons,play an anti-epileptic effect,increase the content ofγ-hydroxybutyric acid,reduce the excitability of neurons,and inhibit epilepsy attack,relieve the damage of the nervous system caused by seizures inhibit of seizures and protect neurons.
Objective To investigate the effects of Radix Bupleuri(RB)on the contents of glutamate and gamma-aminobutyric acid(GABA)in cortex and hippocampus of epileptic rats.Methods Healthy Sprague-Dawley(SD)rats were given repeated intraperitoneal injection of lithium pilocarpine kindling to establish the rat models of epilepsy.The model rats were randomly divided into model control group(n=15)and RB group(n=15),and 15 normal rats were selected as normal control group.The rats in RB group were received intraperitoneal injection of RB1.09 mL/(kg·d).The rats in control group and the normal control group were given the same amount of 0.9% sodium chloride.The epileptic seizures,the contents of glutamate,and GABA in cortex and hippocampus of rats in each group were compared.Results After treatment for 4 weeks and 8 weeks,compared with the model control group,the numbers of seizures and the average time of onset in RB group were lower,and the numbers of seizures and the average duration of the onset were decreased(P <0.05).Compared with the normal control group,the number of glutamate positive cells in the cortex and hippocampus were higher in the model group and RB group(P <0.05).Compared with the model group,the number of glutamate positive cells in the cortex and hippocampus was lower in RB group(P <0.05).After treatment,compared with the normal control group,GABA positive cells in cortex and hippocampus was lower in model control group and RB group(P <0.05).Compared with the model group,GABA positive cell count was higher in cortex and hippocampus of RB group(P <0.05).Conclusion RB can reduce the level of excitatory amino acid glutamate in the cortex and hippocampus of rats with epilepsy,slow down the excitability of diseased neurons,play an anti-epileptic effect,increase the content ofγ-hydroxybutyric acid,reduce the excitability of neurons,and inhibit epilepsy attack,relieve the damage of the nervous system caused by seizures inhibit of seizures and protect neurons.
引文
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[13]黄运生,卓鹰,刘智良.柴胡疏肝汤对戊四氮致痫大鼠海马及额叶皮质bcl-2、bax表达的影响[J].中华临床医师杂志:电子版,2012,6(6):1574-1575.
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[15]徐元,包仕尧,张志琳,等.癫痫大鼠海马细胞外液中γ-氨基丁酸、谷氨酸、牛磺酸及甘氨酸的动态变化[J].苏州大学学报:医学版,2005,25(3):370-372.
[16]罗鑫,张骏,徐祖才.神经系统疾病中兴奋性氨基酸作用的研究进展[J].癫痫与神经电生理学杂志,2015,24(5):307-310.
[17]潘莉.谷氨酸在缺血性脑损伤中的作用及机理研究概述[J].右江民族医学院学报,2007,29(5):835-837.
[18]唐太峰,伍国锋.低频电刺激对耐药癫痫大鼠海马细胞外液谷氨酸及γ-氨基丁酸的影响[J].贵阳医学院学报,2016,41(7):793-796.
[19]程莉晶,朱雨岚.细胞因子中白细胞介素1β、白细胞介素1Ra及γ-氨基丁酸B受体与癫痫的发病[J].中国组织工程研究,2007,11(27):5403-5406.
[2]王文志,吴建中,王德生,等.中国五省农村人群癫痫流行病学抽样调查[J].中华医学杂志,2002,82(7):449-452.
[3]张世忠.关于癫痫外科治疗的几点思考[J].中华神经外科杂志,2010,26(6):481-482.
[4]邓元江,刘卫英,梁伟雄,等.针刺对急性癫痫大鼠脑电功率及海马氨基酸类递质的影响[J].广州中医药大学学报,2006,23(6):509-512.
[5]王群,阮旭中,李震中,等.贝美格致痫大鼠皮层、海马γ-氨基丁酸和谷氨酸免疫反应细胞的改变[J].中风与神经疾病,2001,18(4):215-217.
[6]富瑶瑶,安家彦,鱼红闪,等.特异的柴胡皂苷糖苷酶的分离纯化[J].高等学校化学学报,2010,31(6):1152-1157.
[7]李新民,秦潞平,路岩莉.熄风胶囊对氯化锂-匹罗卡品癫痫大鼠反复自发性发作及海马损伤的干预作用[J].中国中西医结合儿科学,2012,4(6):481-484.
[8]王婧婧,赵卫,孙学进.原发性癫痫的功能磁共振成像研究进展[J].中国医学影像学杂志,2016,24(9):714-716.
[9]程记伟,白宇.癫痫的药物治疗进展[J].现代医药卫生,2008,24(4):540-541.
[10]冯占辉,晏勇.癫痫患者脑脊液中相关生物学标志物的研究进展[J].中国神经精神疾病杂志,2008,34(10):639-640.
[11]中华医学会神经病学分会脑电图与癫痫学组.抗癫痫药物应用专家共识[J].中华神经科杂志,2011,44(1):20-21.
[12]赵秀欣,张微微,郭文华,等.长期服用抗癫痫药物对原发性癫痫患者平均血红蛋白含量的影响[J].临床军医杂志,2010,38(2):252-253.
[13]黄运生,卓鹰,刘智良.柴胡疏肝汤对戊四氮致痫大鼠海马及额叶皮质bcl-2、bax表达的影响[J].中华临床医师杂志:电子版,2012,6(6):1574-1575.
[14]谢炜,鲍勇.柴胡皂苷a对戊四氮诱导小鼠痫性发作的影响[J].安徽中医药大学学报,2006,25(1):24-26.
[15]徐元,包仕尧,张志琳,等.癫痫大鼠海马细胞外液中γ-氨基丁酸、谷氨酸、牛磺酸及甘氨酸的动态变化[J].苏州大学学报:医学版,2005,25(3):370-372.
[16]罗鑫,张骏,徐祖才.神经系统疾病中兴奋性氨基酸作用的研究进展[J].癫痫与神经电生理学杂志,2015,24(5):307-310.
[17]潘莉.谷氨酸在缺血性脑损伤中的作用及机理研究概述[J].右江民族医学院学报,2007,29(5):835-837.
[18]唐太峰,伍国锋.低频电刺激对耐药癫痫大鼠海马细胞外液谷氨酸及γ-氨基丁酸的影响[J].贵阳医学院学报,2016,41(7):793-796.
[19]程莉晶,朱雨岚.细胞因子中白细胞介素1β、白细胞介素1Ra及γ-氨基丁酸B受体与癫痫的发病[J].中国组织工程研究,2007,11(27):5403-5406.