氟暴露小鼠睾丸形态计量学研究
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摘要
为了观察小鼠氟中毒睾丸形态变化特点,研究氟对睾丸的毒性影响,选用45只健康性成熟雄性昆明系小鼠,随机分为对照组、低氟组(25 mg/L Na F)、高氟组(100 mg/L Na F),连续饲喂60 d,制作睾丸石蜡组织切片,H.E.染色后,显微镜下观察曲细精管形态变化,通过定量分析方法探讨氟对睾丸的毒性影响。结果表明,与对照组相比,低氟组和高氟组曲细精管半径、面积以及曲细精管管腔半径、面积均无显著性差异(P>0.05);高氟组小鼠曲细精管细胞层数和厚度极明显低于对照组(P<0.01);形态学观察显示,25 mg/L氟中毒小鼠睾丸生精细胞减少,100 mg/L Na F可导致生精细胞排列紊乱、各级生精细胞减少等。本试验可为氟的雄性生殖毒性研究提供直观、科学的参考。
引文
[1]王俊东.氟中毒研究[M].北京:中国农业出版社,2007.
[2]Doull J,Boekelheide K,Farishian B G,et al.Fluoride in Drink-ing Water:A Scientific Review of EPA’s Standards[M].Washing-ton:National Academies.2006.
[3]Freni S C.Exposure to high fluoride concentrations in drinkingwater is associated with decreased birth rates[J].J Toxicol EnvironHealth,1994,42(1):109-121.
[4]Long H,Jin Y,Lin M,et al.Fluoride toxicity in the male repro-ductive system[J].Fluoride,2009,42(4):260-272.
[5]孙子龙,牛瑞燕,王俊东.氟对雄性小鼠生长发育及性腺中氟含量的影响[J].中国畜牧兽医,2012,39(3):227-229.
[6]Sarkar S D,Maiti R,Ghosh D.Management of fluoride inducedtesticular disorders by calcium and vitamin-E co-administrationin the albino rat[J].Reprod Toxicol,2006,22(4):606-612.
[7]肖跃海,孙发,李崇斌,等.燃煤型氟中毒对大鼠睾丸组织氧化应激效应的影响[J].中国医学科学院学报,2011,33(4):357-361.
[8]Wan S X,Zhang J H,Wang J D.Effects of high fluoride onsperm quality and testicular histology in male rats[J].Fluoride,2006,39(1):17-21.
[9]杨志强,王晓亚,曹鹏,等.氟对小鼠附睾中成熟精子超微结构的影响[J].中国畜牧兽医,2014,41(8):137-140.
[2]Doull J,Boekelheide K,Farishian B G,et al.Fluoride in Drink-ing Water:A Scientific Review of EPA’s Standards[M].Washing-ton:National Academies.2006.
[3]Freni S C.Exposure to high fluoride concentrations in drinkingwater is associated with decreased birth rates[J].J Toxicol EnvironHealth,1994,42(1):109-121.
[4]Long H,Jin Y,Lin M,et al.Fluoride toxicity in the male repro-ductive system[J].Fluoride,2009,42(4):260-272.
[5]孙子龙,牛瑞燕,王俊东.氟对雄性小鼠生长发育及性腺中氟含量的影响[J].中国畜牧兽医,2012,39(3):227-229.
[6]Sarkar S D,Maiti R,Ghosh D.Management of fluoride inducedtesticular disorders by calcium and vitamin-E co-administrationin the albino rat[J].Reprod Toxicol,2006,22(4):606-612.
[7]肖跃海,孙发,李崇斌,等.燃煤型氟中毒对大鼠睾丸组织氧化应激效应的影响[J].中国医学科学院学报,2011,33(4):357-361.
[8]Wan S X,Zhang J H,Wang J D.Effects of high fluoride onsperm quality and testicular histology in male rats[J].Fluoride,2006,39(1):17-21.
[9]杨志强,王晓亚,曹鹏,等.氟对小鼠附睾中成熟精子超微结构的影响[J].中国畜牧兽医,2014,41(8):137-140.