陆地棉GhDHN1基因结构及内含子生物信息学分析
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摘要
利用生物信息学方法分析陆地棉脱水素基因GhDHN1的DNA序列结构特性,预测其功能。序列分析表明,GhDHN1基因的基因组DNA中AT碱基含量为52.16%,而内含子具有较高的AT碱基含量(63.33%);GhDHN1基因不含CpG岛;其内含子序列含有核心启动子元件TATA框、启动子的增强子元件CAAT框,并含有参与光响应的保守DNA模块的部分元件(Box4)、赤霉素响应元件GARE-motif、生长素响应元件TGA元件等,说明该内含子可能参与了该基因的转录活动,可能受光、激素的诱导;GhDHN1内含子具有GT-AG规则的保守序列,推测GhDHN1基因具有保守的内含子剪接机制。
引文
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[2]Wahid A,Close T J.Expression of dehydrins under heat stress and their relationship with water relations of sugarcane leaves[J].Biologia Plantarum,2007,51(1):104-109.
[3]Shinozaki K,Yamaguchi-Shinozaki K,Shinozaki K,et al.Gene networks involved in drought stress response and tolerance[J].Journal of Experimental Botany,2007,58(2):221-227.
[4]王俊娟,穆敏,王帅,等.棉花脱水素Gh DHN1的克隆及其表达[J].中国农业科学,2016,49(15):2867-2878.
[5]王俊娟,王德龙,王帅,等.陆地棉脱水素基因Gh DHN1启动子序列分析[J].中国棉花,2017,44(5):10-12.
[6]陈兵,文建凡.内含子在生物信息学研究和基因工程中的应用[J].生命的化学,2010,30(1):59-63.
[7]Nelsen N S,Marcotte W R,Jr.A wheat group 1 Lea intron enhancesβ-glucuronidase gene expression in cereal cells[J].Journal of Plant Physiology,2000,157(6):677-684.
[8]Li Fuguang,Fan Guangyi,Lu Cairui,et al.Genome sequence of cultivated upland cotton(Gossypium hirsutum TM-1)provides insights into genome evolution[J].Nature Biotechnology,2015,33(5):524-530.
[9]冉昆,王少敏.Windows 7平台下BLAST本地化构建[J].落叶果树,2015,47(3):39-42.
[10]王俊娟,阴祖军,王德龙,等.陆地棉脱水素蛋白Gh DHN1的结构特征和无序性分析[J].中国棉花,2017,44(8):17-19.
[11]孙乃恩,孙东旭,朱德煦.分子遗传学[M].南京:南京大学出版社,1990:7.
[12]杨寅桂,娄群峰,陈劲枫,等.黄瓜CSHSP70基因内含子结构分析[J].中国农业科学,2007,40(12):2915-2919.
[13]Jiménez-Bremont J F,Maruri-López I,Ochoa-Alfaro A E,et al.LEA,gene introns:is the intron of dehydrin genes a characteristic of the serine-segment?[J].Plant Molecular Biology Reporter,2013,31(1):128-140.
[14]Salgueiro S,Pignocchi C,Parry M A J.Intron-mediated gus A expression in tritordeum and wheat resulting from particle bombardment[J].Plant Molecular Biology,2000,42(4):615-622.
[15]雷晨,刘胜利,于婷乔,等.沙冬青Am CIP基因结构及其内含子生物信息学分析[J].生物技术通报,2016,32(9):93-99.