日粮中铜水平对貉造血功能和肝脏铜浓度的影响
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摘要
为了分析日粮中不同水平的铜对貉造血功能和肝脏中铜的沉积量的影响,试验选取貉12只,随机平分成4组,分别编号为Ⅰ、Ⅱ、Ⅲ、Ⅳ组。Ⅳ组为对照组正常投喂基础日粮,基础日粮中铜为10 mg/kg。Ⅰ、Ⅱ、Ⅲ组为试验组,在基于日粮铜水平上分别添加10 mg/kg、20 mg/kg、30 mg/kg的外源铜。试验期为60 d。试验结果表明:Ⅰ、Ⅱ、Ⅲ组RBC(红细胞数)、HGB(血红蛋白数)、HCT(红细胞比容) 3项血液指标先上升,后逐渐稳定,高于Ⅳ组,且差异显著(P <0. 05);Ⅰ、Ⅱ组肝脏铜沉积量与日粮中铜含量呈正相关,Ⅲ组肝脏铜沉积量明显高于其他3组,且差异极显著(P <0. 01)。说明日粮中添加外源铜对貉的造血功能有促进作用;当日粮中铜水平超过30 mg/kg时铜会在貉肝脏中加倍沉积。
We investigated the effects of dietary copper levels on hematopoietic function and liver copper deposition in raccoon dog( Nyctereutes procyonoides). Twelve raccoon dogs were selected and randomly and evenly grouped into four groups of three( groups Ⅰ,Ⅱ,Ⅲ and Ⅳ). Group Ⅳserved as the control group which was fed a basic diet containing 10 mg/kg of copper. Experimental groups Ⅰ,Ⅱ,and Ⅲ were fed the basic diet plus 10 mg/kg,20 mg/kg and 30 mg/kg of external copper,respectively,for the 60-day trial. The results showed that the red blood cell( RBC),hemoglobin( HGB) and hematocrit( HCT) of groups Ⅰ,Ⅱ and Ⅲ increased and stabilized,and all were significantly higher than those of group Ⅳ( P < 0. 05). The liver copper deposition in groups Ⅰ and Ⅱ was positively correlated with dietary copper level,however that in groupⅢ was significantly higher than in other three groups( P < 0. 01). Thus,the hematopoietic functions of raccoon dog were promoted by the addition of external dietary copper. When the dietary copper level exceeded 30 mg/kg,copper deposition doubled in the liver of raccoon dog.
We investigated the effects of dietary copper levels on hematopoietic function and liver copper deposition in raccoon dog( Nyctereutes procyonoides). Twelve raccoon dogs were selected and randomly and evenly grouped into four groups of three( groups Ⅰ,Ⅱ,Ⅲ and Ⅳ). Group Ⅳserved as the control group which was fed a basic diet containing 10 mg/kg of copper. Experimental groups Ⅰ,Ⅱ,and Ⅲ were fed the basic diet plus 10 mg/kg,20 mg/kg and 30 mg/kg of external copper,respectively,for the 60-day trial. The results showed that the red blood cell( RBC),hemoglobin( HGB) and hematocrit( HCT) of groups Ⅰ,Ⅱ and Ⅲ increased and stabilized,and all were significantly higher than those of group Ⅳ( P < 0. 05). The liver copper deposition in groups Ⅰ and Ⅱ was positively correlated with dietary copper level,however that in groupⅢ was significantly higher than in other three groups( P < 0. 01). Thus,the hematopoietic functions of raccoon dog were promoted by the addition of external dietary copper. When the dietary copper level exceeded 30 mg/kg,copper deposition doubled in the liver of raccoon dog.
引文
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[13] HWANG D F,WANG L C,CHENG H M.Effect of taurine on toxicity of copper in rats[J].Food And Chemical Toxicology,1998,36(3):239-244.
[14] SUGAWARA N,LI D,SUGAWARA C,et al.Response of hepatic function to hepatic copper deposition in rats fed a diet containing copper[J].Biological Trace Element Research,1995,49(2/3):161.
[15] 王建明,孙奕南,谢璋琪.生长猪饲料添加中等剂量铜的可能性探讨[J].广东饲料,1999(3):17-18.
[16] AULERICH R J,RINGER R K,BLEAVINS M R,et al.Effects of supplemental dietary copper on growth,reproductive performance and kit survival of standard dark mink and the acute toxicity of copper to mink[J].Journal of Animal Science,1982,55(2):337-343.
[17] 陈蓉.貉子饲料中毒怎么办[J].农村实用技术,2008(2):52.
[18] 陈国发.貉子的饲料和饲养管理[J].养殖技术顾问,2014(6):85.
[19] BUSH C R,RESTUM J C,BURSIAN S J,et al.Responses of growing mink to supplemental dietary copper and biotin[J].Scientifur,1995,19(2):141-147.
[20] LóPEZ-SERRANO D,SOLANO F,SANCHEZ-AMAT A.Involvement of a novel copper chaperone in tyrosinase activity and melanin synthesis in Marinomonas mediterranea[J].Microbiology,2007,153(7):2241-2249.
[2] 雷鸣,隋啸一,刘磊,等.饲粮铜对生长獭兔血清免疫、血液生化、铜消化率及组织铜沉积的影响[J].山东畜牧兽医,2017(7):3-5.
[3] 李延鹏,张光超,马泽芳.饲粮高剂量铜添加水平对育成前期水貂生长发育及血清生化指标的影响[J].中国农学通报,2012,28(35):6-12.
[4] 陈龙,潘家强,唐兆新.高铜饲料对肉鸡肝脏和肌肉组织中铜残留的影响[J].黑龙江畜牧兽医(科技版),2012(19):67-68.
[5] 杨赵伟,闫玉杰,武孝丽,等.冀东地区貉子饲料中铜、锌、锰含量调查分析[J].饲料工业,2016,37(13):57-59.
[6] 李光玉,王凯英,赵靖波.毛皮动物矿物元素的需要[J].经济动物学报,2003,7(4):10-13.
[7] 梁金强,黄芝瑛,赖俊青,等.Beagle犬一天内血液学和血液生化指标的波动[J].实验动物与比较医学,2005,25(4):230-232.
[8] 谭志文,廖灵冲.浅谈血液常规检验在动物临床上的应用意义[J].畜牧兽医科技信息,2008(8):38-39.
[9] 金光耀.扎龙自然保护区丹顶鹤血液生化指标的建立及比较研究[D].哈尔滨:东北林业大学,2006.
[10] 肖向红,高中信,王丽萍.狼的血液学和血液生化学参考值[J].兽类学报,1998,18(1):34-41.
[11] 吴学壮,张铁涛,崔虎,等.饲粮添加铜水平对育成期水貂生长性能、营养物质消化率及氮代谢的影响[J].动物营养学报,2012,24(6):1078-1084.
[12] DOVE C R,HAYDON K D.The effect of copper addition to diets with various iron levels on the performance and hematology of weanling swine[J].Journal Of Animal Science,1991,69(5):2013-2019.
[13] HWANG D F,WANG L C,CHENG H M.Effect of taurine on toxicity of copper in rats[J].Food And Chemical Toxicology,1998,36(3):239-244.
[14] SUGAWARA N,LI D,SUGAWARA C,et al.Response of hepatic function to hepatic copper deposition in rats fed a diet containing copper[J].Biological Trace Element Research,1995,49(2/3):161.
[15] 王建明,孙奕南,谢璋琪.生长猪饲料添加中等剂量铜的可能性探讨[J].广东饲料,1999(3):17-18.
[16] AULERICH R J,RINGER R K,BLEAVINS M R,et al.Effects of supplemental dietary copper on growth,reproductive performance and kit survival of standard dark mink and the acute toxicity of copper to mink[J].Journal of Animal Science,1982,55(2):337-343.
[17] 陈蓉.貉子饲料中毒怎么办[J].农村实用技术,2008(2):52.
[18] 陈国发.貉子的饲料和饲养管理[J].养殖技术顾问,2014(6):85.
[19] BUSH C R,RESTUM J C,BURSIAN S J,et al.Responses of growing mink to supplemental dietary copper and biotin[J].Scientifur,1995,19(2):141-147.
[20] LóPEZ-SERRANO D,SOLANO F,SANCHEZ-AMAT A.Involvement of a novel copper chaperone in tyrosinase activity and melanin synthesis in Marinomonas mediterranea[J].Microbiology,2007,153(7):2241-2249.