麦冬皂苷B对人胃癌MGC-803细胞侵袭和迁移能力的影响
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摘要
目的:观察麦冬皂苷B(O P-B)对人胃癌M G C-803细胞侵袭和迁移能力的影响及其作用机制初探。方法:体外培养人胃癌MGC-803细胞,分别以0、0.1、1、5、10、15和20μmol/L的OP-B干预MGC-803细胞24、48h,利用CCK-8法观察OP-B对胃癌MGC-803细胞活性的影响;细胞侵袭实验和划痕实验检测OP-B(5、10、15μmol/L)作用MGC-803细胞24h后细胞的侵袭和迁移能力。蛋白免疫印迹法(WesternBlot)检测细胞中MMP-2、MMP-9蛋白水平的变化。结果:OP-B能明显抑制胃癌MGC-803细胞增殖(P<0.05),并抑制其侵袭和迁移(P<0.05),同时能降低其MMP-2、MMP-9蛋白表达水平(P<0.05)。结论:OP-B能够抑制人胃癌MGC-803细胞的侵袭和迁移能力,其作用机制可能与抑制MMP-2、MMP-9蛋白的表达有关。
Objective: To observe the effects of ophiopogonin B(OP-B) on the invasion and migration of human gastric cancer MGC-803 cells, and to explore the possible mechanism. Methods: MGC-803 cells were treated with different concentrations(0, 0.1, 1, 5, 10, 15 and 20μmol/L) of OP-B for 24 h and 48 h. CCK-8 was used to detect the inhibitory effect of OP-B on the proliferation inhibition rate of MGC-803 cells. Transwell and scratch test were used to detect the invasion and migration ability of MGC-803 cells after treated with OP-B(5, 10, 15μmol/L) for 24 h. Western Blot was used to detect the expression levels of MMP-2 and MMP-9. Results: OP-B significantly inhibited the proliferation of gastric cancer MGC-803 cells(P<0.05), and reduced the invasion and migration ability of MGC-803 cells(P<0.05). In addition, 15μmol/L OP-B could decrease the expression levels of MMP-2 and MMP-9 in MGC-803 cells(P<0.05). Conclusion: OP-B can inhibit the proliferation, invasion and migration ability of MGC-803 cells. The mechanism may be related to the inhibition of MMP-2 and MMP-9 protein expression.
Objective: To observe the effects of ophiopogonin B(OP-B) on the invasion and migration of human gastric cancer MGC-803 cells, and to explore the possible mechanism. Methods: MGC-803 cells were treated with different concentrations(0, 0.1, 1, 5, 10, 15 and 20μmol/L) of OP-B for 24 h and 48 h. CCK-8 was used to detect the inhibitory effect of OP-B on the proliferation inhibition rate of MGC-803 cells. Transwell and scratch test were used to detect the invasion and migration ability of MGC-803 cells after treated with OP-B(5, 10, 15μmol/L) for 24 h. Western Blot was used to detect the expression levels of MMP-2 and MMP-9. Results: OP-B significantly inhibited the proliferation of gastric cancer MGC-803 cells(P<0.05), and reduced the invasion and migration ability of MGC-803 cells(P<0.05). In addition, 15μmol/L OP-B could decrease the expression levels of MMP-2 and MMP-9 in MGC-803 cells(P<0.05). Conclusion: OP-B can inhibit the proliferation, invasion and migration ability of MGC-803 cells. The mechanism may be related to the inhibition of MMP-2 and MMP-9 protein expression.
引文
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[12]何冰倩,周小杰,史冬玲,等.麦冬皂苷B抗肿瘤作用机制研究进展.中华中医药学刊,2018,36(12):2911-2914
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[14]林振吕,郑光威,张琳,等.蝎毒多肽对胃癌SGC-7901细胞侵袭转移的作用.中国临床药理学杂志,2016,32(13):1206-1209
[15]Chu D,Zhang Z X,Li Y M,et al. Matrix metalloproteinase-9 is associated with disease-free survival and overall survival in patients with gastric cancer.Int J Cancer,2011,129(4):887-895
[16]王曼,朱振红,朱正秋.MACC1调节肝星状细胞表达MMP-2、MMP-9对胃癌细胞迁移侵袭能力的影响.胃肠病学和肝病学杂志,2016,25(07):765-768
[17]杨云鹏,刘莉,李慧.MMP-2、MMP-3和MMP-9在胃癌患者中的表达及意义.局解手术学杂志,2016,25(9):625-628
[18]Burlaka A P,Ganusevich I I,Gafurov M R,et al.Stomach Cancer:Interconnection between the Redox State,Activity of MMP-2,MMP-9 and Stage of Tumor Growth.Cancer Microenvironment,2016,9(1):27-32
[2]郑朝旭,郑荣寿,张思维,等.中国2010年胃癌发病与死亡分析.中国肿瘤,2014,23(10):795-800
[3]李炜伟,唐岱,王振冉.胃癌侵袭转移机制的研究现状及进展.当代医学,2018,24(36):190-193
[4]国家药典委员会.中华人民共和国药典.一部.北京:中国医药科技出版社,2015
[5]彭婉,马骁,王建,等.麦冬化学成分及药理作用研究进展.中草药,2018,49(2):477-488
[6]陈美娟,赵若琳,郭园园,等.麦冬皂苷B对A549细胞株体外黏附、侵袭及迁移的抑制作用及机制研究.中国药理学通报,2015,31(5):660-664
[7]周志红.麦冬皂苷B诱导人肝癌HepG2细胞自噬的分子机制研究.北方药学,2017,14(9):156-157,174
[8]许秋菊,侯莉莉,胡国强,等.麦冬皂苷B诱导人宫颈癌He La细胞自噬的机制.药学学报,2013,48(6):855-859
[9]Lee S Y,Chung S M.Neovastat(AE 941)inhibits the airway inflammation via VEGF and HIF-2A suppression.Vascul Pharmacol,2007,47(5/6):313-318
[10]赵益,王高玉,戈淑超,等.中医药对胃癌预防的研究进展.中华中医药杂志,2017,32(3):1162-1164
[11]刘磊,张光霁,徐楚韵,等.解毒三根汤痰毒同治抗消化道肿瘤作用初探.中华中医药杂志,2018,33(11):4824-4826
[12]何冰倩,周小杰,史冬玲,等.麦冬皂苷B抗肿瘤作用机制研究进展.中华中医药学刊,2018,36(12):2911-2914
[13]Huang S,Ingber D E.A non-genetic basis for cancer progression and metastasis:Self-organizing attractors in cell regulatory networks.Breast Dis,2006,26(1):27-54
[14]林振吕,郑光威,张琳,等.蝎毒多肽对胃癌SGC-7901细胞侵袭转移的作用.中国临床药理学杂志,2016,32(13):1206-1209
[15]Chu D,Zhang Z X,Li Y M,et al. Matrix metalloproteinase-9 is associated with disease-free survival and overall survival in patients with gastric cancer.Int J Cancer,2011,129(4):887-895
[16]王曼,朱振红,朱正秋.MACC1调节肝星状细胞表达MMP-2、MMP-9对胃癌细胞迁移侵袭能力的影响.胃肠病学和肝病学杂志,2016,25(07):765-768
[17]杨云鹏,刘莉,李慧.MMP-2、MMP-3和MMP-9在胃癌患者中的表达及意义.局解手术学杂志,2016,25(9):625-628
[18]Burlaka A P,Ganusevich I I,Gafurov M R,et al.Stomach Cancer:Interconnection between the Redox State,Activity of MMP-2,MMP-9 and Stage of Tumor Growth.Cancer Microenvironment,2016,9(1):27-32
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