叶面喷施1-MCP缓解辣椒幼苗高温伤害的机理研究
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摘要
以‘纽内姆黄帅’辣椒为试验材料,研究高温胁迫下叶面喷施1–甲基环丙烯(1-MCP)缓解幼苗伤害的可能机理。结果表明,高温胁迫对辣椒幼苗的生长具有明显的抑制作用,热害指数高达60.5。与叶面喷施清水对照相比,叶面喷施80 g·hm~(-2) 1-MCP显著提高了高温胁迫下幼苗生物量、根系活力、叶绿素含量、净光合速率和叶片抗氧化酶活性,增加了叶片可溶性蛋白和可溶性糖的含量,有效抑制活性氧积累并降低细胞膜透性,而且显著降低了叶片中脱落酸含量和乙烯释放速率,提高了辣椒坐果率和单果质量。表明叶面喷施适宜浓度的1-MCP可通过提高辣椒光合作用、增强渗透调节活性、抑制活性氧积累、调节激素含量等,缓解高温胁迫对辣椒造成的伤害。
Capsicum annuum L.‘Niuneimu Huangshuai'used as experimental material,the possible mechanism of foliar-spraying 1-methylcyclopropene(1-MCP)alleviating the injury of pepper seedlings under high temperature stress was studied. The results showed that the growth of pepper seedlings was significantly suppressed by high temperature stress,and the heat injury index was reached up to 60.5. The biomass,root activity,chlorophyll content,net photosynthetic rate,leaf antioxidant enzymes activities,the contents of soluble protein and soluble sugar of pepper seedlings were significantly promoted by foliar-spraying 80 g · hm~(-2) 1-MCP compared with the treatment of foliar-spraying water. Moreover,the accumulation of reactive oxygen species and cell membrane permeability of pepper seedlings treated with foliar-spraying 80 g · hm~(-2) 1-MCP under high temperature were significantly decreased compared with the treatment of foliar-spraying water. In addition,the abscisic acid content and ethylene release rate in pepper leaves treated with 1-MCP under high temperature stress was decreased,on the contrary,fruit setting and yield per plant were effectively improved compared with the treatment of foliar-spraying water. In conclusion,the results indicated that foliar-applied suitable concentration of 1-MCP on pepper seedlings under high temperature stress could enhance photosynthesis rate and osmotic adjustment activity,inhibited the accumulation of reactive oxygen species,and regulate the hormone contents,as a result,improved the yield of peppers.
Capsicum annuum L.‘Niuneimu Huangshuai'used as experimental material,the possible mechanism of foliar-spraying 1-methylcyclopropene(1-MCP)alleviating the injury of pepper seedlings under high temperature stress was studied. The results showed that the growth of pepper seedlings was significantly suppressed by high temperature stress,and the heat injury index was reached up to 60.5. The biomass,root activity,chlorophyll content,net photosynthetic rate,leaf antioxidant enzymes activities,the contents of soluble protein and soluble sugar of pepper seedlings were significantly promoted by foliar-spraying 80 g · hm~(-2) 1-MCP compared with the treatment of foliar-spraying water. Moreover,the accumulation of reactive oxygen species and cell membrane permeability of pepper seedlings treated with foliar-spraying 80 g · hm~(-2) 1-MCP under high temperature were significantly decreased compared with the treatment of foliar-spraying water. In addition,the abscisic acid content and ethylene release rate in pepper leaves treated with 1-MCP under high temperature stress was decreased,on the contrary,fruit setting and yield per plant were effectively improved compared with the treatment of foliar-spraying water. In conclusion,the results indicated that foliar-applied suitable concentration of 1-MCP on pepper seedlings under high temperature stress could enhance photosynthesis rate and osmotic adjustment activity,inhibited the accumulation of reactive oxygen species,and regulate the hormone contents,as a result,improved the yield of peppers.
引文
Da Costa V A,Cothren J T,Bynum J B.2011.Abiotic stress effects on plant growth and yield components of 1-MCP treated cotton plants.Agronomy Journal,103(6):1591-1596.
Djanaguiraman M,Boyle D L,Welti R.2018.Decreased photosynthetic rate under high temperature in wheat is due to lipid desaturation,oxidation,acylation,and damage of organelles.BMC Plant Biology,18(1):55.
Djanaguiraman M,Prasad P V V.2010.Ethylene production under high temperature stress causes premature leaf senescence in soybean.Functional Plant Biology,37(11):1071-1084.
Djanaguiraman M,Prasad P V V,AI-Khatib K.2011.Ethylene perception inhibitor 1-MCP decreases oxidative damage of leaves through enhanced antioxidant defense mechanisms in soybean plants grown under high temperature stress.Environmental and Experimental Botany,71(2):215-223.
Guo Yang-dong,Zhang Lei,Li Shuang-tao,Cao Yun-yun,Qi Chuan-dong,Wang Jin-fang.2018.Progresses in research on molecular biology of abiotic stress responses in vegetable crops.Scientia Agricultura Sinica,51(6):1167-1181.(in Chinese)郭仰东,张磊,李双桃,曹芸运,齐传东,王晋芳.2018.蔬菜作物应答非生物逆境胁迫的分子生物学研究进展.中国农业科学,51(6):1167-1181.
Guo Yun-na,Li Yan-su,He Chao-xing,Yu Xian-chang.2015.Effects of sodium naphthalene acetate on growth and physiological characteristics of tomato seedlings under suboptimal temperature and light condition.Chinese Journal of Applied Ecology,26(10):3053-3058.(in Chinese)郭允娜,李衍素,贺超兴,于贤昌.2015.亚适宜温光下萘乙酸钠对番茄幼苗生长与生理特性的影响.应用生态学报,26(10):3053-3058.
Hays D B,Do J H,Mason R E,Morgan G,Finlayson S A.2007.Heat stress induced ethylene production in developing wheat grains induces kernel abortion and increased maturation in a susceptible cultivar.Plant Science,172(6):1113-1123.
Hershkovitz V,Friedman H,Goldschmidt E E.2009.Induction of ethylene in avocado fruit in response to chilling stress on tree.Plant Physiology,166(17):1855-1862.
Kao C H,Yang S F.1983.Role of ethylene in the senescence of detached rice leaves.Plant Physiology,73(4):881-885.
Kawakami E M,Oosterhuis D M,Snider J L.2010.Physiological effects of 1-Methylcyclopropene on well-watered and water-stressed cotton plants.Journal of Plant Growth Regulation,29(3):280-288.
Khan A S,Singh Z.2007.1-MCP regulates ethylene biosynthesis and fruit softening during ripening of‘Tegan Blue’plum.Postharvest Biology Technology,43(3):298-306.
Kurepin L V,Ivanov A G,Zaman M,Pharis R P,Allakhverdiev S I.2015.Stress-related hormones and glycinebetaine interplay in protection of photosynthesis under abiotic stress conditions.Photosynthesis Research,126(2-3):1-15.
Larrigaudiere C,Vilaplana R,Soria Y,Recasens I.2004.Oxidative behavior of Blanquilla pears treated with 1-methylcyclopropene during cold storage.Journal of the Science of Food and Agriculture,84(14):1871-1877.
Li Li-jie,Gu Wan-rong,Meng Yao,Wang Yue-li.2018.Physiological and biochemical mechanism of spermidine improving drought resistance in maize seedlings under drought stress.Chinese Journal of Applied Ecology,29(2):554-564.(in Chinese)李丽杰,顾万荣,孟瑶,王悦力.2018.干旱胁迫下亚精胺对玉米幼苗抗旱性影响的生理生化机制.应用生态学报,29(2):554-564.
Lina D,Jun S,Leslie C P,Sherry F,Zhao Q Z.2017.Quantitative proteomic changes in development of superficial scald disorder and its response to diphenylamine and 1-MCP treatments in apple fruit.Postharvest Biology and Technology,123:33-50.
McClung C R,Davis S J.2010.Ambient thermometers in plants:from physiological outputs towards mechanisms of thermal sensing.Current Biology,20(24):1086-1092.
Mohammed A R,Cothren J T,Chen M H,arpley L.2015.1-Methylcyclopropene(1-MCP)-induced alteration in leaf photosynthetic rate,chlorophyll fluorescence,respiration and membrane damage in rice(Oryza sativa L.)under high night temperature.Journal of Agronomy and Crop Science,201(2):105-116.
Narayana I,Lalonde S,Saini H S.1991.Water-stress-induced ethylene production in wheat:a fact or artifact.Plant Physiology,96(2):406-410.
Nie Wen-jing,Wang Shuo-shuo,Jing Xin,Gong Biao,Wei Min,Yang Feng-juan,Li Yan,Shi Qing-hua.2018.Effects of exogenous 2,4-epibrassinolide on the growth and redox balance of cucumber seedlings under NaHCO3 stress.Chinese Journal of Applied Ecology,29(3):899-908.(in Chinese)聂文婧,王硕硕,荆鑫,巩彪,魏珉,杨凤娟,李岩,史庆华.2018.外源表油菜素内酯对NaHCO3胁迫下黄瓜幼苗生长及氧化还原平衡的影响.应用生态学报,29(3):899-908.
Ruelland E,Zachowski A.2010.How plants sense temperature.Environmental Experimental Botany,69(3):225-232.
Sabban-Amin R,Feygenberg O,Belausov E,Pesis E.2011.Low oxygen and 1-MCP pretreatments delay superficial scald development by reducing reactive oxygen species(ROS)accumulation in stored‘Granny Smith’apples.Postharvest Biology and Technology,62(3):295-304.
Sun Ke-xiang,Yang Sha,Guo Feng,Liu Cui-min,Meng Jing-jing.2015.Effects of exogenous calcium on photosynthetic characteristics of sweet pepper(Capsicum fructescens L.)seedlings.Plant Physiology Journal,51(3):280-286.(in Chinese)孙克香,杨莎,郭峰,刘翠敏,孟静静.2015.高温强光胁迫下外源钙对甜椒(Capsicum fructescens L.)幼苗光合生理特性的影响.植物生理学报,51(3):280-286.
Sun Sheng-nan.2017.Response and adaptation of protected cucumber to high temperature stress[M.D.Dissertation].Tai’an:Shandong Agricultural University.(in Chinese)孙胜楠.2017.设施黄瓜对高温胁迫的响应与适应[硕士论文].泰安:山东农业大学.
Suzuki N,Koussevitzky S,Mittler R,Miller G.2012.ROS and redox signalling in the response of plants to abiotic stress.Plant Cell and Environment,35(2):259-270.
Watkins C B.2006.The use of 1-methylcyclopropene(1-MCP)on fruits and vegetables.Biotechnology Advances,24(4):389-409.
Weihong H,Sun D W,Jose B.2017.Rapid monitoring 1-MCP-induced modulation of sugars accumulation in ripening‘Hayward’kiwifruit by Vis/NIR hyperspectral imaging.Postharvest Biology and Technology,125:168-180.
Yang Qing,Guo Shou-hua.2010.Plant Physiology and Biochemistry.Beijing:China Agricultural Science and Technology Press:111-112,128-129.(in Chinese)杨晴,郭守华.2010.植物生理生化.北京:中国农业科学技术出版社:111-112,128-129.
Yin X R,Xie X L,Xia X J,Yu J Q,Ian B F,James J G,Chen K S.2016.Involvement of an ethylene response factor in chlorophyll degradation during citrus fruit degreening.The Plant Journal,86(5):403-412.
Djanaguiraman M,Boyle D L,Welti R.2018.Decreased photosynthetic rate under high temperature in wheat is due to lipid desaturation,oxidation,acylation,and damage of organelles.BMC Plant Biology,18(1):55.
Djanaguiraman M,Prasad P V V.2010.Ethylene production under high temperature stress causes premature leaf senescence in soybean.Functional Plant Biology,37(11):1071-1084.
Djanaguiraman M,Prasad P V V,AI-Khatib K.2011.Ethylene perception inhibitor 1-MCP decreases oxidative damage of leaves through enhanced antioxidant defense mechanisms in soybean plants grown under high temperature stress.Environmental and Experimental Botany,71(2):215-223.
Guo Yang-dong,Zhang Lei,Li Shuang-tao,Cao Yun-yun,Qi Chuan-dong,Wang Jin-fang.2018.Progresses in research on molecular biology of abiotic stress responses in vegetable crops.Scientia Agricultura Sinica,51(6):1167-1181.(in Chinese)郭仰东,张磊,李双桃,曹芸运,齐传东,王晋芳.2018.蔬菜作物应答非生物逆境胁迫的分子生物学研究进展.中国农业科学,51(6):1167-1181.
Guo Yun-na,Li Yan-su,He Chao-xing,Yu Xian-chang.2015.Effects of sodium naphthalene acetate on growth and physiological characteristics of tomato seedlings under suboptimal temperature and light condition.Chinese Journal of Applied Ecology,26(10):3053-3058.(in Chinese)郭允娜,李衍素,贺超兴,于贤昌.2015.亚适宜温光下萘乙酸钠对番茄幼苗生长与生理特性的影响.应用生态学报,26(10):3053-3058.
Hays D B,Do J H,Mason R E,Morgan G,Finlayson S A.2007.Heat stress induced ethylene production in developing wheat grains induces kernel abortion and increased maturation in a susceptible cultivar.Plant Science,172(6):1113-1123.
Hershkovitz V,Friedman H,Goldschmidt E E.2009.Induction of ethylene in avocado fruit in response to chilling stress on tree.Plant Physiology,166(17):1855-1862.
Kao C H,Yang S F.1983.Role of ethylene in the senescence of detached rice leaves.Plant Physiology,73(4):881-885.
Kawakami E M,Oosterhuis D M,Snider J L.2010.Physiological effects of 1-Methylcyclopropene on well-watered and water-stressed cotton plants.Journal of Plant Growth Regulation,29(3):280-288.
Khan A S,Singh Z.2007.1-MCP regulates ethylene biosynthesis and fruit softening during ripening of‘Tegan Blue’plum.Postharvest Biology Technology,43(3):298-306.
Kurepin L V,Ivanov A G,Zaman M,Pharis R P,Allakhverdiev S I.2015.Stress-related hormones and glycinebetaine interplay in protection of photosynthesis under abiotic stress conditions.Photosynthesis Research,126(2-3):1-15.
Larrigaudiere C,Vilaplana R,Soria Y,Recasens I.2004.Oxidative behavior of Blanquilla pears treated with 1-methylcyclopropene during cold storage.Journal of the Science of Food and Agriculture,84(14):1871-1877.
Li Li-jie,Gu Wan-rong,Meng Yao,Wang Yue-li.2018.Physiological and biochemical mechanism of spermidine improving drought resistance in maize seedlings under drought stress.Chinese Journal of Applied Ecology,29(2):554-564.(in Chinese)李丽杰,顾万荣,孟瑶,王悦力.2018.干旱胁迫下亚精胺对玉米幼苗抗旱性影响的生理生化机制.应用生态学报,29(2):554-564.
Lina D,Jun S,Leslie C P,Sherry F,Zhao Q Z.2017.Quantitative proteomic changes in development of superficial scald disorder and its response to diphenylamine and 1-MCP treatments in apple fruit.Postharvest Biology and Technology,123:33-50.
McClung C R,Davis S J.2010.Ambient thermometers in plants:from physiological outputs towards mechanisms of thermal sensing.Current Biology,20(24):1086-1092.
Mohammed A R,Cothren J T,Chen M H,arpley L.2015.1-Methylcyclopropene(1-MCP)-induced alteration in leaf photosynthetic rate,chlorophyll fluorescence,respiration and membrane damage in rice(Oryza sativa L.)under high night temperature.Journal of Agronomy and Crop Science,201(2):105-116.
Narayana I,Lalonde S,Saini H S.1991.Water-stress-induced ethylene production in wheat:a fact or artifact.Plant Physiology,96(2):406-410.
Nie Wen-jing,Wang Shuo-shuo,Jing Xin,Gong Biao,Wei Min,Yang Feng-juan,Li Yan,Shi Qing-hua.2018.Effects of exogenous 2,4-epibrassinolide on the growth and redox balance of cucumber seedlings under NaHCO3 stress.Chinese Journal of Applied Ecology,29(3):899-908.(in Chinese)聂文婧,王硕硕,荆鑫,巩彪,魏珉,杨凤娟,李岩,史庆华.2018.外源表油菜素内酯对NaHCO3胁迫下黄瓜幼苗生长及氧化还原平衡的影响.应用生态学报,29(3):899-908.
Ruelland E,Zachowski A.2010.How plants sense temperature.Environmental Experimental Botany,69(3):225-232.
Sabban-Amin R,Feygenberg O,Belausov E,Pesis E.2011.Low oxygen and 1-MCP pretreatments delay superficial scald development by reducing reactive oxygen species(ROS)accumulation in stored‘Granny Smith’apples.Postharvest Biology and Technology,62(3):295-304.
Sun Ke-xiang,Yang Sha,Guo Feng,Liu Cui-min,Meng Jing-jing.2015.Effects of exogenous calcium on photosynthetic characteristics of sweet pepper(Capsicum fructescens L.)seedlings.Plant Physiology Journal,51(3):280-286.(in Chinese)孙克香,杨莎,郭峰,刘翠敏,孟静静.2015.高温强光胁迫下外源钙对甜椒(Capsicum fructescens L.)幼苗光合生理特性的影响.植物生理学报,51(3):280-286.
Sun Sheng-nan.2017.Response and adaptation of protected cucumber to high temperature stress[M.D.Dissertation].Tai’an:Shandong Agricultural University.(in Chinese)孙胜楠.2017.设施黄瓜对高温胁迫的响应与适应[硕士论文].泰安:山东农业大学.
Suzuki N,Koussevitzky S,Mittler R,Miller G.2012.ROS and redox signalling in the response of plants to abiotic stress.Plant Cell and Environment,35(2):259-270.
Watkins C B.2006.The use of 1-methylcyclopropene(1-MCP)on fruits and vegetables.Biotechnology Advances,24(4):389-409.
Weihong H,Sun D W,Jose B.2017.Rapid monitoring 1-MCP-induced modulation of sugars accumulation in ripening‘Hayward’kiwifruit by Vis/NIR hyperspectral imaging.Postharvest Biology and Technology,125:168-180.
Yang Qing,Guo Shou-hua.2010.Plant Physiology and Biochemistry.Beijing:China Agricultural Science and Technology Press:111-112,128-129.(in Chinese)杨晴,郭守华.2010.植物生理生化.北京:中国农业科学技术出版社:111-112,128-129.
Yin X R,Xie X L,Xia X J,Yu J Q,Ian B F,James J G,Chen K S.2016.Involvement of an ethylene response factor in chlorophyll degradation during citrus fruit degreening.The Plant Journal,86(5):403-412.